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91.
Jeanette Ridge Douglas A. Terle Evgenia Dragunsky Inessa Levenbook 《In vitro cellular & developmental biology. Animal》1996,32(4):238-248
Summary Neuroblastomas are neural crest-derived tumors that contain neuronal, melanocyte, and Schwann cell precursors. We examined
the effects of treatment with γ-interferon (γ-IFN) and nerve growth factor (NGF), alone, and in combination, on these progenitor
subpopulations in the human neuroblastoma cell line, SH-SY5Y. Using fluorescence-activated flow cytometry (FACS), changes
in expression of three differentiation-specific or-associated marker proteins, the 200 kD neurofilament protein, the myelin
basic protein, and the S-100 protein, were analyzed. Growth rates and morphological changes associated with each treatment
over the 2-wk incubation period were noted. The greatest effects were observed with combined IFN +NGF treatment. These were
significant increases in expression of all three proteins distinctive morphological signs of differentiation, and extensive
inhibition of proliferation compared to control cultures. Treatment with NGF alone resulted in increased neurofilament protein
expression and in the length and number of neurite extensions, but there was no effect on the growth rate. IFN induced striking
morphological changes, significant inhibition of growth, and changes in protein expression that correlated with neuronal to
non-neuronal subpopulation shifts due to the death of differentiated cells. When treatment was discontinued after 15 d, the
morphological changes induced by NGF were reversed within 2–3 d, while those induced by IFN±NGF were present up to 4 wk post-treatment.
Small, neuroblastic colonies were observed throughout the treatment period and within 4–6 wk after the cessation of treatment
this cell-type fully reconstituted the cultures suggesting the presence of a stem cell. Our results indicate that treatment
with γ-IFN±NGF can regulate growth and induce, either stem cells or progenitor neuronal, Schwann and melanocyte subpopulations
in the SH-SY5Y cell line to irreversibly differentiate. 相似文献
92.
Matveeva EG Gryczynski Z Malicka J Lukomska J Makowiec S Berndt KW Lakowicz JR Gryczynski I 《Analytical biochemistry》2005,344(2):161-167
We present a new approach for performing fluorescence immunoassay in whole blood using fluorescently labeled anti-rabbit immunoglobulin G (IgG) on a silver surface. This approach, which is based on surface plasmon-coupled emission (SPCE), provides increased sensitivity and substantial background reduction due to exclusive selection of the signal from the fluorophores located near a bioaffinity surface. This article describes the effect of an optically dense sample matrix, namely human whole blood and serum, on the intensity of the SPCE. An antigen (rabbit IgG) was adsorbed to a slide covered with a thin silver metal layer, and the SPCE signal from the fluorophore-labeled anti-rabbit antibody, binding to the immobilized antigen, was detected. The effect of the sample matrix (buffer, human serum, or human whole blood) on the end-point immunoassay SPCE signal was studied. It was demonstrated that the kinetics of binding could be monitored directly in whole blood or serum. The results showed that human serum and human whole blood attenuate the SPCE end-point signal and the immunoassay kinetic signal only approximately two- and threefold, respectively, as compared with buffer, resulting in signals that are easily detectable even in whole blood. The high optical absorption of the hemoglobin can be tolerated because only fluorophores within a couple of hundred nanometers from the metallic film contribute to SPCE. Excited fluorophores outside the 200-nm layer do not contribute to SPCE, and their free space emission is not transmitted through the opaque metallic film into the glass substrate. We believe that SPCE has the potential of becoming a powerful approach for performing immunoassays based on surface-bound analytes or antibodies for many biomarkers directly in dense samples such as whole blood with no need for washing steps. 相似文献
93.
Matveeva E Malicka J Gryczynski I Gryczynski Z Lakowicz JR 《Biochemical and biophysical research communications》2004,313(3):721-726
We describe a new method for multi-wavelength immunoassays using surface plasmon-coupled emission (SPCE). This phenomenon is coupling of excited fluorophores with a nearby thin metal film, in our case silver, resulting in strongly directional emission into the underlying glass substrate. The angle at which the radiation propagate through the prism depends on the surface plasmon angle for the relevant wavelength. These angles depend on emission wavelength, allowing measurement of multiple analytes using multiple emission wavelengths. We demonstrated this possibility using antibodies labeled with either Rhodamine Red-X or AlexaFluor 647. These antibodies were directed against an antigen protein bound to the silver surface. The emission from each labeled antibody occurred at a different angle on the glass prism, allowing independent measurement of surface binding of each antibody. This method of SPCE immunoassays can be readily extended to 4 or more wavelengths. 相似文献
94.
