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ISABELLE TARDIEUX OLIVIER POUPEL FRANCOIS RODHAIN LAURENT LAPCHIN 《Medical and veterinary entomology》1992,6(4):311-317
Female Aedes albopictus mosquitoes, aged 1 week, were infected with DEN-2 dengue virus. The kinetics of infection in mosquito brain and mesenteron were monitored using DNA probes with polymerase chain reaction (PCR) amplification of target DNA sequences coding for DEN-2 virus envelope protein, compared with the standard immunofluorescence assay technique (IFA). Rates of virus detection in the mesenteron of orally infected mosquitoes rose to 38% by day 4 post-inoculation, then declined until day 8, followed by irregular peaks around days 11-14 and subsequently. In mosquito head squashes, virus was detected from day 4 onwards, reaching 38% positive by day 18. Salivary glands of all the same females were found to be positive for virus by day 8 onwards. Parenterally infected Ae.albopictus females were all positive for DEN-2 in the brain and salivary glands 8 days post-inoculation. In every case, results obtained with the PCR matched those from the IFA. Our DNA probe with PCR procedure can therefore be utilized as a sensitive and reliable method for studies of DEN-2 vectors. 相似文献
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Heat Stress-induced Abortion of Buds and Flowers in Pea: Is Sensitivity Linked to Organ Age or to Relations between Reproductive Organs? 总被引:5,自引:0,他引:5
A short period of heat stress can cause a significant increasein abortion frequency of floral buds and flowers in pea, buta large variability in sensitivity exists among reproductiveorgans (ROs) within a plant or between plants. We have analysedspatial distribution of abortion frequency in plants subjectedor not subjected to heat stress in: (1) four controlled environmentexperiments in which apex temperature was increased to 31 °Cfor 6 h per day for 4 d (moderate stress); (2) one field experimentwith a similar level of stress; and (3) one experiment with2 d with 33/30 °C day/night (severe stress) in additionto treatment (1). Influence of neighbouring organs on sensitivityto heat stress was analysed by partial flower removal. Severeheat stress caused rapid interruption of RO development followedby abscission while, in moderate stress, at leat 4 d elapsedbetween cessation of stress and first symptoms of abortion.In both moderate stress and control treatments, abortion frequencyfollowed a consistent pattern along the stem with no abortionon the lowest reproductive phytomeres, a temperature-dependentabortion frequency on intermediate phytomeres and 100% abortionon apical phytomeres. Regardless of temperature, this patternwas shifted by four positions if ROs were removed on the lowestfour reproductive phytomeres, and no abortion was observed ifyoung pods were sequentially removed. Moderate heat stress increasedthe effect of developing ROs on abortion frequency of youngerROs located above them. We suggest that the effect of mild stressshould be viewed as the acceleration of a programme linked tothe normal termination of phytomere production during the plantcycle, rather than as an abrupt event linked to stress. Pisum sativum; heat stress; abortion; abscission; age; organ-to-organ relations 相似文献