全文获取类型
收费全文 | 24517篇 |
免费 | 2419篇 |
国内免费 | 16篇 |
出版年
2022年 | 193篇 |
2021年 | 477篇 |
2020年 | 247篇 |
2019年 | 336篇 |
2018年 | 425篇 |
2017年 | 348篇 |
2016年 | 507篇 |
2015年 | 991篇 |
2014年 | 956篇 |
2013年 | 1315篇 |
2012年 | 1720篇 |
2011年 | 1675篇 |
2010年 | 1040篇 |
2009年 | 953篇 |
2008年 | 1355篇 |
2007年 | 1404篇 |
2006年 | 1317篇 |
2005年 | 1333篇 |
2004年 | 1296篇 |
2003年 | 1188篇 |
2002年 | 1197篇 |
2001年 | 257篇 |
2000年 | 178篇 |
1999年 | 289篇 |
1998年 | 317篇 |
1997年 | 220篇 |
1996年 | 210篇 |
1995年 | 187篇 |
1994年 | 191篇 |
1993年 | 197篇 |
1992年 | 181篇 |
1991年 | 167篇 |
1990年 | 150篇 |
1989年 | 177篇 |
1988年 | 172篇 |
1987年 | 148篇 |
1986年 | 151篇 |
1985年 | 170篇 |
1984年 | 184篇 |
1983年 | 169篇 |
1982年 | 236篇 |
1981年 | 230篇 |
1980年 | 178篇 |
1979年 | 146篇 |
1978年 | 161篇 |
1977年 | 130篇 |
1976年 | 135篇 |
1975年 | 117篇 |
1974年 | 121篇 |
1973年 | 112篇 |
排序方式: 共有10000条查询结果,搜索用时 281 毫秒
291.
Eugene C. Weinstein 《The Western journal of medicine》1965,102(6):416-418
Acute appendicitis still is a cause of considerable morbidity and now and then of death. The diagnostic accuracy in 316 patients operated on for acute appendicitis at Holy Cross Hospital was 76 per cent. In 24 of 239 cases of proved acute appendicitis, perforation had occurred, and the morbidity in those cases was three times that in the cases without perforation. Review of the cases did not reveal any clear-cut diagnostic criteria that might be used to predict perforation.A study of 30 patients with mesenteric lymphadenitis who were inadvertently operated on in the belief they had appendicitis, revealed that this condition is most likely to occur in young females with only a slight increase in the number of leukocytes. Although positive diagnosis of acute appendicitis is a difficult problem, the morbidity associated with needless operation is so much less than that which occurs in acute perforated appendicitis, that prompt exploration in any questionable case seems warranted. 相似文献
292.
A reagent composed of 0.2% p-phenylenediamine in 2 N NH4OH was used for the cytochemical demonstration of arbutin in plant tissue. Sections of fresh tissue were cut at 25-50 μ, mounted in a drop of the reagent, and allowed to stand uncovered 15-20 min before applying a coverslip. Arbutin stained dark blue to dark purple and was easily distinguished from other constituents of the cell, such as chlorogenic acid, isochlorogenic acid, caffeic acid, and quinic acid, which stained yellow, yellow-green, red or brown in color. The limit of sensitivity of the p-phenylenediamine-arbutin reaction was 1:100,000, as determined by spot-plate tests. 相似文献
293.
294.
295.
Summary Alpha-smooth muscle actin is currently considered a marker of smooth muscle cell differentiation. However, during various
physiologic and pathologic conditions, it can be expressed, sometimes only transiently, in a variety of other cell types,
such as cardiac and skeletal muscle cells, as well as in nonmuscle cells. In this report, the expression of actin mRNAs in
cultured rat capillary endothelial cells (RFCs) and aortic smooth muscle cells (SMCs) has been studied by Northern hybridization
in two-dimensional cultures seeded on individual extracellular matrix proteins and in three-dimensional type I collagen gels.
