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21.
We compared the richness and abundance of free-feeding herbivore insects (sap-sucking and leaf-chewing), leaf herbivory damage, leaf toughness and total phenolic content between two ontogenetic stages (juvenile and reproductive) of Handroanthus spongiosus (Rizzini) S. O. Grose (Bignoniaceae) throughout the rainy season in a Brazilian seasonally dry tropical forest. Twenty marked individuals of H. spongiosus were sampled per ontogenetic stage in each period of the rainy season (beginning, middle, and end). Herbivore richness and abundance did not differ between ontogenetic stages, but higher percentage of leaf damage, higher concentration of phenolic compounds, and lower leaf toughness were observed for juvenile individuals. The greatest morphospecies abundance was found at the beginning of the rainy season, but folivory increment was higher at the end, despite the fact that leaf toughness and total phenolic content increased in the same period. No significant relationships between leaf damage and both total phenolic content and leaf toughness were observed. These results suggest that insect richness and abundance do not track changes in foliage quality throughout plant ontogeny, but their decrease along rainy season confirms what was predicted for tropical dry forests. The general trends described in the current study corroborate those described in the literature about herbivores and plant ontogeny. However, the lack of relationship between herbivore damage and the two plant attributes considered here indicates that the analyses of multiple defensive traits (the defense syndrome) must be more enlightening to determine the mechanisms driving temporal and spatial patterns of herbivore attack. 相似文献
22.
Bougouma EC Tiono AB Ouédraogo A Soulama I Diarra A Yaro JB Ouédaogo E Sanon S Konaté AT Nébié I Watson N Sanza M Dube TJ Sirima SB 《Malaria journal》2012,11(1):154
ABSTRACT: BACKGROUND: Genetic factors play a key role in determining resistance/susceptibility to infectious disease. Susceptibility of the human host to malaria infection has been reported to be influenced by genetic factors, which could be confounders if not taken into account in the assessment of the efficacy of interventions against malaria. This study aimed to assess the relationship between haemoglobin genotypes and malaria in children under five years in a site being characterized for future malaria vaccine trials. METHODS: The study population consisted of 452 children living in four rural villages. Hb genotype was determined at enrolment. Clinical malaria incidence was evaluated over a one-year period using combined active and passive surveillance. Prevalence of infection was evaluated via bi-annual cross-sectional surveys. At each follow-up visit, children received a brief clinical examination and thick and thin blood films were prepared for malaria diagnosis. A clinical malaria was defined as Plasmodium falciparum parasitaemia >2,500 parasites/ul and axillary temperature [greater than or equal to]37.5degreesC or reported fever over the previous 24 hours. RESULTS: Frequencies of Hb genotypes were 73.2% AA; 15.0% AC; 8.2% AS; 2.2% CC; 1.1% CS and 0.2% SS. Prevalence of infection at enrolment ranged from 61.9%-54.1% among AA, AC and AS children. After one year follow-up, clinical malaria incidence (95% CI) (episodes per person-year) was 1.9 (1.7-2.0) in AA, 1.6 (1.4-2.1) in AC, and 1.7 (1.4-2.0) in AS children. AC genotype was associated with lower incidence of clinical malaria relative to AA genotype among children aged 1-2 years [rate ratio (95% CI) 0.66 (0.42-1.05)] and 2-3 years [rate ratio (95% CI) 0.37 (0.18-0.75)]; an association of opposite direction was however apparent among children aged 3-4 years. AS genotype was associated with lower incidence of clinical malaria relative to AA genotype among children aged 2-3 years [rate ratio (95% CI) 0.63 (0.40-1.01)]. CONCLUSIONS: In this cohort of children, AC or AS genotype was associated with lower risk of clinical malaria relative to AA genotype only among children aged one to three years. It would be advisable for clinical studies of malaria in endemic regions to consider haemoglobin gene differences as a potentially important confounder, particularly among younger children. 相似文献
23.
