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排序方式: 共有196条查询结果,搜索用时 31 毫秒
111.
Costanzo E Gulino M Lanzanò L Musumeci F Scordino A Tudisco S Sui L 《European biophysics journal : EBJ》2008,37(2):235-238
Time resolved spectral components of delayed luminescence (DL) from single dry soybean seeds were measured using a device
with single photon sensitivity. The seeds were aged by a thermal treatment to change their viability. A correlation was observed
between the seeds viability and some DL parameters, i.e. the total number of photons emitted and the relative decay probability
of excited states. This relevant result confirms the close connection between the state of biological systems and their DL,
and it can allow the development of a quick selection technique for single dry seeds, a goal impossible up today. 相似文献
112.
Ballantine David L. Gerwick William H. Velez Sylvia M. Alexander Evelina Guevara Pablo 《Hydrobiologia》1987,151(1):463-469
113.
Edward Ryder Diane Gleeson Debarati Sethi Sapna Vyas Evelina Miklejewska Priya Dalvi Bishoy Habib Ross Cook Matthew Hardy Kalpesh Jhaveri Joanna Bottomley Hannah Wardle-Jones James N. Bussell Richard Houghton Jennifer Salisbury William C. Skarnes Ramiro Ramirez-Solis 《Mammalian genome》2013,24(7-8):286-294
The Sanger Mouse Genetics Project generates knockout mice strains using the EUCOMM/KOMP-CSD embryonic stem (ES) cell collection and characterizes the consequences of the mutations using a high-throughput primary phenotyping screen. Upon achieving germline transmission, new strains are subject to a panel of quality control (QC) PCR- and qPCR-based assays to confirm the correct targeting, cassette structure, and the presence of the 3′ LoxP site (required for the potential conditionality of the allele). We report that over 86 % of the 731 strains studied showed the correct targeting and cassette structure, of which 97 % retained the 3′ LoxP site. We discuss the characteristics of the lines that failed QC and postulate that the majority of these may be due to mixed ES cell populations which were not detectable with the original screening techniques employed when creating the ES cell resource. 相似文献
114.
Gemma Moncunill Alfredo Mayor Alfons Jiménez Augusto Nhabomba Laura Puyol Maria N. Manaca Diana Barrios Pau Cisteró Caterina Guinovart Ruth Aguilar Azucena Bardají María-Jesús Pinazo Evelina Angov Sheetij Dutta Chetan E. Chitnis José Mu?oz Joaquim Gascón Carlota Doba?o 《PloS one》2013,8(2)
Age- and exposure-dependent immune responses during a malaria episode may be key to understanding the role of these factors in the acquisition of immunity to malaria. Plasma/serum samples collected from naïve Mozambican children (n = 48), European adults (naïve travelers, n = 22; expatriates with few prior malaria exposures, n = 15) and Mozambican adults with long-life malaria exposure (n = 99) during and after a malaria episode were analyzed for IgG against merozoite proteins by Luminex and against infected erythrocytes by flow cytometry. Cytokines and chemokines were analyzed in plasmas/sera by suspension array technology. No differences were detected between children and adults with a primary infection, with the exception of higher IgG levels against 3D7 MSP-142 (P = 0.030) and a P. falciparum isolate (P = 0.002), as well as higher IL-12 (P = 0.020) in children compared to other groups. Compared to malaria-exposed adults, children, travelers and expatriates had higher concentrations of IFN-γ (P≤0.0090), IL-2 (P≤0.0379) and IL-8 (P≤0.0233). Children also had higher IL-12 (P = 0.0001), IL-4 (P = 0.003), IL-1β (P = 0.024) and TNF (P = 0.006) levels compared to malaria-exposed adults. Although IL-12 was elevated in children, overall the data do not support a role of age in immune responses to a first malaria episode. A TH1/pro-inflammatory response was the hallmark of non-immune subjects. 相似文献
115.
Valueva OA Zdorovenko EL Kachala VV Varbanets LD Arbatsky NP Shubchynskyy VV Shashkov AS Knirel YA 《Carbohydrate research》2011,346(1):146-149
The following structure of the O-polysaccharide of Pragia fontium 27480 was elucidated by sugar analysis, including determination of the absolute configurations of the monosaccharides, and Smith degradation along with 1D and 2D (1)H and (13)C NMR spectroscopy: →4)-β-d-ManpNAc3NAcA-(1→2)-α-l-Rhap-(1→3)-β-l-Rhap-(1→4)-α-d-GlcpNAc-(1→ where ManNAc3NAcA stands for 2,3-diacetamido-2,3-dideoxymannuronic acid. 相似文献
116.
