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471.
A new bile alcohol, 5 beta-cholestanehexol, was identified in the urine of healthy humans as the glucuronide. The bile alcohol glucuronide fraction was isolated by an ion exchange chromatography on piperidinohydroxypropyl Sephadex LH-20. After enzymatic hydrolysis, the bile alcohols were converted into trimethylsilyl ether derivatives and analyzed by a combination of gas-liquid chromatography and mass spectrometry. The major bile alcohol was 27-nor-5 beta-cholestane-3 alpha,7 alpha,12 alpha,24,25-pentol. As minor constituents the following C26 and C27 bile alcohols were identified: 27-nor-5 beta-cholestane-3 alpha,7 alpha,12 alpha,24,25,26-hexol, 5 beta-cholestane-3 alpha,7 alpha,12 alpha,24,25-pentol, 5 beta-cholestane-3 alpha,7 alpha,12 alpha,24,26-pentol, 5 beta-cholestane-3 alpha,7 alpha,12 alpha,25,26-pentol. In addition to these bile alcohols, a new bile alcohol was identified as a sixth component of the urinary bile alcohols. The structure was assigned as (24S)-5 beta-cholestane-3 alpha,7 alpha,12 alpha,24,25,26-hexol by the direct comparison of mass spectral data and chromatographic properties with synthetic standard. The average daily excretion of the new bile alcohol was 28.6 micrograms and 3.0% of the total bile alcohols. The presence of 27-nor-5 beta-cholestane-3 alpha,7 alpha,12 alpha,24,25-pentol and 27-nor-5 beta-cholestane-3 alpha,7 alpha,12 alpha,24,25,26-hexol suggests that 26-hydroxylation of 5 beta-cholestane-3 alpha,7 alpha,12 alpha,24,25-pentol is most likely for the biosynthesis of this new bile alcohol. 相似文献
472.
473.
M Kuramoto T Kuwashima K Morishita T Fukuda M Kawauchi T Kita N Komi 《Nihon seirigaku zasshi. Journal of the Physiological Society of Japan》1979,41(1):1-5
To determine whether the electric activities of the stomach and duodenum are related, the gastroduodenal junction area of the stomach of dogs was paced through implanted bipolar silver electrodes. It was found that the pacing rate of the basic electrical rhythm (BER) in the antrum of the stomach could be increased to a maximum of 8.9 cycles/min, without affecting the BER frequency in the duodenum. Conversely when the pacing rate of the BER in the duodenum was increased to a maximum of 24 cycles/min, the BER frequency in the antrum remained unaffected. These results show that the BER in the stomach and duodenum are independent, and imply that the BER cycles in the stomach and duodenum are due to the presence of two independent pace-maker areas. 相似文献
474.
Neural noise has been successfully used as a real time guide for verification of tumor site during stereotactic biopsy of brain tumors. This technique is a useful adjunct to other methods for tumor site verification and may possibly give pathophysiological information in peritumoral areas. 相似文献
475.
Hiroki Takakuwa Tetsu Yamashiro Mai Q. Le Lien S. Phuong Hiroichi Ozaki Ryota Tsunekuni Tatsufumi Usui Hiroshi Ito Tsuyoshi Yamaguchi Toshihiro Ito Toshiyuki Murase Etsuro Ono Koichi Otsuki 《Microbiology and immunology》2010,54(1):58-62
To estimate the prevalence of influenza A subtype H5N1 viruses among domestic ducks in the period between October and November 2006 when H5N1 outbreaks had been absent, 1106 healthy ducks raised in northern Vietnam were collected. Inoculation of all throat and cloacae samples into embryonated eggs resulted in the isolation of subtype H3N8 in 13 ducks, but not H5N1 viruses. Serological analyses demonstrated that five ducks (0.45%) solely developed H5N1 subtype-specific hemagglutinin-inhibiting and neuraminidase-inhibiting antibodies together with anti-non-structural protein 1 antibodies. The results suggested that the ducks were naturally infected with H5N1 viruses when obvious H5N1 outbreaks were absent. 相似文献