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61.
Ilona Rousalova Sulagna Banerjee Veena Sangwan Kristen Evenson Joel A. McCauley Robert Kratzke Selwyn M. Vickers Ashok Saluja Jonathan D’Cunha 《PloS one》2013,8(10)
Background
Minnelide, a pro-drug of triptolide, has recently emerged as a potent anticancer agent. The precise mechanisms of its cytotoxic effects remain unclear.Methods
Cell viability was studied using CCK8 assay. Cell proliferation was measured real-time on cultured cells using Electric Cell Substrate Impedence Sensing (ECIS). Apoptosis was assayed by Caspase activity on cultured lung cancer cells and TUNEL staining on tissue sections. Expression of pro-survival and anti-apoptotic genes (HSP70, BIRC5, BIRC4, BIRC2, UACA, APAF-1) was estimated by qRTPCR. Effect of Minnelide on proliferative cells in the tissue was estimated by Ki-67 staining of animal tissue sections.Results
In this study, we investigated in vitro and in vivo antitumor effects of triptolide/Minnelide in non-small cell lung carcinoma (NSCLC). Triptolide/Minnelide exhibited anti-proliferative effects and induced apoptosis in NSCLC cell lines and NSCLC mouse models. Triptolide/Minnelide significantly down-regulated the expression of pro-survival and anti-apoptotic genes (HSP70, BIRC5, BIRC4, BIRC2, UACA) and up-regulated pro-apoptotic APAF-1 gene, in part, via attenuating the NF-κB signaling activity.Conclusion
In conclusion, our results provide supporting mechanistic evidence for Minnelide as a potential in NSCLC. 相似文献62.
KR N’Guessan BRD Aboua J Tison-Rosebery A Ouattara EP Kouamelan 《African Journal of Aquatic Science》2018,43(2):131-140
The ecology and taxonomy of the epilithic diatom flora of the Agnéby River, Ivory Coast were studied in 2012. Ten sites were investigated and diatoms were sampled on glass slides immersed for a period of 30 days during the wet and dry seasons. Physico-chemical parameters were measured at each site while sampling diatoms. Five taxa were largely dominant: Planothidium comperei CE Wetzel, N’Guessan and Tison-Rosebery, Eolimna minima (Grunow) Lange-Bertalot, Planothidium piaficum (JR Carter and Denny) CE Wetzel and Ector, Cocconeis schroederi Foged and Cocconeis scutellum var. parva (Grunow in Van Heurck) Cleve. Electrical conductivity, temperature, dissolved oxygen, nitrite and phosphorus were found to influence the distribution of taxa. 相似文献
63.
Biological samples, including cryopreserved sperm, are routinely X-rayed during air shipment. The goal was to investigate the impact of X-irradiation used for checked and carry-on luggage on bovine sperm chromatin integrity and postfertilization in vitro embryonic development. Frozen domestic bull sperm (Bos taurus) (n = 9 bulls) stored in a dry shipper (−160 °C) was screened by X-irradiation 0, 1, 2, and 3 times as either carry-on or checked luggage. Duplicate straws were thawed, and sperm were assessed for chromatin damage using the sperm chromatin structure assay (SCSA) and by postfertilization in vitro developmental competence of mature oocytes. Multiple exposure to X-rays did not significantly affect sperm chromatin integrity assessed by SCSA. There were lower proportions of oocytes cleaved (P = 0.07; 21.6 ± 3.1% vs. 29.4 ± 3.1%, 24.9 ± 3.1%, and 25.7 ± 3.3% for 3 vs. 0, 1, and 2 times, respectively; least-squares means ± SEM) and that developed to blastocysts (P = 0.06; 9.0 ± 1.7% vs. 13.8 ± 1.7%, 11.5 ± 1.7%, and 12.6 ± 1.9%, respectively) when fertilization was performed with sperm X-rayed 3 times using checked luggage irradiation; developmental competence (percentage cleaved embryos becoming blastocysts) was unaffected. There were no deleterious effects of other X-irradiation treatments on embryo development. We inferred that screening by X-irradiation may reduce the ability of sperm to activate oocyte cleavage after multiple exposures at the checked luggage dose. However, there was no evidence that competence of embryos to become blastocysts was reduced by X-irradiation (45.4 ± 5.7%, 40.4 ± 5.7%, 46.4 ± 6.1%, and 41.8 ± 5.7% for 0, 1, 2, and 3 doses, respectively), but potential long-term epigenetic effects are unknown. 相似文献
64.
