全文获取类型
收费全文 | 12851篇 |
免费 | 897篇 |
国内免费 | 9篇 |
出版年
2024年 | 13篇 |
2023年 | 44篇 |
2022年 | 137篇 |
2021年 | 249篇 |
2020年 | 188篇 |
2019年 | 227篇 |
2018年 | 376篇 |
2017年 | 305篇 |
2016年 | 499篇 |
2015年 | 793篇 |
2014年 | 897篇 |
2013年 | 920篇 |
2012年 | 1220篇 |
2011年 | 1101篇 |
2010年 | 713篇 |
2009年 | 617篇 |
2008年 | 879篇 |
2007年 | 779篇 |
2006年 | 643篇 |
2005年 | 601篇 |
2004年 | 553篇 |
2003年 | 492篇 |
2002年 | 393篇 |
2001年 | 230篇 |
2000年 | 212篇 |
1999年 | 189篇 |
1998年 | 80篇 |
1997年 | 60篇 |
1996年 | 40篇 |
1995年 | 36篇 |
1994年 | 30篇 |
1993年 | 22篇 |
1992年 | 34篇 |
1991年 | 29篇 |
1990年 | 19篇 |
1989年 | 18篇 |
1988年 | 13篇 |
1987年 | 7篇 |
1986年 | 10篇 |
1985年 | 5篇 |
1984年 | 8篇 |
1983年 | 5篇 |
1982年 | 6篇 |
1981年 | 5篇 |
1980年 | 5篇 |
1977年 | 5篇 |
1976年 | 6篇 |
1974年 | 8篇 |
1967年 | 5篇 |
1966年 | 5篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
91.
Emmanuel Margolin Youngjun J. Oh Matthew Verbeek Jason Naude Daniel Ponndorf Yulia Alexandrovna Meshcheriakova Hadrien Peyret Michiel T. van Diepen Ros Chapman Ann E. Meyers George Peter Lomonossoff Nobuyuki Matoba Anna‐Lise Williamson Edward P. Rybicki 《Plant biotechnology journal》2020,18(10):2109-2117
92.
93.
Mayur Nimbadas Devare Yeong Hyeock Kim Joohye Jung Woo Kyu Kang Ki‐Sun Kwon Jeong‐Yoon Kim 《Aging cell》2020,19(6)
Glucose controls the phosphorylation of silent information regulator 2 (Sir2), a NAD+‐dependent protein deacetylase, which regulates the expression of the ATP‐dependent proton pump Pma1 and replicative lifespan (RLS) in yeast. TORC1 signaling, which is a central regulator of cell growth and lifespan, is regulated by glucose as well as nitrogen sources. In this study, we demonstrate that TORC1 signaling controls Sir2 phosphorylation through casein kinase 2 (CK2) to regulate PMA1 expression and cytoplasmic pH (pHc) in yeast. Inhibition of TORC1 signaling by either TOR1 deletion or rapamycin treatment decreased PMA1 expression, pHc, and vacuolar pH, whereas activation of TORC1 signaling by expressing constitutively active GTR1 (GTR1Q65L) resulted in the opposite phenotypes. Deletion of SIR2 or expression of a phospho‐mutant form of SIR2 increased PMA1 expression, pHc, and vacuolar pH in the tor1Δ mutant, suggesting a functional interaction between Sir2 and TORC1 signaling. Furthermore, deletion of TOR1 or KNS1 encoding a LAMMER kinase decreased the phosphorylation level of Sir2, suggesting that TORC1 signaling controls Sir2 phosphorylation. It was also found that Sit4, a protein phosphatase 2A (PP2A)‐like phosphatase, and Kns1 are required for TORC1 signaling to regulate PMA1 expression and that TORC1 signaling and the cyclic AMP (cAMP)/protein kinase A (PKA) pathway converge on CK2 to regulate PMA1 expression through Sir2. Taken together, these findings suggest that TORC1 signaling regulates PMA1 expression and pHc through the CK2–Sir2 axis, which is also controlled by cAMP/PKA signaling in yeast. 相似文献
94.
95.