Tenbaum SP Ordóñez-Morán P Puig I Chicote I Arqués O Landolfi S Fernández Y Herance JR Gispert JD Mendizabal L Aguilar S Ramón y Cajal S Schwartz S Vivancos A Espín E Rojas S Baselga J Tabernero J Muñoz A Palmer HG 《Nature medicine》2012,18(6):892-901
The Wnt–β-catenin and PI3K-AKT-FOXO3a pathways have a central role in cancer. AKT phosporylates FOXO3a, relocating it from the cell nucleus to the cytoplasm, an effect that is reversed by PI3K and AKT inhibitors. Simultaneous hyperactivation of the Wnt–β-catenin pathway and inhibition of PI3K-AKT signaling promote nuclear accumulation of β-catenin and FOXO3a, respectively, promoting cell scattering and metastasis by regulating a defined set of target genes. Indeed, the anti-tumoral AKT inhibitor API-2 promotes nuclear FOXO3a accumulation and metastasis of cells with high nuclear β-catenin content. Nuclear β-catenin confers resistance to the FOXO3a-mediated apoptosis induced by PI3K and AKT inhibitors in patient-derived primary cultures and in corresponding xenograft tumors in mice. This resistance is reversed by XAV-939, an inhibitor of Wnt–β-catenin signaling. In the presence of high nuclear β-catenin content, activation of FOXO3a by PI3K or AKT inhibitors makes it behave as a metastasis inductor rather than a proapoptotic tumor suppressor. We show that it is possible to evaluate the β-catenin status of patients' carcinomas and the response of patient-derived cells to target-directed drugs that accumulate FOXO3a in the nucleus before deciding on a course of treatment. We propose that this evaluation could be essential to the provision of a safer and more effective personalized treatment. 相似文献
24.
25.
MA Espósito P Almirón I Gatti VP Cravero FS Anido EL Cointry 《Genetics and molecular research : GMR》2012,11(3):2729-2732
In breeding programs, a large number of F(2) individuals are required to perform the selection process properly, but often few such plants are available. In order to obtain more F(2) seeds, it is necessary to multiply the F(1) plants. We developed a rapid, efficient and reproducible protocol for in vitro shoot regeneration and rooting of seeds using 6-benzylaminopurine. To optimize shoot regeneration, basic medium contained Murashige and Skoog (MS) salts with or without B5 Gamborg vitamins and different concentrations of 6-benzylaminopurine (25, 50 and 75 μM) using five genotypes. We found that modified MS (B5 vitamins + 25 μM 6-benzylaminopurine) is suitable for in vitro shoot regeneration of pea. Thirty-eight hybrid combinations were transferred onto selected medium to produce shoots that were used for root induction on MS medium supplemented with α-naphthalene-acetic acid. Elongated shoots were developed from all hybrid genotypes. This procedure can be used in pea breeding programs and will allow working with a large number of plants even when the F(1) plants produce few seeds. 相似文献
26.
Eight helminth taxa were found parasitizing Chaunus marinus (n = 40) and Cranopsis valliceps (n = 40) from the Parque Estatal Lagunas Yalahau, Yucatan, Mexico. Seven taxa (2 digeneans: Langeronia macrocirra, Mesocoelium monas; 1 acanthocephalan: Oncicola sp.; 3 nematodes: Rhabdias füleborni, Aplectana itzocanensis, Cruzia morleyi; and a nematode larva) were found in C. marinus, while 4 taxa (all nematodes: Rhabdias fuelleborni, Aplectana itzocanensis, Ozwaldocruzia sp., and a nematode larva) were present in C. valliceps. Nematodes, particularly A. iztocanensis, showed high prevalence, mean abundance, and mean intensity values for both species of amphibians. The occurrence of R. fuelleborni, M. monas, L. macrocirra, and C. morleyi in these amphibians from the Yucatan Peninsula confirms their neotropical distribution, while the presence of A. itzocanensis increases its geographical distribution, suggesting a preference by neotropical, rather than neartic areas. 相似文献
27.
Costa NJ Bombardieri CR Kuribayashi JS de Camargo MM Andrade LH Kagohara E Espósito BP 《化学与生物多样性》2008,5(10):2156-2159
It is shown that metal complexes of the biodegradable ligand ethylenediaminedisuccinic acid (edds) present antimicrobial activity towards fungi and bacteria. [Cd(edds)], in particular, is more toxic than free Cd2+ to Aspergillus niger, behaving as a 'Trojan Horse' in the facilitated delivery of the toxic metal into the fungus. 相似文献
28.