Chandler C Bodenhamer-Davis E Holden JM Evenson T Bratton S 《Applied psychophysiology and biofeedback》2001,26(1):1-7
The purpose of this study was to explore whether biofeedback-assisted relaxation training could reduce stress-related symptoms and enhance personal well-being in a group of counselor trainees enrolled in a basic counseling skills course. Treatment participants received ten sessions of weekly biofeedback-assisted relaxation training, whereas the control participants received no intervention. The treatment group showed, significant improvements in several symptom areas measured by the Symptom Checklist 90-Revised: physical complaints (Somatization), personal inadequacy (Interpersonal Sensitivity), confused thinking or alienation or both (Psychoticism), and the overall number and severity of symptoms (Global Severity Index and Positive Symptom Total). The control participants showed no significant improvements in any symptom areas. Biofeedback-assisted relaxation was shown to be an effective stress-reducing intervention for counselor trainees, which resulted in a greater sense of personal well-being. 相似文献
65.
Boetel MA Fuller BW Chandler LD Tollefson JJ McManus BL Kadakia ND Evenson PD Mishra TP 《Journal of economic entomology》2005,98(6):1957-1968
Impacts of semiochemical-based insecticidal bait applications on beneficial arthropod groups common to field corn, Zea mays L., habitats were assessed in areawide-managed field sites in South Dakota and Iowa during 1997 and 1998. Slam, a commercial bait formulation comprised of 87% cucurbitacin and 13% carbaryl insecticide, was used for management of adult rootworm, Diabrotica spp., and controls consisted of cornfield habitats without bait applications. Effects on beneficial organisms were variable, and negative impacts were infrequent. Coccinellidae, Staphylinidae, and Anthocoridae were usually more abundant in bait-treated plots than in controls that received at-plant soil insecticides, especially by 4 wk postapplication. Carabid beetle activity also had increased in bait-treated corn by proportionally greater rates than in control plots at 4 wk postapplication in two of the four site by year combinations in this study. Impacts of semiochemical-based adulticide applications on Formicidae were not consistently negative or positive. The relative lack of consistent negative impacts on nontarget arthropods suggests that other biotic and abiotic factors leading to natural population fluxes may have more influence on these groups of beneficial organisms than applications of semiochemical-based bait containing carbaryl. Overall, it seems that areawide applications of these baits for managing rootworm populations in corn are not likely to impose deleterious effects on the nontarget faunal groups we surveyed, especially in comparison with the at-plant applications of soil insecticides used as experimental controls in this study. 相似文献
66.
N E Thompson M Razdan G Kuntsmann J M Aschenbach M L Evenson M S Bergdoll 《Applied and environmental microbiology》1986,51(5):885-890
Murine monoclonal antibodies reactive with at least one of the serological types of staphylococcal enterotoxin were examined for use in assay systems for the detection of enterotoxin at the level of 1.0 ng of enterotoxin per ml. An antibody sandwich enzyme-linked immunosorbent assay was devised for each toxin type by identifying an effective antibody pair. One antibody (the coating antibody) was coated onto a polystyrene plate and removed the enterotoxin from the test solution; the second antibody (the probing antibody) was conjugated to horseradish peroxidase and detected the captured toxin. Enterotoxins A and E could be detected in the same system by the use of cross-reacting monoclonal antibodies. All subtypes of enterotoxin C could be detected in one assay system. Two effective systems were described for each of types B and D. Each of these systems, when compared with the homologous enterotoxin-specific polyclonal rabbit antibody systems, was found to compare favorably. The monoclonal enzyme-linked immunosorbent assay systems for the detection of enterotoxins A and C2 were examined for a variety of food extracts; no abnormal interference could be detected from these extracts. The monoclonal antibody systems were also compared with the homologous enterotoxin-specific polyclonal serum for the detection of enterotoxin by the competitive radioimmunoassay (RIA). Single monoclonal antibodies generally did not perform as well in the RIA as did the homologous toxin-specific polyclonal serum. However, pools of monoclonal antibodies were prepared that approached the sensitivity and precision of the polyclonal system for the detection of each toxin by the RIA. 相似文献
67.