In two-dimensional cultures, in addition to cytoplasmic actin mRNAs which are normally found in endothelial cell populations,
RFCs expressed α-smooth muscle (SM) actin mRNA at low levels. α-SM actin mRNA expression is dramatically enhanced by TGF-β1. In addition, double immunofluorescence staining with anti-vWF and anti-α-SM-1 (a monoclonal antibody to α-SM actin) shows
that RFCs co-express the two proteins. In three dimensional cultures, RFCs still expressed vWF, but lost staining for α-SM
actin, whereas α-SM actin mRNA became barely detectable. In contrast to two-dimensional cultures, the addition of TGF-β1 to the culture media did not enhance α-SM actin mRNA in three-dimensional cultures, whereas it induced rapid capillary tube
formation. Actin mRNA expression was modulated in SMCs by extracellular matrix components and TGF-β1 with a pattern very different from that of RFCs. Namely, the comparison of RFCs with other cell types such as bovine aortic
endothelial cells shows that co-expression of endothelial and smooth muscle cell markers is very unique to RFCs and occurs
only in particular culture conditions. This could be related to the capacity of these microvascular endothelial cells to modulate
their phenotype in physiologic and pathologic conditions, particularly during angiogenesis, and could reflect different embryologic
origins for endothelial cell populations.
Supported by a Post-Doctoral Fellowship from the Swiss National Science Foundation (OK) and grant HL-RO1-28373 (JAM) from
the Department of Human Services, Public Health Service, Washington, D.C. 相似文献
296.
Jeffery R. Cook Barbara E. Crute Laura M. Patrone Joseph Gabriels Maureen E. Lane Robert G. van Buskirk 《In vitro cellular & developmental biology. Plant》1989,25(10):914-922
Summary We have analyzed the ability of the physical substratum to modulate both the ultrastructural and protein synthetic characteristics
of the Madin-Darby canine kidney (MDCK) renal cell line. When MDCK cells were seeded on Millipore Millicell CM microporous
membrane cell culture inserts they demonstrated a more columnar organization with an increase in cell density sixfold greater
than the same cells seeded on conventional plastic substrata. After 1 wk postseeding on the microporous membrane a partial
basal lamina was noted, with a contiguous basement membrane being apparent after 2 wk. One-dimensional sodium dodecyl sulfate
gel electrophoresis was used to analyze detergent-solubilized proteins from MDCK cells maintained on plastic substrata vs.
microporous membranes. When proteins were pulse-labeled with [35S]methionine, a 55 kDa protein was evident in the cytosolic extract of cells grown on collagen, laminin, and nontreated plastic
substrata; but this labeled protein was not evident in similar extracts from cells grown on collagen and laminin-coated microporous
membranes. To test if the polarized, basement-membrane secreting phenotype of the MDCK cells could be generated on a microporous
membrane without pretreatment with any extracellular matrix (ECM) components, cells were seeded on the Millipore Millicell
HA (cellulosic) microporous membrane. This type of substrata does not need a coating of ECM components for cell attachment.
A partial basement membrane was formed below cells where the basal surface of the cell was planar, but not in areas where
the cell formed large cytoplasmic extensions into the filter. This led us to the conclusion that the microporous nature of
the substrata can dictate both ultrastructural and protein synthetic activities of MDCK cells. Furthermore, we suggest that
both the planar nature of the basal surface and the microporosity of the substrate are corequisites for the deposition of
the basement membrane. 相似文献
297.
Joseph B. Mazzola Alan W. Neebe Christopher V. R. Dunn 《Flexible Services and Manufacturing Journal》1989,1(2):115-142
Early flexible manufacturing system (FMS) production planning models exhibited a variety of planning objectives; typically, these objectives were independent of the overall production environment. More recently, some researchers have proposed hierarchical production planning and scheduling models for FMS. In this article, we examine production planning of FMS in a material requirements planning (MRP) environment. We propose a hierarchical structure that integrates FMS production planning into a closed-loop MRP system. This structure gives rise to the FMS/MRP rough-cut capacity planning (FMRCP) problem, the FMS/MRP grouping and loading (FMGL) problem, and the FMS/MRP detailed scheduling problem. We examine the FMRCP and FMGL problems in detail and present mathematical programming models for each of these problems. In particular, the FMRCP problem is modeled as a generalized assignment problem (GAP), and a GAP-based heuristic procedure is defined for the problem. We define a two-phase heuristic for the FMGL problem and present computational experience with both heuristics. The FMRCP heuristic is shown to solve problems that exhibit a dependent-demand relation within the FMS and with FMS capacity utilization as high as 99 percent. The FMGL heuristic requires very little CPU time and obtains solutions to the test problems that are on average within 1.5 percent of a theoretical lower bound. This FMS/MRP production planning framework, together with the resulting models, constitutes an important step in the integration of FMS technology with MRP production planning. The hierarchical planning mechanism directly provides for system-level MRP planning priorities to induce appropriate production planning and control objectives on the FMS while simultaneously allowing for necessary feedback from the FMS. Moreover, by demonstrating the tractability of the FMRCP and FMGL problems, this research establishes the necessary groundwork upon which to explore systemwide issues pertaining to the coordination of the hierarchical structure. 相似文献
298.
