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821.
The identification of interacting species and elucidation of their mode of interaction may be crucial to understand ecosystem-level processes. We analysed the activity and identity of bacterial epibionts in cultures of Daphnia galeata and of natural daphnid populations. Epibiotic bacteria incorporated considerable amounts of dissolved organic carbon (DOC), as estimated via uptake of tritiated leucine: three times more tracer was consumed by microbes on a single Daphnia than in 1 ml of lake water. However, there was virtually no incorporation if daphnids were anaesthetised, suggesting that their filtration activity was essential for this process. Microbial DOC uptake could predominantly be assigned to microbes that were located on the filter combs of daphnids, where the passage of water would ensure a continuously high DOC supply. Most of these bacteria were Betaproteobacteria from the genus Limnohabitans. Specifically, we identified a monophyletic cluster harbouring Limnohabitans planktonicus that encompassed sequence types from D. galeata cultures, from the gut of Daphnia magna and from daphnids of Lake Zurich. Our results suggest that the epibiotic growth of bacteria related to Limnohabitans on Daphnia spp. may be a widespread and rather common phenomenon. Moreover, most of the observed DOC flux to Daphnia in fact does not seem to be associated with the crustacean biomass itself but with its epibiotic microflora. The unexplored physical association of daphnids with heterotrophic bacteria may have considerable implications for our understanding of carbon transfer in freshwater food webs, that is, a trophic ‘shortcut'' between microbial DOC uptake and predation by fish. 相似文献
822.
Susanna C. Manrubia Esteban Domingo Ester Lázaro 《Philosophical transactions of the Royal Society of London. Series B, Biological sciences》2010,365(1548):1943-1952
Since the introduction of the quasispecies and the error catastrophe concepts for molecular evolution by Eigen and their subsequent application to viral populations, increased mutagenesis has become a common strategy to cause the extinction of viral infectivity. Nevertheless, the high complexity of virus populations has shown that viral extinction can occur through several other pathways apart from crossing an error threshold. Increases in the mutation rate enhance the appearance of defective forms and promote the selection of mechanisms that are able to counteract the accelerated appearance of mutations. Current models of viral evolution take into account more realistic scenarios that consider compensatory and lethal mutations, a highly redundant genotype-to-phenotype map, rough fitness landscapes relating phenotype and fitness, and where phenotype is described as a set of interdependent traits. Further, viral populations cannot be understood without specifying the characteristics of the environment where they evolve and adapt. Altogether, it turns out that the pathways through which viral quasispecies go extinct are multiple and diverse. 相似文献
823.
Idit Sagiv-Barfi Ester Weiss Alexander Levitzki 《Bioorganic & medicinal chemistry》2010,18(17):6404-6413
We report here on a class of quinazoline molecules that inhibit T cell proliferation. The most potent compound N-p-tolyl-2-(3,4,5-trimethoxyphenyl)quinazolin-4-amine (S101) and its close analogs were found to inhibit the proliferation of T cells from human peripheral blood mononuclear cells (PBMC) and Jurkat cells, with IC50 in the sub-micromolar range. The inhibitor induced G2 cell cycle arrest but did not inhibit IL-2 secretion. The anti-proliferative effect correlated with inhibition of the tyrosine phosphorylation of SLP-76, a molecular element in the signaling pathway of the T cell receptor (TCR). The inhibitor restrained proliferation of lymphocytes with much higher potency than non-hematopoietic cells. This new class of specific T cell proliferation inhibitors may serve as lead molecules for the development of agents aimed at diseases in which T cell signaling plays a role and agents to induce tolerance to grafted tissues or organs. 相似文献
824.
825.
Background
The secondary structure of folded RNA sequences is a good model to map phenotype onto genotype, as represented by the RNA sequence. Computational studies of the evolution of ensembles of RNA molecules towards target secondary structures yield valuable clues to the mechanisms behind adaptation of complex populations. The relationship between the space of sequences and structures, the organization of RNA ensembles at mutation-selection equilibrium, the time of adaptation as a function of the population parameters, the presence of collective effects in quasispecies, or the optimal mutation rates to promote adaptation all are issues that can be explored within this framework. 相似文献826.
827.