Metal-enhanced fluorescence immunoassays using total internal reflection and silver island-coated surfaces 总被引:2,自引:0,他引:2
Matveeva E Gryczynski Z Malicka J Gryczynski I Lakowicz JR 《Analytical biochemistry》2004,334(2):303-311
We present a generic immunoassay platform that uses enhanced total internal reflection fluorescence in the proximity of silver island films (SIFs), a surface coating consisting of metal (silver) particles. This platform is used with a model immunoassay where a protein antigen, rabbit immunoglobulin G, was immobilized on the SIF-coated glass surface. The signal from a fluorescent dye-labeled anti-rabbit antibody binding to the surface antigen was detected; different color dyes have been tested. Close placement of the fluorophore to surface-bound silver nanostructures results in dramatic signal enhancement (up to 40-fold) on the SIFs as compared with the glass slides. Use of the total internal reflection mode of excitation has significant advantages (over classic front-face excitation) for practical assay development. The limited evanescent wave excitation volume makes it possible to minimize the background signal and use the immunoassay with no need for any washing steps. 相似文献
95.
Regulation of telomere length and suppression of genomic instability in human somatic cells by Ku86
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Myung K Ghosh G Fattah FJ Li G Kim H Dutia A Pak E Smith S Hendrickson EA 《Molecular and cellular biology》2004,24(11):5050-5059
Ku86 plays a key role in nonhomologous end joining in organisms as evolutionarily disparate as bacteria and humans. In eukaryotic cells, Ku86 has also been implicated in the regulation of telomere length although the effect of Ku86 mutations varies considerably between species. Indeed, telomeres either shorten significantly, shorten slightly, remain unchanged, or lengthen significantly in budding yeast, fission yeast, chicken cells, or plants, respectively, that are null for Ku86 expression. Thus, it has been unclear which model system is most relevant for humans. We demonstrate here that the functional inactivation of even a single allele of Ku86 in human somatic cells results in profound telomere loss, which is accompanied by an increase in chromosomal fusions, translocations, and genomic instability. Together, these experiments demonstrate that Ku86, separate from its role in nonhomologous end joining, performs the additional function in human somatic cells of suppressing genomic instability through the regulation of telomere length. 相似文献
96.
We established a system for propagation of Sindbis virus (SIN)-based replicons in tissue culture in the form of a tricomponent genome virus. Three RNA fragments containing complementing genetic information required for virus replication are packaged into separate viral particles, and each cell produces at least 1,000 packaged replicons and the number of packaged helpers sufficient to perform the next passage. This system can be used to generate large stocks of packaged replicons. The formation of infectious recombinant SIN virus was not detected in any experiments. These features make multicomponent genome SIN an attractive system for a variety of research and biotechnology applications. 相似文献
97.
Evgenia Gourgari Martin P. Playford Umberto Campia Amit K. Dey Fran Cogen Stephanie Gubb-Weiser Mihriye Mete Sameer Desale Maureen Sampson Allen Taylor Kristina I. Rother Alan T. Remaley Nehal N. Mehta 《Cardiovascular diabetology》2018,17(1):158
Background
Patients with type 1 diabetes (T1DM) have increased mortality from cardiovascular disease (CVD). Risk factors for CVD include an elevation of LDL (LDLp) and small HDL (sHDLp) particles, and a decrease in reverse cholesterol transport i.e. HDL-cholesterol efflux capacity (CEC). Our objective was to compare lipoprotein particles and CEC between T1DM and healthy controls (HC) and to explore the associations between NMR lipid particles and cholesterol efflux.Methods
78 patients with T1DM and 59 HC underwent fasting lipoprotein profile testing by NMR and measurements of CEC by cell-based method. The associations between NMR lipid particles with CEC were analyzed using multivariable linear regression models.Results
Youth with T1DM had higher total LDLp 724 [(563–985) vs 622 (476–794) nmol/L (P?=?0.011)] (Maahs et al. in Circulation 130(17):1532–58, 2014; Shah et al. in Pediatr Diabetes 16(5):367–74, 2015), sHDLp [11.20 (5.7–15.3) vs 7.0 (3.2–13.1) μmol/L (P?=?0.021)], and lower medium HDLp [11.20 (8.5–14.5) vs 12.3 (9–19.4), (P?=?0.049)] and lower CEC (0.98?±?0.11% vs 1.05?±?0.15%, P?=?0.003) compared to HC. Moreover, CEC correlated with sHDLp (β?=???0.28, P?=?0.045) and large HDLp (β?=?0.46, P?<?0.001) independent of age, sex, ethnicity, BMIz, HbA1c, hsCRP and total HDLp in the diabetic cohort.Conclusions
Youth with T1DM demonstrated a more atherogenic profile including higher sHDL and LDLp and lower CEC. Future efforts should focus on considering adding lipoprotein particles and CEC in CVD risk stratification of youth with T1DM.Trial registration Clinical Trials Registration Number NCT0227509198.
