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11.
Synthesis and structure-activity relationships of 1,2,4-triazoles as a novel class of potent tubulin polymerization inhibitors 总被引:1,自引:0,他引:1
Ouyang X Chen X Piatnitski EL Kiselyov AS He HY Mao Y Pattaropong V Yu Y Kim KH Kincaid J Smith L Wong WC Lee SP Milligan DL Malikzay A Fleming J Gerlak J Deevi D Doody JF Chiang HH Patel SN Wang Y Rolser RL Kussie P Labelle M Tuma MC 《Bioorganic & medicinal chemistry letters》2005,15(23):5154-5159
A novel triazole-containing chemical series was shown to inhibit tubulin polymerization and cause cell cycle arrest in A431 cancer cells with EC(50) values in the single digit nanomolar range. Binding experiments demonstrated that representative active compounds of this class compete with colchicine for its binding site on tubulin. The syntheses and structure-activity relationship studies for the triazole derivatives are described herein. 相似文献
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13.
Zhongmin Xiang Evgueni E Nesterov Jesse Skoch Tong Lin Bradley T Hyman Timothy M Swager Brian J Bacskai Steven A Reeves 《The journal of histochemistry and cytochemistry》2005,53(12):1511-1516
Current methods for myelin staining in tissue sections include both histological and immunohistochemical techniques. Fluorescence immunohistochemistry, which uses antibodies against myelin components such as myelin basic protein, is often used because of the convenience for multiple labeling. To facilitate studies on myelin, this paper describes a quick and easy method for direct myelin staining in rodent and human tissues using novel near-infrared myelin (NIM) dyes that are comparable to other well-characterized histochemical reagents. The near-infrared fluorescence spectra of these probes allow fluorescent staining of tissue sections in multiple channels using visible light fluorophores commonly used in immunocytochemistry. These dyes have been used successfully to detect normal myelin structure and myelin loss in a mouse model of demyelination disease. 相似文献
14.
Drosophila Bcl-2 proteins participate in stress-induced apoptosis, but are not required for normal development 总被引:1,自引:0,他引:1
Sevrioukov EA Burr J Huang EW Assi HH Monserrate JP Purves DC Wu JN Song EJ Brachmann CB 《Genesis (New York, N.Y. : 2000)》2007,45(4):184-193
Many developing tissues require programmed cell death (PCD) for proper formation. In mice and C. elegans, developmental PCD is regulated by the Bcl-2 family of proteins. Two bcl-2 genes are encoded in the Drosophila genome (debcl/dBorg1/Drob-1/dBok and buffy/dBorg2) and previous RNAi-based studies suggested a requirement for these in embryonic development. However, we report here that, despite the fact that many tissues in fruit flies are shaped by PCD, deletion of the bcl-2 genes does not perturb normal development. We investigated whether the fly bcl-2 genes regulate non-apoptotic processes that require caspases, but found these to be bcl-2 gene-independent. However, irradiation of the mutants demonstrates that DNA damage-induced apoptosis, mediated by Reaper, is blocked by buffy and that debcl is required to inhibit buffy. Our results demonstrate that developmental PCD regulation in the fly does not rely upon the Bcl-2 proteins, but that they provide an added layer of protection in the apoptotic response to stress. 相似文献
15.
JC Poe V Minard-Colin EI Kountikov KM Haas TF Tedder 《Journal of immunology (Baltimore, Md. : 1950)》2012,189(5):2318-2325
Malignant B cells responding to external stimuli are likely to gain a growth advantage in vivo. These cells may therefore maintain surface CD19 expression to amplify transmembrane signals and promote their expansion and survival. To determine whether CD19 expression influences this process, Eμ-Myc transgenic (c-Myc(Tg)) mice that develop aggressive and lethal B cell lymphomas were made CD19 deficient (c-Myc(Tg)CD19(-/-)). Compared with c-Myc(Tg) and c-Myc(Tg)CD19(+/-) littermates, the median life span of c-Myc(Tg)CD19(-/-) mice was prolonged by 81-83% (p < 0.0001). c-Myc(Tg)CD19(-/-) mice also lived 42% longer than c-Myc(Tg) littermates following lymphoma detection (p < 0.01). Tumor cells in c-Myc(Tg) and c-Myc(Tg)CD19(-/-) mice were B lineage derived, had a similar phenotype with a large blastlike appearance, invaded multiple lymphoid tissues, and were lethal when adoptively transferred into normal recipient mice. Importantly, reduced lymphomagenesis in c-Myc(Tg)CD19(-/-) mice was not due to reductions in early B cell numbers prior to disease onset. In mechanistic studies, constitutive c-Myc expression enhanced CD19 expression and phosphorylation on active sites. Reciprocally, CD19 expression in c-Myc(Tg) B cells enhanced c-Myc phosphorylation at regulatory sites, sustained higher c-Myc protein levels, and maintained a balance of cyclin D2 expression over that of cyclin D3. These findings define a new and novel c-Myc:CD19 regulatory loop that positively influences B cell transformation and lymphoma progression. 相似文献
16.
