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991.
In many developing nations, “paper parks”, or protected areas that have little or no formal management on the ground, have
resulted from the failure of protected area systems to achieve their foremost goal: biodiversity conservation. This analysis
incorporates biophysical, socioeconomic, and land use/tenure data collected by a multi-disciplinary team of Guatemalan and
American researchers in order to identify potential management plans and multiple-use/concession arrangements. The Sierra
Chinajá is a classic paper park protected area in Guatemala. Many factors have rendered Guatemalan protected areas management
policies ineffectual in the Sierra Chinajá despite the fact that it has been an “area of special protection” since 1989. Proximate
causes of forest conversion mask underlying driving forces responsible for rapid biodiversity loss. Despite the fact that
Guatemala’s protected areas management system is similar to that promoted by international conservation organizations it has
yet to effectively conserve biodiversity. These factors suggest that protected areas management in Guatemala, and other developing
nations possessing unique cultural and natural histories, must be rooted in the local context as promulgated by the local
non-governmental organization ProPéten in their proposal for an official Indigenous Reserve category. The proposal suggests
the devolution of management responsibilities from federal institutions to local communities in the effort to develop a community-based,
site specific conservation agenda.
Resumen En muchos países en desarrollo, las “áreas protegidas en papel” o parques que no poseen un plan de manejo formal, han sido el resultado de la incapacidad del sistema nacional de áreas protegidas de alcanzar su meta mas importante: la conservación de la biodiversidad. El siguiente análisis incorpora datos biofísicos, socioeconómicos, de uso y tenencia de la tierra recolectados por un grupo multidisciplinario de investigadores guatemaltecos y norteamericanos con el objetivo de formular una estrategia de conservación que incorpore concesiones de usos múltiples. La Sierra de Chinajá es un ejemplo clásico de un “área protegida en papel” en Guatemala. Muchos son los factores por los cuales la política de áreas protegidas ha fracasado en la Sierra de Chinajá, a pesar de estar clasificada como un “área de Protección Especial” desde 1989. Las causas subyacentes responsables por el cambio en la cobertura forestal están escondidas debajo de los síntomas más visibles de la perdida de biodiversidad. A pesar de que el Sistema Guatemalteco de áreas Protegidas es similar al promovido por organizaciones internacionales aun no es efectivo en la conservación de la biodiversidad. Estos factores sugieren que el manejo de las áreas protegidas en Guatemala, y en otros países en desarrollo que poseen historias naturales y culturales únicas, deben estar enraizadas en el contexto local, como ha sido propuesto por la organización Pro-Peten en su propuesta por una categoría de manejo denominada Reservas Comunitarias Indígenas. La propuesta sugiere la delegación de la protección la biodiversidad de instituciones estatales a las comunidades locales con el propósito de establecer una agenda de conservación basada en el manejo comunitario.相似文献
992.
Iwona Ruchala Vanessa Cabra Ernesto Solis Jr. Richard A. Glennon Louis J. De Felice Jose M. Eltit 《Cell calcium》2014
Monoamine transporters have been implicated in dopamine or serotonin release in response to abused drugs such as methamphetamine or ecstasy (MDMA). In addition, monoamine transporters show substrate-induced inward currents that may modulate excitability and Ca2+ mobilization, which could also contribute to neurotransmitter release. How monoamine transporters modulate Ca2+ permeability is currently unknown. We investigate the functional interaction between the human serotonin transporter (hSERT) and voltage-gated Ca2+ channels (CaV). We introduce an excitable expression system consisting of cultured muscle cells genetically engineered to express hSERT. Both 5HT and S(+)MDMA depolarize these cells and activate the excitation-contraction (EC)-coupling mechanism. However, hSERT substrates fail to activate EC-coupling in CaV1.1-null muscle cells, thus implicating Ca2+ channels. CaV1.3 and CaV2.2 channels are natively expressed in neurons. When these channels are co-expressed with hSERT in HEK293T cells, only cells expressing the lower-threshold L-type CaV1.3 channel show Ca2+ transients evoked by 5HT or S(+)MDMA. In addition, the electrical coupling between hSERT and CaV1.3 takes place at physiological 5HT concentrations. The electrical coupling between monoamine neurotransmitter transporters and Ca2+ channels such as CaV1.3 is a novel mechanism by which endogenous substrates (neurotransmitters) or exogenous substrates (like ecstasy) could modulate Ca2+-driven signals in excitable cells. 相似文献
993.
