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31.
Menotta M Amicucci A Basili G Rivero F Polidori E Sisti D Stocchi V 《Protoplasma》2007,231(3-4):227-237
Summary. The small GTPase CDC42 is ubiquitously expressed in eukaryotes, where it participates in the regulation of the cytoskeleton
and a wide range of cellular processes, including cytokinesis, gene expression, cell cycle progression, apoptosis, and tumorigenesis.
As very little is known on the molecular level about mycorrhizal morphogenesis and development and these events depend on
a tightly regulated reorganisation of the cytoskeleton network in filamentous fungi, we focused on the molecular characterisation
of the cdc42 gene in Tuber borchii Vittad., an ascomycetous hypogeous fungus forming ectomycorrhizae. The entire gene was isolated from a T. borchii cDNA library and Southern blot analyses showed that only one copy of cdc42 is present in the T. borchii genome. The predicted amino acid sequence is very similar to those of other known small GTPases and the similar domain structures
suggest a similar function. Real-time PCR analyses revealed an increased expression of Tbcdc42 during the phase preparative to the instauration of symbiosis, in particular after stimulation with root exudate extracts.
Immunolocalisation experiments revealed an accumulation of CDC42 in the apical tips of the growing hyphae. When a constitutively
active Tbcdc42 mutant was expressed in Saccharomyces cerevisiae, morphological changes typical of pseudohyphal growth were observed. Our results suggest a fundamental role of CDC42 in cell
polarity development in T. borchii.
Correspondence and reprints: Istituto di Chimica Biologica “G. Fornaini”, Università degli Studi di Urbino, Via Saffi 2, 61029
Urbino, Italy. 相似文献
32.
33.
Jin J Wang Y Wang F Kerns JK Vinader VM Hancock AP Lindon MJ Stevenson GI Morrow DM Rao P Nguyen C Barrett VJ Browning C Hartmann G Andrew DP Sarau HM Foley JJ Jurewicz AJ Fornwald JA Harker AJ Moore ML Rivero RA Belmonte KE Connor HE 《Bioorganic & medicinal chemistry letters》2007,17(6):1722-1725
High-throughput screening of the corporate compound collection led to the discovery of a novel series of N-substituted-5-aryl-oxazolidinones as potent human CCR8 antagonists. The synthesis, structure-activity relationships, and optimization of the series that led to the identification of SB-649701 (1a), are described. 相似文献
34.
Yamile Gonzlez Aparecida S. Tanaka Izaura Y. Hirata Maday Alonso del Rivero Maria L.V. Oliva Mariana S. Araujo Maria A. Chvez 《Comparative biochemistry and physiology. Part A, Molecular & integrative physiology》2007,146(4):506
Human neutrophil elastase inhibition was detected in a crude extract of the marine snail Cenchritis muricatus (Gastropoda, Mollusca). This inhibitory activity remained after heating this extract at 60 °C for 30 min. From this extract, three human neutrophil elastase inhibitors (designated CmPI–I, CmPI–II and CmPI–III) were purified by affinity and reversed-phase chromatographies. Homogeneity of CmPI–I and CmPI–II was confirmed, while CmPI–III showed a single peak in reversed-phase chromatography, but heterogeneity in SDS-PAGE with preliminary molecular masses in the range of 18.4 to 22.0 kDa. In contrast, MALDI-TOF mass spectrometry of CmPI–I and CmPI–II showed that these inhibitors are molecules of low molecular mass, 5576 and 5469 Da, respectively. N-terminal amino acid sequences of CmPI–I (6 amino acids) and CmPI–II (20 amino acids) were determined. Homology to Kazal-type protease inhibitors was preliminarily detected for CmPI–II. Both inhibitors, CmPI–I and CmPI–II are able to inhibit human neutrophil elastase strongly, with equilibrium dissociation constant (Ki) values of 54.2 and 1.6 nM, respectively. In addition, trypsin and pancreatic elastase were also inhibited, but not plasma kallikrein or thrombin. CmPI–I and CmPI–II are the first human neutrophil elastase inhibitors described in a mollusk. 相似文献
35.
Subhanjan Mondal Dhamodharan Neelamegan Francisco Rivero Angelika A Noegel 《BMC cell biology》2007,8(1):23
Background
Rho subfamily GTPases are implicated in a large number of actin-related processes. They shuttle from an inactive GDP-bound form to an active GTP-bound form. This reaction is catalysed by Guanine nucleotide exchange factor (GEFs). GTPase activating proteins (GAPs) help the GTPase return to the inactive GDP-bound form. The social amoeba Dictyostelium discoideum lacks a Rho or Cdc42 ortholog but has several Rac related GTPases. Compared to our understanding of the downstream effects of Racs our understanding of upstream mechanisms that activate Rac GTPases is relatively poor. 相似文献36.
37.