Duncton MA Piatnitski EL Katoch-Rouse R Smith LM Kiselyov AS Milligan DL Balagtas C Wong WC Kawakami J Doody JF 《Bioorganic & medicinal chemistry letters》2006,16(6):1579-1581
A novel class of 1-(isoquinolin-5-yl)-4-arylamino-phthalazines is described as inhibitors of vascular endothelial growth factor receptor II (VEGFR-2). Many compounds display VEGFR-2 inhibitory activity with an IC(50) as low as 0.017 microM in an HTRF enzymatic assay. The compounds also inhibit VEGFR-1, a related tyrosine kinase. 相似文献
17.
Kiselyov AS Semenova M Semenov VV Piatnitski E 《Bioorganic & medicinal chemistry letters》2006,16(6):1726-1730
Novel potent derivatives of (azol-1-yl)methyl-N-arylbenzamides with improved solubility (>3mM) are described as ATP-competitive inhibitors of vascular endothelial growth factor receptor 2 (VEGFR-2). Many compounds display VEGFR-2 inhibitory activity reaching IC(50)<100 nM in the enzymatic assay. The compounds also inhibit the related tyrosine kinase, VEGFR-1, with similar potencies. Several compounds containing bulky lipophilic substituents at the benzamide pharmacophore yielded 10- to 17-fold selectivity for the VEGFR-2 versus VEGFR-1 kinase. 相似文献
18.
Yurgis A. V. Yomantas Irina L. Tokmakova Natalya V. Gorshkova Elena G. Abalakina Svetlana M. Kazakova Evgueni R. Gak Sergey V. Mashko 《Applied and environmental microbiology》2010,76(1):75-83
The isolation of auxotrophic mutants, which is a prerequisite for a substantial genetic analysis and metabolic engineering of obligate methylotrophs, remains a rather complicated task. We describe a novel method of constructing mutants of the bacterium Methylophilus methylotrophus AS1 that are auxotrophic for aromatic amino acids. The procedure begins with the Mu-driven integration of the Escherichia coli gene aroP, which encodes the common aromatic amino acid transporter, into the genome of M. methylotrophus. The resulting recombinant strain, with improved permeability to certain amino acids and their analogues, was used for mutagenesis. Mutagenesis was carried out by recombinant substitution of the target genes in the chromosome by linear DNA using the FLP-excisable marker flanked with cloned homologous arms longer than 1,000 bp. M. methylotrophus AS1 genes trpE, tyrA, pheA, and aroG were cloned in E. coli, sequenced, disrupted in vitro using a Kmr marker, and electroporated into an aroP carrier recipient strain. This approach led to the construction of a set of marker-less M. methylotrophus AS1 mutants auxotrophic for aromatic amino acids. Thus, introduction of foreign amino acid transporter genes appeared promising for the following isolation of desired auxotrophs on the basis of different methylotrophic bacteria.The nonpathogenic Gram-negative bacterium Methylophilus methylotrophus is able to grow efficiently using C1 substrates (methanol, methylamine, or trimethylamine) as the sole source of carbon and energy, and it uses the ribulose monophosphate pathway for fixation of formaldehyde produced by the oxidation of methanol (36). Methanol has received considerable attention by the fermentation industry as an alternative substrate to the more generally used sugars from agricultural crops. It can be synthesized either from petrochemicals or renewable resources, such as biogas (48), and therefore the production of methanol does not compete directly with human food supplies. Methylotrophs can therefore be considered potentially useful strains for industrial biotechnology. M. methylotrophus AS1 is an obligate methylotroph originally isolated from activated sludge, and it has been deposited in the National Collections of Industrial, Marine and Food Bacteria (NCIMB; no. 10515). This organism was extensively studied in the 1970s and has been industrialized on a large scale for the manufacturing of single-cell proteins (SCP) from methanol (56, 63). During that period, a significant amount of research was conducted on the direct production of amino acids by fermentation from methanol (3, 58). Although initially promising, these efforts ultimately proved relatively unsatisfactory and impractical, due primarily to the rather poor set of genetic tools that had been developed for methylotrophs.Over the last 5 years, several genomes of methylotrophs have been sequenced (8, 20, 29, 37, 65, 67), and significant progress in elucidating their metabolism has been achieved (14). The number of tools available for the genetic and metabolic engineering of methylotrophic bacteria has been expanded greatly (1, 15, 21, 43), and strategies to produce fine and bulk chemicals by methylotrophs have been described (5, 42, 57, 61). All of these factors led to renewed interest in the construction of methylotrophic strain producers, and the larger knowledge base has enabled more targeted engineering of these bacteria (55).Although M. methylotrophus AS1 has been extensively studied with regard to the industrial scale production of SCP (56, 63) and the oxidation of methanol at the initial stages of carbon and energy metabolism (13, 28), there has been little metabolic analysis of amino acid biosynthesis in this organism. Moreover, selection of auxotrophic mutants of obligate methylotrophs for broadening convenient genetic tools remains a particularly complicated task (19). Although the isolation of several auxotrophs for M. methylotrophus AS1 has been described (6, 23, 40), their