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71.
Sergio Valente Ilaria Lepore Carmela Dell'Aversana Maria Tardugno Sabrina Castellano Gianluca Sbardella Stefano Tomassi Salvatore Di Maro Ettore Novellino Roberto Di Santo Roberta Costi Lucia Altucci Antonello Mai 《Biochimie》2012
Chemical manipulations undertaken on some bis(bromo- and dibromo-phenol) compounds previously reported by us as wide-spectrum epigenetic inhibitors let us to identify bis (bromo- and dibromo-methoxyphenyl) derivatives highly selective for PR-SET7 and EZH2 (compounds 4, 5, 9, and 10). Western blot analyses were carried out in U937 cells to determine the effects of such compounds on the methyl marks related to the tested enzymes (H3K4me1, H3K9me2, H4H20me1, and H3K27me3). The 1,5-bis(3-bromo-4-methoxyphenyl)penta-1,4-dien-3-one 4 (EC50 vs EZH2 = 74.9 μM), tested in U937 cells at 50 μM, induced massive cell death and 28% of granulocytic differentiation, highlighting the potential use of EZH2 inhibitors in cancer. 相似文献
72.
Carolina Carrizo García Massimo Guarnieri Ettore Pacini 《Acta Physiologiae Plantarum》2012,34(6):2341-2347
Ripe pollen has different soluble and insoluble carbohydrates in variable amounts. Pollen germination and pollen tube growth were studied in a tomato cultivar (Solanum lycopersicum L. cv. Platense) with atypical pollen among tomatoes due to its very low amount or absence of sucrose. In vitro assays were performed using a culture medium without carbohydrates to explore whether there is an autotrophic phase of pollen tube growth, and if there is, describe it, and to analyze the fluctuations of endogenous carbohydrates (soluble carbohydrates, starch, pectins, and callose). Pollen germination was fast (ca. 10 min) and a definite autotrophic phase was observed. Soluble carbohydrates and pectins showed the most substantial changes during this period, even after 10 min. A small amount of callose was observed in the ripe pollen and pollen tubes. Pectins were the most abundant pollen tube wall component. Pollen can be considered starchless; starch was not involved in the autotrophic phase of growth. Other types of substances must be connected with the carbohydrate metabolism, because the fluctuations of the different substances did not follow balanced stoichiometric relationships. Pollen germination and pollen tube elongation was sustained autotrophically, even though sucrose was absent and starch was negligible in pollen grains. The type of pollen reserves and the fast pollen tube formation could be selective advantages in this cultivar. 相似文献
73.
Post-pollination prefertilization drops affect germination rates of heterospecific pollen in larch and Douglas-fir 总被引:1,自引:0,他引:1
P von Aderkas M Nepi M Rise F Buffi M Guarnieri A Coulter K Gill P Lan S Rzemieniak E Pacini 《Sexual plant reproduction》2012,25(3):215-225
Pollen of larch (Larix?×?marschlinsii) and Douglas-fir (Pseudotsuga menziesii) was used in homospecific and heterospecific crosses. Germination of heterospecific pollen in ovulo was reduced in post-pollination prefertilization drops. This provides evidence of selection against foreign pollen by open-pollinated exposed ovules in these two sister taxa, which share the same type of pollination mechanism. Of the other prezygotic stages in pollen-ovule interactions, uptake of pollen by stigmatic hairs did not show any selection. Pollen tube penetration of the nucellus was similar for hetero- and homospecific pollen tubes, but heterospecific tubes only delivered gametes in one cross. To test for differences in the post-pollination prefertilization drops of each species, drops were gathered and analysed. Glucose and fructose were present in similar amounts in Douglas-fir and larch, while sucrose was found in larch only. Other carbohydrates such as xylose and melezitose were species-specific. In P. menziesii, sucrose is absent due to its conversion to glucose and fructose by apoplastic invertases. In contrast, Larix?×?marschlinsii drops have sucrose because they lack apoplastic invertases. The presence of invertase activity shows that the composition of gymnosperm post-pollination prefertilization drops is not static but dynamic. Drops of these two species also differed in their calcium concentrations. 相似文献
74.
Romolo Caniglia Elena Fabbri Sarah Cubaynes Olivier Gimenez Jean-Dominique Lebreton Ettore Randi 《Conservation Genetics》2012,13(1):53-64
Non-invasive genetic sampling (NGS) is increasingly used to estimate the abundance of rare or elusive species such as the
wolf (Canis lupus), which cannot be directly counted in forested mountain habitats. Wolf individual and familial home ranges are wide, potentially
connected by long-range dispersers, and their populations are intrinsically open. Appropriate demographic estimators are needed,
because the assumptions of homogeneous detection probability and demographic closeness are violated. We compiled the capture–recapture
record of 418 individual wolf genotypes identified from ca. 4,900 non-invasive samples, collected in the northern Italian
Apennines from January 2002 to June 2009. We analysed this dataset using novel capture–recapture multievent models for open
populations that explicitly account for individual detection heterogeneity (IDH). Overall, the detection probability of the
weakly detectable individuals, probably pups, juveniles and migrants (P = 0.08), was ca. six times lower than that of the highly detectable wolves (P = 0.44), probably adults and dominants. The apparent annual survival rate of weakly detectable individuals was lower (Φ = 0.66)
than those of highly detectable wolves (Φ = 0.75). The population mean annual finite rate of increase was λ = 1.05 ± 0.11,
and the mean annual size ranged from N = 117 wolves in 2003 to N = 233 wolves in 2007. This procedure, combining large-scale NGS and multievent IDH demographic models, provides the first
estimates of abundance, multi-annual trend and survival rates for an open large wolf population in the Apennines. These results
contribute to deepen our understanding of wolf population ecology and dynamics, and provide new information to implement sound
long-term conservation plans. 相似文献
75.
