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141.
Walter Cañon-Mancisidor Evgenia Spodine Veronica Paredes-Garcia Diego Venegas-Yazigi 《Journal of molecular modeling》2013,19(7):2835-2844
A theoretical study of the magnetic properties, using density functional theory, of a family of trinuclear μ3-OH copper(II) complexes reported in the literature is presented. The reported X-ray crystal structures of [Cu3(μ3-OH)(aat)3(H2O)3](NO3)2·H2O (HUKDUM), where aat: 3-acetylamine-1,2,4-triazole; [Cu3(μ3-OH)(aaat)3(H2SO4)(HSO4)(H2O)] (HUKDOG), where aaat: 3-acetylamine-5-amine-1,2,4-triazole; [Cu3(μ3-OH)(PhPyCNO)3(tchlphac)2] (HOHQUR), where PhPyCNO: phenyl 2-pyridyl-ketoxime and tchlphac: acid 2,4,5-trichlorophenoxyacetic; [Cu3(μ3-OH)(PhPyCNO)3(NO3)2(CH3OH)] (ILEGEM); [Cu3(μ3-OH)(pz)3(Hpz)3(ClO4)2] (QOPJIP), where Hpz?=?pyrazole; [Cu3(μ3-OH)(pz)3(Hpz)(Me3CCOO)2]?2Me3CCOOH (DEFSEN) and [Cu3(μ3-OH)(8-amino-4-methyl-5-azaoct-3-en-2-one)3][CuI3] (RITXUO), were used in the calculations. The magnetic exchange constants were calculated using the broken-symmetry approach. The calculated J values are for HUKDUM J1?=??68.6 cm?1, J2?=??69.9 cm?1, J3?=??70.4 cm?1; for HUKDOG, J1?=??73.5 cm?1, J2?=??58.9 cm?1, J3?=??62.1 cm?1; for HOHQUR J1?=??128.3 cm?1, J2?=??134.1 cm?1, J3?=??120.4 cm?1; for ILEGEM J1?=??151.6 cm?1, J2?=??173.9 cm?1, J3?=??186.9 cm?1; for QOPJIP J1?=??118.3 cm?1, J2?=??106.0 cm?1, J3?=??120.6 cm?1; for DEFSEN J1?=??74.9 cm?1, J2?=??64.0 cm?1, J3?=??57.7 cm?1 and for RITXUO J1?=??10.9 cm?1, J2?=?+14.3 cm?1, J3?=??35.4 cm?1. The Kahn-Briat model was used to correlate the calculated magnetic properties with the overlap of the magnetic orbitals. Spin density surfaces show that the delocalization mechanism is predominant in all the studied compounds. Figure
The Kahn-briat model was used to correlate the calculated magnetic properties with the overlap of the magnetic orbitals. 相似文献
142.
Davide Vigetti Manuela Rizzi Paola Moretto Sara Deleonibus Jonathan M. Dreyfuss Evgenia Karousou Manuela Viola Moira Clerici Vincent C. Hascall Marco F. Ramoni Giancarlo De Luca Alberto Passi 《The Journal of biological chemistry》2011,286(40):34497-34503
Smooth muscle cells (SMCs) have a pivotal role in cardiovascular diseases and are responsible for hyaluronan (HA) deposition in thickening vessel walls. HA regulates SMC proliferation, migration, and inflammation, which accelerates neointima formation. We used the HA synthesis inhibitor 4-methylumbelliferone (4-MU) to reduce HA production in human aortic SMCs and found a significant increase of apoptotic cells. Interestingly, the exogenous addition of HA together with 4-MU reduced apoptosis. A similar anti-apoptotic effect was observed also by adding other glycosaminoglycans and glucose to 4-MU-treated cells. Furthermore, the anti-apoptotic effect of HA was mediated by Toll-like receptor 4, CD44, and PI3K but not by ERK1/2. 相似文献
143.