Sonia Gabriela Ortiz‐Maciel Alejandro Salinas‐Melgoza Simón Octavio Valdéz‐Juárez Leonel Lopez‐Toledo Ernesto Enkerlin‐Hoeflich 《Ibis》2014,156(2):299-310
Stochastic and catastrophic events may strongly impact the dynamics of wild populations. Annual fluctuations in rainfall may affect parrot populations, but few studies address the impact of other stochastic or catastrophic events on their population dynamics. The Maroon‐fronted Parrot Rhynchopsitta terrisi is an endangered species that nests colonially in cavities and crevices in limestone cliffs. From 1995 to 2010, we quantified Parrot attendance at nesting colonies throughout its breeding range, and reproductive output of nesting Parrots from 1997 to 2010 at the two most important nesting colonies. There was significant variation among colonies in the number of cavities occupied by Parrots each year. Rainfall significantly influenced both the number of occupied cavities and productivity, which declined after very dry years. Natural unpredictable events such as hurricanes did not modify the nesting activity of Maroon‐fronted Parrots at breeding colonies. However, wildfires increased in dry years, negatively affecting attendance at breeding colonies. The Maroon‐fronted Parrot may overcome the impacts of climatic variability, natural stochastic processes, and human‐induced catastrophic events by using nesting colonies as a network of resources throughout the breeding range. Given the current trends in climate change, it is likely the species may suffer stronger and more frequent unpredictable catastrophic events, potentially putting at risk its survival in the long term. 相似文献
994.
Cavieres LA Badano EI Sierra-Almeida A Gómez-González S Molina-Montenegro MA 《The New phytologist》2006,169(1):59-69
In alpine habitats, positive interactions among plants tend to increase with elevation as a result of altitudinal increase in environmental harshness. However, in mountains located in arid zones, lower elevations are also stressful because of scarce availability of water, suggesting that positive interactions may not necessarily increase with elevation. Here we analysed the spatial association of plant species with the nurse cushion plant Laretia acaulis at two contrasting elevations, and monitored the survival of seedlings of two species experimentally planted within and outside cushions in the semiarid Andes of central Chile. Positive spatial associations with cushions were more frequent at lower elevations. Species growing at the two elevations changed the nature of their association with cushions from neutral or negative at higher elevations to positive at lower elevations. Survival of seedlings was higher within cushions, particularly at lower elevations. The increased facilitation by cushions at lower elevations seems to be related to provision of moisture. This result suggests that cushion plants play a critical role in structuring alpine plant communities at lower elevations, and that climatic changes in rainfall could be very relevant for persistence of plant communities. 相似文献
995.
996.
Fluorescence optical detection in sedimentation velocity analytical ultracentrifugation allows the study of macromolecules at nanomolar concentrations and below. This has significant promise, for example, for the study of systems of high-affinity protein interactions. Here we describe adaptations of the direct boundary modeling analysis approach implemented in the software SEDFIT that were developed to accommodate unique characteristics of the confocal fluorescence detection system. These include spatial gradients of signal intensity due to scanner movements out of the plane of rotation, temporal intensity drifts due to instability of the laser and fluorophores, and masking of the finite excitation and detection cone by the sample holder. In an extensive series of experiments with enhanced green fluorescent protein ranging from low nanomolar to low micromolar concentrations, we show that the experimental data provide sufficient information to determine the parameters required for first-order approximation of the impact of these effects on the recorded data. Systematic deviations of fluorescence optical sedimentation velocity data analyzed using conventional sedimentation models developed for absorbance and interference optics are largely removed after these adaptations, resulting in excellent fits that highlight the high precision of fluorescence sedimentation velocity data, thus allowing a more detailed quantitative interpretation of the signal boundaries that is otherwise not possible for this system. 相似文献
997.
Anna Grazia Recchia Nadia Caruso Sabrina Bossio Mariavaleria Pellicanò Laura De Stefano Stefania Franzese Angela Palummo Vincenzo Abbadessa Eugenio Lucia Massimo Gentile Ernesto Vigna Clementina Caracciolo Antolino Agostino Sara Galimberti Luciano Levato Fabio Stagno Stefano Molica Bruno Martino Paolo Vigneri Francesco Di Raimondo Fortunato Morabito 《PloS one》2015,10(6)
998.
Israel S. Fernández Akio Yamashita Ernesto Arias-Palomo Yumi Bamba Ruben A. Bartolomé M. Angeles Canales Joaquín Teixidó Shigeo Ohno Oscar Llorca 《Nucleic acids research》2011,39(1):347-358
SMG-9 is part of a protein kinase complex, SMG1C, which consists of the SMG-1 kinase, SMG-8 and SMG-9. SMG1C mediated phosphorylation of Upf1 triggers nonsense-mediated mRNA decay (NMD), a eukaryotic surveillance pathway that detects and targets for degradation mRNAs harboring premature translation termination codons. Here, we have characterized SMG-9, showing that it comprises an N-terminal 180 residue intrinsically disordered region (IDR) followed by a well-folded C-terminal domain. Both domains are required for SMG-1 binding and the integrity of the SMG1C complex, whereas the C-terminus is sufficient to interact with SMG-8. In addition, we have found that SMG-9 assembles in vivo into SMG-9:SMG-9 and, most likely, SMG-8:SMG-9 complexes that are not constituents of SMG1C. SMG-9 self-association is driven by interactions between the C-terminal domains and surprisingly, some SMG-9 oligomers are completely devoid of SMG-1 and SMG-8. We propose that SMG-9 has biological functions beyond SMG1C, as part of distinct SMG-9-containing complexes. Some of these complexes may function as intermediates potentially regulating SMG1C assembly, tuning the activity of SMG-1 with the NMD machinery. The structural malleability of IDRs could facilitate the transit of SMG-9 through several macromolecular complexes. 相似文献
999.
