全文获取类型
收费全文 | 3864篇 |
免费 | 344篇 |
国内免费 | 1篇 |
出版年
2022年 | 32篇 |
2021年 | 43篇 |
2020年 | 30篇 |
2019年 | 43篇 |
2018年 | 39篇 |
2017年 | 49篇 |
2016年 | 76篇 |
2015年 | 142篇 |
2014年 | 172篇 |
2013年 | 179篇 |
2012年 | 224篇 |
2011年 | 235篇 |
2010年 | 136篇 |
2009年 | 144篇 |
2008年 | 188篇 |
2007年 | 164篇 |
2006年 | 173篇 |
2005年 | 164篇 |
2004年 | 167篇 |
2003年 | 151篇 |
2002年 | 137篇 |
2001年 | 118篇 |
2000年 | 98篇 |
1999年 | 86篇 |
1998年 | 57篇 |
1997年 | 53篇 |
1996年 | 51篇 |
1995年 | 42篇 |
1994年 | 44篇 |
1993年 | 42篇 |
1992年 | 79篇 |
1991年 | 59篇 |
1990年 | 55篇 |
1989年 | 53篇 |
1988年 | 46篇 |
1987年 | 48篇 |
1986年 | 41篇 |
1985年 | 43篇 |
1984年 | 60篇 |
1983年 | 40篇 |
1982年 | 40篇 |
1981年 | 34篇 |
1980年 | 27篇 |
1979年 | 46篇 |
1978年 | 31篇 |
1977年 | 20篇 |
1976年 | 26篇 |
1975年 | 21篇 |
1974年 | 27篇 |
1972年 | 18篇 |
排序方式: 共有4209条查询结果,搜索用时 15 毫秒
71.
72.
Competition over mates takes many forms and has far-reaching consequences for many organisms. Recent work suggests that relative reproductive rates of males and females, sperm competition and quality variation among mates affect the strength of sexual selection. Song, other display, body size, visual ornaments and material resource offerings are often sexually selected. There is much empirical evidence of mate choice, and its evolution is clarified by mathematical models. Recent advances in theory also consider costs of choice, effects of deleterious mutations, fast and slow evolution of preferences and preferred traits, and simultaneous preferences for several traits. Contests over mates are important; so is sperm competition, scrambles, endurance rivalry, and coercion. The latter mechanisms have received less attention than mate choice. Sexual selection may explain puzzling aspects of plant pollination biology. 相似文献
73.
74.
Anders Kussak Barbro Andersson Kurt Andersson 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》1995,672(2)
A method for the determination of aflatoxins B1, B2, G1, G2, M1 and Q1 in human urine has been developed. The 10-ml urine samples were automatically cleaned up on immunoaffinity columns and analysed by high-performance liquid chromatography (HPLC), including post-column derivatization with bromine and fluorescence detection. Average aflatoxin recoveries were: B1 103%, B2 106%, G1 98% and G2 96% in the range 6.8–73 pg/ml of urine and M1 103% and Q1 100% in the range 18–97 pg/ml of urine. The relative standard deviations were all between 1% and 21%. The determination limits of aflatoxins in urine were 6.8 pg/ml for B1, B2, G1 and G2 and 18 pg/ml for M1 and Q1. 相似文献
75.
A Primary Linkage Map of the Porcine Genome Reveals a Low Rate of Genetic Recombination 总被引:12,自引:0,他引:12 下载免费PDF全文
H. Ellegren B. P. Chowdhary M. Johansson L. Marklund M. Fredholm I. Gustavsson L. Andersson 《Genetics》1994,137(4):1089-1100
A comprehensive genetic linkage map of the porcine genome has been developed by typing 128 genetic markers in a cross between the European Wild Boar and a domestic breed (Large White). The marker set includes 68 polymerase chain reaction-formatted microsatellites, 60 anchored reference markers informative for comparative mapping and 47 markers which have been physically assigned by in situ hybridization. Novel multipoint assignments are provided for 54 of the markers. The map covers about 1800 cM, and the average spacing between markers is 11 cM. We used the map data to estimate the genome size in pigs, thereby addressing the total recombination distance in a third mammalian species. A sex-average genome length of 1873 +/- 139 cM was obtained by comparing the recombinational and physical distances in defined regions of the genome. This is strikingly different from the length of the human genome (3800-4000 cM) and is more similar to the mouse estimate (1600 cM). The recombination rate in females was significantly higher than in males. 相似文献
76.
Nilufar Nahar Balai K. Hazra M. Mosihuzzaman M. Mahbubur RahmanRolf Andersson 《Carbohydrate polymers》1994,24(4):277-280
The structure of an acidic polysaccharide isolated from Abroma augusta root bark was determined by sugar and methylation analyses and high resolution 1H- and 13C-NMR spectroscopy. The main chain of the polysaccharide was composed of 1,2-linked -
-rhamnopyranose and 1,4- or 1,3-linked -
-galacturonic acid residues. The terminal β-
-glucuronic acid residue was attached to the 3- and/or 4-position of the -
-galacturonic acid residue. 相似文献
77.