Janine M Ramsey A Townsend Peterson Oscar Carmona-Castro David A Moo-Llanes Yoshinori Nakazawa Morgan Butrick Ezequiel Tun-Ku Keynes de la Cruz-Félix Carlos N Ibarra-Cerde?a 《Memórias do Instituto Oswaldo Cruz》2015,110(3):339-352
Chagas disease is one of the most important yet neglected parasitic diseases in
Mexico and is transmitted by Triatominae. Nineteen of the 31 Mexican triatomine
species have been consistently found to invade human houses and all have been found
to be naturally infected with Trypanosoma cruzi. The present paper
aims to produce a state-of-knowledge atlas of Mexican triatomines and analyse their
geographic associations with T. cruzi, human demographics and
landscape modification. Ecological niche models (ENMs) were constructed for the 19
species with more than 10 records in North America, as well as for T.
cruzi. The 2010 Mexican national census and the 2007 National Forestry
Inventory were used to analyse overlap patterns with ENMs. Niche breadth was greatest
in species from the semiarid Nearctic Region, whereas species richness was associated
with topographic heterogeneity in the Neotropical Region, particularly along the
Pacific Coast. Three species, Triatoma longipennis, Triatoma
mexicana and Triatoma barberi, overlapped with the
greatest numbers of human communities, but these communities had the lowest
rural/urban population ratios. Triatomine vectors have urbanised in most regions,
demonstrating a high tolerance to human-modified habitats and broadened historical
ranges, exposing more than 88% of the Mexican population and leaving few areas in
Mexico without the potential for T. cruzi transmission. 相似文献
38.
39.
Lago-Peñas C Casais L Dellal A Rey E Domínguez E 《Journal of strength and conditioning research / National Strength & Conditioning Association》2011,25(12):3358-3367
Lago-Pe?as, C, Casais, L, Dellal, A, Rey, E, and Domínguez, E. Anthropometric and physiological characteristics of young soccer players according to their playing positions: relevance for competition success. J Strength Cond Res 25(12): 3358-3367, 2011-The aim of this study was to establish the anthropometric and physiological profiles of young soccer players according to their playing position and to determine their relevance for competition success. Three hundred and twenty-one young male soccer players participated in the study. Players, age 15.63 (±1.82) years, range 12-19 years, were classified into the following groups: Goalkeepers (n = 35), Central Defenders (n = 53), External Defenders (n = 54), Central Midfielders (n = 61), External Midfielders (n = 46), and Forwards (n = 72). The anthropometric variables of participants (height, weight, body mass index, 6 skinfolds, 4 diameters, and 3 perimeters) were measured. Also, their somatotype and body composition (weights and percentages of fat, bone, and muscle) were calculated. Participants performed the 20-m progressive run test to estimate their relative VO(2)max, a sprint test (30 m flat), and 3 jump tests (squat jump, countermovement jump, and Abalakov test). External Midfielders were the leanest and shortest. In contrast, Central Defenders and Goalkeepers were found to be the tallest and heaviest players. They also had the largest fat skinfolds. In general, the results show that heavier and taller young soccer players performed better in vertical jumps and 30-m sprint, whereas leaner players performed better in the 20-m progressive run test. Players were classified into 2 groups according to the final ranking of their teams at the end of the season. Players from successful teams performed slightly better than players from unsuccessful teams in the physiological test, but these differences were not statistically significant. Moreover, players from successful teams were found to be leaner and more muscular than their unsuccessful counterparts. 相似文献
40.
David F Steinwand M Hust M Bohle K Ross A Dübel S Franco-Lara E 《Biotechnology journal》2011,6(12):1516-1531
Bacillus megaterium was used as an alternative high potential microbial production system for the production of antibody fragment D1.3 scFv. The aim of the study was to follow a holistic optimization approach from medium screening in small scale microtiter platforms, gaining deeper process understanding in the bioreactor scale and implementing advanced process strategies at larger scales (5-100 L). Screening and optimization procedures were supported by statistical design of experiments and a genetic algorithm approach. The process control relied on a soft-sensor for biomass estimation to establish a μ-oscillating time-dependent fed-batch strategy. Several cycles of growth phases and production phases, equal to starving phases, were performed in one production. Flow cytometry was used to monitor and characterize the dynamics of secretion and cell viability. Besides the biosynthesis of the product, secretion was optimized by an appropriate medium design considering different carbon sources, metal ions, (NH(4))(2)SO(4), and inductor concentrations. For bioprocess design, an adapted oscillating fed-batch strategy was conceived and successfully implemented at an industrially relevant scale of 100 L. In comparison to common methods for controlling fed-batch profiles, the developed process delivered increased overall productivities. Thereby measured process parameters such as growth stagnation or productivity fluctuations were directly linked to single cell or population behavior leading to a more detailed process understanding. Above all, the importance of single cell analysis as key scale-free tool to characterize and optimize recombinant protein production is highlighted, since this can be applied to all development stages independently of the cultivation platform. 相似文献