Polyoxometalate cluster [V12B18O60H6] functionalized with the copper(II) bis-ethylenediamine complex
Kareen Brown Pierre Emmanuel Car Andrés Vega Diego Venegas-Yazigi Verónica Paredes-García María G.F. Vaz Jean-Yves Pivan Eric Le Fur Evgenia Spodine 《Inorganica chimica acta》2011,367(1):21-28
Three compounds based on the polyoxometalate building block [V12B18O60H6], (Na)10[(H2O)V12B18O60H6]·18H2O (1), Na8[Cu(en)2]2[V12B18O60H6](NO3)2·14.7H2O (2), Na7[Cu(en)2]2[V12B18O60H6](NO3)·15.5H2O (3), (en = ethylenediamine), have been hydrothermally synthesized and characterized by single-crystal X-ray diffraction analysis and TGA. Compound 1 consists of polyoxovanadium borate [V12B18O60H6] clusters which are surrounded by sodium countercations in octahedral sites, stabilized by electrostatic interactions with the oxygen atoms of both vanadium and boron centres. However, compounds 2 and 3 correspond to more complicated structures, constructed from the same polyoxometalate clusters, which are interconnected by [Cu(en)2]2+ moieties via the terminal oxygen atoms of the polyoxoanions, generating one-dimensional structures. The functionalization of this polyoxovanadium borate cluster has been obtained by the use of [Cu(en)2]2+ complex ions, thus demonstrating the capacity of the terminal oxygen atoms of the cluster to bind transition metal centres. The structural stability of the [V12B18O60H6] cluster permits the formation of functionalized polyoxometalate clusters, generating various crystalline lattices. 相似文献
144.
145.
Thilo Rühle Jafar Angouri Razeghi Evgenia Vamvaka Stefania Viola Chiara Gandini Tatjana Kleine Danja Schünemann Roberto Barbato Peter Jahns Dario Leister 《Plant physiology》2014,165(1):207-226
The chloroplast F1Fo-ATP synthase/ATPase (cpATPase) couples ATP synthesis to the light-driven electrochemical proton gradient. The cpATPase is a multiprotein complex and consists of a membrane-spanning protein channel (comprising subunit types a, b, b′, and c) and a peripheral domain (subunits α, β, γ, δ, and ε). We report the characterization of the Arabidopsis (Arabidopsis thaliana) CONSERVED ONLY IN THE GREEN LINEAGE160 (AtCGL160) protein (AtCGL160), conserved in green algae and plants. AtCGL160 is an integral thylakoid protein, and its carboxyl-terminal portion is distantly related to prokaryotic ATP SYNTHASE PROTEIN1 (Atp1/UncI) proteins that are thought to function in ATP synthase assembly. Plants without AtCGL160 display an increase in xanthophyll cycle activity and energy-dependent nonphotochemical quenching. These photosynthetic perturbations can be attributed to a severe reduction in cpATPase levels that result in increased acidification of the thylakoid lumen. AtCGL160 is not an integral cpATPase component but is specifically required for the efficient incorporation of the c-subunit into the cpATPase. AtCGL160, as well as a chimeric protein containing the amino-terminal part of AtCGL160 and Synechocystis sp. PCC6803 Atp1, physically interact with the c-subunit. We conclude that AtCGL160 and Atp1 facilitate the assembly of the membranous part of the cpATPase in their hosts, but loss of their functions provokes a unique compensatory response in each organism.The majority of cellular energy is stored in the form of ATP synthesized by the ubiquitous F1Fo-ATP synthase (F1 stands for coupling factor 1, Fo for coupling factor o), which is found in the energy-transducing membranes of bacteria, mitochondria, and chloroplasts. The chloroplast F1Fo-ATP synthase/ATPase (cpATPase) is a rotary motor that is responsible for coupling ATP synthesis (and hydrolysis) to the light-driven electrochemical proton gradient. The cpATPase comprises two physically separable parts, chloroplast coupling factor o (CFo), which is an integral membrane-spanning proton channel, and chloroplast coupling factor 1 (CF1), which is located peripheral to the membrane and contains the catalytic site(s) for reversible ATP synthesis (for review, see von Ballmoos et al., 2009). CFo comprises four different subunit types, designated b (synonymously, I or AtpF), b′ (II or AtpG), c (III or AtpH), and a (IV or AtpI), and contains one each of subunits a, b, and b′ and a ring made up of 14 copies of subunit c. CF1 comprises five different subunits, α (AtpA), β (AtpB), γ (AtpC), δ (AtpD), and ε (AtpE), and its subunit composition is α3β3γδε (for review, see von Ballmoos et al., 2009).The passage of protons through the CFo motor drives rotation of the ring of c-subunits, which together form a rotor. The c-ring is connected to subunit γ, and rotation of γ causes conformational changes in the catalytic nucleotide-binding sites of the CF1 motor, resulting in the synthesis and release of ATP (for review, see Okuno et al., 2011). This