全文获取类型
收费全文 | 633篇 |
免费 | 117篇 |
出版年
2021年 | 10篇 |
2017年 | 9篇 |
2016年 | 9篇 |
2015年 | 13篇 |
2014年 | 23篇 |
2013年 | 32篇 |
2012年 | 35篇 |
2011年 | 37篇 |
2010年 | 29篇 |
2009年 | 13篇 |
2008年 | 22篇 |
2007年 | 21篇 |
2006年 | 21篇 |
2005年 | 22篇 |
2004年 | 28篇 |
2003年 | 15篇 |
2002年 | 23篇 |
2001年 | 9篇 |
2000年 | 13篇 |
1999年 | 16篇 |
1998年 | 6篇 |
1997年 | 7篇 |
1996年 | 8篇 |
1994年 | 7篇 |
1993年 | 9篇 |
1992年 | 12篇 |
1991年 | 10篇 |
1990年 | 12篇 |
1989年 | 11篇 |
1988年 | 6篇 |
1987年 | 10篇 |
1986年 | 10篇 |
1985年 | 6篇 |
1984年 | 13篇 |
1983年 | 9篇 |
1982年 | 13篇 |
1981年 | 12篇 |
1980年 | 8篇 |
1979年 | 5篇 |
1977年 | 9篇 |
1976年 | 10篇 |
1975年 | 10篇 |
1974年 | 11篇 |
1973年 | 10篇 |
1972年 | 10篇 |
1971年 | 5篇 |
1970年 | 12篇 |
1969年 | 9篇 |
1968年 | 9篇 |
1967年 | 6篇 |
排序方式: 共有750条查询结果,搜索用时 250 毫秒
21.
22.
Fine mapping of the Autosomal Dominant Split Hand/Split Foot Locus on Chromosome 7, Band q21.3-q22.1 总被引:6,自引:3,他引:3
下载免费PDF全文
![点击此处可从《American journal of human genetics》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Stephen W. Scherer Parvoneh Poorkaj Todd Allen Julia Kim Dorrit Geshuri Mark Nunes Sylvia Soder Karen Stephens Roberta A. Pagon Michael A. Patton Mary Anne Berg Tim Donlon Horacio Rivera R. A. Pfeiffer Kenji Naritomi Helen Hughes Maurizio Genuardi Fiorella Gurrieri Giovanni Neri Everett Lovrein Ellen Magenis Lap-Chee Tsui James P. Evans 《American journal of human genetics》1994,55(1):12-20
Split hand/split foot (SHFD) is a human developmental defect characterized by missing digits, fusion of remaining digits, and a deep median cleft in the hands and feet. Cytogenetic studies of deletions and translocations associated with this disorder have indicated that an autosomal dominant split hand/split foot locus (gene SHFD1) maps to 7q21-q22. To characterize the SHFD1 locus, somatic cell hybrid lines were constructed from cytogenetically abnormal individuals with SHFD. Molecular analysis resulted in the localization of 93 DNA markers to one of 10 intervals surrounding the SHFD1 locus. The translocation breakpoints in four SHFD patients were encompassed by the smallest region of overlap among the SHFD-associated deletions. The order of DNA markers in the SHFD1 critical region has been defined as PON–D7S812–SHFD1–D7S811–ASNS. One DNA marker, D7S811, detected altered restriction enzyme fragments in three patients with translocations when examined by pulsed-field gel electro-phoresis (PFGE). These data map SHFD1, a gene that is crucial for human limb differentiation, to a small interval in the q21.3-q22.1 region of human chromosome 7. 相似文献
23.
Minimizing growth regulators in shoot culture of an endangered plant,Hackelia venusta (Boraginaceae)
John L. Edson Annette D. Leege-Brusven Richard L. Everett David L. Wenny 《In vitro cellular & developmental biology. Plant》1996,32(4):267-271
Summary
Hackelia venusta (Boraginaceae) is an endangered perennial herb endemic to the interior northwestern United States. Because of seed scarcity,
micropropagation (anex situ conservation strategy) could produce true-to-type plantlets suitable for reintroduction. We hypothesized that clones of predetermined
size could be rapidly produced by supplementing multiplication and rooting media with minimal levels of cytokinin and auxin.
