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11.
N. Muller F. Lapicque E. Drelon P. Gillet C. Monot B. Poletto P. Netter 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》1993,616(2)
Resolution of racemic tiaprofenic acid (TA) has been performed using immobilized human serum albumin as the stationary phase. The eluent was phosphate buffer—acetonitrile—n-octanoic acid (90:10:0.015, v/v). Detection was achieved at 305 nm. The pharmacokinetics of the enantiomers were studied following oral administration into humans and after subcutaneous injection in rats. Plasma concentrations of (+)-TA were much greater than those of (−)-TA. For the rat, the pharmacokinetic parameters between (−)-TA and (+)-TA were all statistically different (p < 0.005). 相似文献
12.
E Drelon J Y Jouzeau P Gillet P Gegout D Chevrier B Terlain P Netter 《Life sciences》1992,51(4):PL19-PL24
The effects of human recombinant interleukin-1 beta (HrIL-1 beta) were investigated in arthritic rats sensitized with type II collagen (CII) and muramyl dipeptide (MDP). When administered subcutaneously (sc) daily during established arthritis, low (0.02 micrograms) and medium (0.2 micrograms) HrIL-1 beta doses exerted paradoxical beneficial properties on paws with moderate and severe inflammation, respectively. In contrast, the highest dose (2 micrograms) had a pejorative effect on developing arthritis. In addition, HrIL-1 beta attenuated paw volume and deterioration of the joints as assessed radiologically. Hence, paw inflammation response to IL-1 exposure depended on the dosage and the severity of previous arthritis prior to the IL-1 challenge. Some of these paradoxical activities may be due to the capacity of IL-1 to induce its own inhibitors or feedback loops thus counterbalancing its phlogistic properties. 相似文献
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Clostridium perfringens epsilon-toxin acts on MDCK cells by forming a large membrane complex. 总被引:2,自引:0,他引:2
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L Petit M Gibert D Gillet C Laurent-Winter P Boquet M R Popoff 《Journal of bacteriology》1997,179(20):6480-6487
Epsilon-toxin is produced by Clostridium perfringens types B and D and is responsible for a rapidly fatal enterotoxemia in animals, which is characterized by edema in several organs due to an increase in blood vessel permeability. The Madin-Darby canine kidney (MDCK) cell line has been found to be susceptible to epsilon-toxin (D. W. Payne, E. D. Williamson, H. Havard, N. Modi, and J. Brown, FEMS Microbiol. Lett. 116:161-168, 1994). Here we present evidence that epsilon-toxin cytotoxic activity is correlated with the formation of a large membrane complex (about 155 kDa) and efflux of intracellular K+ without entry of the toxin into the cytosol. Epsilon-toxin induced swelling, blebbing, and lysis of MDCK cells. Iodolabeled epsilon-toxin bound specifically to MDCK cell membranes at 4 and 37 labeled C and was associated with a large complex (about 155 kDa). The binding of epsilon-toxin to the cell surface was corroborated by immunofluorescence staining. The complex formed at 37 degrees C was more stable than that formed at 4 degrees C, since it was not dissociated by 5% sodium dodecyl sulfate and boiling. 相似文献
15.
Interaction of Gila monster venom with secretin receptors in rat pancreatic membranes 总被引:1,自引:0,他引:1
L. Gillet P. Robberecht M. Waelbroeck J.C. Camus P. De Neef W. König J. Christophe 《Peptides》1984,5(2):407-409
The stimulatory effect of Gila monster venom on adenylate cyclase activity in rat pancreatic membranes was compared to that of porcine secretin and porcine VIP. The maximal effect exerted by the venom was identical to that of VIP but significantly lower than that of secretin. The effect of Gila monster venom could, however, be attributed to its interaction with secretin receptors rather than with VIP receptors, at variance with its previously described action on guinea pig pancreatic acini. Adenylate cyclase activation by both Gila monster venom and secretin in rat pancreatic membranes was, indeed: (1) dose-dependently inhibited by two secretin fragments secretin-(4-27) and secretin-(7-27), and (2) more severely depressed than VIP stimulation, after pretreating pancreatic membranes with dithiothreitol (DTT). 相似文献
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17.
Summary Exchange isotherms of Cu2+
vs Zn2+-ions were performed on the cell wall of a fresh water alga,Nitella flexilis. The relevant thermodynamic parameters are calculated. The cell wall absorbs copper selectively. The selectivity is explained
by a stronger chelation of the cupric ion, due to the Jahn-Teller effect. The wall acts like a two-site model, based on the
nature of the ligands: a first group of aminesites reacts exothermically and a second of hydroxylic-carboxylic sites of lower
affinity, reacts endothermically. 相似文献
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The types of binding of different mono- and divalent ions to sites of the
constitutive pectic acids of the Nitella cell walls
were investigated by performing ion exchanges at different pH. The
experimental results were then analysed in the framework of a model derived
from the polyelectrolyte theory in which the competitive process of
dissociation of the exchange sites and their complexation by counterions
are taken into account. Divalent ions Ca2+ and
Mn2+ interacted specifically with the exchange sites
to give rise to strong thermodynamic association constants. They also
induced conformational transitions of the pectic acids which allowed some
site-specific association with monovalent ions, although the latter, in the
absence of divalent ions, interacted only in a purely electrostatic manner
with the charged sites. The complexation phenomenon of the monovalent ions
also results in a feedback process which enhances or depletes the
site-specific interactions of the divalent counterions. Changes in the
counterion association with the wall exchange sites will take place without
modification in the wall electrostatic field, when divalent ions are
present at the usual pH. These specific interactions are supported by the
values of the residual interaction energy, calculated from the variations
of the apparent pKa of the polygalacturonic acids with
their degree of protonation. 相似文献