process is made possible by the fact that CF1 and CFo are physically connected by two stalks, a central one containing the ε- and γ-subunits and a peripheral one made up of δ, b, and b′ (for review, see Böttcher and Gräber, 2000; Weber, 2007). There are six nucleotide-binding sites in CF1, one at each of the αβ-subunit interfaces about halfway along the vertical axis of the hexamer. Three of the sites are located primarily on the β-subunits and are catalytic; the other three are noncatalytic and probably regulatory. While the three-dimensional structure of the α3β3 hexamer in chloroplasts has been solved to a resolution of 3.2 Å (Groth and Pohl, 2001), the structure of the entire CFo has not yet been determined. However, the conformation of the ring-forming part of CFo from spinach (Spinacia oleracea) chloroplasts has been defined and found to consist of 14 c-units (Vollmar et al., 2009), whereas the c-ring of the ATP synthase from the cyanobacterium Spirulina platensis contains 15 units (Pogoryelov et al., 2009).Similar to other thylakoid multiprotein complexes like PSII and PSI as well as the cytochrome b6f complex (Cyt b6f), the assembly of the ATP synthase must be tightly regulated. Moreover, the variable stoichiometry of the constituents of F1 (three α/β-subunits versus one each of γ, δ, and ε) and Fo (10–15 c-subunits versus one each of a, b, and b′) requires coordination of the expression of the corresponding genes. This is particularly important in eukaryotes, where the genes are located in different compartments, for instance, in the case of the cpATPase, in the plastid (for α, β, ε, a, b, and c) and the nucleus (for b′, γ, and δ).The assembly of ATP synthase has been most extensively studied in Saccharomyces cerevisiae mitochondria, leading to the identification of several factors involved in this process (for review, see Rak et al., 2009). Thus, three proteins in yeast are known to be involved in the assembly of the α3β3 hexamer of F1. Atp11p (Ackerman and Tzagoloff, 1990a; Wang and Ackerman, 1996) and Atp12p (Ackerman and Tzagoloff, 1990a; Wang and Ackerman, 1998) code for mitochondrial proteins that interact with the β- and α-subunits, respectively, to promote their assembly into the oligomeric F1-ATPase, and the absence of either protein causes the α- and β-subunits to aggregate into insoluble inclusion bodies in the mitochondrial matrix. Lack of the third protein, FORMATION OF MITOCHONDRIAL COMPLEXES1 (Fmc1p), is associated with aggregation of the α- and β-subunits under heat stress, suggesting that Fmc1p is required for correct folding of Atp12p at elevated temperatures (Lefebvre-Legendre et al., 2001). Originally, the c-ring was assumed to form spontaneously (Arechaga et al., 2002), but subsequent studies have indicated that the assembly of this structural component is also a protein-assisted process. Thus, Atp25p is required for both the synthesis of the c-subunit and its oligomerization into a ring structure of the proper size (Zeng et al., 2008). Moreover, Atp10p (Ackerman and Tzagoloff, 1990b), Atp23p (Osman et al., 2007), and OXIDASE ASSEMBLY1 (Oxa1p) (Jia et al., 2007) are involved in Fo assembly in yeast mitochondria.In prokaryotes, two ATP synthase assembly factors have been described in detail. The membrane protein insertase YidC belongs to the Oxa1 family, is required in vitro for the membrane insertion of subunit c, and assists in the formation of the c-ring from monomers (van der Laan et al., 2004; Kol et al., 2008). In bacterial genomes, the atp1/uncI genes typically precede the genes encoding the structural subunits of the F1Fo-ATP synthase (for review, see Kol et al., 2008). Moreover, in Synechocystis sp. PCC6803, sll1321/atp1 is coordinately expressed with the seven other genes in the ATP synthase operon (Grossman et al., 2010), implying that Sll1321/Atp1 might have a function associated with the ATP synthase. The genes atp1 and uncI code for small proteins; for instance, Synechocystis sp. PCC6803 Sll1321 has 117 amino acids, and Escherichia coli UncI has 130 amino acids. The function of Atp1/UncI has long remained elusive because deletion of uncI in E. coli results merely in a slightly reduced growth yield (Gay, 1984), indicating that the protein is not essential for the formation of the F1Fo-ATP synthase complex. Similarly, in the alkaliphilic Bacillus pseudofirmus OF4, Atp1/UncI is not absolutely required for ATP synthase function, and a B. pseudofirmus strain deleted for the atp1 gene could still grow nonfermentatively and its purified ATP synthase had a c-ring of normal size (Liu et al., 2013). Recently, a hybrid F1Fo (F1 from Bacillus PS3 and Fo from Propionigenium modestum) was expressed in E. coli. In this system, P. modestum Atp1/UncI was