Eugene L. Passer Rudean H. Leinaeng Lennel W. Birmingham Hector Cruz Chantale Dupervil Elizabeth J. Persaud Emil P. Dolensek 《Zoo biology》1989,8(4):357-365
This research determined the relationships between blood lead level and zinc protoporphyrin (ZnPROTO), protoporphyrin IX (PROTO), and free erythrocyte protoporphyrin (FEP) levels in a group of 18 ring teal ducks. Blood samples were drawn from two groups of ring teal ducks as part of the routine health maintenance program of the New York Zoological Park. One group of six teals had been exposed to what is believed to be lead-contaminated dust, while the second group of twelve teals was unexposed. Blood samples were analyzed for lead by flameless atomic absorption and for protoporphyrins by fluorescence. Blood lead level and log blood lead level had positive correlations with each of the protoporphyrins: the logarithmic correlations were better than the nonlogarithmic correlations, and PROTO correlated better than ZnPROTO. With one exception, PROTO levels were higher than ZnPROTO levels. The results suggest that PROTO, FEP, or ZnPROTO could serve as a biological indicator of lead poisoning in ring teal ducks. 相似文献
299.
Micronekton and macrozooplankton in the open waters near Antarctic ice edge zones (AMERIEZ 1983 and 1986) 总被引:4,自引:4,他引:0
Summary Micronekton and macrozooplankton assemblages (0–1000 m) were sampled from the open ocean in the vicinity of marginal ice zones in the southern Scotia and western Weddell Seas using midwater trawls. Small regional differences in species composition were found in the differing hydrographic settings with the Scotia Sea being slightly more diverse. Most species exhibited broad vertical ranges with no distinct pattern of vertical movement. Exceptions were mesopelagic fish and Salpa thompsoni which undertook diel vertical migrations. Biomass was high (2.4–3.1 g DW/m2), comparable to Pacific subarctic waters. Euphausia superba and Salpa tompsoni were the numerical and biomass dominants, representing over 50% of the total numbers and standing stocks. In terms of biomass, euphausiids were the most important group at shallow depths (0–200 m) but were surpassed by salps in the Scotia Sea and mesopelagic fish in the Weddell Sea when all depths down to 1000 m were considered. Pelagic fish biomass (3.3–4.4 g WW/m2) greatly exceeded published estimates for birds (0.025–0.070 g WW/m2), seals (0.068–0.089 g WW/m2) and whales (0.167 to 0.399 g WW/m2), making mesopelagic fish the most prevalent krill predators in the Antarctic oceanic system. 相似文献
300.
Sadis Matalon Joseph S. Beckman Michael E. Duffey Bruce A. Freeman 《Free radical biology & medicine》1989,6(6):557-564
The purpose of this study was to quantify the effects of extracellularly generated partially reduced oxygen species on active sodium (NA+) transport across the ventral toad skin, a well-studied epithelium. Sections of skin from decapitated toads were mounted in an Ussing chamber, bathed on both sides with electrolyte solution containing 500 μM xanthine and bubbled continuously with room air. The tissues were short-circuited, and short circuit current (Isc) and tissue resistance (Rt were monitored continuously with an automatic voltage clamp apparatus. Fifteen mU/ml of xanthine oxidase (XO), either purchased from Calbiochem or purified from cream, were instilled in either the apical (mucosal) or basolateral (serosal) baths at t = 0 and T = 10 min. Hydrogen peroxide (H2O2) concentrations increased to 200 μM within the first 20 min and then decreased, reaching a value of 40 μM by 60 min. Mean [H2O2] was 90 μM. Instillation of XO in the apical bath resulted in a large decrease in Isc and an increase in Rt, their values being 43% and 160% of their corresponding controls 85 min after the first instillation. Addition of superoxide dismutase and catalase completely prevented these changes. Instillation of XO in the basolateral bath had no effect. Similar physiological responses were obtained using the Calbiochem XO or the purified XO, which contained no measurable protease activity. It was concluded that extracellularly generated partially reduced oxygen species may interfere with active Na+ transport by possibly damaging apical Na+ channel proteins. 相似文献