Edward Ryder Brendan Doe Diane Gleeson Richard Houghton Priya Dalvi Evelyn Grau Bishoy Habib Evelina Miklejewska Stuart Newman Debarati Sethi Caroline Sinclair Sapna Vyas Hannah Wardle-Jones Joanna Bottomley James Bussell Antonella Galli Jennifer Salisbury Ramiro Ramirez-Solis 《Transgenic research》2014,23(1):177-185
We describe here use of a cell-permeable Cre to efficiently convert the EUCOMM/KOMP-CSD tm1a allele to the tm1b form in preimplantation mouse embryos in a high-throughput manner, consistent with the requirements of the International Mouse Phenotyping Consortium-affiliated NIH KOMP2 project. This method results in rapid allele conversion and minimizes the use of experimental animals when compared to conventional Cre transgenic mouse breeding, resulting in a significant reduction in costs and time with increased welfare benefits. 相似文献
117.
Gamberoni G Lamma E Lodo G Marchesini J Mascellani N Rossi S Storari S Tagliavini L Volinia S 《Bioinformatics (Oxford, England)》2007,23(20):2725-2732
MOTIVATION: Microarray and other genome-wide technologies allow a global view of gene expression that can be used in several ways and whose potential has not been yet fully discovered. Functional insight into expression profiles is routinely obtained by using gene ontology terms associated to the cellular genes. In this article, we deal with functional data mining from expression profiles, proposing a novel approach that studies the correlations between genes and their relations to Gene Ontology (GO). We implemented this approach in a public web-based application named Fun&Co. By using Fun&Co, the user dissects in a pair-wise manner gene expression patterns and links correlated pairs to gene ontology terms. The proof of principle for our study was accomplished by dissecting molecular pathways in muscles. In particular, we identified specific cellular pathways by comparing the three different types of muscle in a pairwise fashion. In fact, we were interested in the specific molecular mechanisms regulating the cardiovascular system (cardiomyocytes and smooth muscle cells). RESULTS: We applied here Fun&Co to the molecular study of cardiovascular system and the identification of the specific molecular pathways in heart, skeletal and smooth muscles (using 317 microarrays) and to reveal functional differences between the three different kinds of muscle cells. AVAILABILITY: Application is online at http://tommy.unife.it. SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online. 相似文献
118.
Yeast mutants lacking telomerase are able to elongate their telomeres through processes involving homologous recombination. In this study, we investigated telomeric recombination in several mutants that normally maintain very short telomeres due to the presence of a partially functional telomerase. The abnormal colony morphology present in some mutants was correlated with especially short average telomere length and with a requirement for RAD52 for indefinite growth. Better-growing derivatives of some of the mutants were occasionally observed and were found to have substantially elongated telomeres. These telomeres were composed of alternating patterns of mutationally tagged telomeric repeats and wild-type repeats, an outcome consistent with amplification occurring via recombination rather than telomerase. Our results suggest that recombination at telomeres can produce two distinct outcomes in the mutants we studied. In occasional cells, recombination generates substantially longer telomeres, apparently through the roll-and-spread mechanism. However, in most cells, recombination appears limited to helping to maintain very short telomeres. The latter outcome likely represents a simplified form of recombinational telomere maintenance that is independent of the generation and copying of telomeric circles. 相似文献
119.
Duclos S Clavarino G Rousserie G Goyette G Boulais J Camossetto V Gatti E LaBoissière S Pierre P Desjardins M 《Proteomics》2011,11(5):854-864
The essential roles of the endovacuolar system in health and disease call for the development of new tools allowing a better understanding of the complex molecular machinery involved in endocytic processes. We took advantage of the floating properties of small latex beads (sLB) on a discontinuous sucrose gradient to isolate highly purified endosomes following internalization of small latex beads in J774 macrophages and bone marrow-derived dendritic cells (DC). We particularly focused on the isolation of macrophages early endosomes and late endosomes/lysosomes (LE/LYS) as well as the isolation of LE/LYS from immature and lipopolysaccharide-activated (mature) DC. We subsequently performed a comparative analysis of their respective protein contents by MS. As expected, proteins already known to localize to the early endosomes were enriched in the earliest fraction of J774 endosomes, while proteins known to accumulate later in the process, such as hydrolases, were significantly enriched in the LE/LYS preparations. We next compared the LE/LYS protein contents of immature DC and mature DC, which are known to undergo massive reorganization leading to potent immune activation. The differences between the protein contents of endocytic organelles from macrophages and DC were underlined by focusing on previously poorly characterized biochemical pathways, which could have an unexpected but important role in the endosomal functions of these highly relevant immune cell types. 相似文献
120.
Campo JJ Dobaño C Sacarlal J Guinovart C Mayor A Angov E Dutta S Chitnis C Macete E Aponte JJ Alonso PL 《PloS one》2011,6(10):e25779