全文获取类型
收费全文 | 1949篇 |
免费 | 175篇 |
出版年
2021年 | 15篇 |
2018年 | 19篇 |
2017年 | 16篇 |
2016年 | 19篇 |
2015年 | 48篇 |
2014年 | 47篇 |
2013年 | 64篇 |
2012年 | 67篇 |
2011年 | 69篇 |
2010年 | 60篇 |
2009年 | 53篇 |
2008年 | 72篇 |
2007年 | 78篇 |
2006年 | 66篇 |
2005年 | 67篇 |
2004年 | 75篇 |
2003年 | 65篇 |
2002年 | 70篇 |
2001年 | 19篇 |
1998年 | 21篇 |
1997年 | 21篇 |
1995年 | 18篇 |
1993年 | 16篇 |
1991年 | 18篇 |
1988年 | 15篇 |
1987年 | 15篇 |
1986年 | 17篇 |
1984年 | 26篇 |
1983年 | 17篇 |
1982年 | 22篇 |
1981年 | 24篇 |
1980年 | 16篇 |
1979年 | 15篇 |
1978年 | 21篇 |
1977年 | 26篇 |
1976年 | 15篇 |
1975年 | 21篇 |
1974年 | 31篇 |
1973年 | 27篇 |
1972年 | 17篇 |
1971年 | 14篇 |
1970年 | 17篇 |
1969年 | 18篇 |
1965年 | 15篇 |
1960年 | 13篇 |
1956年 | 16篇 |
1931年 | 13篇 |
1910年 | 15篇 |
1892年 | 16篇 |
1889年 | 17篇 |
排序方式: 共有2124条查询结果,搜索用时 125 毫秒
181.
The GAGA protein of Drosophila is phosphorylated by CK2 总被引:1,自引:0,他引:1
Bonet C Fernández I Aran X Bernués J Giralt E Azorín F 《Journal of molecular biology》2005,351(3):562-572
The GAGA factor of Drosophila is a sequence-specific DNA-binding protein that contributes to multiple processes from the regulation of gene expression to the structural organisation of heterochromatin and chromatin remodelling. GAGA is known to interact with various other proteins (tramtrack, pipsqueak, batman and dSAP18) and protein complexes (PRC1, NURF and FACT). GAGA functions are likely regulated at the level of post-translational modifications. Little is known, however, about its actual pattern of modification. It was proposed that GAGA can be O-glycosylated. Here, we report that GAGA519 isoform is a phosphoprotein that is phosphorylated by CK2 at the region of the DNA-binding domain. Our results indicate that phosphorylation occurs at S388 and, to a lesser extent, at S378. These two residues are located in a region of the DNA-binding domain that makes no direct contact with DNA, being dispensable for sequence-specific recognition. Phosphorylation at these sites does not abolish DNA binding but reduces the affinity of the interaction. These results are discussed in the context of the various functions and interactions that GAGA supports. 相似文献
182.
183.
Rollo EE Hempson SJ Bansal A Tsao E Habib I Rittling SR Denhardt DT Mackow ER Shaw RD 《Journal of virology》2005,79(6):3509-3516
184.
Databases and information integration for the Medicago truncatula genome and transcriptome
下载免费PDF全文
![点击此处可从《Plant physiology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
185.
Apert syndrome, first described in 1906, is one of the most severe of the craniosynostosis syndromes and is further characterized by midface hypoplasia, syndactyly, and other visceral abnormalities. Affected individuals generally require lifelong management by a multidisciplinary team of health care specialists. Apert syndrome results almost exclusively from one or the other of two point mutations in fibroblast growth factor receptor 2. Tremendous scientific advances have been made recently in understanding the molecular basis for Apert syndrome through clinical genetic, biochemical, and structural approaches. In this review, the authors provide the clinician with a basic overview of these findings and their therapeutic implications. 相似文献
186.
187.
Alavanja MC Bonner MR Furlong CE Allen R Hodgson E 《Journal of biochemical and molecular toxicology》2005,19(3):192-193
© 2005 Wiley Periodicals, Inc. J Biochem Mol Toxicol 19:192–193, 2003; Published online in Wiley InterScience ( www.interscience.wiley.com ). DOI 10.1002/jbt.20083 相似文献
188.
189.
Romagnoli R Baraldi PG Jung MK Iaconinoto MA Carrion MD Remusat V Preti D Tabrizi MA Francesca F De Clercq E Balzarini J Hamel E 《Bioorganic & medicinal chemistry letters》2005,15(18):4048-4052
A new series of compounds, in which the 2-amino-4-methoxyphenyl ring of phenstatin analogue 5 was replaced with 2- or 3-amino-benzoheterocycles, was synthesized and evaluated for antiproliferative activity and inhibition of colchicine binding. The lack of activity of 3',4'-dimethoxy- and 4'-methoxy-benzoyl derivatives (8 and 9, respectively) indicates that the 3',4',5'-trimethoxybenzoyl moiety is critical for the activity. Two compounds, 7 and 11, displayed potent antiproliferative activity, with IC50 values ranging from 25 to 100 nM against a variety of cancer cell lines. Derivative 11 was more active than CA-4 as an inhibitor of tubulin polymerization. The results demonstrated that the antiproliferative activity was correlated with inhibition of tubulin polymerization. 相似文献
190.
Pajon A Ionides J Diprose J Fillon J Fogh R Ashton AW Berman H Boucher W Cygler M Deleury E Esnouf R Janin J Kim R Krimm I Lawson CL Oeuillet E Poupon A Raymond S Stevens T van Tilbeurgh H Westbrook J Wood P Ulrich E Vranken W Xueli L Laue E Stuart DI Henrick K 《Proteins》2005,58(2):278-284
Data management has emerged as one of the central issues in the high-throughput processes of taking a protein target sequence through to a protein sample. To simplify this task, and following extensive consultation with the international structural genomics community, we describe here a model of the data related to protein production. The model is suitable for both large and small facilities for use in tracking samples, experiments, and results through the many procedures involved. The model is described in Unified Modeling Language (UML). In addition, we present relational database schemas derived from the UML. These relational schemas are already in use in a number of data management projects. 相似文献