Microshoots derived from shoot expants were cultured on Murashige and Skoog (1962) media supplemented with 1% (wt/vol) agar
and 0.0001 to 10 μM benzyladenine. Inverse regression estimates on 3 genotypes predicted that a target of 2.5 axillary microshoots per explant
would require a minimal level of 0.04±0.02 μM benzyladenine. Culture of 25 genotypes with 0.04 μM benzyladenine resulted in an average of 2.3±0.1 axillary microshoots per explant. Elongated microshoots were transferred
to media supplemented with 0.1 to 25 μM indoleacetic acid. Clones rooted from 36% to 100% success after 4 wk in 2.0 μM indoleacetic acid. Plantlets transplantedex vitro with three or more roots survived at 84% versus 46% of plantlets with fewer roots. Up to 84% of the plantlets survived in
a planting trial. The data suggest that shoot culture ofHackelia venusta, with minimal growth regulators, can produce axillary microshoots for reintroduction. 相似文献
24.
Everett Anderson Peter C. Hoppe Gloria S. Lee 《Molecular reproduction and development》1984,9(4):451-467
Karyotypic and light and electron microscopical analyses were made of spontaneous preimplantation mouse parthenotes from the LT/Sv inbred strain. It was found that the activated oocyte and developing embryos were diploid. We believe that diploidization is achieved by the oogonium undergoing a premeiotic mitosis without cytokinesis followed by two meiotic divisions, thus producing diploid parthenotes. The developmental events with respect to membrane specialization, such as junctional complexes, were similar to those observed in fertilized embryos. A unique feature of the developing parthenote was the failure of the mitochondria to change during the morula stage. The mitochondria retained a few irregularly oriented cristae rather than many transversely oriented ones observed in morulae developing from fertilized eggs. The significance of this observation is discussed. 相似文献
25.
26.
A polyacid-dependent dichrome has been devised which will differentiate epithelial from mesenchymal cells in young dividing primary cultures. Epithelial cells and colonies and nuclei are stained with metanil yellow, the stain is fixed and differentiated with phosphotungstic acid, and the mesenchymal elements are stained with toluidine blue. Several other dyes are tested for substitution in this method. Biebrich scarlet and aniline blue could be substituted for the metanil yellow; Bismarck brown T, Janus green B, crystal violet, and neutral red could be substituted for the basic dye. 相似文献
27.
28.
Jeremy R. Everett Donald W. Hughes Russell A. Bell Dirk Alkema Thomas Neilson Paul J. Romaniuk 《Biopolymers》1980,19(3):557-573
The variable-temperature proton nmr spectra of the oligoribonucleotides in the series CpApX and the series ApGpX, X = A, G, C, U, together with the parent dimers CpA and ApG have been measured. A complete analysis of all the nonexchangeable base proton resonances and ribose H-1′ proton resonances was made. The presence of trends in the shielding abilities of the various bases at both the nearest-neighbor and next-nearest-neighbor positions were identified. The observed shieldings could be used to predict the chemical shifts of protons in related systems. Based on the empirical results from ribodinucleoside monophosphates, the temperature-dependent behavior of the J1′2′ coupling constants of the triribonucleotides suggested that the compounds in the CpApX series stacked from the 5′-end to the 3′-end, while those in the ApGpX series stacked from the 3′-end to the 5′-end. 相似文献
29.
Equilibration of leucine between the plasma compartment and leucyl-tRNA in the heart, and turnover of cardiac myosin heavy chain. 总被引:7,自引:3,他引:4
下载免费PDF全文
![点击此处可从《The Biochemical journal》网站下载免费的PDF全文](/ch/ext_images/free.gif)
By 30min continuous infusion of [3H]leucine into rats, the specific radioactivities of plasma leucine and tissue-free and tRNA-bound leucine in heart were equal. The specific radioactivity of leucyl-tRNA in heart therefore follows a time course identical with that of plasma leucine soon after the start of infusion. The half-life of cardiac myosin heavy chain (5.5 days) was the same as that reported by other investigators who used the pulse-labelling protocol. 相似文献
30.
Henry R. Black Samuel O. Thier Philip F. Felig James F. Jekel Joseph Belsky James L. Bernene Everett Cooper Vincent A. De Luca Jr. Martin H. Floch Marvin Garrell Pasquale E. Perillie Robert L. Piscatelli George F. Thornton 《The Yale journal of biology and medicine》1977,50(6):637-644
In July, 1975, the Departments of Internal Medicine at the Yale University School of Medicine and eight community hospitals in southern and western Connecticut formed the Yale Affiliated Hospital Program (YAHP) in Internal Medicine. The YAHP provides a planned and focused program of continuing education for medical staff and housestaff at the affiliated hospitals. Six formats for the over 1,000 rounds, lectures, and conferences given annually are used. The members of the YAHP also cooperate in housestaff and faculty recruiting, evaluation of quality of care and evaluation of the process of continuing medical education itself. This report summarizes the organization, goals and future plans of the YAHP. 相似文献