全文获取类型
收费全文 | 114篇 |
免费 | 17篇 |
出版年
2023年 | 2篇 |
2022年 | 2篇 |
2021年 | 3篇 |
2018年 | 3篇 |
2016年 | 1篇 |
2015年 | 8篇 |
2014年 | 4篇 |
2013年 | 6篇 |
2012年 | 9篇 |
2011年 | 5篇 |
2010年 | 2篇 |
2009年 | 4篇 |
2008年 | 4篇 |
2007年 | 6篇 |
2006年 | 5篇 |
2005年 | 4篇 |
2004年 | 1篇 |
2003年 | 3篇 |
2002年 | 5篇 |
2001年 | 4篇 |
2000年 | 1篇 |
1999年 | 1篇 |
1998年 | 2篇 |
1997年 | 1篇 |
1996年 | 5篇 |
1995年 | 3篇 |
1994年 | 1篇 |
1993年 | 1篇 |
1992年 | 1篇 |
1990年 | 1篇 |
1988年 | 1篇 |
1986年 | 3篇 |
1985年 | 1篇 |
1983年 | 3篇 |
1982年 | 4篇 |
1981年 | 1篇 |
1979年 | 1篇 |
1978年 | 2篇 |
1977年 | 4篇 |
1975年 | 4篇 |
1974年 | 1篇 |
1973年 | 1篇 |
1972年 | 1篇 |
1971年 | 1篇 |
1970年 | 1篇 |
1969年 | 1篇 |
1968年 | 2篇 |
1965年 | 1篇 |
排序方式: 共有131条查询结果,搜索用时 31 毫秒
21.
In plants, a large polymorphic family of intracellular NB-LRR receptors lies at the heart of robust resistance to diverse pathogens and mechanisms by which these versatile molecular switches operate in effector-triggered immunity are beginning to emerge. We outline recent advances in our understanding of NB-LRR receptor signaling leading to disease resistance. Themes covered are (i) NB-LRR molecular constraining forces and their intimate relationship with receptor activation in different parts of the cell, (ii) cooperativity between NB-LRR proteins and the formation of higher order NB-LRR signaling complexes, and (iii) the spatial separation of different resistance branches within cells. Finally, we examine evidence for dynamic signaling across cell compartments in coordinating diverse immune outputs. 相似文献
22.
G Sachs J Traugott AP Nesterova G Dell'omo F Kümmeth W Heidrich AL Vyssotski F Bonadonna 《PloS one》2012,7(9):e41449
ALBATROSSES DO SOMETHING THAT NO OTHER BIRDS ARE ABLE TO DO: fly thousands of kilometres at no mechanical cost. This is possible because they use dynamic soaring, a flight mode that enables them to gain the energy required for flying from wind. Until now, the physical mechanisms of the energy gain in terms of the energy transfer from the wind to the bird were mostly unknown. Here we show that the energy gain is achieved by a dynamic flight manoeuvre consisting of a continually repeated up-down curve with optimal adjustment to the wind. We determined the energy obtained from the wind by analysing the measured trajectories of free flying birds using a new GPS-signal tracking method yielding a high precision. Our results reveal an evolutionary adaptation to an extreme environment, and may support recent biologically inspired research on robotic aircraft that might utilize albatrosses' flight technique for engineless propulsion. 相似文献
23.
Wählby C Kamentsky L Liu ZH Riklin-Raviv T Conery AL O'Rourke EJ Sokolnicki KL Visvikis O Ljosa V Irazoqui JE Golland P Ruvkun G Ausubel FM Carpenter AE 《Nature methods》2012,9(7):714-716
We present a toolbox for high-throughput screening of image-based Caenorhabditis elegans phenotypes. The image analysis algorithms measure morphological phenotypes in individual worms and are effective for a variety of assays and imaging systems. This WormToolbox is available through the open-source CellProfiler project and enables objective scoring of whole-worm high-throughput image-based assays of C. elegans for the study of diverse biological pathways that are relevant to human disease. 相似文献
24.
25.
Yiping Fan Guillaume Marcy Eddy S. M. Lee Steve Rozen Citra N. Z. Mattar Simon N. Waddington Eyleen L. K. Goh Mahesh Choolani Jerry K. Y. Chan 《PloS one》2014,9(9)
Neural stem/progenitor cells (NSC) have the potential for treatment of a wide range of neurological diseases such as Parkinson Disease and multiple sclerosis. Currently, NSC have been isolated only from hippocampus and subventricular zone (SVZ) of the adult brain. It is not known whether NSC can be found in all parts of the developing mid-trimester central nervous system (CNS) when the brain undergoes massive transformation and growth. Multipotent NSC from the mid-trimester cerebra, thalamus, SVZ, hippocampus, thalamus, cerebellum, brain stem and spinal cord can be derived and propagated as clonal neurospheres with increasing frequencies with increasing gestations. These NSC can undergo multi-lineage differentiation both in vitro and in vivo, and engraft in a developmental murine model. Regionally-derived NSC are phenotypically distinct, with hippocampal NSC having a significantly higher neurogenic potential (53.6%) over other sources (range of 0%–27.5%, p<0.004). Whole genome expression analysis showed differential gene expression between these regionally-derived NSC, which involved the Notch, epidermal growth factor as well as interleukin pathways. We have shown the presence of phenotypically-distinct regionally-derived NSC from the mid-trimester CNS, which may reflect the ontological differences occurring within the CNS. Aside from informing on the role of such cells during fetal growth, they may be useful for different cellular therapy applications. 相似文献
26.