Sonja Viljamaa Evgenia Dikareva Jonne Tolonen Jaanika Edesi Kaloian Nickolov Teresa Laitinen Tapio Laakso Risto Korpinen Pekka Saranpää Soile Jokipii-Lukkari Anna Kärkönen Hely Häggman 《Plant Cell, Tissue and Organ Culture》2018,132(2):225-238
Somatic hybridization has been used in potato to overcome the sexual barriers between the cultivated (Solanum tuberosum L.) and wild species. To date hundreds of inter/intra-specific somatic hybrids have been produced via protoplast fusions using 23 Solanum species and characterized for multiple traits such as agronomic, disease/pest resistance, salinity, frost and others. With increasing success in recovery of fusion products, somatic hybrids have been exploited in potato genetics, breeding and genomics studies. Here, we report on progress in somatic hybridization research in potato during the past 40 years. 相似文献
99.
Angeliki Tiptiri-Kourpeti Katerina Spyridopoulou Valentina Santarmaki Georgios Aindelis Evgenia Tompoulidou Eleftheria E. Lamprianidou Georgia Saxami Petros Ypsilantis Evangeli S. Lampri Constantinos Simopoulos Ioannis Kotsianidis Alex Galanis Yiannis Kourkoutas Dimitra Dimitrellou Katerina Chlichlia 《PloS one》2016,11(2)
Probiotic microorganisms such as lactic acid bacteria (LAB) exert a number of strain-specific health-promoting activities attributed to their immunomodulatory, anti-inflammatory and anti-carcinogenic properties. Despite recent attention, our understanding of the biological processes involved in the beneficial effects of LAB strains is still limited. To this end, the present study investigated the growth-inhibitory effects of Lactobacillus casei ATCC 393 against experimental colon cancer. Administration of live Lactobacillus casei (as well as bacterial components thereof) on murine (CT26) and human (HT29) colon carcinoma cell lines raised a significant concentration- and time-dependent anti-proliferative effect, determined by cell viability assays. Specifically, a dramatic decrease in viability of colon cancer cells co-incubated with 109 CFU/mL L. casei for 24 hours was detected (78% for HT29 and 52% for CT26 cells). In addition, live L. casei induced apoptotic cell death in both cell lines as revealed by annexin V and propidium iodide staining. The significance of the in vitro anti-proliferative effects was further confirmed in an experimental tumor model. Oral daily administration of 109 CFU live L. casei for 13 days significantly inhibited in vivo growth of colon carcinoma cells, resulting in approximately 80% reduction in tumor volume of treated mice. Tumor growth inhibition was accompanied by L. casei-driven up-regulation of the TNF-related apoptosis-inducing ligand TRAIL and down-regulation of Survivin. Taken together, these findings provide evidence for beneficial tumor-inhibitory, anti-proliferative and pro-apoptotic effects driven by this probiotic LAB strain. 相似文献
100.
Functional divergence between evolutionary-related LuxG and Fre oxidoreductases of luminous bacteria
Anna A. Deeva Evgenia A. Zykova Elena V. Nemtseva Valentina A. Kratasyuk 《Proteins》2019,87(9):723-729
In luminous bacteria NAD(P)H:flavin-oxidoreductases LuxG and Fre, there are homologous enzymes that could provide a luciferase with reduced flavin. Although Fre functions as a housekeeping enzyme, LuxG appears to be a source of reduced flavin for bioluminescence as it is transcribed together with luciferase. This study is aimed at providing the basic conception of Fre and LuxG evolution and revealing the peculiarities of the active site structure resulted from a functional variation within the oxidoreductase family. A phylogenetic analysis has demonstrated that Fre and LuxG oxidoreductases have evolved separately after the gene duplication event, and consequently, they have acquired changes in the conservation of functionally related sites. Namely, different evolutionary rates have been observed at the site responsible for specificity to flavin substrate (Arg 46). Also, Tyr 72 forming a part of a mobile loop involved in FAD binding has been found to be conserved among Fre in contrast to LuxG oxidoreductases. The conservation of different amino acid types in NAD(P)H binding site has been defined for Fre (arginine) and LuxG (proline) oxidoreductases. 相似文献