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941.
Alexander V. Kirdyanov Eugene A. Vaganov Malcolm K. Hughes 《Trees - Structure and Function》2007,21(1):37-44
We propose a technique for separating the climatic signal which is contained in two tree-ring parameters widely used in dendroclimatology.
The method is based on the removal of the relationship between tree-ring width (TRW) and maximum latewood density (MXD) observed
for narrow tree rings from high latitudes. The new technique is tested on data from three larch stands located along the northern
timberline in Eurasia. Correlations were calculated between the temperatures of pentads (five consecutive days), TRW chronologies
and MXD chronologies calculated according to the standard and proposed methods. The analysis confirms the great importance
of summer temperature for tree radial growth and tree-ring formation. TRW is positively correlated with the temperature of
four to eight pentads (depending on the region) at the beginning of the growth season, but MXD as obtained by the standard
technique is correlated with temperature over a much longer period. For maximum density series from which the relationship
between MXD and TRW has been removed (MXD′), there is a clear correlation with temperatures in the second part of the growing
season. These results are consistent with the known dynamics of tree-ring growth in high latitudes and mechanisms of tree-ring
formation. 相似文献
942.
943.
944.
945.
V. Selvakumar P. R. Anbudurai T. Balakumar 《In vitro cellular & developmental biology. Plant》2001,37(2):280-284
Summary A protocol for rapid in vitro propagation using nodal explants obtained from 2-yr-old, field-grown medicinal plants of Plumbago zeylanica L. belonging to the family Plumbaginaceae is described. High frequency bud break and fast development of shoots were induced
on Murashige and Skoog's basal medium supplemented with 27.2 μM adenine sulfate +2.46 μM indole-3-butyric acid (IBA). Induction of rooting was achieved by transferring the shoots to the same basal medium containing
4.92 μM IBA. Using our protocol from one twig of P. zeylanica (eight responsive nodes per explant shoot) within a period of 5 mo., eight plantlets could be raised. After a hardening period
of 4 wk, there was a 90% transplantation success in the field compared to the 60–65% survival of plantlets recorded in the
experiments of previous workers. The plantlets derived through in vitro propagation mimic the growth and morphological characteristics of the donor plants. 相似文献
946.
947.
948.
A mutant strain of Penicillium citrinum grown in a chemically-defined production medium, yielded 145 mg compactin l–1. The medium also facilitated spectrophotometric analysis of compactin. Addition of KH2PO4in the production medium did not increase the compactin production, while addition of a surfactant, Tween 80, increased compactin to 175 mg l–1. Inoculation with 107 spores ml–1 and initial pH of 6.5–7 were the most suitable for compactin production. 相似文献
949.
950.
M R Harris C S Kindle A F Abruzzini C W Pierce S E Cullen 《Journal of immunology (Baltimore, Md. : 1950)》1984,133(3):1202-1208
We examined the antigen-presenting capacity of BCL1 tumor cells, which are capable of differentiating in vitro with respect to immunoglobulin synthesis/secretion under the influence of LPS. In vivo passaged BCL1 cells depleted of host cell contamination either by positive selection employing panning with anti-lambda reagents, or by elimination of latex-ingesting adherent cells, are capable of MHC-restricted antigen presentation to a GAT-immune T cell line. The BCL1 cells act as antigen-presenting cells when freshly explanted, but gradual loss of this function occurs, and cells cultured for 3.5 days cannot present antigen unless LPS is included during the culture period. BCL1 cells are equivalently Ia+ after the culture period with or without LPS stimulation. Other B cell lines capable of antigen presentation appear to express this trait constitutively, and the in vivo passaged BCL1 line is therefore unique among B cell lines in having antigen-presenting cell function that can be modulated. The data suggest that freshly explanted or LPS-cultured BCL1 cells are heterogeneous with respect to antigen-presenting capacity, and the basis for this heterogeneity is being sought. BCL1 offers an opportunity to study requirements for antigen presentation by B cells. 相似文献