Development of a biotechnological procedure for treatment of animal wastes to obtain inexpensive biofertilizer

A biotechnological procedure based on alkaline hydrolysis has been developed for effective processing of various types industrial animal wastes to convert them into a low cost high value biodegradable end product. It was demonstrated in laboratory scale experiments that the bioproduct obtained positively influenced microbial soil populations and ryegrass growth in both soils used, park soil and mine spoils. As the remaining partially degraded waste residue is highly dispersed it should act as a slow release fertilizer, thus feeding plants additionally. Therefore, the protein hydrolysate obtained is suitable for application as a harmless and valuable biofertilizer. The proposed method for solubilization of proteinaceous wastes is simple and economically viable making its implementation easier on an industrial scale. Therefore, the method could be of great help to the enterprises of the meat industry, avoiding using landfills and incineration.     

Ontogenetic variation in occlusal shape of evergrowing molars in voles: An intravital study in Microtus gregalis (Arvicolinae,Rodentia)

In order to reveal patterns of ontogenetic change in occlusal shape of evergrowing molars in arvicolines, an intravital tooth printing method was applied to 20 individuals of Microtus gregalis born in captivity. Complexity patterns of the first lower molar were assessed by morphotype analysis of the anteroconid complex. Morphotype dental patterns were monitored using tooth prints at 0.5, 1, 2, 3 months of age, and postmortem. Ontogenetic changes in molar complexity and bilateral symmetry among right and left molars of the same individual during the process of tooth wear were assessed. Our results suggested that morphotype dental patterns could not be clearly established in half-month old animals due to presence of juvenile characters. For animals of 1 month and older, age differences in morphotype dental patterns were non-significant and negligible compared to among-individual variation. Within-individual differences among right and left molars, when present, were not related to age of an animal suggesting that bilateral asymmetry of morphotype dental pattern could be regarded as inherent characteristic of an individual persisting during post-juvenile tooth wear.     

What to expect when you are consolidating: effective prediction models of application performance on multicores

Consolidation of multiple applications with diverse and changing resource requirements is common in multicore systems as hardware resources are abundant. As opportunities for better system usage become ample, so are opportunities to degrade individual application performances due to unregulated performance interference between applications and system resources. Can we predict a performance region within which application performance is expected to lie under different consolidations? Alternatively, can we maximize resource utilization while maintaining individual application performance targets? In this work we provide a methodology that offers answers to the above difficult questions by constructing a queueing-theory based tool that can be used to accurately predict application scalability on multicores. The tool can also provide the optimal consolidation suggestions to maximize system resource utilization while meeting application performance targets. The proposed methodology is based on asymptotic analysis that can quickly provide a range of performance values that the user should expect under various consolidation scenarios. In addition, when more accurate performance forecasting is needed, the methodology can provide more accurate predictions using approximate mean value analysis. The methodology is light-weight as it relies on capturing application resource demands using standard system monitoring, via non-intrusive low-level measurements. We evaluate our approach on an IBM Power7 system using the DaCapo and SPECjvm2008 benchmark suites. From 900 different consolidations of application instances, our tool accurately predicts the average iteration time of collocated applications with an average error below 9 per cent. Experimental and analytical results are in excellent agreement, confirming the robustness of the proposed methodology in suggesting the best consolidations that meet given performance objectives of individual applications while maximizing system resource utilization.     

Leukemia incidence among people exposed to chronic radiation from the contaminated Techa River, 1953–2005

Beginning in 1950, people living on the banks of the Techa River received chronic low-dose-rate internal and external radiation exposures as a result of releases from the Mayak nuclear weapons plutonium production facility in the Southern Urals region of the Russian Federation. The Techa River cohort includes about 30,000 people who resided in riverside villages sometime between 1950 and 1960. Cumulative red bone marrow doses range up to 2 Gy with a mean of 0.3 Gy and a median of 0.2 Gy. Between 1953 and 2005, 93 first primary cases of leukemia, including 23 cases of chronic lymphatic leukemia (CLL), were ascertained among the cohort members. A significant linear dose–response relationship was seen for leukemias other than CLL (P < 0.001), but not for CLL. The estimated excess relative risk per Gy is 4.9 (95% confidence interval (CI): 1.6; 14.3) for leukemias other than CLL and less than 0 (95% upper bound 1.4) for CLL.     

Does Intrinsic Disorder in Proteins Favor Their Interaction with Lipids?

Intrinsically disordered proteins (IDPs) are implicated in a range of human diseases, some of which are associated with the ability to bind to lipids. Although the presence of solvent‐exposed hydrophobic regions in IDPs should favor their interactions with low‐molecular‐weight hydrophobic/amphiphilic compounds, this hypothesis has not been systematically explored as of yet. In this study, the analysis of the DisProt database with regard to the presence of lipid‐binding IDPs (LBIDPs) reveals that they comprise, at least, 15% of DisProt entries. LBIDPs are classified into four groups by ligand type, functional categories, domain structure, and conformational state. 57% of LBIDPs are classified as ordered according to the CH‐CDF analysis, and 70% of LBIDPs possess lengths of disordered regions below 50%. To investigate the lipid‐binding properties of IDPs for which lipid binding is not reported, three proteins from different conformational groups are rationally selected. They all are shown to bind linoleic (LA) and oleic (OA) acids with capacities ranging from 9 to 34 LA/OA molecules per protein molecule. The association with LA/OA causes the formation of high‐molecular‐weight lipid–protein complexes. These findings suggest that lipid binding is common among IDPs, which can favor their involvement in lipid metabolism.     

Thermodynamics of RNA melting,one base pair at a time

The melting of base pairs is a ubiquitous feature of RNA structural transitions, which are widely used to sense and respond to cellular stimuli. A recent study employing solution nuclear magnetic resonance (NMR) imino proton exchange spectroscopy provides a rare base-pair-specific view of duplex melting in the Salmonella FourU RNA thermosensor, which regulates gene expression in response to changes in temperature at the translational level by undergoing a melting transition. The authors observe “microscopic” enthalpy–entropy compensation—often seen “macroscopically” across a series of related molecular species—across base pairs within the same RNA. This yields variations in base-pair stabilities that are an order of magnitude smaller than corresponding variations in enthalpy and entropy. A surprising yet convincing link is established between the slopes of enthalpy–entropy correlations and RNA melting points determined by circular dichroism (CD), which argues that unfolding occurs when base-pair stabilities are equalized. A single AG-to-CG mutation, which enhances the macroscopic hairpin thermostability and folding cooperativity and renders the RNA thermometer inactive in vivo, spreads its effect microscopically throughout all base pairs in the RNA, including ones far removed from the site of mutation. The authors suggest that an extended network of hydration underlies this long-range communication. This study suggests that the deconstruction of macroscopic RNA unfolding in terms of microscopic unfolding events will require careful consideration of water interactions.