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141.
Abstract: This paper describes the diversity, taphonomy and palaeoecology of angiosperm leaves that dominate a palaeoflora of Cretaceous (Cenomanian–Coniacian) age from the Mata Amarilla Formation in the Austral Basin, south-west Patagonia, Argentina. Twelve morphotypes of angiosperm leaves are recognized based on foliar morphotype analysis of more than 500 specimens. These were divided into six morphological groups based on major architectural patterns. The relative dominance of these morphotypes, mode of preservation and relationship with sedimentary facies were evaluated from two levels within the formation. This analysis identified two different plant palaeocommunities. The lower, María Elena, level (MEL) was deposited in a marine coastal area on a subaerial delta plain; the dominant angiosperm morphotypes preserved in it are group 1 (MA100) and group 2 (MA101, 102); morphotypes MA109 and 110 are scarce but exclusive to this level. The upper, Mata Amarilla, level (MAL), accumulated inland in flood-plain environments; the most abundant angiosperm morphotypes are groups 3 (MA103–105), 4 (MA106) and 1 (MA100); morphotypes MA103–105 and 108 are exclusive to this level. Comparisons with other floras of similar age from Antarctica, Australia and New Zealand indicate that the Mata Amarilla flora has a slightly higher morphological diversity of angiosperm leaves, providing the first evidence for an angiosperm-dominated early Late Cretaceous macroflora in south-west Gondwana. 相似文献
142.
CHRISTOPH ANDREAS LEHMEIER FERNANDO ALFREDO LATTANZI RUDI SCHÄUFELE & HANS SCHNYDER 《Plant, cell & environment》2010,33(1):76-87
Plant respiration draws on substrate pools of different functional/biochemical identity. Little is known about the effect of nitrogen deficiency on those pools' sizes, half-lives and relative contribution to respiration, and consequently, of carbon residence time in respiratory metabolism. Here we studied how nitrogen fertilization affects the respiratory carbon supply system of shoots and roots of Lolium perenne , a perennial grass. Plants grown at two nitrogen supply levels in continuous light were labelled with 13 CO2 /12 CO2 for intervals ranging from 1 h to 1 month. The rate and isotopic composition of shoot, root and plant respiration were measured, and the time-courses of tracer incorporation into respired CO2 were analysed by compartmental modelling. Nitrogen deficiency reduced specific respiration rate by 30%, but increased the size of the respiratory supply system by 30%. In consequence, mean residence time of respiratory carbon increased with nitrogen deficiency (4.6 d at high nitrogen and 9.2 d at low nitrogen supply). To a large extent, this was due to a greater involvement of stores with a long half-life in respiratory carbon metabolism of nitrogen-deficient plants. At both nitrogen supply levels, stores supplying root respiration were primarily located in the shoot, probably in the form of fructans. 相似文献
143.
144.
MATTIA BRAMBILLA SEVERINO VITULANO ANDREA FERRI FERNANDO SPINA ELENA FABBRI ETTORE RANDI 《Ibis》2012,154(3):616-620
Genetic markers are often used to trace the geographical origin of migrating birds. Such an approach has been used to attribute individuals of a given species to a given population, but it could also be applied to cryptic species, which are not fully diagnosable on the basis of their morphological appearance alone, despite often being genetically distinct. We sampled migrants of the Sylvia cantillans complex, which include cryptic taxa not readily identified on the basis of their appearance, at spring stopover sites in the central Mediterranean. We identified these individuals taxonomically using mitochondrial DNA sequence data. Molecular analyses enabled us to establish that four different taxa/clades of the Sylvia cantillans complex (two S. c. cantillans clades, S. c. albistriata and S. subalpina) migrate through the central‐western Mediterranean from Africa. 相似文献
145.
146.
ISABEL FERNANDES CLÁUDIA PASCOAL HELENA GUIMARÃES RUTE PINTO INÊS SOUSA FERNANDA CÁSSIO 《Freshwater Biology》2012,57(11):2306-2317
1. We investigated the effects of riparian plant diversity (species number and identity) and temperature on microbially mediated leaf decomposition by assessing fungal biodiversity, fungal reproduction and leaf mass loss. 2. Leaves of five riparian plant species were first immersed in a stream to allow microbial colonisation and were then exposed, alone or in all possible combinations, at 16 or 24 °C in laboratory microcosms. 3. Fungal biodiversity was reduced by temperature but was not affected by litter diversity. Temperature altered fungal community composition with species of warmer climate, such as Lunulospora curvula, becoming dominant. 4. Fungal reproduction was affected by litter diversity, but not by temperature. Fungal reproduction in leaf mixtures did not differ or was lower than that expected from the weighted sum of fungal sporulation on individual leaf species. At the higher temperature, the negative effect of litter diversity on fungal reproduction decreased with the number of leaf species. 5. Leaf mass loss was affected by the identity of leaf mixtures (i.e. litter quality), but not by leaf species number. This was mainly explained by the negative correlation between leaf decomposition and initial lignin concentration of leaves. 6. At 24 °C, the negative effects of lignin on microbially mediated leaf decomposition diminished, suggesting that higher temperatures may weaken the effects of litter quality on plant litter decomposition in streams. 7. The reduction in the negative effects of lignin at the higher temperature resulted in an increased microbially mediated litter decomposition, which may favour invertebrate‐mediated litter decomposition leading to a depletion of litter stocks in streams. 相似文献
147.