Jisub Hwang Chang-Sook Jeong Chang Woo Lee Seung Chul Shin Han-Woo Kim Sung Gu Lee Ui Joung Youn Chang Sup Lee Tae-Jin Oh Hak Jun Kim Hyun Park Hyun Ho Park Jun Hyuck Lee 《Journal of microbiology (Seoul, Korea)》2020,58(7):606-613
Crystal structures of enoyl-coenzyme A (CoA) isomerase from Bosea sp. PAMC 26642 (BoECI) and enoyl-CoA hydratase from Hymenobacter sp. PAMC 26628 (HyECH) were determined at 2.35 and 2.70 Å resolution, respectively. BoECI and HyECH are members of the crotonase superfamily and are enzymes known to be involved in fatty acid degradation. Structurally, these enzymes are highly similar except for the orientation of their C-terminal helix domain. Analytical ultracentrifugation was performed to determine the oligomerization states of BoECI and HyECH revealing they exist as trimers in solution. However, their putative ligand-binding sites and active site residue compositions are dissimilar. Comparative sequence and structural analysis revealed that the active site of BoECI had one glutamate residue (Glu135), this site is occupied by an aspartate in some ECIs, and the active sites of HyECH had two highly conserved glutamate residues (Glu118 and Glu138). Moreover, HyECH possesses a salt bridge interaction between Glu98 and Arg152 near the active site. This interaction may allow the catalytic Glu118 residue to have a specific conformation for the ECH enzyme reaction. This salt bridge interaction is highly conserved in known bacterial ECH structures and ECI enzymes do not have this type of interaction. Collectively, our comparative sequential and structural studies have provided useful information to distinguish and classify two similar bacterial crotonase superfamily enzymes. 相似文献
96.
97.
Woo Ryung Kim Eun Gyung Park Kyung-Won Kang Sang-Myeong Lee Bumseok Kim Heui-Soo Kim 《Molecules and cells》2020,43(11):953
Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is an infectious disease with multiple severe symptoms, such as fever over 37.5°C, cough, dyspnea, and pneumonia. In our research, microRNAs (miRNAs) binding to the genome sequences of severe acute respiratory syndrome coronavirus (SARS-CoV), Middle East respiratory-related coronavirus (MERS-CoV), and SARS-CoV-2 were identified by bioinformatic tools. Five miRNAs (hsa-miR-15a-5p, hsa-miR-15b-5p, hsa-miR-195-5p, hsa-miR-16-5p, and hsa-miR-196a-1-3p) were found to commonly bind to SARS-CoV, MERS-CoV, and SARS-CoV-2. We also identified miRNAs that bind to receptor proteins, such as ACE2, ADAM17, and TMPRSS2, which are important for understanding the infection mechanism of SARS-CoV-2. The expression patterns of those miRNAs were examined in hamster lung samples infected by SARS-CoV-2. Five miRNAs (hsa-miR-15b-5p, hsa-miR-195-5p, hsa-miR-221-3p, hsa-miR-140-3p, and hsa-miR-422a) showed differential expression patterns in lung tissues before and after infection. Especially, hsa-miR-15b-5p and hsa-miR-195-5p showed a large difference in expression, indicating that they may potentially be diagnostic biomarkers for SARS-CoV-2 infection. 相似文献
98.
99.
Jinho Yoon Minkyu Shin Joungpyo Lim Dong Yeon Kim Taek Lee Jeong‐Woo Choi 《Biotechnology journal》2020,15(6)
Biomolecules, especially proteins and nucleic acids, have been widely studied to develop biochips for various applications in scientific fields ranging from bioelectronics to stem cell research. However, restrictions exist due to the inherent characteristics of biomolecules, such as instability and the constraint of granting the functionality to the biochip. Introduction of functional nanomaterials, recently being researched and developed, to biomolecules have been widely researched to develop the nanobiohybrid materials because such materials have the potential to enhance and extend the function of biomolecules on a biochip. The potential for applying nanobiohybrid materials is especially high in the field of bioelectronics. Research in bioelectronics is aimed at realizing electronic functions using the inherent properties of biomolecules. To achieve this, various biomolecules possessing unique properties have been combined with novel nanomaterials to develop bioelectronic devices such as highly sensitive electrochemical‐based bioelectronic sensing platforms, logic gates, and biocomputing systems. In this review, recently reported bioelectronic devices based on nanobiohybrid materials are discussed. The authors believe that this review will suggest innovative and creative directions to develop the next generation of multifunctional bioelectronic devices. 相似文献
100.
Lee Seokjin Trịnh Cao Sơn Lee Won Je Jeong Chan Young Truong Hai An Chung Namhyun Kang Chon-Sik Lee Hojoung 《Journal of plant research》2020,133(2):231-244
Journal of Plant Research - Plant growth promoting rhizobacteria (PGPR) are a group of bacteria that promote plants growth in the rhizosphere. PGPRs are involved in various mechanisms that... 相似文献