76.
77.
Vanna Fierabracci Pellegrino Masiello Michela Novelli Ettore Bergamini 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》1991,570(2):285-291
An amino acid analysis by reversed-phase high-performance liquid chromatography after precolumn derivatization with phenyl isothiocyanate was adapted to the determination of free amino acids in plasma or other biological fluids and in tissue homogenates. Preparation of samples included deproteinization by 3% sulphosalicylic acid, and careful removal under high vacuum of residual phenyl isothiocyanate after derivatization. A Waters Pico-Tag column (15 cm long) was used, immersed in a water-bath at 38°C. In rat or human plasma, separation of 23 individual amino acids, plus the unresolved pair tryptophan and ornithine, was obtained within 13 min. Including the time for column washing and re-equilibration, samples could be chromatographed at 23-min intervals. Variability was tested for each amino acid by calculating the coefficients of variation of retention times (less than 1% in the average) and peak areas (less than 4% for both intra-day and inter-day determinations). The linearity for each standard amino acid was remarkable over the concentration range 3–50 nmol/ml. The mean recovery of amino acid standards added to plasma prior to derivatization was 97 ± 0.8%, except for aspartate (82%) and glutamate (81%). This method is rapid (almost three samples per hour can be analysed, more than in any other reported technique), with satisfactory precision, sensitivity and reproducibility. Therefore, it is well suited for routine analysis of free amino acids in both clinical and research work. 相似文献
78.
Benedetti Ettore Iacovino Rosa Pedone Carlo Rossi Filomena Saviano Michele Isernia Carla Montesarchio Daniela de Napoli Lorenzo Piccialli Gennaro 《International journal of peptide research and therapeutics》1997,4(3):129-134
Summary An N- and C-protected derivative ofhomo-β-leucine, Fmoc-homo-β-(S)-leucine methyl ester, synthesized from the corresponding proteinogenic parent α-amino acid in enantiopure form has been
fully characterized in the solid state by X-ray diffraction analysis. The crystal conformation of this new residue indicates
and extended conformation for thishomo-β-residue, with the ϕ torsion angle being more constrained than the μ and ψ angles. 相似文献
79.
Murray Goodman Ralph-Heiko Mattern Peter Gantzel Antonello Santini Rosa Iacovino Michele Saviano Ettore Benedetti 《Journal of peptide science》1998,4(4):229-238
The molecular basis of sweet taste was investigated by carrying out the crystal state conformational analysis by X-ray diffraction of the following dipeptide taste igands:N-3,3-dimethylbutyl-aspartyl-phenylalanine methyl ester, I (N-DMB-Asp-Phe-OMe), its sodium salt (N-DMB-Asp-Phe-ONa), II , aspartyl-D -2-aminobutyric acid-(S)-α-ethylbenzylamide, III (Asp-D -Abu-(S)-α-ethylbenzylamide), aspartyl-N′-((2,2,5,5-tetramethylcyclopentanyl)-carbonyl)-(R)-1,1-diamino-ethane, IV (Asp-(R)-gAla-TMCP), and aspartyl-D -valine-(R)-α-methoxymethylbenzyl amide, V (Asp-D -Val-(R)-α-methoxymethylbenzylamide). With the exception of the sodium salt II , all compounds are sweet-tasting, showing in some cases considerable potency enhancement with respect to sucrose. The results of this study confirm the earlier model that an ‘L-shape’ molecular array is essential for eliciting sweet taste for dipeptide-like ligands. In addition, it was established that (i) substitution of the N-terminal group does not inhibit sweet taste, if its zwitterionic character is maintained; (ii) a hydrophobic group located between the stem and the base of the L-shape could be responsible for sweetness potency enhancement, as found in I, III and IV ; in fact, the extraordinary potency of the N-alkylated analogue I would support a model with an additional hydrophobic binding domain above the base of the ‘L’; (iii) removal of the methyl ester at the C-terminus of compound I with the salt formation gives rise to the tasteless compound II ; (iv) for the first time all possible side-chain conformers (g−,g+andt) for the N-substituted aspartyl residue were observed; and (v) a retro-inverso modification, incorporated at position 2 of the dipeptide chain, confers greater flexibility to the molecule, as demonstrated by the contemporary presence of six conformationally distinct independent molecules in the unit cell and yet sweet taste properties are maintained, as found in IV . © 1998 European Peptide Society and John Wiley & Sons, Ltd. 相似文献
80.
Ettore Benedetti Michele Saviano Rosa Iacovino Carlo Pedone Antonello Santini Marco Crisma Fernando Formaggio Claudio Toniolo Quirinus B. Broxterman Johan Kamphuis 《Biopolymers》1998,46(7):433-443
The crystal-state preferred conformations of six Nα-blocked pentapeptide esters, each containing four helicogenic, achiral α-aminoisobutyric acid (Aib) residues followed by one chiral L -valine (L -Val) or Cα-methyl-L -valine [(αMe)Val] residue at the C-terminus, have been assessed by x-ray diffraction analysis. In all of the compounds the (Aib)4 sequence is folded in a regular 310-helical conformation. In the four pentapeptides characterized by the L -(αMe)Val residue two conformationally distinct molecules occur in the asymmetric unit. Conversely, only one molecule is observed in the asymmetric unit of two pentapeptides with the C-terminal L -Val residue. In the L -Val based peptides the helical screw sense of the (Aib)4 sequence is right-handed, whereas in the L (αMe)Val analogues both right- and left-handed helical screw senses concomitantly occur in the two crystallographically independent molecules. © 1998 John Wiley & Sons, Inc. Biopoly 46: 433–443, 1998 相似文献