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91.
Castor bean (Ricinus communis L.) plants were grown for 5–7 weeks in a controlled environment at 350 μl l−1 or 700 μl l−1 CO2. Carbon assimilation, assimilate deposition, dark respiration and assimilate mobilization were measured in leaves 2, 3 and
4 (counted from the base of the plant), and a balance sheet of carbon input and export was elaborated for both CO2 concentrations. Carbon dioxide assimilation was nearly constant over the illumination period, with only a slight depression
occurring at the end of the day in mature source leaves, not in young source leaves. Assimilation was ca. 40% higher at 700 μl l−1 than at 350 μl l−1 CO2. The source leaves increased steadily in weight per unit area during the first 3 weeks, more at 700 μl l−1 than at 350 μl l−1 CO2. On top of an irreversible weight increase, there was a large gain in dry weight during the day, which was reversed during
the night. This reversible weight gain was constant over the life time of the leaf and ca. 80% higher at 700 μl l−1 than at 350 μl l−1. Most of it was due to carbohydrates. The carbon content (as a percentage) was not altered by the CO2 treatment. Respiration was 25% higher in high-CO2 plants when based on leaf area, but the same when based on dry weight. The rate of carbon export via the phloem was the same
during the daytime in plants grown at 350 μl l−1 and 700 μl l−1 CO2. During the night the low-CO2 plants had only 50% of the daytime export rate, in contrast to the high-CO2 plants which maintained the high export rate. It was concluded that the phloem loading system is saturated during the daytime
in both CO2 regimes, whereas during the night the assimilate supply is reduced in plants in the normal CO2 concentration. Two-thirds of the carbon exported from the leaves was permanently incorporated as plant dry matter in the
residual plant parts. This “assimilation efficiency” was the same for both CO2 regimes. It is speculated that under 350 μl l−1 CO2 the growing Ricinus plant operates at sink limitation during the day and at source limitation during the night.
Received: 2 February 1999 / Accepted: 19 April 1999 相似文献
92.
93.
Germination, growth, and physiological responses of hybridizing Carpobrotus from coastal California to soil salinity were studied. Hybrids are presumably the result of hybridization and introgression between the exotic Carpobrotus edulis, a succulent perennial invading coastal habitats, and the native or long-naturalized C. chilensis. Germination responses were investigated at 0, 10, 20, and 50% seawater. Seedling growth and physiology were compared by irrigating seedlings with solutions of the same seawater concentrations and in low and high nutrients. Germination was inhibited in the presence of salt, but recovered after transferring the seeds to fresh water. Seeds exposed to salt had higher final germination rates than control. Growth of Carpobrotus was slightly enhanced by low seawater concentrations but reduced at high salinity at both nutrient regimes. Leaf cell sap osmolarity increased with increasing soil salinity, and taxa did not differ significantly in this physiological adjustment. Leaf carbon isotope ratios (∂13C) ranged from −28 to −22‰ and became less negative at higher salinities, indicating an improved water use efficiency in the seedlings at high salt concentrations. In addition, ∂13C values were generally less negative at high than at low nutrients. Differences among taxa were generally small. The results show that salinity affects both establishment and growth of hybridizing Carpobrotus. The overall weak species differences in salt tolerance indicate that the exotic C. edulis can occupy the same sites as C. chilensis in terms of salinity. The similarity of hybrids in their response to salinity suggests that they may contribute to the invasion by Carpobrotus. 相似文献
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96.
The invasive freshwater snail Tarebia granifera (Lamarck, 1822) was first reported in South Africa in 1999 and it has become widespread across the country, with some evidence to suggest that it reduces benthic macroinvertebrate biodiversity. The current study aimed to identify the primary abiotic drivers behind abundance patterns of T. granifera, by comparing the current abundance of the snail in three different regions, and at three depths, of the highly modified Nseleni River in KwaZulu-Natal, South Africa. Tarebia granifera was well established throughout the Nseleni River system, with an overall preference for shallow waters and seasonal temporal patterns of abundance. Although it is uncertain what the ecological impacts of the snail in this system are, its high abundances suggest that it should be controlled where possible and prevented from invading other systems in the region. 相似文献
97.
Cell-polarity dynamics controls the mechanism of lumen formation in epithelial morphogenesis 总被引:1,自引:0,他引:1
Martín-Belmonte F Yu W Rodríguez-Fraticelli AE Ewald AJ Ewald A Werb Z Alonso MA Mostov K 《Current biology : CB》2008,18(7):507-513
Many organs consist of tubes of epithelial cells enclosing a central lumen. How the space of this lumen is generated is a key question in morphogenesis. Two predominant mechanisms of de novo lumen formation have been observed: hollowing and cavitation. In hollowing, the lumen is formed by exocytosis and membrane separation, whereas, in cavitation, the lumen is generated by apoptosis of cells in the middle of the structure [1, 2]. Using MDCK cells in three-dimensional cultures, we found an inverse correlation between polarization efficiency and apoptosis. When cells were grown in collagen, where cells polarized slowly, apoptosis was needed for lumen formation. However, in the presence of Matrigel, which allowed rapid polarization, lumens formed without apoptosis. If polarization in Matrigel was perturbed by blocking formation of the apical surface by RNAi of Cdc42, lumens formed by apoptosis. In a complementary approach, we plated cells at high density so that aggregates formed with little polarity. These aggregates required apoptosis to form lumens, whereas cells plated at low density formed cysts with rapidly polarizing cells and did not need apoptosis to form lumens. The mechanism of lumen formation in the 3D-MDCK model can shift between hollowing and cavitation, depending on cell polarization. 相似文献
98.
