全文获取类型
收费全文 | 347390篇 |
免费 | 34184篇 |
国内免费 | 262篇 |
出版年
2018年 | 4198篇 |
2017年 | 3885篇 |
2016年 | 5413篇 |
2015年 | 7336篇 |
2014年 | 7996篇 |
2013年 | 11520篇 |
2012年 | 12768篇 |
2011年 | 12404篇 |
2010年 | 8204篇 |
2009年 | 7098篇 |
2008年 | 10806篇 |
2007年 | 10703篇 |
2006年 | 10347篇 |
2005年 | 9604篇 |
2004年 | 9502篇 |
2003年 | 8974篇 |
2002年 | 8585篇 |
2001年 | 13378篇 |
2000年 | 13324篇 |
1999年 | 10511篇 |
1998年 | 3934篇 |
1997年 | 4012篇 |
1996年 | 3962篇 |
1995年 | 3529篇 |
1994年 | 3479篇 |
1993年 | 3426篇 |
1992年 | 8621篇 |
1991年 | 8548篇 |
1990年 | 8208篇 |
1989年 | 8220篇 |
1988年 | 7465篇 |
1987年 | 7249篇 |
1986年 | 6608篇 |
1985年 | 6863篇 |
1984年 | 5690篇 |
1983年 | 4838篇 |
1982年 | 3771篇 |
1981年 | 3523篇 |
1980年 | 3294篇 |
1979年 | 5540篇 |
1978年 | 4255篇 |
1977年 | 4170篇 |
1976年 | 3834篇 |
1975年 | 4327篇 |
1974年 | 4749篇 |
1973年 | 4645篇 |
1972年 | 4182篇 |
1971年 | 3879篇 |
1970年 | 3560篇 |
1969年 | 3553篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
901.
902.
903.
904.
905.
F. Vierhapper 《Plant Systematics and Evolution》1921,70(9-12):261-293
906.
907.
908.
Independent genes coding for three acidic proteins of the large ribosomal subunit from Saccharomyces cerevisiae 总被引:11,自引:0,他引:11
M Remacha M T Sáenz-Robles M D Vilella J P Ballesta 《The Journal of biological chemistry》1988,263(19):9094-9101
The yeast ribosome contains three acidic proteins, L44, L44', and L45, closely related from a structural point of view, that seem to play a functional role similar to that of proteins L7 and L12 in the bacterial ribosome. By screening a cDNA bank in lambda gt11 with specific polyclonal and monoclonal antibodies, recombinant phages expressing each one of the acidic proteins have been cloned. A unique copy of each gene is detected using the phage cDNA inserts as probes in nitrocellulose blots of yeast DNA digested with different restriction enzymes. The inserts were subcloned in the plasmid pUC19, and their physical maps and nucleotide sequences were determined. By using the cDNA inserts as probes in genomic DNA banks, DNA fragments carrying the acidic protein genes have been cloned, characterized, and sequenced. The results conclusively show that the three yeast acidic proteins are coded by independent genes and are not the result of a post-translational modification of the product of a unique gene, as in bacteria. Like most ribosomal protein genes, the gene for protein L44' has an intron and two upstream stimulatory boxes (UASrpg) fitting closely to the consensus sequence. The genes coding for proteins L44 and L45 lack introns and seem also exceptional in other characteristics of their sequences. Proteins L44 and L45 have amino acid sequences with about 80% similarity. Protein L44' is only 63% similar to the other two polypeptides. The three proteins have highly conserved carboxyl termini comprising the last 30 amino acids, and the first 10 amino acids of L44 and L45 are identical. The results cast doubts about the possibility of a similar role for the different acidic ribosomal proteins. 相似文献
909.
Nonlymphoid, stromal cells in the mouse thymus are believed to be important in T cell maturation and have been proposed to play a central role in the acquisition of major histocompatibility complex (MHC) restriction and self-tolerance by maturing thymocytes. Both cortical and medullary epithelial cells in the thymus express high levels of class II (A) major histocompatibility antigens (MHC Ags). We show here that a specific subset of these A– epithelial cells express a transformation-associated antigen (6C3Ag) found previously on the surfaces of Abelson murine leukemia virus-transformed pre-B cells and on those bone marrow-derived stromal cell clones which support normal and preneoplastic pre-B cell proliferation. Among solid lymphoid organs, only the thymus contains 6C3Ag1 cells and within the thymus, this antigen is found exclusively on A– epithelial cells in cortical regions. It is striking that the expression of the 6C3Ag on thymic epithelium is developmentally regulated, suggesting a role for this lymphostromal antigen in the maturation of the thymic microenvironment. 相似文献
910.