EDC3 phosphorylation regulates growth and invasion through controlling P‐body formation and dynamics
Jeremiah J Bearss Sathish KR Padi Neha Singh Marina CardoVila Jin H Song Ghassan Mouneimne Nikita Fernandes Yang Li Matthew R Harter Jaime MC Gard Anne E Cress Wolfgang Peti Andrew DL Nelson J Ross Buchan Andrew S Kraft Koichi Okumura 《EMBO reports》2021,22(4)
Regulation of mRNA stability and translation plays a critical role in determining protein abundance within cells. Processing bodies (P‐bodies) are critical regulators of these processes. Here, we report that the Pim1 and 3 protein kinases bind to the P‐body protein enhancer of mRNA decapping 3 (EDC3) and phosphorylate EDC3 on serine (S)161, thereby modifying P‐body assembly. EDC3 phosphorylation is highly elevated in many tumor types, is reduced upon treatment of cells with kinase inhibitors, and blocks the localization of EDC3 to P‐bodies. Prostate cancer cells harboring an EDC3 S161A mutation show markedly decreased growth, migration, and invasion in tissue culture and in xenograft models. Consistent with these phenotypic changes, the expression of integrin β1 and α6 mRNA and protein is reduced in these mutated cells. These results demonstrate that EDC3 phosphorylation regulates multiple cancer‐relevant functions and suggest that modulation of P‐body activity may represent a new paradigm for cancer treatment. 相似文献
68.
Samuel R. Rushforth Jack D. Brotherson Natha Fungladda William E. Evenson 《Hydrobiologia》1981,83(2):313-323
The relationships of diatom species to dissolved heavy metals in the streams of the Uintah Basin of Utah were studied through four seasons of 1977–1978. Niche center gradient analysis, cluster analysis and correlation analysis were performed.Achnanthes minutissima, Cyclotella meneghiniana, Cymbella minuta, Gomphonema parvulum, Navicula secreta var.apiculata, Nitzschia frustulum, Nitzschia frustulum var.perminuta, Nitzschia frustulum var.perpusilla, Nitzschia palea, andSynedra ulna appear to be indicator species of high or low heavy metal concentrations. Several other species also showed meaningful relationships to high or low heavy metal concentrations.Dept. of Physics and Astronomy, Brigham Young UniversityDept. of Physics and Astronomy, Brigham Young University 相似文献
69.
Shashank M Patil KR Maruthi Shrisha Naik Bajpe VM Vyshali S Sushmitha Chagalamari Akhila Ramith Ramu 《Bioinformation》2021,17(11):932
Treatment of SARS-CoV-2 targeting its RNA dependent RNA polymerase (RdRp) is of current interest. Remdesivir has been approved for the treatment of COVID-19 around the world. However, the drug has been linked with pharmacological limitations like adverse effects and reduced efficiency. Nevertheless, recent advancements have depicted molnupiravir as an effective therapeutic agent to target the SARS-CoV-2 RdRp. The drug has cleared both in vitro and in vivo screening. It is in phase-III clinical trial. Nonetheless, there are no data on themolecular binding interaction of molnupiravir with RdRp. Therefore, it is of interest to report the binding interaction of molnupiravir using molecular docking. It is also of interest to show its stability during interaction using molecular dynamics and binding free energy calculations along with drug likeliness and pharmacokinetic properties in comparison with remdesivir. 相似文献
70.
Vikalp Raj Kaustubh G. Naik Bairav S. Vishnugopi Ajeet Kumar Rana Andrew Scott Manning Smruti Rekha Mahapatra KR Varun Vipin Singh Abhineet Nigam Josefine D. McBrayer Partha P. Mukherjee Naga Phani B. Aetukuri David Mitlin 《Liver Transplantation》2024,14(15):2303062
This study illustrates how the microstructure of garnet solid-state electrolytes (SSE) affects the stress-state and dendrite growth. Tantalum-doped lithium lanthanum zirconium oxide (LLZTO, Li6.4La3Zr1.4Ta0.6O12) is synthesized by powder processing and sintering (AS), or with the incorporation of intermediate-stage high-energy milling (M). The M compact displays higher density (91.5% vs 82.5% of theoretical), and per quantitative stereology, lower average grain size (5.4 ± 2.6 vs 21.3 ± 11.1 µm) and lower AFM-derived RMS surface roughness contacting the Li metal (45 vs 161 nm). These differences enable symmetric M cells to electrochemically cycle at constant capacity (0.1 mAh cm−2) with enhanced critical current density (CCD) of 1.4 versus 0.3 mA cm−2. It is demonstrated that LLZTO grain size distribution and internal porosity critically affect electrical short-circuit failure, indicating the importance of electronic properties. Lithium dendrites propagate intergranularly through regions where LLZTO grains are smaller than the bulk average (7.4 ± 3.8 µm for AS in a symmetric cell, 3.1 ± 1.4 µm for M in a half-cell). Metal also accumulates in the otherwise empty pores of the sintered compact present along the dendrite path. Mechanistic modeling indicates that reaction and stress heterogeneities are interrelated, leading to current focusing and preferential plating at grain boundaries. 相似文献