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21.
We previously identified pituitary tumor-derived fibroblast growth factor receptor 4 (ptd-FGFR4), an alternatively transcribed N-terminally truncated cytoplasmic receptor isoform. Unlike wild-type FGFR4, ptd-FGFR4 facilitates cell transformation and results in pituitary tumor formation in transgenic mice. To investigate differences in the tumorigenic properties of FGFR4 and ptd-FGFR4, we examined their abilities to modulate cell adhesiveness. Introduction of ptd-FGFR4 into GH4 pituitary cells or NIH 3T3 fibroblasts resulted in significant reduction in cell adhesion to a collagen IV matrix compared with FGFR4- or empty vector-transfected cells. This adhesive difference was evident in the absence or presence of FGF stimulation. Furthermore, treatment with beta1-integrin neutralizing antibody markedly reduced adhesiveness in FGFR4-transfected cells but had little effect on the depressed adhesiveness of ptd-FGFR4-transfected cells. Unlike wild-type FGFR4, ptd-FGFR4 does not associate with neural cell-adhesion molecule (NCAM). Cells expressing FGFR4 demonstrate membranous N-cadherin with a noninvasive growth pattern identical to control GH4 cells when injected into immunodeficient mice. In contrast, ptd-FGFR4-expressing cells develop invasive tumors in vivo with marked loss of N-cadherin that localizes to the cytoplasm. Consistent with these changes, beta-catenin expression was diminished and its interaction with N-cadherin was disrupted in the presence of ptd-FGFR4, but both were intact in the presence of wild-type FGFR4. These data highlight the importance of membrane-anchored FGFR4 in assembling a multiprotein FGFR4 complex with NCAM and N-cadherin playing pivotal functions in maintaining normal cell adhesion. Disruption of distinct NCAM/N-cadherin proadhesive complexes by a tumor-derived FGFR4 isoform provides a novel mechanism beyond ligand independence that explains the pathobiology of proliferative and infiltrative but nonmetastatic neoplasms. 相似文献
22.
Ahmed A. El-Sherif 《Inorganica chimica acta》2009,362(14):4991-549
We report the synthesis of the hydrazone ligands, 1-(phenyl-hydrazono)-propan-2-one (PHP), 1-(p-tolyl-hydrazono)-propan-2-one (THP), 1-[(4-chloro-hydrazono)]-propan-2-one (CHP), and their Ni(II) and Cu(II) metal complexes. The structure of the ligands and their complexes were investigated using elemental analysis, magnetic susceptibility, molar conductance and spectral (IR, UV, and EPR) measurements. IR spectra indicate that the free ligands exist in the hydrazo-ketone rather than azo-enol form in the solid state. Also, the hydrazo-NH exists as hydrogen bonded to the keto-oxygen either as intra or as intermolecular hydrogen bonding. In all the studied complexes, all ligands behave as a neutral bidentate ligands with coordination involving the hydrazone-nitrogen and the keto-oxygen atoms. The magnetic and spectral data indicate a square planar geometry for Cu2+ complexes and an octahedral geometry for Ni2+ complexes. The ligands and their metal chelates have been screened for their antimicrobial activities using the disc diffusion method against the selected bacteria and fungi. They were found to be more active against Gram-positive than Gram-negative bacteria. It may be concluded that the antimicrobial activity of the compounds is related to cell wall structure of bacteria.Protonation constant of (PHP) ligand and stability constants of its Cu2+ and Ni2+ complexes were determined by potentiometric titration method in aqueous solution at ionic strength of 0.1 M sodium nitrate. It has been observed that the hydrazone ligand (PHP) titrated here has one protonation constant. The divalent metal ions Cu2+ and Ni2+ form with (PHP) 1:1 and 1:2 complexes. The insolubility of (THP) and (CHP) ligands in aqueous medium does not permit the determination of their protonation constants and formation constants of the corresponding complexes in aqueous solution. 相似文献
23.
An efficient improved procedure for the synthesis of beta-acetamido ketones has been developed by a heteropoly acid (HPA) catalyzed three-component coupling protocol. The present methodology offers several advantages such as excellent yields, simple procedure, short reaction times, and mild conditions. 相似文献
24.
25.
