The breeding biology of the Lapland Bunting Calcarius lapponicus in West Greenland during two contrasting years

The breeding biology of the Lapland Bunting was studied in an area of central West Greenland during two contrasting seasons. In the delayed thaw of 1984, male birds arrived on the breeding grounds at the same time as in the more typical spring of 1979, but females delayed arrival by two weeks in 1984 compared with 1979. Mean clutch size was depressed in the latter year and declined through the season; breeding productivity was correspondingly reduced in 1984 compared to 1979. Egg and juvenile predation rates were similar for the two years. Nestlings grew as rapidly in 1984 as in 1979 despite an apparent reduction in detected ground-dwelling invertebrates known to form an important constituent of the nestling diet. Because they bred later in 1984, females tended to commence moult later in 1984 than 1979 but completed moult by early September in both years. Males also commenced moult later but completed earlier in 1984.     

Genetic structure of a natural population of Dictyostelium discoideum, a cellular slime mould

Dictyostelium discoideum is a eukaryotic microbe feeding on soil bacteria. A first step towards describing the genetic structure of populations of this species was made by examining multiple isolates from a single locale. The isolates were grown clonally and their RFLP patterns compared, using a probe specific for a family of tRNA genes. Thirty-nine types were distinguished in 54 isolates. To determine if genetic exchange occurs among members of the population, an analysis of linkage disequilibrium was performed on the RFLP data. Little disequilibrium was found, implying gene flow in the population. In conflict with this result is the finding that no recombinant progeny were recovered from many attempted crosses between pairs of isolates. The tentative conclusion is that genetic exchange does not in fact occur, and that the observed shuffling of RFLP bands is caused by insertion and excision of transposons known to be associated with the tRNA genes of Dictyostelium.     

An Analysis of Seed Development in Pisum sativum: VI. ABSCISIC ACID ACCUMULATION

The abscisic acid (ABA) content of wrinkled (rr) pea seed tissueshas been quantified during development using multiple-ion-monitoringcombined gas chromatography-mass spectrometry and a deuteratedinternal standard. The level of ABA in the embryo generallyincreased with increasing cotyledon fresh weight while thatin the testa showed a distinct maximum at the time of maximumendosperm volume and the slowing in the growth of the testa.Pods contained relatively little ABA on a fresh weight basis.The total seed ABA content showed a biphasic distribution, thefirst maximum following the maximum growth rate of the testaand the second that of the embryo. The biphasic distributionof ABA in the pea seed was confirmed using a second pea genotype,near-isogenic to the first except for the r locus, and by theanalysis of individual seeds using a radioimmunoassay for ABA.The first maximum was composed mainly of a testa component andthe second mainly of an embryo component. When plants were grownin different environments, wrinkled seeds were found to containslightly more ABA than round (RR) but this was only significantlate in development. Immature seeds were capable of metabolizing17'-deoxy ABA to ABA, as determined by incorporation of either³H or ²H, and the metabolite was present mainly in the testa.The production of ABA in pea seeds is discussed in relationto the development of the different seed tissues. Key words: Abscisic acid, peas, seed development     

Class Seticoronaria and Phylogeny of the Phylum Priapulida

The Recent class Seticoronaria (Priapulida) as represented by the genus Maccabeus is compared with the newly described Priapulida from the Middle Cambrian Burgess Shale. It is concluded that the Seticoronaria retained such plesiomorphic characters of the Cambrian Priapulida as sedentary life and food trapping tentacles, whereas the other Recent class, the Priapulomorpha, is the derived, apomorphic branch of this small phylum. The bearing of this hypothesis on an eventual phylogenetic sequence of the Metazoa is discussed.     

Tumour Induction by Cell-free Extracts derived from Mice with Graft versus Host Disease

A SUBSTANTIAL proportion of inbred mice, in which a protracted form of the graft versus host reaction (GVHR) is induced, eventually develop lymphoreticular tumours¹. This experimental mouse model can be used to study the role of immune reactions in cancer induction. One possibility is that the immune disturbance activates a latent oncogenic virus. That such activation can occur has been demonstrated in C57BL mice². In this strain, with its low spontaneous leukaemic incidence, thymic lymphomas are induced by an irradiation-activated leukaemogenic virus. In man, there is suggestive evidence that Burkitt's lymphoma may be caused by a combination of virus infection and immunological stimulation³.