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排序方式: 共有181条查询结果,搜索用时 15 毫秒
91.
Alexis S. Ivanov Alexei Medvedev Pavel Ershov Andrey Molnar Yury Mezentsev Evgeny Yablokov Leonid Kaluzhsky Oksana Gnedenko Olga Buneeva Irina Haidukevich Gennadiy Sergeev Aliaksandr Lushchyk Alexey Yantsevich Marina Medvedeva Sergey Kozin Igor Popov Svetlana Novikova Victor Zgoda Andrey Gilep Sergey Usanov Andrey Lisitsa Alexander Archakov 《Proteomics》2014,14(20):2261-2274
There is increasing evidence that proteins function in the cell as integrated stable or temporally formed protein complexes, interactomes. Previously, using model systems we demonstrated applicability of direct molecular fishing on paramagnetic particles for protein interactomics (Ershov et al. Proteomics, 2012, 12, 3295). In the present study, we have used a combination of affinity‐based molecular fishing and subsequent MS for investigation of human liver proteins involved in interactions with immobilized microsomal cytochrome b5 (CYB5A), and also transthyretin and BSA as alternative affinity ligands (baits). The LC?MS/MS identification of prey proteins fished on these baits revealed three sets of proteins: 98, 120, and 220, respectively. Comparison analysis of these sets revealed only three proteins common for all the baits. In the case of paired analysis, the number of common proteins varied from 2 to 9. The binding capacity of some identified proteins has been validated by a SPR‐based biosensor. All the investigated proteins effectively interacted with the immobilized CYB5A (Kd values ranged from 0.07 to 1.1 μM). Results of this study suggest that direct molecular fishing is applicable for analysis of protein–protein interactions (PPI) under normal and pathological conditions, in which altered PPIs are especially important. 相似文献
92.
N. V. Zavyalov V. V. Maslov V. G. Rumyantsev I. Yu. Drozdov D. A. Ershov D. S. Korkin D. A. Molodtsev V. I. Smerdov A. P. Falin A. A. Yukhimchuk 《Plasma Physics Reports》2013,39(3):243-247
Results from preliminary experimental research of neutron emission generated by a spherical plasma focus chamber filled with an equal-component deuterium-tritium mixture are presented. At a maximum current amplitude in the discharge chamber of ~1.5 MA, neutron pulses with a full width at half-maximum of 75–80 ns and an integral yield of ~1.3 × 1013 DT neutrons have been recorded. 相似文献
93.
94.
Inactivation of sll1556 in Synechocystis strain PCC 6803 impairs isoprenoid biosynthesis from pentose phosphate cycle substrates in vitro 下载免费PDF全文
Poliquin K Ershov YV Cunningham FX Woreta TT Gantt RR Gantt E 《Journal of bacteriology》2004,186(14):4685-4693
In cyanobacteria many compounds, including chlorophylls, carotenoids, and hopanoids, are synthesized from the isoprenoid precursors isopentenyl diphosphate (IPP) and dimethylallyl diphosphate. Isoprenoid biosynthesis in extracts of the cyanobacterium Synechocystis strain PCC 6803 grown under photosynthetic conditions, stimulated by pentose phosphate cycle substrates, does not appear to require methylerythritol phosphate pathway intermediates. The sll1556 gene, distantly related to type 2 IPP isomerase genes, was disrupted by insertion of a Kanr cassette. The mutant was fully viable under photosynthetic conditions although impaired in the utilization of pentose phosphate cycle substrates. Compared to the parental strain the Deltasll1556 mutant (i) is deficient in isoprenoid biosynthesis in vitro with substrates including glyceraldehyde-3-phosphate, fructose-6-phosphate, and glucose-6-phosphate; (ii) has smaller cells (diameter ca. 13% less); (iii) has fewer thylakoids (ca. 30% less); and (iv) has a more extensive fibrous outer wall layer. Isoprenoid biosynthesis is restored with pentose phosphate cycle substrates plus the recombinant Sll1556 protein in the Deltasll1556 supernatant fraction. IPP isomerase activity could not be demonstrated for the purified Sll1556 protein under our in vitro conditions. The reduction of thylakoid area and the effect on outer wall layer components are consistent with an impairment of isoprenoid biosynthesis in the mutant, possibly via hopanoid biosynthesis. Our findings are consistent with an alternate metabolic shunt for biosynthesis of isoprenoids. 相似文献
95.
Ugriumov MV Mel'nikova VI Ershov PV Balan I Calas A 《Rossi?skii fiziologicheski? zhurnal imeni I.M. Sechenova / Rossi?skaia akademiia nauk》2000,86(10):1255-1267
The study has evaluated in vivo, ex vivo and in vitro ontogenesis and functional significance of the arcuate nucleus neurons expressing either individual enzymes of dopamine synthesis, tyrosine hydroxylase or aromatic L-amino acid decarboxylase as well as both of them (dopaminergic neurons) in rats from the 17th embryonic day to adulthood. Monoenzymatic tyrosine hydroxylase-containing neurons were initially observed on the 18th embryonic day. On the 20-21 day, the monoenzymatic tyrosine hydroxylase- or aromatic L-amino acid decarboxylase-expressing neurons comprised more than 99% of the whole neuron population expressing the dopamine-synthesizing enzymes. The dopamine production in the fetus arcuate nucleus was sufficient to provide an inhibitory control of prolactin secretion like in adults. The data suggest a possibility of the dopamine synthesis in the fetus arcuate nucleus by the monoenzymatic neurons containing either tyrosine hydroxylase or aromatic L-amino acid decarboxylase-expressing neurons in co-operation. 相似文献
96.