Loïc Pellissier Nadir Alvarez Anahí Espíndola Julien Pottier Anne Dubuis Jean‐Nicolas Pradervand Antoine Guisan 《Ecography》2013,36(5):541-550
The observation of non‐random phylogenetic distribution of traits in communities provides evidence for niche‐based community assembly. Environment may influence the phylogenetic structure of communities because traits determining how species respond to prevailing conditions can be phylogenetically conserved. In this study, we investigate the variation of butterfly species richness and of phylogenetic α‐ and β‐diversities along temperature and plant species richness gradients. Our study indicates that butterfly richness is independently positively correlated to temperature and plant species richness in the study area. However, the variation of phylogenetic α‐ and β‐diversities is only correlated to temperature. The significant phylogenetic clustering at high elevation suggests that cold temperature filters butterfly lineages, leading to communities mostly composed of closely related species adapted to those climatic conditions. These results suggest that in colder and more severe conditions at high elevations deterministic processes and not purely stochastic events drive the assemblage of butterfly communities. 相似文献
29.
Maurício Luiz Vilela Daniela de Pita-Pereira Carina Graser Azevedo Rodrigo Espíndola Godoy Constan?a Britto Elizabeth Ferreira Rangel 《Memórias do Instituto Oswaldo Cruz》2013,108(5):578-585
Phlebotomine sandflies were captured in rural settlement and periurban
areas of the municipality of Guaraí in the state of Tocantins (TO), an endemic
area of American cutaneous leishmaniasis (ACL). Forty-three phlebotomine species
were identified, nine of which have already been recognised as ACL vectors.
Eleven species were recorded for the first time in TO. Nyssomyia
whitmani was the most abundant species, followed by
Evandromyia bourrouli, Nyssomyia antunesi
and Psychodopygus complexus. The Shannon-Wiener diversity index
and the evenness index were higher in the rural settlement area than in the
periurban area. The evaluation of different ecotopes within the rural area
showed the highest frequencies of Ev. bourrouli and Ny.
antunesi in chicken coops, whereas Ny. whitmani
predominated in this ecotope in the periurban area. In the rural settlement
area, Ev. bourrouli was the most frequently captured species in
automatic light traps and Ps. complexus was the most prevalent
in Shannon trap captures. The rural settlement environment exhibited greater
phlebotomine biodiversity than the periurban area. Ps.
complexus and Psychodopygus ayrozai naturally
infected with Leishmania (Viannia) braziliensis were
identified. The data identified Ny. whitmani as a potential ACL
vector in the periurban area, whereas Ps. complexus was more
prevalent in the rural environment associated with settlements. 相似文献
30.
Maria?B.?FonsecaEmail author Jhonathan?O.?Silva Luiz?A.?D.?Falc?o Mariana?G.?V.?Dupin Geraldo?A.?Melo Mário?M.?Espírito-Santo 《Plant Ecology》2018,219(4):403-415
Herbivory has significant impacts on individual plants and plant communities, both at ecological and evolutionary time scales. In this context, this study aims to evaluate herbivore damage and its relationship with leaf chemical and structural traits, nutritional status, and forest structural complexity along a successional gradient. We predicted that trees in early successional stages support conservative traits related to drought tolerance (high specific leaf mass and phenolics), whereas trees in light-limited, late successional stages tend to enhance light acquisition strategies (high nitrogen content). We sampled 261 trees from 26 species in 15 plots (50 × 20 m; five per successional stage). From each tree, twenty leaves were collected for leaf trait measures. Phenolic content increased whereas specific leaf mass and nitrogen content decreased from early to late stages. However, leaf damage did not differ among successional stages. Our results partially corroborate the hypothesis that early successional plants in tropical dry forests exhibit leaf traits involved in the conservative use of water. The unexpected decrease in nitrogen content along the chronosequence is likely related to the fact that thinner leaves with low specific leaf mass could have less nitrogen-containing mesophyll per unit area. Mechanisms affecting herbivory intensity varied across scales: at the species level, leaf damage was negatively correlated with tannin concentration and specific leaf mass; at the plot level, leaf damage was positively affected by forest structural complexity. Herbivory patterns in tropical forests are difficult to detect because abiotic factors and multiple top-down and bottom-up forces directly and indirectly affect herbivores. 相似文献