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161.
162.
Martha Robles-Flores Erika Rendon-Huerta Hector Gonzalez-Aguilar Guillermo Mendoza-Hernandez Socorro Islas Valentin Mendoza M Veronica Ponce-Castaneda Lorenza Gonzalez-Mariscal Fernando Lopez-Casillas 《The Journal of biological chemistry》2002,277(7):5247-5255
The aim of this study was to identify cellular proteins that bind protein kinase C (PKC) and may influence its activity and its localization. A 32-kDa PKC-binding protein was purified to homogeneity from the Triton X-100-insoluble fraction obtained from hepatocytes homogenates. The protein was identified by NH(2)-terminal amino acid sequencing as the previously described mature form of p32 (gC1qR). Recombinant p32 was expressed as a glutathione S-transferase fusion protein, affinity-purified, and tested for an in vitro interaction with PKC using an overlay assay approach. All PKC isoforms expressed in rat hepatocytes interacted in vitro with p32, but the binding dependence on PKC activators was different for each one. Whereas PKCdelta only binds to p32 in the presence of PKC activators, PKCzeta and PKCalpha increase their binding when they are in the activated form. Other PKC isoforms such as beta, epsilon, and theta bind equally well to p32 regardless of the presence of PKC activators, and PKCmu binds even better in their absence. It was also found that p32 is not a substrate for any of the PKC isoforms tested, but interestingly, its presence had a stimulatory effect (2-fold for PKCdelta) on PKC activity. We also observed in vivo interaction between PKC and p32 by immunofluorescence and confocal microscopy. A time course of phorbol ester treatment of cultured rat hepatocytes (C9 cells) showed that PKCtheta and p32 are constitutively associated in vivo, whereas PKCdelta activation is required for its association with p32. Our data also showed that phorbol ester treatment induces a transient translocation of p32 from the cytoplasm to the cell nucleus. Together, these findings suggest that p32 may be a regulator of PKC location and function. 相似文献
163.
164.
Hector E. Flores Paula J. Sgrignoli 《In vitro cellular & developmental biology. Plant》1991,27(3):139-142
Summary The lengthy dormancy requirement of yew seeds can be overcome with a simple in vitro method. Viable embryos were excised from
seeds ofTaxus brevifolia and four cultivars ofT. media over a range of developmental stages. Embryos were cultured in several basal media formulations (Whites’, Gamborg’s B5 and
Murashige and Skoog’s) under dark or light. After a lag period of 1 to 2 wk, embryos of both species germinated precociously.
Germination rates of up to 70% were obtained withT. media cv. Hicksi embryos. The highest rates of germination were obtained in White’s and MS media. Embryos excised from green seeds
with undeveloped arils showed the highest germination rates. As the seeds approached maturity, in vitro germination rates
of the excised embryos declined dramatically. Green seeds and seeds with developing arils could be stored at 5° C without
large loss in embryo germination. Seeds with fully developed arils could be stored frozen at −20° C for 1 wk while still allowing
about 50% of embryo germination. At least 30% of the precociously germinated embryos of both species were able to develop
into full seedlings. Our method appears to be generally applicable toTaxus spp.
This research was supported by a grant from the Hawaii Biotechnology Group, Inc. 相似文献
165.
Jonathan Massey Simon Rothwell Clare Rusbridge Anna Tauro Diane Addicott Hector Chinoy Robert G. Cooper William E. R. Ollier Lorna J. Kennedy 《PloS one》2013,8(2)
A breed-specific polymyositis is frequently observed in the Hungarian Vizsla. Beneficial clinical response to immunosuppressive therapies has been demonstrated which points to an immune-mediated aetiology. Canine inflammatory myopathies share clinical and histological similarities with the human immune-mediated myopathies. As MHC class II associations have been reported in the human conditions we investigated whether an MHC class II association was present in the canine myopathy seen in this breed. 212 Hungarian Vizsla pedigree dogs were stratified both on disease status and degree of relatedness to an affected dog. This generated a group of 29 cases and 183 “graded” controls: 93 unaffected dogs with a first degree affected relative, 44 unaffected dogs with a second degree affected relative, and 46 unaffected dogs with no known affected relatives. Eleven DLA class II haplotypes were identified, of which, DLA-DRB1*02001/DQA1*00401/DQB1*01303, was at significantly raised frequency in cases compared to controls (OR = 1.92, p = 0.032). When only control dogs with no family history of the disease were compared to cases, the association was further strengthened (OR = 4.08, p = 0.00011). Additionally, a single copy of the risk haplotype was sufficient to increase disease risk, with the risk substantially increasing for homozygotes. There was a trend of increasing frequency of this haplotype with degree of relatedness, indicating low disease penetrance. These findings support the hypothesis of an immune-mediated aetiology for this canine myopathy and give credibility to potentially using the Hungarian Vizsla as a genetic model for comparative studies with human myositis. 相似文献
166.