Erwin Märtlbauer Ewald Usleber Richard Dietrich Elisabeth Schneider 《Mycotoxin Research》2009,25(4):175-186
In a long-term study (1990–1997) on ochratoxin A (OTA) in human blood serum, 102 serum samples from 36 persons of the Munich
Institute for Hygiene and Technology of Food of Animal Origin were analysed by enzyme immunoassay (EIA), and by high performance
liquid chromatography (HPLC) for control. Detection limits were at 50 pg/ml (EIA) and 50–70 pg/ml (HPLC), recoveries were
80–120% (EIA) and 30–60% (LC). OTA was detected in 98% (EIA, 368 ± 217 pg/ml) and 93% (HPLC, 271 ± 170 pg/ml) of samples (maximum
1,290 pg/ml). Using published conversion factors for serum/intake estimates (1.34 or 1.97), the mean daily OTA intake of these
36 persons was 493–725 pg/kg bw. Long-term individual mean OTA levels of nine persons ranged from 162 ± 80 pg/ml to 549 ± 172 pg/ml.
Our data were compared with published OTA serum levels (1985–2008) for apparently healthy persons from a total of 30 countries.
On a worldwide basis, the mean of means for OTA in human serum was estimated to be 700 pg/ml, corresponding to a mean daily
OTA intake of 940–1380 pg/kg bw. This level, which was relatively stable over the last decades, is well below published tolerable
daily intake values (14,000–18,000 pg/kg bw). 相似文献
99.
Ritha JA Njau Don de Savigny Lucy Gilson Eleuther Mwageni Franklin W Mosha 《Malaria journal》2009,8(1):1-27
Background
In the past decade there has been increasing visibility of malaria control efforts at the national and international levels. The factors that have enhanced this scenario are the availability of proven interventions such as artemisinin-based combination therapy, the wide scale use of insecticide-treated nets (ITNs) and a renewed emphasis in indoor residual house-spraying. Concurrently, there has been a window of opportunity of financial commitments from organizations such as the Global Fund for HIV/AIDS, Tuberculosis and Malaria (GFATM), the President's Malaria Initiative and the World Bank Booster programme.Methods
The case study uses the health policy analysis framework to analyse the implementation of a public-private partnership approach embarked upon by the government of Tanzania in malaria control – 'The Tanzania National Voucher Scheme'- and in this synthesis, emphasis is on the challenges faced by the scheme during the pre-implementation (2001 – 2004) and implementation phases (2004 – 2005). Qualitative research tools used include: document review, interview with key informants, stakeholder's analysis, force-field analysis, time line of events, policy characteristic analysis and focus group discussions. The study is also complemented by a cross-sectional survey, which was conducted at the Rufiji Health Demographic Surveillance Site, where a cohort of women of child-bearing age were followed up regarding access and use of ITNs.Results
The major challenges observed include: the re-introduction of taxes on mosquito nets and related products, procurement and tendering procedures in the implementation of the GFATM, and organizational arrangements and free delivery of mosquito nets through a Presidential initiative.Conclusion
The lessons gleaned from this synthesis include: (a) the consistency of the stakeholders with a common vision, was an important strength in overcoming obstacles, (b) senior politicians often steered the policy agenda when the policy in question was a 'crisis event', the stakes and the visibility were high, (c) national stakeholders in policy making have an advantage in strengthening alliances with international organizations, where the latter can become extremely influential in solving bottlenecks as the need arises, and (d) conflict can be turned into an opportunity, for example the Presidential initiative has inadvertently provided Tanzania with important lessons in the organization of 'catch-up' campaigns. 相似文献100.
Sandra Skowronek Michael Ewald Maike Isermann Ruben Van De Kerchove Jonathan Lenoir Raf Aerts Jens Warrie Tarek Hattab Olivier Honnay Sebastian Schmidtlein Duccio Rocchini Ben Somers Hannes Feilhauer 《Biological invasions》2017,19(1):239-254
Reliable distribution maps are crucial for the management of invasive plant species. An alternative to traditional field surveys is the use of remote sensing data, which allows coverage of large areas. However, most remote sensing studies on invasive plant species focus on mapping large stands of easily detectable study species. In this study, we used hyperspectral remote sensing data in combination with field data to derive a distribution map of an invasive bryophyte species, Campylopus introflexus, on the island of Sylt in Northern Germany. We collected plant cover data on 57 plots to calibrate the model and presence/absence data of C. introflexus on another 150 plots for independent validation. We simultaneously acquired airborne hyperspectral (APEX) images during summer 2014, providing 285 spectral bands. We used a Maxent modelling approach to map the distribution of C. introflexus. Although C. introflexus is a small and inconspicuous species, we were able to map its distribution with an overall accuracy of 75 %. Reducing the sampling effort from 57 to 7 plots, our models performed fairly well until sampling effort dropped below 12 plots. The model predicts that C. introflexus is present in about one quarter of the pixels in our study area. The highest percentage of C. introflexus is predicted in the dune grassland. Our findings suggest that hyperspectral remote sensing data have the potential to provide reliable information about the degree of bryophyte invasion, and thus provide an alternative to traditional field mapping approaches over large areas. 相似文献