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941.
Gemma N. Villagomez Fabian Nürnberger Fabrice Requier Susanne Schiele Ingolf SteffanDewenter 《Ecology and evolution》2021,11(12):7834
Temperature and photoperiod are important Zeitgebers for plants and pollinators to synchronize growth and reproduction with suitable environmental conditions and their mutualistic interaction partners. Global warming can disturb this temporal synchronization since interacting species may respond differently to new combinations of photoperiod and temperature under future climates, but experimental studies on the potential phenological responses of plants and pollinators are lacking. We simulated current and future combinations of temperature and photoperiod to assess effects on the overwintering and spring phenology of an early flowering plant species (Crocus sieberi) and the Western honey bee (Apis mellifera). We could show that increased mean temperatures in winter and early spring advanced the flowering phenology of C. sieberi and intensified brood rearing activity of A. mellifera but did not advance their brood rearing activity. Flowering phenology of C. sieberi also relied on photoperiod, while brood rearing activity of A. mellifera did not. The results confirm that increases in temperature can induce changes in phenological responses and suggest that photoperiod can also play a critical role in these responses, with currently unknown consequences for real‐world ecosystems in a warming climate. 相似文献
942.
Fabian Chen 《The Yale journal of biology and medicine》1986,59(3):375-Jun;59(3):375
943.
Summary Ecological species replacement of Liolaemus platei by Liolaemus lemniscatus appears to occur along a north-south oriented herb density gradient. Both congeners are the only ground-dwelling lizards in the area; this suggests that L. lemniscatus is an ecological counterpart of L. platei in herbaceous habitats. Relatively longer legs appear to be associated with the utilization of herb-free habitats by L. platei; the shorter legs possessed by L. lemniscatus are associated with the utilization of habitats of higher herb density. Morphological evidence indicates the presence of intermediate populations in the zone of intermediate herb density. Alternatives of a single-species cline versus interspecific hybridization between the two taxa are discussed. 相似文献
944.
Best practices in heterotrophic high-cell-density microalgal processes: achievements,potential and possible limitations 总被引:1,自引:0,他引:1
Bumbak F Cook S Zachleder V Hauser S Kovar K 《Applied microbiology and biotechnology》2011,91(1):31-46
Microalgae of numerous heterotrophic genera (obligate or facultative) exhibit considerable metabolic versatility and flexibility
but are currently underexploited in the biotechnological manufacturing of known plant-derived compounds, novel high-value
biomolecules or enriched biomass. Highly efficient production of microalgal biomass without the need for light is now feasible
in inexpensive, well-defined mineral medium, typically supplemented with glucose. Cell densities of more than 100 g l−1 cell dry weight have been achieved with Chlorella, Crypthecodinium and Galdieria species while controlling the addition of organic sources of carbon and energy in fedbatch mode. The ability of microalgae
to adapt their metabolism to varying culture conditions provides opportunities to modify, control and thereby maximise the
formation of targeted compounds with non-recombinant microalgae. This review outlines the critical aspects of cultivation
technology and current best practices in the heterotrophic high-cell-density cultivation of microalgae. The primary topics
include (1) the characteristics of microalgae that make them suitable for heterotrophic cultivation, (2) the appropriate chemical
composition of mineral growth media, (3) the different strategies for fedbatch cultivations and (4) the principles behind
the customisation of biomass composition. The review confirms that, although fundamental knowledge is now available, the development
of efficient, economically feasible large-scale bioprocesses remains an obstacle to the commercialisation of this promising
technology. 相似文献
945.
Nele Verhulst Fabian Kienle Ken D. Sayre Jozef Deckers Dirk Raes Agustin Limon-Ortega Leonardo Tijerina-Chavez Bram Govaerts 《Plant and Soil》2011,340(1-2):453-466
There is a clear need to develop conservation agriculture technologies appropriate for surface irrigated conditions, and the adoption by small-scale farmers. The objective of this paper is to evaluate the effect on soil quality of five different tillage-crop residue management systems (conventionally tilled raised beds [CTB] with residue incorporated and permanent raised beds [PB] with residue burned, removed, partly retained or fully retained). Data were collected in a long-term trial established in 1992 with a wheat-maize rotation under irrigated, arid conditions in north-western Mexico. Three groups of tillage-straw systems with different characteristics in relation to the soil environment were distinguished: PB-straw burned, CTB-straw incorporated, and PB-straw not burned. The PB-straw burned had high electrical conductivity, Na concentration and penetration resistance and low soil resilience and aggregation, showing that the combination of PB with the burning of residues is not a sustainable management option. The CTB-straw incorporated was distinguished from the PB practices by the soil physical variables, especially the low direct infiltration and aggregate stability, indicating degradation of physical soil quality in this system. The practice of PB, where all or part of the residue is retained in the field, seems to be the most sustainable option for this cropping system. 相似文献
946.
