Experimental variations in sonic fragility of red cells

A magnetostriction oscillator has been used for determining the relative fragility of human red cells to 9000 cycle vibrations under some different environmental conditions. The destruction of the cells is a logarithmic function of time according to the equation See PDF for Equation. Hypertonic saline solution, saponin, preheating, alcohol, and ether in subhemolytic concentrations decrease the fragility of human red cells subjected to sonic oscillation. Hypotonic saline, preheating to 50°C. for 3 minutes, and hemolytic concentrations of ether increase the fragility of red cells to 9 kc. vibrations. Following preheating of the cells, and in the presence of saponin the destruction deviates slightly from a true logarithmic rate of hemolysis.     

Changes in the second stage juveniles of Globodeva rostochiensis prior to hatching in response to potato root diffusate

cAMP levels in eggs of G. rostochiensis and the diameter of the nucleolus of the nucleus within the dorsal pharyngeal gland cell of the second stage juvenile have been measured as indicators of the response of the nematode to the hatching stimulus in potato root diffusate. The nucleolus increased from 2.72 ± 0.103 μm for unhatched individuals to 3.28 ± 0.14 μm and 3.88 ± 0.15 μm after soaking eggs in potato root diffusate for 3 and 4 days respectively. Juveniles expressed from unstimulated eggs in water to potato root diffusate for 4–5 days showed a similar increase in size of the nucleolus to 3.94 ±0.15 μm but those released into water for this time had smaller nucleoli of 3.20 ± 0.98 μm. The change in diameter of the nucleolus is probably related to the accumulation of secretions in this gland cell before hatching. Preliminary results with dibutyryl analogues of CAMP and cGMP showed some inhibition of hatch in 10% potato root diffusate. Theophylline had a similar effect but NaF was dissimilar in that the effect of this inhibitor was not reversible. A standard radioimmunoassay showed that significant changes in cAMP levels occurred in the unhatched juveniles within cysts after treatment with potato root diffusate for 2.5 or 8 h compared with values for cysts kept in water. This change occurs before other known responses of the juveniles to potato root diffusate and it defines the period of interest for future work on the initial action of hatching factor.     

Reproductive strategies of the exotic Pharaoh's ant,Monomorium pharaonis (L.) (Hymenoptera: Formicidae) in Brazil

Summary

The production of sexual forms in laboratory colonies of Monomorium pharaonis was followed over a 19-month period. Cycles of production occurred at intervals of 4 months, with male investment inversely proportional to the number of gynes produced. There was a tendency for colonies to produce only males or females as total sexual production increased. Sperm counts of known-aged males suggest that they mate twice, in contrast to European populations. Sex-ratio was heavily female-biased, contrary to predictions for polygynous ant species.     

Population dynamics of Mallophaga and Acari on reed buntings occupying a communal winter roost

Abstract. 1. Mallophaga and Acari were collected from 324 reed buntings (Emberiza schoeniclus (Linnaeus)) captured in a communal winter roost between October 1980 and March 1981.
2. Frequency distributions of one of the mallophagan species, (Philopterus docophorulus (Zlotorzycka)), of the aggregated Mallophaga, and of the aggregated Acari, conform well with a negative binomial model. The remaining three mallophagan species collected were not present in sufficient numbers to describe a frequency distribution.
3. The ratio of nymphs to adults in the mallophagan community increased significantly in the second half of the roosting period (January–March) indicating the onset of increased reproductive activity.     

Variations in the configuration of algae in subtidal forests: Implications for invertebrate assemblages

Abstract The ecology of Australia's most extensive canopy‐forming alga, Ecklonia radiata, is often studied with little regard as to whether it occurs in monospecific stands or as part of a mixed assemblage of canopy‐forming algae. We tested the hypothesis that E. radiata does not primarily occur as monospecific stands, rather it occurs more often in stands of mixed algae. At a 1‐m² scale we recognized three main configurations within forests of algae (hereafter called stands): E. radiata that occurs as (i) monospecific stands; (ii) clumps (four or more individuals together) surrounded by species of Fucales; or (iii) individual plants (or clusters of fewer than three plants) interspersed among species of Fucales. All three types of stand occurred in similar proportions (percentage cover) across two regions of Australia's southern coastline (Western and South Australia). We also tested the hypothesis that these three types of stands (identified at 1 m²) contain different assemblages of invertebrates associated with the holdfast of E. radiata. Assemblages of invertebrates varied between monospecific and interspersed stands, but not between monospecific and clumped stands. These results suggest that variation in the configuration of subtidal algae (stands measured at a 1‐m² scale) has the potential to influence the composition and abundance of associated biota. We suggest that although studies in stands of monospecific E. radiata may provide useful information for the majority of forests containing E. radiata (monospecific and clumped stands made up 65% of forests sampled), caution must be used when extrapolating to stands of mixed, interspersed algae (>31% of forests sampled).     

Places Of Transformation: Building Monuments From Water And Stone In The Neolithic Of The Irish Sea

Using the Irish Sea area as a case-study, we argue that both sites and landscapes can be understood as containing a series of components procured from the landscape and from human, animal, and object bodies. These components were organized in a way that commented on and related to specific cultural relationships between these different locations and through the substances found within them. This idea is explored by examining Neolithic monuments, material culture, and natural materials in southwest Wales, northwest Wales, the Isle of Man, and southwest Scotland. We trace some metaphorical schemes which were integral to Neolithic activity in this part of the Irish Sea. In particular, we highlight the metaphorical connections between water and stone in places associated with transformation, particularly the repeated transformation of human bodies. We suggest that the series of associations present in the Neolithic were not invested with a uniform meaning but, instead, were polyvalent, subject to conflicting interpretations, contextually specific and variable through both space and time. The relationship between these elements was therefore dependent on the contexts of their association. Nevertheless, the association of water and stone can be found repeatedly throughout the Neolithic world and may have been the medium of a powerful trope within broader conceptions of the world. This article is intended as a preliminary consideration of these issues (particularly the links between stone, mountains, water, quartz, shell, and human remains) and is offered as a thinking-point for ongoing research in this area.