A. D. Ryabov Yulia N. Firsova Aleksei Y. Ershov Ilia A. Dementiev 《Journal of biological inorganic chemistry》1999,4(2):175-182
Glucose oxidase-catalyzed reduction of cis[MIII (LL)2Cl2]+ (M=Os and Ru) complexes to cis[MII (LL)2Cl2] (LL=2,2′-bipyridine and 1,10-phenanthroline type ligands) by d-glucose is a first-order process in the complex and the enzyme in aqueous buffered solution. The reaction follows MichaelisMenten
kinetics in d-glucose and the rate is independent of d-glucose concentration above 0.03 M. The reactivity decreases in the series [Ru(bpy)2Cl2]+ > [Os(phen)2Cl2]+ > [Os(4,4′-Me2bpy)2Cl2]+ > [Os(4,7Me2phen)2Cl2]+. The measured second-order rate constant for the oxidation of reduced glucose oxidase by [Os(phen)2Cl2]+ in air equals 1.2×105 M–1 s–1 at pH 6.7, [d-glucose] 0.05 M, and 25 °C, which is ca. 20% less than that when the reaction solutions are purged with argon. In the case
of [Ru(bpy)2Cl2]+ the rate constant equals 1.8×105 M–1 s–1 under similar conditions in air, showing higher reactivity of Ru complexes compared with Os ones. The reduction is pH-dependent
with a maximum around 7. Added for solubilization of poorly soluble metal complexes, surfactants decrease the rates of the
enzymatic reaction. The retardation effect increases in the series: cetyltrimethylammonium bromide < Triton X-100 < sodium
dodecyl sulfate, i.e. on going from positively charged to neutral and then to negatively charged surfactants. The behavior
of the OsIII and RuIII complexes toward reduced glucose oxidase contrasts to that of recently studied ferricenium cations. As opposed to the latter,
the former do not show kinetically meaningful binding with the enzyme, and the Michaelis kinetics typical of the ferricenium
case is not realized for the OsIII, and RuIII species. The systems OsIII- or RuIII-glucose oxidase are convenient for routine "one pot" spectrophotometric monitoring of the d-glucose content in samples, since the metal reduction to MII is accompanied by a strong increase in absorbance in the visible spectral region.
Received: 1 July 1998 / Accepted: 13 January 1999 相似文献
97.
T M Sokolova N P Bugaeva I A Suetina F I Ershov E S Ketiladze 《Antibiotiki i khimioterapii͡a》1989,34(6):472-475
Treatment of patients suffering from chronic hepatitis B with recombinant leukocytic interferon (reaferon) increased the levels of circulating interferon and activated interferon-dependent enzymes such as 2-5A-synthetase and histone kinase. Activation of the enzymes was observed for 1 to 2 weeks. After that period it was maintained at the required levels with intramuscular administration of 1-3 million units of reaferon 2 or 3 times a week. In parallel with increasing of the levels of the interferon system enzymes there was observed a decrease in the level of aminotransferase. The reaction of the viral antigens to the treatment with reaferon was not the same: HBe antigen and antibodies to HBe antigen disappeared, the content of HBs antigen and antibodies to delta-interferon did not change. 相似文献
98.
99.
The results of study on 2 Soviet interferon inductors, i.e. the synthetic polyguacyl polynucleotide and natural double-strand phage RNA or dsRNA were studied. It was shown that the time course of accumulation and period of circulation of interferon depended on the route of the inductor administration. The antiviral activity of polyguacyl and dsRNA in experimental influenza and tick-borne encephalitis is described. The maximum protective effect with respect to experimental influenza was observed with intranasal administration of the drugs 4 hours before inoculation. A pronounced protective effect with respect to tick-borne encephalitis was observed with intraperitoneal administration of the inductors or their use in the form of aerosols. Direct correlation between interferon production and the final protective effect was found. 相似文献
100.
A theory of the behavior of biological systems is proposed which is an extension of the conception of biological evolution (Gladyshev, 1977, Gladyshev, 1978) based on classical (equilibrium) thermodynamics. A thermodynamic theory of homeostasis is presented, in accordance with which homeostatic mechanisms of regulation are connected with a compensative shift of a fundamental quasi-equilibrium. The principle of least compulsion is formulated on the basis of thermodynamic laws and describes behavior of biological systems. A fundamental thermodynamic equation of behavioral processes is introduced. The Weber-Fechner law is shown to be a corollary of the fundamental thermodynamic equation. 相似文献