27.
Melissa Fontes Landell Cheila Denise Ottonelli Stopiglia Raisa G. Billodre Daiane Heidrich Julia Medeiros Sorrentino Marilene H. Vainstein Maria Lúcia Scroferneker Patricia Valente 《Mycopathologia》2011,171(3):203-207
We report a case of a researcher from a laboratory of Mycology in Rio Grande do Sul, Brazil that presented a clinical evidence
of sporotrichosis. The researcher had an accident while manipulating the microculture slides of chromoblastomycosis agents
and presented a clinical evidence of sporotrichosis. As the laboratory has some cultures of Sporothrix schenckii, it was suggested that it might be a laboratory contamination. In order to test this hypothesis, the genotypic characterization
of the samples was performed by means of the random amplified polymorphic DNA (RAPD) analysis method. In addition, we evaluated
the in vitro antifungal activity of four antifungal agents against the isolated fungus. The sample obtained from the researcher
was not genetically similar to any of the samples kept in the laboratory and showed the minimum inhibitory concentrations
of 0.5 μg/mL for itraconazole and ketoconazole, >64 μg/mL for fluconazole and 0.125 μg/mL for terbinafine. It is suggested
that the contamination had an environmental origin. 相似文献
28.
Anika Wranke Benjamin Heidrich Stefanie Ernst Beatriz Calle Serrano Florin Alexandru Caruntu Manuela Gabriela Curescu Kendal Yalcin Selim Gürel Stefan Zeuzem Andreas Erhardt Stefan Lüth George V. Papatheodoridis Birgit Bremer Judith Stift Jan Grabowski Janina Kirschner Kerstin Port Markus Cornberg Christine S. Falk Hans-Peter Dienes Svenja Hardtke Michael P. Manns Cihan Yurdaydin Heiner Wedemeyer HIDIT- Study Group 《PloS one》2014,9(7)
Background
Hepatitis delta frequently leads to liver cirrhosis and hepatic decompensation. As treatment options are limited, there is a need for biomarkers to determine disease activity and to predict the risk of disease progression. We hypothesized that anti-HDV IgM could represent such a marker.Methods
Samples of 120 HDV-infected patients recruited in an international multicenter treatment trial (HIDIT-2) were studied. Anti-HDV IgM testing was performed using ETI-DELTA-IGMK-2-assay (DiaSorin). In addition, fifty cytokines, chemokines and angiogenetic factors were measured using multiplex technology (Bio-Plex System). A second independent cohort of 78 patients was studied for the development of liver-related clinical endpoints (decompensation, HCC, liver transplantation or death; median follow up of 3.0 years, range 0.6–12).Results
Anti-HDV IgM serum levels were negative in 18 (15%), low (OD<0.5) in 76 (63%), and high in 26 (22%) patients of the HIDIT-2 cohort. Anti-HDV IgM were significantly associated with histological inflammatory (p<0.01) and biochemical disease activity (ALT, AST p<0.01). HDV replication was independent from anti-HDV IgM, however, low HBV-DNA levels were observed in groups with higher anti-HDV IgM levels (p<0.01). While high IP-10 (CXCL10) levels were seen in greater groups of anti-HDV IgM levels, various other antiviral cytokines were negatively associated with anti-HDV IgM. Associations between anti-HDV IgM and ALT, AST, HBV-DNA were confirmed in the independent cohort. Clinical endpoints occurred in 26 anti-HDV IgM positive patients (39%) but in only one anti-HDV IgM negative individual (9%; p = 0.05).Conclusions
Serum anti-HDV IgM is a robust, easy-to-apply and relatively cheap marker to determine disease activity in hepatitis delta which has prognostic implications. High anti-HDV IgM levels may indicate an activated interferon system but exhausted antiviral immunity. 相似文献29.
Nicolaas J. R. van Eikema Hommes Dietmar Heidrich Paul von Ragué Schleyer 《Journal of molecular modeling》2000,6(9):563-574
High level ab initio and density functional calculations, extrapolated to QCISD(T)/6-311+G(3df,2p)//MP2/6-31+G**+ZPE, reveal that cyclic ion pairs can form in the hydrogen bonded complexes of haloboric acids BHnX3-n–HX, X=F, Cl, with Lewis bases HX, H2O, CH3OH, and NH3, even in isolation (e.g., in the gas phase). The intrinsic acidities (deprotonation energies) required for protonation of these bases with formation of gas phase ion pairs are calculated to be <295 kcal/mol for water, <301 kcal/mol for methanol, and <306 kcal/mol for ammonia; such values are common for acidic sites in zeolites. All gas phase ion pairs prefer symmetric bidentate or tridentate structures. In the other cases where hydrogen bonded complexes prevail, symmetric ion pair-like transition structures for multiple hydrogen exchange are computed.Supplementary material to this paper is available in electronic form at http://dx.doi.org/10.1007/s0089400060563 相似文献
30.