M. Johansson H. Ellegren L. Marklund W. Coppieters L. Andersson 《Mammalian genome》1994,5(12):785-790
Linkage maps of porcine Chromosomes (Chrs) 3, 6, and 9, based on 31 polymorphic markers, are reported. The markers include 14 microsatellites, 12 RFLPs, three protein polymorphisms, and two blood group loci. The genetic interpretations of 11 RFLPs are documented. The markers were scored in a three-generation Wild Boar/Large White pedigree, and genetic maps were constructed on the basis of two-point and multi-point linkage analysis. Altogether the maps span a genetic distance of 216 cM, and previous physical assignments indicate that the linkage groups cover major parts of the three chromosomes. Significant differences in recombination rates between the sexes were observed for all three chromosomes. The recombination rate on the q arm of Chr 6 was markedly low. Sixteen loci are informative with regard to comparative mapping, that is, they have previously been mapped in the human and/or mouse genomes. 相似文献
78.
Michael R. Kanost Melissa K. Zepp Noma E. Ladendorff Laura A. Andersson 《Archives of insect biochemistry and physiology》1994,27(2):123-136
A protein that inhibits hemocyte aggregation has been isolated from hemolymph of Manduca sexta larvae and named hemocyte aggregation inhibitor protein (HAIP). HAIP has a Mr = 50,000, pI = 8.5, and contains 7% carbohydrate. It is present at 230 ± 20 μg/ml in hemolymph of day 3 fifth instar larvae. Antibodies to HAIP do not cross-react with M. sexta hemolin, which is similar in size and charge and also inhibits hemocyte aggregation. HAIP and hemolin have some similarity in amino acid composition and NH2-terminal sequence, but are different in overall secondary structure, as determined by CD spectroscopy. The concentration of HAIP in hemolymph is not affected by injection of larvae with bacteria. A protein of approximately 50,000 daltons that reacts with antibody to M. sexta HAIP is present in hemolymph of Bombyx mori, Heliothis zea, and Galleria mellonella. Although the function of HAIP in vivo is not yet clear, it may have a role in modulating adhesion of hemocytes during defensive responses. © 1994 Wiley-Liss, Inc. 相似文献
79.
Mario Herrera-Marschitz C. Fabián Loidl Zhi-Bing You Kurt Andersson Rodolfo Silveira William T. O'Connor Michel Goiny 《Molecular neurobiology》1994,9(1-3):171-182
The neurocircuitries of the basal ganglia are studied with in vivo microdialysis, with special consideration to dopamine transmission and its interaction with other neurotransmitter systems. The aim is to develop experimental models to study the pathophysiology and therapy of neurodegenerative disorders of the basal ganglia, as well as to develop models to study the short- and long-term consequences of perinatal asphyctic lesions. A main goal of these studies is to find and to characterize new treatments for these disorders. 相似文献
80.
Gary C. du Moulin Zorina Pitkin Yuan-Jin Shen Evelyn Conti Jean Ko Stewart Carla Charles Dylan Hamilton 《Cytotechnology》1994,15(1-3):365-372
Somatic cell and gene therapy involve the application of biological technologies to an individual patient through the use of living cells which provide a therapeutic benefit (Aliski, 1991). Various forms of cellular and gene therapies are being developed and evaluated in an increasing number of clinical trials for congential and acquired disorders. The potential and progress of these therapeutic applications have resulted in an increasing effort by the Food and Drug Administration (FDA) to develop the regulatory framework under which these therapeutic approaches would insure safety and efficacy, the primary mandate of the FDA.Over five years ago Cellcor began to define the parameters, specifications, and conditions relevant to a Quality Assurance/Quality Control (QA/QC) program that has evolved to insure safety and maximize the efficacy of applications of the company'sex vivo technology, autolymphocyte therapy. Autolymphocyte therapy is an outpatient form of somatic cell immunotherapy based upon the infusion of T cells that have been activatedex vivo using a combination of previously generated autologous cytokines and an anti-CD3 monoclonal antibody.We have been able to demonstrate the feasibility for the safe, controlled, and consistent preparation and delivery of a cellular therapy by application of relevant GMP regulations. This presentation reviews aspects of this program and chronicles our experience which at present amounts to over 4400 infusions for over 700 patients. This program provides a high degree of assurance that a cellular therapy program can be carried out in a multisite mode involving hundreds of patients through the strict adherence to cGMP as set forth in existing regulations. It would be prudent that developers of cellular andex vivo gene therapies establish a similar cell processing and QA/QC infrastructure at an early developmental stage to optimize safety and reproducibility and facilitate regulatory review. 相似文献