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991.
Cell Division and Deoxyribonucleic Acid Synthesis after a Nutritional Shift-Up of Saccharomyces cerevisiae 总被引:1,自引:1,他引:0
Rates of cell division and deoxyribonucleic acid synthesis after shift-up with grande and mitchondrial deoxyribonucleic-acid-less petite yeasts were studied. The results indicate that simple eukaryotes behave as prokaryotes. 相似文献
992.
Accumulation of 70S Monoribosomes in Escherichia coli After Energy Source Shift-Down 总被引:4,自引:3,他引:1
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When Escherichia coli is shifted from glucose-minimal to succinate-minimal medium, a transient inhibition of protein synthesis and a time-dependent redistribution of ribosomes from polysomes to 70S monosomes occurs. These processes are reversed by a shift-up with glucose. In a lysate made from a mixture of log-phase and down-shifted cells, the 70S monosomes are derived solely from the down-shifted cells and are therefore not produced by polysome breakage during preparation. This conclusion is supported by the absence of nascent proteins from the 70S peak. The monosomes are not dissociated by NaCl or by a crude ribosome dissociation factor, so they behave as "complexed" rather than "free" particles. When down-shifted cells are incubated with rifampin to block ribonucleic acid (RNA) synthesis, the 70S monosomes disappear with a half-life of 15 min. When glucose is also added this half-life decreases to 3 min. The 70S particles are stable in the presence of rifampin when chloramphenicol is added to block protein synthesis. We interpret these data to mean that the existence of the 70S monosomes depends on the continued synthesis of messenger RNA and their conversion to free ribosomes (which dissociate under our conditions) is a result of their participation in protein synthesis. Finally, a significant fraction of the RNA labeled during a brief pulse of (3)H-uracil is found associated with the 70S peak. These results are consistent with the hypothesis that the 70S monosomes are initiation complexes of single ribosomes and messenger RNA, which do not initiate polypeptide synthesis during a shift-down. 相似文献
993.
Control of Stable Ribonucleic Acid Chain Initiation in Escherichia coli During Diauxie Lag 总被引:2,自引:2,他引:0
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Lewis A. Jacobson 《Journal of bacteriology》1972,109(2):678-685
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In mouse parotid membranes forskolin activated adenylate cyclase four-fold; maximal activation of the enzyme occurred with 10 microM forskolin. Activation was not dependent on the guanyl nucleotide GTP nor on the inhibitory guanine nucleotide 5'-0-(2-Thiodiphosphate), GDP beta S. In contrast, stimulation of adenylate cyclase by isoproterenol required GTP and was antagonized by GDP beta S in a dose-dependent manner. These results indicate that the guanyl-binding protein of mouse parotid adenylate cyclase is not a requisite for forskolin activation and lends support for direct interaction of forskolin at the catalytic subunit. 相似文献
1000.
Is Metaphit a phencyclidine antagonist? Studies with ketamine, phencyclidine and N-methylaspartate 总被引:1,自引:0,他引:1
S N Davies J Church J Blake D Lodge R A Lessor K C Rice A E Jacobson 《Life sciences》1986,38(26):2441-2445
The dissociative anaesthetics, phencyclidine and ketamine, block excitation of central neurones by N-methylaspartate. Using the technique of microelectrophoresis on rat spinal neurones in vivo Metaphit, a phencyclidine receptor acylating agent, was tested to see whether it would antagonise this effect of dissociative anaesthetics. The predominant effect of Metaphit was, however, to reduce N-methylaspartate induced excitation. It is concluded that Metaphit has mixed agonist/antagonist effects at the phencyclidine receptor. 相似文献