167.
Carsten Schwarz Dominik Hartl Olaf Eickmeier Andreas Hector Christian Benden Isabelle Durieu Amparo Sole Silvia Gartner Carlos E. Milla Peter James Barry 《Mycopathologia》2018,183(1):21-32
Cystic fibrosis (CF) is a chronic lethal multi-system condition; however, most of the morbidity and mortality is dependent on the status of the respiratory system. Progressive respiratory decline is mediated by chronic infection and inflammation, punctuated by important acute events known as pulmonary exacerbations which can lead to accelerated decline. The main bacterial species causing infections include Pseudomonas aeruginosa, Staphylococcus aureus, Haemophilus influenzae and Achromobacter xylosoxidans. In addition to bacteria, fungi are detected in a significant number of patients. The impact of fungal colonization of the airways is still not completely elucidated, but an increasing body of evidence suggests an important role for moulds and yeasts. Although fungal infections are rare, fungi can cause severe pneumonia requiring appropriate targeted treatment. The most common fungi in respiratory samples of patients with CF are Aspergillus fumigatus, Aspergillus terreus and Scedosporium species for filamentous fungi, and yeasts such as Candida albicans and Candida glabrata. Therapeutic strategies depend on the detected fungus and the underlying clinical status of the patient. The antifungal therapy can range from a simple monotherapy up to a combination of three different drugs. Treatment course may be indicated in some patients for two weeks and in others for up to six months, and in rare cases even longer. New antifungal drugs have been developed and are being tested in clinical studies offering the hope of therapeutic alternatives to existing drugs. Identifying relevant risk factors and diagnostic criteria for fungal colonization and infection is crucial to enabling an adequate prevention, diagnosis and treatment. 相似文献
168.
Coral Reefs - Both natural and anthropogenic factors are changing coral-reef structure and function worldwide. Long-term monitoring has revealed declines in the local composition and species... 相似文献
169.
Impact of long‐term chromosomal shuffling on the multispecies coalescent analysis of two anthropoid primate lineages 下载免费PDF全文
Carlos G. Schrago Beatriz Mello Anieli G. Pereira Carolina Furtado Hector N. Seuánez 《Ecology and evolution》2018,8(2):1206-1216
Multispecies coalescent (MSC) theory assumes that gene trees inferred from individual loci are independent trials of the MSC process. As genes might be physically close in syntenic associations spanning along chromosome regions, these assumptions might be flawed in evolutionary lineages with substantial karyotypic shuffling. Neotropical primates (NP) represent an ideal case for assessing the performance of MSC methods in such scenarios because chromosome diploid number varies significantly in this lineage. To this end, we investigated the effect of sequence length on the theoretical expectations of MSC model, as well as the results of coalescent‐based tree inference methods. This was carried out by comparing NP with hominids, a lineage in which chromosome macrostructure has been stable for at least 15 million years. We found that departure from the MSC model in Neotropical primates decreased with smaller sequence fragments, where sites sharing the same evolutionary history were more frequently found than in longer fragments. This scenario probably resulted from extensive karyotypic rearrangement occurring during the radiation of NP, contrary to the comparatively stable chromosome evolution in hominids. 相似文献
170.
David G. Cameron Hector L. Casal Henry H. Mantsch 《Journal of biochemical and biophysical methods》1979,1(1):21-36
Fourier transform infrared transmission spectroscopy is presented as a technique with great potential for the study of aqueous membrane preparations. The methodology of sample preparation, spectra recording and data reduction is outlined. Spectral parameters are derived from FT-IR difference spectra which provide an extremely sensitive means to monitor the temperature-dependent behavior of individual vibrational modes in model and natural membranes. 相似文献