found to be indispensable for c-ring formation and coupled ATPase activity (Suzuki et al., 2007). Similarly, functional production of the Na+ F1Fo-ATP synthase from Acetobacterium woodii in E. coli required the A. woodii atp1/uncI gene for proper assembly (Brandt et al., 2013). Moreover, because subunit c monomers, as well as assembled c-rings, can be copurified together with P. modestum UncI/Atp1 (Suzuki et al., 2007) and the oligomerization of P. modestum c-subunits into c11-rings is mediated by Atp1/UncI in vitro (Ozaki et al., 2008), Atp1/UncI seems to play a role in c-ring assembly for some bacterial ATP synthases.In plants and green algae, regulation of the biogenesis of the cpATPase is well understood at the level of translation of CF1 subunits (Drapier et al., 2007). Thus, synthesis of the nucleus-encoded subunit γ is required for sustained translation of the chloroplast-encoded subunit β, which in turn transactivates the translation of chloroplast-encoded subunit α. Translational down-regulation of subunit β or α, when not assembled, involves the 5′ untranslated regions (UTRs) of their own mRNAs, pointing to control at the level of translation initiation. In addition, a negative feedback exerted by α/β assembly intermediates on the translation of subunit β can be released when subunit γ assembles with α3β3 hexamers.Our knowledge of the nature of true assembly factors for the cpATPase is scarce. So far, only the ALBINO3 homolog Alb4 protein, which can functionally substitute for YidC in E. coli, has been shown to play a role in the biogenesis of the cpATPase, possibly by stabilizing or promoting the assembly of CF1 during its attachment to the CFo portion (Benz et al., 2009). Thus, Alb4-Oxa1p-YidC represents an ATP synthase assembly factor family that is conserved between prokaryotes, yeast, and plants. For the bacterial Atp1/UncI protein, one homolog exists in yeast, Vma21p, which is an integral membrane protein localized to the endoplasmic reticulum and is required for vacuolar H+-ATPase biogenesis (Graham et al., 1998).In this study, we have identified and characterized a knockout mutant for Arabidopsis (Arabidopsis thaliana) CGL160, a protein that displays moderate similarity to prokaryotic Atp1/UncI proteins in its C-terminal domain. AtCGL160 is required for the efficient assembly of the cpATPase, but lack of AtCGL160 in Arabidopsis has more severe effects on cpATPase assembly than those reported in the literature for inactivation of its prokaryotic relatives and can be located to the assembly of c-subunits into the membranous subcomplex. AtCGL160 physically interacts with the c-subunit of CFo, and, interestingly, Atp1 can replace the C-terminal part of AtCGL160 in such interactions, indicating that the function of Atp1 and CGL160 proteins is conserved. 相似文献
146.
Evgenia Blagodatskaya Xiaoming Zou Xingliang Xu Yakov Kuzyakov 《Global Change Biology》2014,20(6):1943-1954
Increase of belowground C allocation by plants under global warming or elevated CO2 may promote decomposition of soil organic carbon (SOC) by priming and strongly affects SOC dynamics. The specific effects by priming of SOC depend on the amount and frequency of C inputs. Most previous priming studies have investigated single C additions, but they are not very representative for litterfall and root exudation in many terrestrial ecosystems. We evaluated effects of 13C‐labeled glucose added to soil in three temporal patterns: single, repeated, and continuous on dynamics of CO2 and priming of SOC decomposition over 6 months. Total and 13C labeled CO2 were monitored to analyze priming dynamics and net C balance between SOC loss caused by priming and the retention of added glucose‐C. Cumulative priming ranged from 1.3 to 5.5 mg C g?1 SOC in the subtropical, and from ?0.6 to 5.5 mg C g?1 SOC in the tropical soils. Single addition induced more priming than repeated and continuous inputs. Therefore, single additions of high substrate amounts may overestimate priming effects over the short term. The amount of added glucose C remaining in soil after 6 months (subtropical: 8.1–11.2 mg C g?1 SOC or 41‐56% of added glucose; tropical: 8.7–15.0 mg C g?1 SOC or 43–75% of glucose) was substantially higher than the net C loss due to SOC decomposition including priming effect. This overcompensation of C losses was highest with continuous inputs and lowest with single inputs. Therefore, raised labile organic C input to soils by higher plant productivity will increase SOC content even though priming accelerates decomposition of native SOC. Consequently, higher continuous input of C belowground by plants under warming or elevated CO2 can increase C stocks in soil despite accelerated C cycling by priming in soils. 相似文献
147.