Engel S Heger T Mancini R Herzog F Kartenbeck J Hayer A Helenius A 《Journal of virology》2011,85(9):4198-4211
After binding to its cell surface receptor ganglioside GM1, simian virus 40 (SV40) is endocytosed by lipid raft-mediated endocytosis and slowly transported to the endoplasmic reticulum, where partial uncoating occurs. We analyzed the intracellular pathway taken by the virus in HeLa and CV-1 cells by using a targeted small interfering RNA (siRNA) silencing screen, electron microscopy, and live-cell imaging as well as by testing a variety of cellular inhibitors and other perturbants. We found that the virus entered early endosomes, late endosomes, and probably endolysosomes before reaching the endoplasmic reticulum and that this pathway was part of the infectious route. The virus was especially sensitive to a variety of perturbations that inhibited endosome acidification and maturation. Contrary to our previous models, which postulated the passage of the virus through caveolin-rich organelles that we called caveosomes, we conclude that SV40 depends on the classical endocytic pathway for infectious entry. 相似文献
947.
Schmökel J Sauter D Schindler M Leendertz FH Bailes E Dazza MC Saragosti S Bibollet-Ruche F Peeters M Hahn BH Kirchhoff F 《Journal of virology》2011,85(2):742-752
Nef is an accessory protein critical for the ability of human and simian immunodeficiency viruses (HIV and SIV) to replicate efficiently in their respective hosts. Previous analyses of members of 15 different primate lentivirus lineages revealed a link between Nef function and the presence of a vpu gene. In particular, Nef proteins of all vpu-containing viruses had lost their ability to downmodulate the T cell (TCR-CD3) receptor. Here we examined Nef proteins from eight additional SIV lineages, including SIVgor, SIVwrc, SIVolc, SIVgri, SIVdrl, SIVlho, SIVden, and SIVasc, from western lowland gorillas, western red colobus monkeys, olive colobus monkeys, grivet monkeys, drills, L'Hoest's monkeys, Dent's mona monkeys, and red-tailed monkeys, respectively. We found that except for the nef gene of SIVdrl, all of them were efficiently expressed and modulated CD4, major histocompatibility complex class I (MHC-I), CD28, CXCR4, and Ii cell surface expression and/or enhanced viral infectivity and replication. Furthermore, the Nef proteins of SIVgri, SIVlho, SIVwrc, SIVolc, and SIVgor antagonized tetherin. As expected, the Nef protein of SIVgor, which carries vpu, failed to downmodulate CD3, whereas those of SIVwrc, SIVgri, SIVlho, and SIVasc, which lack vpu, were capable of performing this function. Surprisingly, however, the Nef protein of the vpu-containing SIVden strain retained the ability to downmodulate TCR-CD3, whereas that of SIVolc, which does not contain vpu, was unable to perform this function. Although the SIVden Vpu is about 20 amino acids shorter than other Vpu proteins, it degrades CD4 and antagonizes tetherin. Our data show that there are exceptions to the link between the presence of a vpu gene and nef alleles deficient in CD3 modulation, indicating that host properties also affect the selective pressure for Nef-mediated disruption of TCR-CD3 signaling. Our results are also further evidence that tetherin antagonism is a common function of primate lentivirus Nef proteins and that the resistance of human tetherin to Nef represents a relevant barrier to cross-species transmission of SIVs to humans. 相似文献
948.
Suárez-Ortegón MF Jiménez P Mosquera M Pradilla AG Gracia AB Aguilar de Plata C 《Biological trace element research》2011,144(1-3):445-453
Copper and calcium are essential for human growth and development. The present study was conducted to evaluate the relationship between calcium and copper levels, as well as their relationship to age and anthropometry in 180 preschool children ages 2-5?years old. Serum copper levels were inversely correlated with age (r?=?-0.184, P?=?0.032) and height (r?=?-0.175, P?=?0.043) in the whole group and with height for age Z score only in male children (r?=?-0.291, P?=?0.016). The correlation with age is not maintained when it is analyzed for gender. Serum calcium values in the total group were inversely correlated with weight (r?=?-0.153, P?=?0.044) and weight for height Z score (r?=?-0.246, P?=?0.001). No differences were found for gender in the levels of both metals. A negative relationship between serum calcium and copper was found only in male children (r?=?-0.339, P?=?0.005). Studies are required in other populations and experimental designs that can explain an inverse relationship between serum calcium and copper levels. 相似文献
949.
Signaling networks are abundant in higher organisms. They play pivotal roles, e.g., during embryonic development or within
the immune system. In this contribution, we study the combined effect of the various kinetic parameters on the dynamics of
signal transduction. To this end, we consider hierarchical complex systems as prototypes of signaling networks. For given
topology, the output of these networks is determined by an interplay of the single parameters. For different kinetics, we
describe this by algebraic expressions, the so-called effective parameters. 相似文献
950.
The activity of eIF4A, a key player in translation initiation, is regulated by other translation factors through currently unknown mechanisms. Here, we provide the necessary framework to understand the mechanism of eIF4A's regulation by eIF4G. In solution, eIF4A adopts a defined conformation that is different from the crystal structure. Binding of eIF4G induces a 'half-open' conformation by interactions with both domains, such that the helicase motifs are pre-aligned for activation. A primary interface acts as an anchor for complex formation. We show here that formation of the secondary interface is essential for imposing the 'half-open' conformation on eIF4A, and it is critical for the functional interaction of eIF4G with eIF4A. Via this bipartite interaction, eIF4G guides the transition of eIF4A between the 'half-open' and closed conformations, and stimulates its activity by accelerating the rate-limiting step of phosphate release. Subtle changes induced by eIF4G may be amplified by input signals from other translation factors, leading to an efficient regulation of translation initiation. 相似文献