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71.
S B Cheknev A N Narovlianski? A M Amchenkova A M Sorokin Ia E Khesin F I Ershov 《Biulleten' eksperimental'no? biologii i meditsiny》1990,110(10):406-409
The cytotoxic activity of peripheral blood natural killers (NK) against target cells (TC) J-96 and L-929 with high sensitivity to interferon (IFN) action, J-41 and MCB resistant to IFN action and line K-562 labelled by H3-uridine was studied in 14 hrs cytotoxic test. It has been shown that human TC J-96 didn't differ from the J-41 in their sensitivity to NK cytotoxicity and they are strongly resistant to NK than TC K-562. The murine TC L-929 as the human TC didn't differ from the MCB in their sensitivity to NK lysis and had also the same sensitivity to NK as the K-562 cells. 相似文献
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Shibkov V. M. Aleksandrov A. F. Ershov A. P. Timofeev I. B. Chernikov V. A. Shibkova L. V. 《Plasma Physics Reports》2005,31(9):795-801
Plasma Physics Reports - A discharge produced by a focused microwave beam in a supersonic gas flow has been investigated experimentally. It is shown that the degree of ionization and the gas... 相似文献
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T. N. Nazina D. Sh. Sokolova T. L. Babich E. M. Semenova A. P. Ershov S. Kh. Bidzhieva I. A. Borzenkov A. B. Poltaraus M. R. Khisametdinov T. P. Tourova 《Microbiology》2017,86(6):773-785
Physicochemical and microbiological characteristics of formation waters low-temperature heavy oil reservoirs (Russia) were investigated. The Chernoozerskoe, Yuzhno-Suncheleevskoe, and Severo-Bogemskoe oilfields, which were exploited without water-flooding, were shown to harbor scant microbial communities, while microbial numbers in the water-flooded strata of the Vostochno-Anzirskoe and Cheremukhovskoe oilfields was as high as 106 cells/mL. The rates of sulfate reduction and methanogenesis were low, not exceeding 1982 ng S2–/(L day) and 9045 nL СН4/(L day), respectively, in the samples from water-flooded strata. High-throughput sequencing of microbial 16S rRNA gene fragments in the community of injection water revealed the sequences of the Proteobacteria (74.7%), including Betaproteobacteria (40.2%), Alphaproteobacteria (20.7%), Gammaproteobacteria (10.1%), Deltaproteobacteria (2.0%), and Epsilonproteobacteria (1.6%), as well as Firmicutes (7.9%), Bacteroidetes (4.1%), and Archaea (0.2%). DGGE analysis of microbial mcrA genes in the community of injection water revealed methanogens of the genera Methanothrix, Methanospirillum, Methanobacterium, Methanoregula, Methanosarcina, and Methanoculleus, as well as unidentified Thermoplasmata. Pure cultures of bacteria of the genera Rhodococcus, Pseudomonas, Gordonia, Cellulomonas, etc., capable of biosurfactant production when grown on heavy oil, were isolated. Enrichment cultures of fermentative bacteria producing significant amounts of volatile organic acids (acetic, propionic, and butyric) from sacchariferous substrates were obtained. These acids dissolve the carbonates of oil-bearing rock efficiently. Selection of the efficient microbial technology for enhanced recovery of heavy oil from terrigenous and carbonate strata requires model experiments with microbial isolates and the cores of oil-bearing rocks. 相似文献
78.
Ugrumov M Pronina T Ershov P Wittkowski W Gharib C Gabrion J Grigoriev A 《Journal of gravitational physiology : a journal of the International Society for Gravitational Physiology》2002,9(1):P45-P46
This study was aimed to evaluate the reaction of the vasopressin (VP) and oxytocin (OT) neurons of the supraoptic nucleus (SON) in rats to single or repeated hypergravity (HG). Special attention was paid to the tyrosine hydroxylase (TH) expression in VP neurons as a marker of the neuron activation. Rats were revolved in a centrifuge with overloading 2G for 5 days or 34 days as well as for 34 days plus 5 days with an interval of 39 days between two rotations. Control rats were kept in a centrifuge room. Radioimmununoassay, quantitative and semi-quantitative immunocytochemistry and in situ hybridization were used to evaluate: a) VP concentration in the pituitary posterior lobe (PL) and in plasma; b) the number of VP-, OT- and TH-immunoreactive neurons in the SON; c) the optic density of VP-, OT- and TH-immunoreactive materials in cell bodies (SON) and distal axons (PL), d) the optic density of VP and OT mRNAs signals (S35) in the whole SON on microfilms. According to our data, VP neurons were strongly activated during HG (5 days or 34 days) that was manifested in the functional hypertrophy of the neurons, greatly increased concentrations of VP mRNA in the SON and VP in plasma, the onset of the TH expression. The neurons showed initially (5 days) the functional insufficiency (VP release > VP synthesis) followed by their adaptation (subsequent 29 days) to the increased need in VP (VP release < VP synthesis). No reaction of VP neurons was observed to repeated HG. In contrast to VP neurons, OT neurons did not react to short-term HG or showed functional depression after the long-term treatment. 相似文献
79.
Ivanova EV Ershov AY Laurinavicius V Meskus R Ryabov AD 《Biochemistry. Biokhimii?a》2003,68(4):407-415
The comparative kinetic study of two glucose oxidizing enzymes, FAD-dependent glucose oxidase and PQQ-dependent glucose dehydrogenase, is presented in the artificial electron transfer mediator system based on ruthenium(III) compounds. It is demonstrated that FAD-dependent glucose oxidase and PQQ-dependent glucose dehydrogenase follow Michaelis kinetics in the D-glucose/ruthenium(III) system. PQQ-dependent glucose dehydrogenase is more active than FAD-dependent glucose oxidase in the process of D-glucose oxidation by ruthenium(III) compounds, this being due to the different catalytic mechanisms of these enzymes. 相似文献
80.
Ershov FI 《Zhurnal mikrobiologii, epidemiologii, i immunobiologii》2007,(4):100-103
Drugs that recently (last 2-3 years) have been widely used for prevention of the most prevalent and still imperfectly controlled viral infections. Special attention was attended to use of interferons and other nonspecific cytokines as main factors of innate immunity as well as to new inducers of endogenous interferon (kagocel, allokyn). Conclusion was made that under the conditions of availabilityof wide spectrum of antiviral drugs, their clinical effectiveness is determined by scientifically founded algorithm of their use. 相似文献