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41.
Infectious salmon anemia (ISA) is a World Organization for Animal Health (OIE)-listed disease of farmed Atlantic salmon, characterized by slowly developing anemia and circulatory disturbances. The disease is caused by ISA virus (ISAV) in the Orthomyxoviridae family; hence, it is related to influenza. Here we explore the pathogenesis of ISA by focusing on virus tropism, receptor tissue distribution, and pathological changes in experimentally and naturally infected Atlantic salmon. Using immunohistochemistry on ISAV-infected Atlantic salmon tissues with antibody to viral nucleoprotein, endotheliotropism was demonstrated. Endothelial cells lining the circulatory system were found to be infected, seemingly noncytolytic, and without vasculitis. No virus could be found in necrotic parenchymal cells. From endothelium, the virus budded apically and adsorbed to red blood cells (RBCs). No infection or replication within RBCs was detected, but hemophagocytosis was observed, possibly contributing to the severe anemia in fish with this disease. Similarly to what has been done in studies of influenza, we examined the pattern of virus attachment by using ISAV as a probe. Here we detected the preferred receptor of ISAV, 4-O-acetylated sialic acid (Neu4,5Ac(2)). To our knowledge, this is the first report demonstrating the in situ distribution of this sialic acid derivate. The pattern of virus attachment mirrored closely the distribution of infection, showing that the virus receptor is important for cell tropism, as well as for adsorption to RBCs. 相似文献
42.
Kari Bj?rneraas Ivar Herfindal Erling Johan Solberg Bernt-Erik S?ther Bram van Moorter Christer Moe Rolandsen 《Oecologia》2012,168(1):231-243
Identifying factors shaping variation in resource selection is central for our understanding of the behaviour and distribution
of animals. We examined summer habitat selection and space use by 108 Global Positioning System (GPS)-collared moose in Norway
in relation to sex, reproductive status, habitat quality, and availability. Moose selected habitat types based on a combination
of forage quality and availability of suitable habitat types. Selection of protective cover was strongest for reproducing
females, likely reflecting the need to protect young. Males showed strong selection for habitat types with high quality forage,
possibly due to higher energy requirements. Selection for preferred habitat types providing food and cover was a positive
function of their availability within home ranges (i.e. not proportional use) indicating functional response in habitat selection.
This relationship was not found for unproductive habitat types. Moreover, home ranges with high cover of unproductive habitat
types were larger, and smaller home ranges contained higher proportions of the most preferred habitat type. The distribution
of moose within the study area was partly related to the distribution of different habitat types. Our study shows how distribution
and availability of habitat types providing cover and high-quality food shape ungulate habitat selection and space use. 相似文献
43.
The large plasmid pXO1 encoding the anthrax toxin is important for the virulence of Bacillus anthracis. It is essential to cure pXO1 from B. anthracis to evaluate its role in the pathogenesis of anthrax infection. Because conventional methods for curing plasmids (e.g., curing agents or growth at elevated temperatures) can induce mutations in the host chromosomal DNA, we developed a specific and reliable method to eliminate pXO1 from B. anthracis using plasmid incompatibility. Three putative replication origins of pXO1 were inserted into a temperature-sensitive plasmid to generate three incompatible plasmids. One of the three plasmids successfully eliminated the large plasmid pXO1 from B. anthracis vaccine strain A16R and wild type strain A16. These findings provided additional information about the replication/partitioning of pXO1 and demonstrated that introducing a small incompatible plasmid can generate plasmid-cured strains of B. anthracis without inducing spontaneous mutations in the host chromosome. 相似文献
44.
Leif Egil Loe Christophe Bonenfant Erling L. Meisingset Atle Mysterud 《European Journal of Wildlife Research》2012,58(1):195-203
Species distribution models (SDMs) are popular in conservation and management of a wide array of taxa. Often parameterized
with coarse GIS-based environmental maps, they perform well in macro-ecological settings but it is debated if the models can
predict distribution within broadly suitable “known” habitats of interest to local managers. We parameterized SDMs with GIS-derived
environmental variables and location data from 82 GPS-collared female red deer (Cervus elaphus) from two study areas in Norway. Candidate GLM models were fitted to address the effect of spatial scale (landscape vs. home
range), sample size, and transferability between study areas, with respect to predictability (AUC) and explained variance
(Generalized R
2 and deviance). The landscape level SDM captured variation in deer distribution well and performed best on all diagnostic
measures of model quality, caused mainly by a trivial effect of avoidance of non-habitat (barren mountains). The home range
level SDMs were far less predictable and explained comparatively little variation in space use. Landscape scale models stabilized
at the low sample size of 5–10 individuals and were highly transferrable between study areas implying a low degree of individual
variation in habitat selection at this scale. It is important to have realistic expectations of SDMs derived from digital
elevation models and coarse habitat maps. They do perform well in highlighting potential habitat on a landscape scale, but
often miss nuances necessary to predict more fine-scaled distribution of wildlife populations. Currently, there seems to be
a trade-off between model quality and usefulness in local management. 相似文献
45.