Background and Aims
While the climbing habit allows vines to reach well-lit canopy areas with a minimum investment in support biomass, many of them have to survive under the dim understorey light during certain stages of their life cycle. But, if the growth/survival trade-off widely reported for trees hold for climbing plants, they cannot maximize both light-interception efficiency and shade avoidance (i.e. escaping from the understorey). The seven most important woody climbers occurring in a Chilean temperate evergreen rainforest were studied with the hypothesis that light-capture efficiency of climbers would be positively associated with their abundance in the understorey.Methods
Species abundance in the understorey was quantified from their relative frequency and density in field plots, the light environment was quantified by hemispherical photography, the photosynthetic response to light was measured with portable gas-exchange analyser, and the whole shoot light-interception efficiency and carbon gain was estimated with the 3-D computer model Y-plant.Key Results
Species differed in specific leaf area, leaf mass fraction, above ground leaf area ratio, light-interception efficiency and potential carbon gain. Abundance of species in the understorey was related to whole shoot features but not to leaf level features such as specific leaf area. Potential carbon gain was inversely related to light-interception efficiency. Mutual shading among leaves within a shoot was very low (<20 %).Conclusions
The abundance of climbing plants in this southern rainforest understorey was directly related to their capacity to intercept light efficiently but not to their potential carbon gain. The most abundant climbers in this ecosystem match well with a shade-tolerance syndrome in contrast to the pioneer-like nature of climbers observed in tropical studies. The climbers studied seem to sacrifice high-light searching for coping with the dim understorey light. 相似文献1000.
Genomic analysis of the necrotrophic fungal pathogens Sclerotinia sclerotiorum and Botrytis cinerea 总被引:2,自引:0,他引:2
Amselem J Cuomo CA van Kan JA Viaud M Benito EP Couloux A Coutinho PM de Vries RP Dyer PS Fillinger S Fournier E Gout L Hahn M Kohn L Lapalu N Plummer KM Pradier JM Quévillon E Sharon A Simon A ten Have A Tudzynski B Tudzynski P Wincker P Andrew M Anthouard V Beever RE Beffa R Benoit I Bouzid O Brault B Chen Z Choquer M Collémare J Cotton P Danchin EG Da Silva C Gautier A Giraud C Giraud T Gonzalez C Grossetete S Güldener U Henrissat B Howlett BJ Kodira C Kretschmer M Lappartient A Leroch M 《PLoS genetics》2011,7(8):e1002230
Sclerotinia sclerotiorum and Botrytis cinerea are closely related necrotrophic plant pathogenic fungi notable for their wide host ranges and environmental persistence. These attributes have made these species models for understanding the complexity of necrotrophic, broad host-range pathogenicity. Despite their similarities, the two species differ in mating behaviour and the ability to produce asexual spores. We have sequenced the genomes of one strain of S. sclerotiorum and two strains of B. cinerea. The comparative analysis of these genomes relative to one another and to other sequenced fungal genomes is provided here. Their 38-39 Mb genomes include 11,860-14,270 predicted genes, which share 83% amino acid identity on average between the two species. We have mapped the S. sclerotiorum assembly to 16 chromosomes and found large-scale co-linearity with the B. cinerea genomes. Seven percent of the S. sclerotiorum genome comprises transposable elements compared to <1% of B. cinerea. The arsenal of genes associated with necrotrophic processes is similar between the species, including genes involved in plant cell wall degradation and oxalic acid production. Analysis of secondary metabolism gene clusters revealed an expansion in number and diversity of B. cinerea-specific secondary metabolites relative to S. sclerotiorum. The potential diversity in secondary metabolism might be involved in adaptation to specific ecological niches. Comparative genome analysis revealed the basis of differing sexual mating compatibility systems between S. sclerotiorum and B. cinerea. The organization of the mating-type loci differs, and their structures provide evidence for the evolution of heterothallism from homothallism. These data shed light on the evolutionary and mechanistic bases of the genetically complex traits of necrotrophic pathogenicity and sexual mating. This resource should facilitate the functional studies designed to better understand what makes these fungi such successful and persistent pathogens of agronomic crops. 相似文献