Isabel M Pires Zuzana Bencokova Chris McGurk Ester M Hammond 《Cell cycle (Georgetown, Tex.)》2010,9(13):2502-2507
Severe hypoxia has been demonstrated to induce a replication arrest which is associated with decreased levels of nucleotides. Chk1 is rapidly phosphorylated in response to severe hypoxia and in turn deactivates TLK1 through phosphorylation. Loss of Chk1 has been shown to sensitize cells to hypoxia/reoxygenation. After short (acute) exposure to hypoxia this is due to an increased rate of reoxygenation-induced replication restart and subsequent p53-dependent apoptosis. After longer (chronic) exposure to hypoxia S phase cells do not undergo reoxygenation-induced replication restart. Cells exposed to these levels of hypoxia are however sensitive to loss of Chk1. This suggests a new role for Chk1 in the cell cycle response to reoxygenation.Key words: hypoxia, reoxygenation, replication restart, Chk1, TLK1 相似文献
828.
Convergent adaptation to a marginal habitat by homoploid hybrids and polyploid ecads in the seaweed genus Fucus 总被引:1,自引:0,他引:1
Hybridization and polyploidy are two major sources of genetic variability that can lead to adaptation in new habitats. Most species of the brown algal genus Fucus are found along wave-swept rocky shores of the Northern Hemisphere, but some species have adapted to brackish and salt marsh habitats. Using five microsatellite loci and mtDNA RFLP, we characterize two populations of morphologically similar, muscoides-like Fucus inhabiting salt marshes in Iceland and Ireland. The Icelandic genotypes were consistent with Fucus vesiculosus x Fucus spiralis F1 hybrids with asymmetrical hybridization, whereas the Irish ones consisted primarily of polyploid F. vesiculosus. 相似文献
829.
Revisiting synchronous gamete release by fucoid algae in the intertidal zone: fertilization success and beyond? 总被引:1,自引:0,他引:1
In the marine environment, both external fertilization and settlementare critical processes linking adult and early juvenile life-historyphases. The success of both processes can be tightly linkedin organisms lacking a larval dispersive phase. This reviewfocuses on synchronous gamete release (= spawning) in fucoidalgae. These brown macroalgae are important components of temperateintertidal ecosystems in many parts of the world, and achievesynchronous gamete release by integrating various environmentalsignals. Photosynthesis-dependent sensing of boundary-layerinorganic carbon fluxes, as well as blue light and green lightsignals, possibly perceived via a chloroplast-located photoreceptor(s),are integrated into pathways that restrict gamete release toperiods of low water motion. Avoidance of turbulent and/or highflow conditions in the intertidal zone allows high levels offertilization success in this group. Temporal patterns and synchronyof spawning in natural populations are reviewed. Most species/populationshave a more or less semilunar periodicity, although phase differencesoccur both between and within species at different geographicallocations, raising the possibility that tidal and diurnal cuesare more important than semilunar cues in entraining the response.The ecological and evolutionary role(s) of synchronous spawningin the intertidal zone are considered, particularly with regardto hybridization/reproductive isolation in species complexes,and reproductive versus recruitment assurance in the intertidalzone, where synchronous spawning during calm periods may beimportant for recruitment assurance in addition to fertilizationsuccess. Ways in which the roles of spawning synchrony couldbe tested in closely related species with contrasting matingsystems (outcrossing versus selfing) are discussed. 相似文献
830.
Santovito G Cassini A Piccinni E 《Comparative biochemistry and physiology. Toxicology & pharmacology : CBP》2006,143(4):444-454
In the present study, we describe the purification and molecular characterization of Cu,Zn superoxide dismutase (SOD) from Trematomus bernacchii, a teleost widely distributed in many areas of Antarctica, that plays a pivotal role in the Antarctic food chain. The amino acid and cDNA sequences have been obtained using both biochemical and molecular biology approaches and are compared with Cu,Zn SODs from other fishes. Assessment of the primary sequences highlights that the catalytically important residues are fully conserved in Cu,Zn SOD from T. bernacchii. Phylogenetic analyses performed on Cu,Zn SOD amino acid sequences permit speculation regarding the evolution of this protein. In particular, the data confirms the erratic differentiation of these proteins and concurs with the theory of the "unclock-like" behaviour of Cu,Zn SOD evolution. 相似文献