numbers are limited. Development of different methods for the isolation of the mutants did not lead to construction of a collection of auxotrophic mutants that could assist in the investigation of amino acid biosynthetic pathways in M. methylotrophus AS1.As for the l-lysine (Lys) synthesis, systematic research was carried out by specialists at Ajinomoto Co., Inc., Japan, beginning with the investigation of the Lys biosynthetic pathway in M. methylotrophus AS1 (23, 61) and continuing with the construction and improvement of a Lys producer (22, 24, 33, 34). This was followed by optimization of fed-batch fermentation for overproduction of Lys from methanol (35).The aim of our investigation was to generate strains based on M. methylotrophus AS1 with the potential for industrial production of aromatic amino acids (AroAAs). It is known that mutants with relaxed feedback inhibition of key biosynthetic enzymes should be isolated at the initial steps of the construction of the amino acid producers and that the relevant degradation pathways should be blocked due to selection of the corresponding auxotrophic strains (7, 31, 49).In this study, a novel method for the construction of AroAA auxotrophic mutants of M. methylotrophus AS1 is described. This method is based on the introduction of a foreign gene encoding a specific amino acid transporter into the genome of M. methylotrophus AS1. The resulting recombinant methylotrophic strain, which possesses increased permeability to the AroAAs and their analogues, was mutated by recombination-mediated substitution of the target chromosomal genes of aromatic pathways by a flippase recombinase (FLP)-excisable marker from artificial linear DNA. This approach led to the construction of a set of M. methylotrophus AS1 marker-less mutants with destroyed genes of AroAA biosynthesis. Thus, introduction of foreign amino acid transporter genes appeared promising for the following isolation of desired auxotrophs on the basis of different methylotrophic bacteria. 相似文献
19.
Evgueni Doukhanine Christina Gavino Jeffery D. Haines Guillermina Almazan Stéphane Richard 《Molecular biology of the cell》2010,21(17):3029-3040
The quaking viable (qkv) mice represent an animal model of dysmyelination. The absence of expression of the QKI-6 and QKI-7 cytoplasmic isoforms in oligodendrocytes (OLs) during CNS myelination causes the qkv mouse phenotype. The QKI RNA-binding proteins are known to regulate RNA metabolism of cell cycle proteins and myelin components in OLs; however, little is known of their role in reorganizing the cytoskeleton or process outgrowth during OL maturation and differentiation. Here, we identify the actin-interacting protein (AIP)-1 mRNA as a target of QKI-6 by using two-dimensional differential gel electrophoresis. The AIP-1 mRNA contains a consensus QKI response element within its 3′-untranslated region that, when bound by QKI-6, decreases the half-life of the AIP-1 mRNA. Although the expression of QKI-6 is known to increase during OL differentiation and CNS myelination, we show that this increase is paralleled with a corresponding decrease in AIP-1 expression in rat brains. Furthermore, qkv/qkv mice that lack QKI-6 and QKI-7 within its OLs had an increased level of AIP-1 in OLs. Moreover, primary rat OL precursors harboring an AIP-1 small interfering RNA display defects in OL process outgrowth. Our findings suggest that the QKI RNA-binding proteins regulate OL differentiation by modulating the expression of AIP-1. 相似文献
20.
Activation of coagulation factor X (fX) by activated factors IX (fIXa) and VIII (fVIIIa) requires the assembly of the enzyme-cofactor-substrate fIXa-fVIIIa-fX complex on negatively charged phospholipid membranes. Using flow cytometry, we explored formation of the intermediate membrane-bound binary complexes of fIXa, fVIIIa, and fX. Studies of the coordinate binding of coagulation factors to 0.8-microm phospholipid vesicles (25/75 phosphatidylserine/phosphatidylcholine) showed that fVIII (fVIIIa), fIXa, and fX bind to 32 700 +/- 5000 (33 200 +/- 14 100), 20 000 +/- 4500, and 30 500 +/- 1300 binding sites per vesicle with apparent K(d) values of 76 +/- 23 (71 +/- 5), 1510 +/- 430, and 223 +/- 79 nm, respectively. FVIII at 10 nm induced the appearance of additional high-affinity sites for fIXa (1810 +/- 370, 20 +/- 5 nm) and fX (12 630 +/- 690, 14 +/- 4 nm), whereas fX at 100 nm induced high-affinity sites for fIXa (541 +/- 67, 23 +/- 5 nm). The effects of fVIII and fVIIIa on the binding of fIXa or fX were similar. The apparent Michaelis constant of the fX activation by fIXa was a linear function of the fVIIIa concentration with a slope of 1.00 +/- 0.12 and an intrinsic K(m) value of 8.0 +/- 1.5 nm, in agreement with the hypothesis that the reaction rate is limited by the fVIIIa-fX complex formation. In addition, direct correlation was observed between the fX activation rate and formation of the fVIIIa-fX complex. Titration of fX, fVIIIa, phospholipid concentration and phosphatidylserine content suggested that at high fVIIIa concentration the reaction rate is regulated by the concentration of free fX rather than of membrane-bound fX. The obtained results reveal formation of high-affinity fVIIIa-fX complexes on phospholipid membranes and suggest their role in regulating fX activation by anchoring and delivering fX to the enzymatic complex. 相似文献