Soil C and N availability determine the priming effect: microbial N mining and stoichiometric decomposition theories 总被引:15,自引:0,他引:15
Ruirui Chen Mehmet Senbayram Sergey Blagodatsky Olga Myachina Klaus Dittert Xiangui Lin Evgenia Blagodatskaya Yakov Kuzyakov 《Global Change Biology》2014,20(7):2356-2367
The increasing input of anthropogenically derived nitrogen (N) to ecosystems raises a crucial question: how does available N modify the decomposer community and thus affects the mineralization of soil organic matter (SOM). Moreover, N input modifies the priming effect (PE), that is, the effect of fresh organics on the microbial decomposition of SOM. We studied the interactive effects of C and N on SOM mineralization (by natural 13C labelling adding C4‐sucrose or C4‐maize straw to C3‐soil) in relation to microbial growth kinetics and to the activities of five hydrolytic enzymes. This encompasses the groups of parameters governing two mechanisms of priming effects – microbial N mining and stoichiometric decomposition theories. In sole C treatments, positive PE was accompanied by a decrease in specific microbial growth rates, confirming a greater contribution of K‐strategists to the decomposition of native SOM. Sucrose addition with N significantly accelerated mineralization of native SOM, whereas mineral N added with plant residues accelerated decomposition of plant residues. This supports the microbial mining theory in terms of N limitation. Sucrose addition with N was accompanied by accelerated microbial growth, increased activities of β‐glucosidase and cellobiohydrolase, and decreased activities of xylanase and leucine amino peptidase. This indicated an increased contribution of r‐strategists to the PE and to decomposition of cellulose but the decreased hemicellulolytic and proteolytic activities. Thus, the acceleration of the C cycle was primed by exogenous organic C and was controlled by N. This confirms the stoichiometric decomposition theory. Both K‐ and r‐strategists were beneficial for priming effects, with an increasing contribution of K‐selected species under N limitation. Thus, the priming phenomenon described in ‘microbial N mining’ theory can be ascribed to K‐strategists. In contrast, ‘stoichiometric decomposition’ theory, that is, accelerated OM mineralization due to balanced microbial growth, is explained by domination of r‐strategists. 相似文献
148.
Nathan Lawlor Eladio J. Márquez Peter Orchard Narisu Narisu Muhammad Saad Shamim Asa Thibodeau Arushi Varshney Romy Kursawe Michael R. Erdos Matt Kanke Huiya Gu Evgenia Pak Amalia Dutra Sheikh Russell Xingwang Li Emaly Piecuch Oscar Luo Peter S. Chines Michael L. Stitzel 《Cell reports》2019,26(3):788-801.e6
149.
Marina Yu. Skorkina Evgenia A. Sladkova Elena A. Shamray Olga V. Cherkashina Maxim P. Evstigneev Anatoly S. Buchelnikov Yuriy I. Prylutskyy Uwe Ritter 《European biophysics journal : EBJ》2015,44(6):493-498
The influence of aqueous solution of pristine C60 fullerene (C60FAS) on functional activity of lymphocytes from a healthy person was studied for the first time. By means of atomic force microscopy, it was found that C60FAS in a concentration of 0.1 mg/ml increases the stiffness of the lymphocyte membrane by 41 % (p < 0.05) and lowers the functional activity of the plasmalemma surface, thereby constraining the use of its membrane material in physiological reactions using a hypotonic model in vitro. However, a cell retains the ability to regulate its volume and demonstrates relative resistance to hypo-osmotic stress. The resistance of lymphocytes in hypo-osmotic medium is facilitated by activation of the nucleus by C60 fullerene particles, which regulates the implementation of two consistent phases of an increase and decrease of cell volume, thereby retaining cell viability. All these indicate the impact of C60 fullerene on the cellular nucleus. 相似文献
150.
Vladimir Isachenko Plamen Todorov Evgenia Isachenko Gohar Rahimi Andrey Tchorbanov Nikolina Mihaylova Iliyan Manoylov Peter Mallmann Markus Merzenich 《PloS one》2015,10(6)