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101.
Cristina Peggion Fernando Formaggio Marco Crisma Raquel F Epand Richard M Epand Claudio Toniolo 《Journal of peptide science》2003,9(11-12):679-689
Lipopeptaibols are members of a novel family of naturally occurring, short peptides with antimicrobial activity, characterized by a lipophilic acyl chain at the N-terminus, a high content of turn/helix inducing alpha-aminoisobutyric acid and a 1,2-amino alcohol at the C-terminus. Using solution methods, the prototypical lipopeptaibol trichogin GA IV and a large series of appropriately designed analogues were synthesized, which allow: (i) determination of the minimal lipid chain and peptide main-chain lengths for the onset of membrane activity, and (ii) exploitation of a number of physico-chemical techniques aimed at assessing the trichogin preferred conformation under a variety of conditions and at investigating its mechanism of interaction with the phospholipid membranes. 相似文献
102.
Location and aggregation of the spin-labeled peptide trichogin GA IV in a phospholipid membrane as revealed by pulsed EPR 下载免费PDF全文
Salnikov ES Erilov DA Milov AD Tsvetkov YD Peggion C Formaggio F Toniolo C Raap J Dzuba SA 《Biophysical journal》2006,91(4):1532-1540
The lipopeptaibol trichogin GA IV is a 10 amino acid-long residue and alpha-aminoisobutyric acid-rich antibiotic peptide of fungal origin. TOAC (2,2,6,6-tetramethylpiperidine-1-oxyl-4-amino-4-carboxylic acid) spin-labeled analogs of this membrane active peptide were investigated in hydrated bilayers of dipalmitoylphosphatidylcholine by electron spin echo envelope modulation (ESEEM) spectroscopy and pulsed electron-electron double resonance (PELDOR). Since, the ESEEM of the spin label appears to be strongly dependent on the presence of water molecules penetrated into the membrane, this phenomenon was used to study the location of this peptide in the membrane. This was achieved by comparing the ESEEM spectra for peptides labeled at different positions along the amino acid sequence with spectra known for lipids with spin labels at different positions along the hydrocarbon chain. To increase the ESEEM amplitude and to distinguish the hydrogen nuclei of water from lipid protons, membranes were hydrated with deuterated water. The PELDOR spectroscopy technique was chosen to study peptide aggregation and to determine the mutual distance distribution of the spin-labeled peptides in the membrane. The location of the peptide in the membrane and its aggregation state were found to be dependent on the peptide concentration. At a low peptide/lipid molar ratio (less than 1:100) the nonaggregated peptide chain of the trichogin molecules lie parallel to the membrane surface, with TOAC at the 4th residue located near the 9th-11th carbon positions of the sn-2 lipid chain. Increasing this ratio up to 1:20 leads to a change in peptide orientation, with the N-terminus of the peptide buried deeper into membrane. Under these conditions peptide aggregates are formed with a mean aggregate number of about N = 2. The aggregates are further characterized by a broad range of intermolecular distances (1.5-4 nm) between the labels at the N-terminal residues. The major population exhibits a distance of approximately 2.5 nm, which is of the same order as the length of the helical peptide. We suggest that the constituting monomers of the dimer are antiparallel oriented. 相似文献
103.
4‐Cyano‐α‐methyl‐l‐phenylalanine as a Spectroscopic Marker for the Investigation of PeptaibioticMembrane Interactions 下载免费PDF全文
Marta De Zotti Sara Bobone Annalisa Bortolotti Edoardo Longo Barbara Biondi Cristina Peggion Fernando Formaggio Claudio Toniolo Andrea Dalla Bona Bernard Kaptein Lorenzo Stella 《化学与生物多样性》2015,12(4):513-527
Two analogs of the ten‐amino acid residue, membrane‐active lipopeptaibiotic trichogin GA IV, mono‐labeled with 4‐cyano‐α‐methyl‐L ‐phenylalanine, a potentially useful fluorescence and IR absorption probe of the local microenvironment, were synthesized by the solid‐phase methodology and conformationally characterized. The single modification was incorporated either at the N‐terminus (position 1) or near the C‐terminus (position 8) of the peptide main chain. In both cases, the replaced amino acid was the equally helicogenic α‐aminoisobutyric acid (Aib) residue. We performed a solution conformational analysis by use of FT‐IR absorption, CD, and 2D‐NMR spectroscopies. The results indicate that both labeled analogs essentially maintain the overall helical propensity of the naturally occurring lipopeptaibiotic. Peptide? membrane interactions were assessed by fluorescence and ATR‐IR absorption techniques. Analogies and differences between the two peptides were highlighted. Taken together, our data confirm literature results that some of the spectroscopic parameters of the 4‐cyanobenzyl chromophore are sensitive markers of the local microenvironment. 相似文献
104.
F Marchiori G Borin B Filippi V Moretto G M Bonora C Toniolo 《International journal of peptide and protein research》1979,14(2):143-152
The synthesis of peptides containing blocks of arginyl residues is proposed through amidination of the corresponding ornithyl analogs. In order to test this strategy the ornithyl analog of the C-terminal sequence 52--65 of galline was synthesized by the conventional method. The amidination reaction, performed on fragments of different length and ornithyl-residue content, quantitatively converts ornithines into arginines. The strategy proposed may represent a powerful tool for the synthesis of protamines and other basic proteins. 相似文献
105.
