Phenotypic and genotypic characterization of Klebsiella pneumoniae isolates recovered from nonhuman primates

Klebsiella pneumoniae is a zoonotic, Gram-negative member of the family Enterobacteriaceae and is the causative agent of nosocomial septicemic, pneumonic, and urinary tract infections. Recently, pathogenic strains of K. pneumoniae sharing a hypermucoviscosity (HMV) phenotype have been attributed to multisystemic abscessation in both human and nonhuman primates. Although K. pneumoniae is a well-recognized zoonotic agent, there is a lack of general information including adequate diagnostic methods or treatments for nonhuman primates. In an effort to increase the body of knowledge of this enigmatic pathogen, K. pneumoniae isolates from African green monkeys (Chlorocebus aethiops sabaeus) on the island of St. Kitts, West Indies were genotypically and phenotypically characterized. Genetic fingerprints generated by PCR-mediated genomic fingerprinting, phenotypic characterization, and antimicrobial susceptibility all identified a high degree of similarity between the HMV and non-HMV K. pneumoniae isolates. The results obtained from this work will help establish a baseline for the development of efficacious diagnostic methods and treatment strategies for both human and nonhuman primates.     

The inflorescence in Commelinaceae

The structure of the synflorescence and the flowering unit (inflorescence) in Commelinaceae are characterized. The synflorescence is polytelic and the basic inflorescence type is a terminal pedunculate thyrse with an indeterminate central axis to which several to many cincinni are attached. Each thyrse is a florescence, and each cincinnus is a partial florescence. Variations mainly in the number of cincinni and in the number of flowers on each cincinnus determine the main differences found in the inflorescences of the different genera. Hypothesized developmental processes are described, with a view to finding relationships among different models characterized in the family as well as defining characters for cladistic studies, which may be useful to depict all the variations observed and serve as a guide for future phylogenetic studies.     

Advances in maize genomics: the emergence of positional cloning

Positional cloning has been and remains a powerful method for gene identification in Arabidopsis. With the completion of the rice genome sequence, positional cloning in rice also took off, including the cloning of several quantitative trait loci. Positional cloning in cereals such as maize whose genomes are much larger than that of rice was considered near impossible because of the vast amounts of repetitive DNA. However, conservation of synteny across the cereal genomes, in combination with new maize resources, has now made positional cloning in maize feasible. In fact, a chromosomal walk is usually much faster than the more traditional method of gene isolation in maize by transposon tagging.     

Beclin1-binding UVRAG targets the class C Vps complex to coordinate autophagosome maturation and endocytic trafficking

Autophagic and endocytic pathways are tightly regulated membrane rearrangement processes that are crucial for homeostasis, development and disease. Autophagic cargo is delivered from autophagosomes to lysosomes for degradation through a complex process that topologically resembles endosomal maturation. Here, we report that a Beclin1-binding autophagic tumour suppressor, UVRAG, interacts with the class C Vps complex, a key component of the endosomal fusion machinery. This interaction stimulates Rab7 GTPase activity and autophagosome fusion with late endosomes/lysosomes, thereby enhancing delivery and degradation of autophagic cargo. Furthermore, the UVRAG-class-C-Vps complex accelerates endosome-endosome fusion, resulting in rapid degradation of endocytic cargo. Remarkably, autophagosome/endosome maturation mediated by the UVRAG-class-C-Vps complex is genetically separable from UVRAG-Beclin1-mediated autophagosome formation. This result indicates that UVRAG functions as a multivalent trafficking effector that regulates not only two important steps of autophagy - autophagosome formation and maturation - but also endosomal fusion, which concomitantly promotes transport of autophagic and endocytic cargo to the degradative compartments.     

Suppressor cells present in the spleens of Trypanosoma cruzi-infected mice.

Infection with Trypanosoma cruzi decreases the ability of spleen cells from mice to respond to either T cell, concanavalin A (Con A), or B cell, lipopolysaccharide (LPS), mitogens. The effect of infection on the mitogenic response depends on the elapsed time between the day of infection and the time of mitogen presentation. Responses early in infection are normal, whereas later responses to either mitogen are depressed. Spleen cells from late trypanosome-infected mice inhibit the ability of normal spleen cells to respond to Con A or LPS. The cell in the T. cruzi-infected spleen cells responsible for this effect is nonadherent, sensitive to treatment with anti-mouse thymus serum plus complement, but insensitive to treatment with anti-immunoglobulin plus complement. These data indicate that infection with T. cruzi elicits over time the generation of T cells suppressive to T and B cell mitogenic responses.     

Economic Botany and ethnobotany in al-Andalus (Iberian Peninsula: Tenth-fifteenth centuries), an unknown heritage of Mankind

The Hispano-Arabic culture in the Iberian Middle Ages is a major chapter in the history of the use and knowledge of plants. The Andalusi agronomists, botanists and physicians assimilated their heritage of Iberian, Hispano-Roman, and Hispano-Visigothic cultures with North-African and Eastern influences. They developed a profound knowledge of the plant world and managed a high diversity of species. A part of this ethnobotanical and agronomic heritage was transmitted not only to the local cultures and generations that followed, but also to peoples on the other side of the Atlantic Ocean by the Spanish colonists in the New World. This paper presents a study of the principal works of the so-called Andalusi Agronomic School (10th-15th centuries) and their agronomist authors: Arib ben Said, Ibn Wafid, Ibn Hayyay, Abu l-Jayr, Ibn Bassal, al-Tignari, Ibn al-Awwam and Ibn Luyun. We also raise questions about Andalusi ethnobotany, the introduction of Oriental species in the Iberian Peninsula and the prospects for ethnobotanical research through the philological study of Hispano-Arabic writings.