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41.
Comparison of Single and Touchdown PCR Protocols for Detecting Pneumocystis jirovecii DNA in Paraffin-Embedded Lung Tissue Samples 总被引:1,自引:0,他引:1
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Abstract: Dercetid fishes are common in Tethyan marine deposits of Europe, Asia and Africa. In this paper, we describe a South American dercetid fish, Brazilodercetis longirostris gen. et sp. nov., based on juvenile and adult specimens found in core samples at c. 4000 m sediment depth in the Atlântida Formation (Pelotas Basin, southern Brazil). Brazilodercetis longirostris is a slender, elongate fish recognized on the basis of the following features: prominent snout; contact between mesethmoid and frontals very anterior to the orbit; mesethmoid separating the premaxillae for half of their length; juvenile specimens bearing wedge-like teeth on the anterior third of maxilla; long and shallow crest crossing epioccipital, parietal and posterior border of frontal; a pipe-like preopercle; and reduced neural spine at the middle point of neural arch. Brazilodercetis shares a number of features with the other dercetids, including a pointed snout that is longer than the lower jaw (with Rhynchodercetis and Hastichthys ), a medioparietal skull roof (with Cyranichthys and Benthesikyme ), an unroofed posttemporal fossa (with Dercetoides , Rhynchodercetis and Pelargorhynchus ), a flange on the anguloarticular (with Dercetoides , Hastichthys , Rhynchodercetis and Cyranichthys ), untoothed premaxillae (with Hastichthys and probably Rhynchodercetis ), and a single row of teeth on the maxillae (with Dercetis , Benthesikyme , Rhynchodercetis , Pelargorhynchus , Dercetoides and Hastichthys ). 相似文献
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The regulation and nitrate inhibition of nodule formation insoybean, Glycine max (L.) Merr., was further examined usingthe nodulation mutants of cv. Enrei. The non-nodulating mutantsEn115, Enl282, and En1314 produced extremely few markedly-curledroot hairs which were all devoid of infection threads, and invariablyfailed to initiate sub-epidermal cell divisions (SCDs) in theroot cortex. A considerable number of arrested SCDs was foundbefore nodule emergence in Enrei, but not in En6500 which hadsignificantly more SCDs that progressively increased at moreadvanced stages of nodule ontogeny. These observations indicatethat autoregulation acts by blocking the developmental stagebefore nodule emergence. In both Enrei and En65OO, the maturationof emerged nodules was restricted by a late-acting nodulationcontrol mechanism that is apparently unrelated to autoregulation.Reciprocal wedge-grafts of plants inoculated at sowing showedthat the control of the supernodulating phenotype resides inthe shoot, while the non-nodulating phenotype is strictly root-controlled.The nodulation phenotype of the current non-nodulating mutantsresults not from an alteration of the autoregulatory mechanism,but from mutation that exerts a root-localized effect that blocksSCDs which trigger the autoregulatory mechanism. Reciprocalgrafting experiments on Enrei and En6500 seedlings grown undervarious nitrate levels suggest that nitrate inhibition of nodulation,like autoregulation, is shoot-controlled. Since these two processesare invariably expressed together, they are probably causallyrelated, acting synergistically to regulate nodule formationin soybean. These results indicate that the regulation and nitrateinhibition of nodulation in the nodulation mutants of cv. Enreiare similar to those of cv. Bragg nodulation mutants. Key words: Autoregulation, nitrate-tolerant symbosis, non-nodulating mutants, soybean, supernodulating mutant 相似文献
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TIMOTHY COPPACK ILSE TINDEMANS MICHAEL CZISCH ANNEMIE VAN der LINDEN PETER BERTHOLD FRANCISCO PULIDO 《Global Change Biology》2008,14(11):2516-2522
Many organisms use day length as a cue for synchronizing their life cycles with seasonal changes in environmental productivity. Under rapid climate change, however, responses to day length may become maladaptive, and photo‐responsive organisms may only be able to evade increasingly unsuitable habitats if they can accommodate to a wide range of photoperiodic conditions. A previous experiment showed that the pied flycatcher, Ficedula hypoleuca, a Palaearctic‐Afrotropical migratory bird, would strongly advance the timing of spring migration and reproductive maturation if it shifted its wintering area from sub‐Saharan Africa to the Mediterranean region. However, it is unknown whether this marked response to latitudinal variation in photoperiodic conditions is continuous over the entire range of potential wintering areas, and if a shortening of migration distance would be an effective mechanism to adjust the timing of migration to rapidly changing climatic conditions. Here, we experimentally show that a moderate northward displacement of the pied flycatcher's current wintering grounds by 10° would result in a clear advancement of the termination of prenuptial moult and the initiation of spring migratory activity and gonadal growth. However, we found no further advancement under conditions simulating higher wintering latitudes, suggesting the existence of a critical photoperiodic threshold or a steep gradual response within a narrow geographical range between 10° and 20° northern latitude. Because habitat conditions in this area are deteriorating rapidly, the potential for pied flycatchers to adjust their life cycle to changing climatic conditions by shortening the migration distance may be limited in the future. 相似文献
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Testing the hypothesis of greater eurythermality in invasive than in native ladybird species: from physiological performance to life‐history strategies
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RODRIGO M. BARAHONA‐SEGOVIA AUDREY A. GREZ FRANCISCO BOZINOVIC 《Ecological Entomology》2016,41(2):182-191
1. Global warming and biological invasions are important threats to biodiversity. Nonetheless, there is little information on how these factors influence performance or life‐history traits of invasive and native species. 2. The effects of temperature on physiological and fitness traits of two invasive alien species (Harmonia axyridis and Hippodamia variegata) and one native species (Eriopis chilensis) of coccinellid were evaluated, testing a model of eurythermality. Eggs of all species were exposed to four temperature treatments (20, 24, 30 and 33 °C). In adult F2 we measured fecundity, locomotor performance, development time (total and per life stage), survival, and preferred body temperature in a thermal gradient. 3. It was found that H. axyridis had comparatively better performance at low temperatures (i.e. 20 °C), while the performance of H. variegata and E. chilensis did not change with temperature or was better at higher temperatures (30 °C). The standardised Levins index showed that all species are eurythermic. E. chilensis had a high niche overlap with the invasive alien ladybird species, rejecting the hypothesis of greater eurythermality of invasive species than native species. 4. Although there were differences in the temperature preferences and in the response of some physiological and life‐history traits of ladybirds to temperature, both the native and invasive alien species are eurythermic, contrary to the prediction. The better performance of H. axyridis at lower temperatures may result in displacement of its current distribution, and thus not all invasive species will respond favourably to global warming. 相似文献
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