Some biochemical indices in chicken skin tissue during ontogenesis and under the effect of ionizing radiation

The research shows some biochemical indices in ontogenesis in broiler hen skin tissues and also its reaction to inner introduction of different doses of radioactive 137Cs. Namely, the irradiation ionizing irradiation influences actively to the indices of nuclein changes with 1 day and 1 week aged chickens. The quantity of phosphorus of nuclein acids is less with the chickens of the third group (500 Bk pr day) than with the second group (3000 Bk/day). The quantity of soluble protein, activity of the estimated fragments, the quantity of inorganic phosphorus, the concentration of ions of magnesium with chickens of the controlled group is larger than in the studied ones. It proves the influence of radionucleid 137Cs on indices which characterize the protein-nuclein change.     

Characteristics of some stages of energy metabolism and antioxidant system in bone marrow myeloid cells and leukocytes from piglets

The age changes of enzymes of activity catalyzing several links of energy metabolism (hexokinase, phosphofructokinase, pyruvate kinase, lactate dehydrogenase, glucose-6-phosphate dehydrogenase, NADP-isocitrate dehydrogenase, cytochrome c-oxidase) and antioxidant system (superoxide dismutase and glutathione reductase) in bone marrow myeloid cells and blood leukocytes of pig in the 10-day period after birth were investigated. The bone marrow cells and leukocytes of the new born piglets were characterized by low intensity of oxidative steps of energy metabolism as well as by low activity of antioxidant enzymes. In the period of neonatal adaptation reorganization of energy metabolism, particularly, intensification of oxidative processes in the investigated cells occurred. It included the pentose phosphate way and cytochrome c-oxidase activation. During the neonatal period of development the functional activity of antioxidant enzymes in the investigated cells of piglets increased.     

Immune sensor for determining myoglobin level

For the first time, the photoluminescence of porous silicon was used to create an immune sensor capable of detection of low concentrations of myoglobin important for the diagnostics of heart failure diseases which in both model solutions and human serum, being. It is shown that the sensitivity of the sensor was 10 ng/ml, time of 1 measurement--15-30 min. Duration of the analysis can be even shorter if measurements are performed in the kinetic regime. The comparison of the characteristics of the immune sensor and standard method ELISA is also given in the paper.     

Antioxidant properties and membranotropic effect of certain derivatives of 1,4-dihydropyridine

Interaction of alpha-tocopherol and 1,4-dihydropyridine with endoplasmic reticulum membranes and model systems, human serum albumin and phospholipid bilayer, was studied using the microcalorimetry and fluorescent probes procedures. Dependence of microviscosity changes in the endoplasmic reticulum membranes on the place of antioxidants localization (protein structures or phospholipid phase) was shown. Increase of membrane structuralization under the influence of 1,4-dihydropyridines blocked their antioxidant action in spontaneous and induced lipid peroxidation.