C Toniolo G Valle G M Bonora F Lelj V Barone F Fraternali G Callet J Wagnon D Nisato 《International journal of peptide and protein research》1987,30(5):583-595
The conformational preferences and self-association modes of the two diastereomeric N-acetyl, methylamides of 3-hydroxy, 4-amino, 6-methylheptanoic acid (statine) with (R, S) and (S, S) configurations at the 3-hydroxy and 4-amino carbons, respectively, have been determined in solution as well as in the solid state by infrared absorption, 1H nuclear magnetic resonance, and X-ray diffraction. Conformational energy computations have also been performed in parallel. In the crystal state, the change in chirality of the hydroxyl group induces different intermolecular H-bonding schemes in the (R, S) isomer compared to the two structurally distinct molecules in the asymmetric unit of the (S, S) isomer. Different propensities to self-aggregate are seen in solvents of low polarity. In solvents of high polarity, however, the molecules of both isomers are largely solvated, while still keeping some local conformational restriction. Conformational energy computations indicate that in vacuo the two diastereomers exhibit different flexibility, and a preferred conformation with a different type of intramolecular H-bond. 相似文献
106.
Solid-state NMR and FT-IR absorption spectroscopy are employed to study the molecular properties of 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) lipids as a function of trichogin-OMe content, a membrane-active analogue of the peptaibol trichogin GA IV. Variable-temperature NMR studies are performed, comprising (13)C-, (31)P-, and (14)N-NMR line-shape and relaxation experiments, to provide information about the mobility and ordering of the phospholipid head group and the acyl-chain region in the absence and presence of trichogin-OMe. Likewise, variable-temperature FT-IR-absorption studies are performed, and the conformation-sensitive CH(2) stretching bands are analyzed to examine the conformational state of the acyl chain. At lower trichogin-OMe concentrations, the peptide exhibits no remarkable influence on the dynamics and ordering features of the phospholipid molecules. It is concluded that, in this case, trichogin-OMe is embedded in the lipid bilayer, with its helix axis laying parallel to the bilayer plane, the more hydrophobic part pointing towards the inner part of the bilayer ('carpet-like' superstructure). The lipid dynamics are probed by rotating-frame spin-lattice-relaxation (T(1rho)) experiments for the (13)C and (31)P nuclei, which are assumed to be dominated by collective-order fluctuations. Variation of T(1rho) with sample composition is attributed to changes of the membrane stiffness. For the sample with the highest lipid/peptide (L/P) molar ratio, i.e., L/P 5 : 1, phase separation as a result of membrane disruption occurs. In this case, a second spectroscopic component can be separated in the (31)P-NMR spectra. In addition, the (motionally averaged) magnetic interactions are greatly reduced, the actual values differing for both components. The second spectroscopic component refers to membrane components with high trichogin-OMe concentration and to strong lipid-trichogin-OMe interactions, as reflected by significant changes of the head-group orientation (toroidal model). At the same time, DMPC molecules exist with minor lipid-trichogin-OMe interactions, most probably in smaller liposomes, trichogin-OMe being embedded in a 'carpet-like' manner. Moreover, lipid ordering is generally reduced for the highly concentrated sample, which may result from fast lateral lipid motion along the curved bilayer surface. 相似文献
107.
Total syntheses in solution of a set of four selected analogues of the 19-mer component F50/5 of alamethicin, the most extensively studied among the channel-former peptaibol antibiotics, are planned and reported. All analogues bear three Glu(OMe) residues, replacing the Gln residues at positions 7, 18, and 19 of the naturally occurring compound. Three analogues are mono-labelled with the free-radical-containing amino acid residue TOAC at the strategic positions 1, 8, or 16. The fourth analogue is bis-labelled with the same EPR-active residue at both positions 1 and 16. In the native sequence, all of the positions where TOAC replacements have been introduced are characterized by residues of Aib, the prototype of the class of helicogenic C(alpha)-tetrasubstituted alpha-amino acids. All of the TOAC analogues synthesized exhibit significant membrane-modifying properties. 相似文献
108.
Platyscytus decempunctatus Carvalho is a small mirid living throughout its life cicle plant Solanum cernuum Vell. (Solanaceae). The internal morphology of P. decempunctatus was studied and found that the female has a telotrophic ovary with three ovarioles in the right ovary and five in the left one. In the male reproductive trait there is a well developed tubular accessory gland. The phytophagous feeding habitat of P. decempunctatus was confirmed by the observations of chlorophyll pigments in the gut of all dissected specimens. The tubular salivary glands and the Malpighian tubules were also discussed. 相似文献
109.
Licini G Bonchio M Broxterman QB Kaptein B Moretto A Toniolo C Scrimin P 《Biopolymers》2006,84(1):97-104
C(alpha)-tetrasubstituted alpha-amino acids constitute a powerful tool for controlling the conformation of short peptide sequences. Chiral peptides may be used in stereoselective reactions both for asymmetric induction and in kinetic resolution. By reviewing recent data from our own laboratories and by presenting new results on the stereoselective oxidation of chalcone to the corresponding oxide, this contribution shows that the control of peptide conformation is a critical issue in order to achieve successful stereoselective catalysis. 相似文献
110.
Relevant parameters and stereochemical consequences of helices [alpha-helix, 3(10)-helix, beta-bend ribbon spiral, gamma-helix, 2.0(5)-helix, poly(Pro)(n) type-I and -II helices, and collagen triple helix] of peptides based on alpha-amino acids for use as templates in various branches of chemistry are briefly discussed. 相似文献