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761.
Elena Zharkikh Viktor Dremin Evgeny Zherebtsov Andrey Dunaev Igor Meglinski 《Journal of biophotonics》2020,13(10)
The prevalence of diabetes complications is a significant public health problem with a considerable economic cost. Thus, the timely diagnosis of complications and prevention of their development will contribute to increasing the length and quality of patient life, and reducing the economic costs of their treatment. This article aims to review the current state‐of‐the‐art biophotonics technologies used to identify the complications of diabetes mellitus and assess the quality of their treatment. Additionally, these technologies assess the structural and functional properties of biological tissues, and they include capillaroscopy, laser Doppler flowmetry and hyperspectral imaging, laser speckle contrast imaging, diffuse reflectance spectroscopy and imaging, fluorescence spectroscopy and imaging, optical coherence tomography, optoacoustic imaging and confocal microscopy. Recent advances in the field of optical noninvasive diagnosis suggest a wider introduction of biophotonics technologies into clinical practice and, in particular, in diabetes care units. 相似文献
762.
Diversity of Sinorhizobium meliloti from the Central Asian Alfalfa Gene Center 总被引:1,自引:0,他引:1 下载免费PDF全文
Marina L. Roumiantseva Evgeny E. Andronov Larissa A. Sharypova Tatjana Dammann-Kalinowski Mathias Keller J. Peter W. Young Boris V. Simarov 《Applied microbiology》2002,68(9):4694-4697
Sinorhizobium meliloti was isolated from nodules and soil from western Tajikistan, a center of diversity of the host plants (Medicago, Melilotus, and Trigonella species). There was evidence of recombination, but significant disequilibrium, between and within the chromosome and megaplasmids. The most frequent alleles matched those in the published genome sequence. 相似文献
763.
Ivan A. Tarassoff Evgeny V. Kuzmin Igor V. Levchenko Galina N. Zaitseva 《Molecular & general genetics : MGG》1986,205(1):122-126
Summary The occurrence of bacterial promoter-like sequences was shown in the divergent region ofCrithidia oncopelti maxicircular kinetoplast DNA. A promoter-cloning vector based on the neomycin phosphotransferase II (NPT II) gene was used
to localize promoters in three homologous blocks of repeated sequences. The elements found displayed greater promoter strength
inEscherichia coli than the normal promoter of the NPT II gene. Sequences of three promoter-containing inserts from different blocks were determined
and typical prokaryotic promoter sequences were localized. 相似文献
764.
Nataliya R. Rovnyagina Gleb S. Budylin Pavel V. Dyakonov Yuri M. Efremov Marina M. Lipina Yuliya R. Goncharuk Emirkhan E. Murdalov David A. Pogosyan Denis A. Davydov Alexander A. Korneev Natalia B. Serejnikova Karen A. Mikaelyan Stanislav A. Evlashin Vladimir A. Lazarev Alexey V. Lychagin Peter S. Timashev Evgeny A. Shirshin 《Journal of biophotonics》2023,16(3):e202200149
Osteoarthritis (OA) is one of the most common joint diseases worldwide. Unfortunately, clinical methods lack the ability to detect OA in the early stages. Timely detection of the knee joint degradation at the level of tissue changes can prevent its progressive damage. Here, diffuse reflectance spectroscopy (DRS) in the NIR range was used to obtain optical markers of the cartilage damage grades and to assess its mechanical properties. It was observed that the water content obtained by DRS strongly correlates with the cartilage thickness (R = .82) and viscoelastic relaxation time (R = .7). Moreover, the spectral parameters, including water content (OH-band), protein content (CH-band), and scattering parameters allowed for discrimination between the cartilage damage grades (10−4 < P ≤ 10−3). The developed approach may become a valuable addition to arthroscopy, helping to identify lesions at the microscopic level in the early stages of OA and complement the surgical analysis. 相似文献
765.
Mikhail D. Nekrasov Evgeny R. Lukyanenko 《Nucleosides, nucleotides & nucleic acids》2020,39(9):1223-1244
Abstract Herein, we report the synthesis of substituted morpholino nucleoside derivatives starting from ribonucleosides. The present protocol shows high functional group tolerance, uses mild reaction conditions, and gives moderate to good yields. This transformation is based on two sequential pathways: (i) the oxidation of the ribonucleosides to the corresponding dialdehyde using sodium periodate and (ii) the reductive amination of the in situ generated dialdehydes with the hydrochloride salts of various the alkylamines. 相似文献
766.
Gleb S. Budylin Denis A. Davydov Nadezhda V. Zlobina Alexey V. Baev Vyacheslav G. Artyushenko Boris P. Yakimov Evgeny A. Shirshin 《Journal of biophotonics》2022,15(1):e202100268
Quantitative noninvasive assessment of water content in tissues is important for biomedicine. Optical spectroscopy is potentially capable of solving this problem; however, its applicability for clinical diagnostics remains questionable. The presented study compares diffuse reflectance spectroscopy, Raman spectroscopy and multispectral imaging in the characterization of cutaneous edema. The source-detector geometries for each method are selected based on Monte Carlo simulations results to detect the signal from the dermis. Then, the kinetics of the edema development is studied for two models. All methods demonstrate synchronous trends for histamine-induced edema: The water content reaches a maximum of 1 hour after histamine application and then gradually decreases. For the venous occlusion, a 51% increase in water content is observed with Raman spectroscopy. The differences in water content estimation by three methods are explained based on the light propagation model. The obtained results are essential for introducing quantitative optical water measurement technology to the clinics. 相似文献