12-Tungstophosphoric acid (PW) supported on different metal oxides (SiO2, γ-Al2O3, KSF, K10) and activated carbon were prepared by impregnation method and their catalytic performances were evaluated in three component condensation of benzaldehyde, ethyl acetoacetate and ammonium acetate to afford corresponding 1,4-dihydropyridine. A high catalytic activity was found over silica supported PW. Effect of PW loading, catalyst loading and solvent was studied to introduce the best reaction condition. Based on the above experimental finding, catalytic performances was optimized with a loading of 40% PW onto SiO2 (0.2 g) under solvent-free condition. The characterization data derived from FT-IR, XRD, and TGA-DSC techniques reveal that the PW on silica support exists in Keggin structure. In addition, acidity measurements were performed by potentiometric titration with n-butylamine. The activity of the catalysts is strongly dependent on their acidic characteristic which, in turn, depended on PW loading. Finally, a series of 4-aryl, N-alkyl, and N-aryl substituted 1,4-dihydropyridines have been synthesized in high to excellent yield in short reaction times. PW/SiO2 was found to be reusable and a considerable catalytic activity still could be achieved after fourth run. 相似文献
26.
27.
J. Baumgartner I. E. Ezzat Z. Končeková 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1986,72(2):264-268
Summary Two experiments were conducted to verify the real possibility for use of the genetic transformation technique as described by Pandey and Patchel in chickens in commercial poultry breeding. Multiple recessive and multiple dominant marker stocks were employed, as well as a tester and a donor line. Recipient tester females were first inseminated with dominant donor semen which was irradiated with doses of 60Co gamma irradiation ranging from 100 to 800 Gy (control group) and 24 h later were reinseminated with unirradiated, normal semen of the recipient strain (experimental group). One genetic transformed chicken was found in the first experiment; no genetic transformed event occurred in the second experiment but one embryo was present in the 600 Gy irradiated group with parthenogenetic development capable of giving a live chick. One hundred Gy was observed not to be enough to destroy completely sperm fertilizing ability. An increased frequency of parthenogenetic development was found in all groups after insemination with irradiated semen. There were 11 individuals with developmental abnormalities from the total of 1264 analysed embryos which died after the 18th day of incubation. We concluded that egg transformation is a rare event in domestic fowl and further research for use of this technique in commercial poultry breeding is needed. 相似文献
28.
Erythropoietin protects cardiac myocytes from hypoxia-induced apoptosis through an Akt-dependent pathway 总被引:41,自引:0,他引:41
Tramontano AF Muniyappa R Black AD Blendea MC Cohen I Deng L Sowers JR Cutaia MV El-Sherif N 《Biochemical and biophysical research communications》2003,308(4):990-994
Apoptosis is a contributing cause of myocyte loss in ischemic heart disease. Recent work has shown that erythropoietin (EPO) offers protection against apoptosis in a wide variety of tissues. We demonstrate that the erythropoietin receptor (EPOR) is expressed in the neonatal rat ventricular myocyte (NRVM). Exposure of NRVMs to hypoxia, with recombinant human EPO, significantly decreased apoptosis as measured by TUNEL, flow cytometry, and caspase 3/7 like activity when compared to hypoxia treatment alone. EPO administered at the initiation of coronary artery occlusion in the rat significantly decreased apoptosis in the myocardial ischemic region. In the NRVM, EPO increased the activity of Akt. The anti-apoptotic effect of EPO was abrogated by co-treatment with LY294002, a specific blocker of phosphatidylinositol 3-kinase (PI3-K). Our study demonstrates that EPO inhibits apoptosis in the NRVM exposed to hypoxia, through an Akt-dependent pathway. EPO also inhibits apoptosis in the in vivo rat model of myocardial ischemia. 相似文献
29.
In the short-germ beetle Tribolium castaneum, waves of pair-rule gene expression propagate from the posterior end of the embryo towards the anterior and eventually freeze into stable stripes, partitioning the anterior-posterior axis into segments. Similar waves in vertebrates are assumed to arise due to the modulation of a molecular clock by a posterior-to-anterior frequency gradient. However, neither a molecular candidate nor a functional role has been identified to date for such a frequency gradient, either in vertebrates or elsewhere. Here we provide evidence that the posterior gradient of Tc-caudal expression regulates the oscillation frequency of pair-rule gene expression in Tribolium. We show this by analyzing the spatiotemporal dynamics of Tc-even-skipped expression in strong and mild knockdown of Tc-caudal, and by correlating the extension, level and slope of the Tc-caudal expression gradient to the spatiotemporal dynamics of Tc-even-skipped expression in wild type as well as in different RNAi knockdowns of Tc-caudal regulators. Further, we show that besides its absolute importance for stripe generation in the static phase of the Tribolium blastoderm, a frequency gradient might serve as a buffer against noise during axis elongation phase in Tribolium as well as vertebrates. Our results highlight the role of frequency gradients in pattern formation. 相似文献
30.
Morteza Moghaddasian Hamidreza Arab Ezzat Dadkhah Hamidreza Boostani Azam Rezaei Babak Mohammad Reza Abbaszadegan 《Gene》2014