BRUNO DA ROCHA-AZEVEDO MELISSA JAMERSON GUY A. CABRAL FERNANDO C. SILVA-FILHO FRANCINE MARCIANO-CABRAL 《The Journal of eukaryotic microbiology》2009,56(3):270-278
ABSTRACT. Acanthamoeba are free-living amoebae that are dispersed in most environments. Occasionally, Acanthamoeba cause serious human infections, such as keratitis and encephalitis. During the infection process, amoebic adhesion to, and degradation of, host cells and their extracellular matrix (ECM) appear to be important requirements. We examined the interaction of Acanthamoeba with the ECM, and related this event to host cell destruction and tissue invasion. Pathogenic Acanthamoeba culbertsoni differentially attached on the ECM glycoproteins laminin-1, collagen-I, and fibronectin, as compared with non-pathogenic Acanthamoeba astronyxis . Binding to collagen-I and laminin-1 induced A. culbertsoni to become flattened and elongated. Because attachment on laminin-1 was higher in A. culbertsoni , laminin-1 was chosen for further analysis. A 55-kDa laminin-binding protein was identified in pathogenic amoebae, but it was not found in non-pathogenic amoebae. No differential cytotoxicity against distinct cell types was observed between A. culbertsoni incubated with or without ECM. On the other hand, binding on collagen-I or matrigel scaffolds induced a differential effect where A. culbertsoni invaded collagen-I matrices more rapidly. These results indicate that ECM recognition, as an antecedent to tissue invasion, may be a trait characteristic of pathogenic Acanthamoeba . 相似文献
148.
CARMEN MANSILLA FLORA SÁNCHEZ HAL S. PADGETT † GREGORY P. POGUE ‡ FERNANDO PONZ 《Molecular Plant Pathology》2009,10(1):59-68
Oilseed rape mosaic virus (ORMV) is a tobamovirus taxonomically distinct from the type member of the genus, Tobacco mosaic virus (TMV). Both viruses display a specific host range, although they share certain hosts, such as Arabidopsis thaliana , Nicotiana benthamiana and N. tabacum , on which they induce different symptoms. Using a gain-of-symptom approach, we generated chimeric viruses, starting from a TMV infectious clone, over which different regions of ORMV were exchanged with their corresponding regions in the TMV genome. This approach allowed the association of pathogenicity determinants to certain genes within the ORMV genome. A general trend was observed associating the viral origin of the RNA-dependent RNA-polymerase ( RdRp ) gene and the gain of symptoms. In A. thaliana and N. benthamiana , chimeric viruses were unable to reproduce the symptoms induced by the parental viruses, leading to disease states which could be described as intermediate, and variable in some cases. In contrast, a hypersensitive reaction caused by both of these viruses on N -gene-bearing tobaccos could be found in resistance reactions to all chimeric viruses, suggesting that the avirulence determinant maps similarly in both viruses. A systemic necrotic spotting typical of non- N -gene tobaccos infected with ORMV was associated with the polymerase domain of RdRp . To our knowledge, this is the first time that this controversial portion of the tobamovirus genome has been identified directly as a pathogenicity determinant. None of the reactions of the chimeric viruses could be correlated with increases or decreases in virus titres in the infections. 相似文献
149.
LEILA M. ODA CELUTA S. ALVIANO FERNANDO C. SILVA FILHO JAYME ANGLUSTER ISAAC ROITMAN WANDERLEY DE SOUZA 《The Journal of eukaryotic microbiology》1984,31(1):131-134
The surface anionic groups of symbiote-bearing and symbiote-free strains of Crithidia deanei were compared by determining cellular electrophoretic mobility, by ultrastructural cytochemistry, and by identification of sialic acids by thin-layer and gasliquid chromatography. Symbiote-free Crithidia deanei has a highly negative surface charge (-0.9984 μm?s-1? V-1? cm), which is slightly reduced (-0.8527 μm?s-1? V-1? cm) by the presence of the endosymbiote. Treatment of both strains of C. deanei with neuraminidase decreased significantly the electrophoretic mobility of cells toward the cathodic pole, indicating the existence of exposed sialic acid residues responsible for the negative charge on the protozoan cell surface. Thin-layer and gas-liquid chromatography showed that N-glycolyl- and N-acetylneuraminic acids were present in both strains of C. deanei. 相似文献
150.
Studies on plant cuticle 总被引:2,自引:0,他引:2
A. M. S. SILVA FERNANDES 《The Annals of applied biology》1965,56(2):305-313