全文获取类型
收费全文 | 192篇 |
免费 | 12篇 |
出版年
2023年 | 1篇 |
2022年 | 2篇 |
2021年 | 1篇 |
2020年 | 1篇 |
2018年 | 1篇 |
2016年 | 4篇 |
2015年 | 5篇 |
2014年 | 10篇 |
2013年 | 13篇 |
2012年 | 13篇 |
2011年 | 19篇 |
2010年 | 13篇 |
2009年 | 11篇 |
2008年 | 12篇 |
2007年 | 12篇 |
2006年 | 13篇 |
2005年 | 9篇 |
2004年 | 9篇 |
2003年 | 12篇 |
2002年 | 18篇 |
2001年 | 1篇 |
2000年 | 2篇 |
1999年 | 5篇 |
1998年 | 1篇 |
1997年 | 4篇 |
1996年 | 1篇 |
1995年 | 1篇 |
1994年 | 1篇 |
1993年 | 2篇 |
1992年 | 1篇 |
1991年 | 1篇 |
1990年 | 1篇 |
1989年 | 2篇 |
1988年 | 1篇 |
1986年 | 1篇 |
排序方式: 共有204条查询结果,搜索用时 15 毫秒
201.
Karel Jank Espen Jensen Georg Becher 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》1999,734(2):487
A method for the isolation of polychlorinated biphenyls (PCBs) from human blood using solid-phase extraction (SPE) has been developed. The procedure incorporates decomposition of lipids by concentrated sulphuric acid directly on the SPE column. Conditions for transferring PCBs onto the SPE column and washing the decomposed blood components from the SPE column were optimised. After clean-up the extracts were analysed using gas chromatography with electron capture detection. An average recovery of PCBs from spiked blood samples was about 78±8% and an average precision was about 109±7%. Quantitation has been done using four internal standards and calibration curves based on five concentration levels. Low procedural blanks made it possible to determine PCBs in blood quantitatively at a level down to 2–10 pg g−1. The integrated method for blood is fast, less laborious than methods using liquid–liquid extraction and has a low consumption of organic solvents. 相似文献
202.
203.
Christian Berger Inger Helene Madshus Espen Stang 《Experimental cell research》2012,318(20):2578-2591
The monoclonal antibody C225 (Cetuximab) blocks binding of ligand to the epidermal growth factor receptor (EGFR). In addition, it is known that incubation with C225 induces endocytosis of the EGFR. This endocytosis has previously been shown to be increased when C225 is combined with an additional monoclonal anti-EGFR antibody. However, the effects of antibody combinations on EGFR activation, endocytosis, trafficking and degradation have been unclear. By binding a secondary antibody to the C225-EGFR complex, we here demonstrate that a combination of antibodies can efficiently internalize and degrade the EGFR. Although the combination of antibodies activated the EGFR kinase and induced ubiquitination of the EGFR, the kinase activity was not required for internalization of the EGFR. In contrast to EGF-induced EGFR down-regulation, the antibody combination efficiently degraded the EGFR without initiating downstream proliferative signaling. The antibody-induced internalization of EGFR was found not to depend on clathrin and/or dynamin, but depended on actin polymerization, suggesting induction of macropinocytosis. Macropinocytosis may cause internalization of large membrane areas, and this could explain the highly efficient internalization of the EGFR induced by combination of antibodies. 相似文献
204.