SHI Zhaoxing* WANG Hengliang* HU Kun FENG Erling YAO Xiao HUANG Liuyu SU Guofu HUANG Peitang & HUANG Cuifen . Beijing Institute of Biotechnology Beijing China . College of Life Science Shandong Normal University Jinan China . College of Environmental Chemical Engineering Xi’an Jiaotong University Xi’an China Correspondence should be addressed to Huang Liuyu 《中国科学:生命科学英文版》2004,47(6):494-502
~~Screening and identification of Shigella flexneri 2a virulence-related genes induced after invasion of epithelial cells1. Jin, Q., Yuan, Z., Xu, J., Wang, Y., Shen, Y., Lu, W., Wang, J., Liu, H., Yang, J., Yang, P., Zhang, X., Zhang, J., Yang, G, Wu, H., Qu, D., Dong, J., Sun, L., Xue, Y, Zhao, A., Gao, Y., Zhu, J., Kan, B., Ding, K.. Chen, S., Cheng, H., Yao, Z., He, B., Chen, R., Ma, D., Qiang, B., Wen, Y, Hou, Y., Yu, J., Genome sequence of Shigella flexneri 2… 相似文献
46.
47.
The deoxyribonucleic acid (DNA) relationships of 80 strains identified either as Vibrio parahaemolyticus, V. alginolyticus, and V. anguillarum, or as allied marine vibrios were delineated by DNA-DNA competition experiments as well as by measuring the thermal stabilities of the DNA-DNA duplexes formed in direct binding studies. The tested strains included isolates from Japan, Europe, and the United States. The V. parahaemolyticus and V. alginolyticus groups showed an average of 67% homology to one another and 30% to strains of V. anguillarum. Significantly, a number of the isolates from the Pacific Northwest which had been previously identified as V. parahaemolyticus based on morphological, biochemical, and serological evidence were shown either to be strains of V. anguillarum or to belong to as yet unnamed groups. Most strains isolated from diseased salmon in the Pacific Northwest proved to be virtually identical with V. anguillarum type C by DNA homology experiments, thereby differentiating them from similar strains isolated from diseased herring and occasionally from salmon. The latter Pacific Northwest isolates fell into two distinct genotypic groups. A plot of the per cent homology by competition versus the difference in the thermal stabilities of heterologous and homologous duplexes (DeltaT(m,e)) between the same DNA species shows a linear decline in homology of 4.25% per degree of DeltaT(m,e). The use of this relationship for estimating the percentage of the mispaired bases distinguishing DNA preparations directly from competition experiments is discussed. 相似文献
48.
Comparative Studies on the Soluble Components of Adenovirus Types 9 and 15 and the Intermediate Strain 9-15 总被引:5,自引:5,他引:0
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Erling Norby 《Journal of virology》1968,2(10):1200-1210
Five different soluble components of adenovirus types 9, 9-15, and 15 have been identified. These are: (i) a slowly sedimenting, trypsin-resistant, incomplete hemagglutinin (HA). (This component was demonstrable by hemagglutination-enhancement (HE) tests in the presence of heterotypic antisera against members of Rosen's subgroups II and III, but not of subgroup I); (ii) a slowly sedimenting, trypsin-resistant, complete HA, causing only a partial agglutination of cells; (iii) a rapidly sedimenting, incomplete HA, demonstrable by HE tests in the presence of heterotypic antisera against members of all Rosen's subgroups. (Trypsin treatment of this component caused a conversion into slowly sedimenting incomplete HA); (iv) a group-specific complement-fixing (CF) antigen devoid of HA activity; and (v) a rapidly sedimenting, trypsin-sensitive, complete HA, which in the electron microscope was found to represent a dodecahedral aggregate of 12 pentons (a dodecon). On the basis of their biological and physicochemical characteristics, the first four components were interpreted to represent (i) fibers, (ii) a polymer of a few, probably two, fibers, (iii) pentons, and (iv) hexons, respectively. The length of fibers extending from dodecons and virions was estimated to be 11 to 14 nm. A similar value was suggested from exclusion chromatography experiments. Adenovirus types 9 and 15 fibers were recovered in a position intermediate to that of fibers of types 3 and 4, the lengths of which are 10 and 17 nm, respectively. The sequence of elution of different components of types 9 and 9-15 from an anion exchanger was fibers, fiber-aggregate, pentons, hexons, and dodecons. Type 15 components appeared in the same order except for the fact that dodecons eluted before hexons. The molarities of NaCl required to elute the different types 9 and 9-15 components, excluding hexons, were identical. They were distinctly different from those of the corresponding type 15 components. However, hexons of all three serotypes eluted in proximity to each other and there was a slight tendency for type 9-15 hexons to take a position intermediate to those of types 9 and 15. 相似文献
49.
A method is described by which the tissue exposed on sectioning a specimen embedded in paraffin can be visualized in situ. The fixed specimen is impregnated with lead acetate, dehydrated in dioxane, infiltrated with paraffin and embedded. Tissues exposed on sectioning are developed by applying to the cut surface of the block a solution of potassium sulphide in water. Concentrations of the reagents used and the time intervals for the procedure are dependent upon the size of the specimen and upon the degree of contrast required. The method is described as it was applied to the study of a small human fetus in cross section. Representative photographs are included to show the results obtained. 相似文献
50.
Benjamin Cretois Emily G. Simmonds John D. C. Linnell Bram van Moorter Christer M. Rolandsen Erling J. Solberg Olav Strand Vegard Gundersen Ole Roer Jan Ketil Rd 《Ecology and evolution》2021,11(21):15191
Many publications make use of opportunistic data, such as citizen science observation data, to infer large‐scale properties of species’ distributions. However, the few publications that use opportunistic citizen science data to study animal ecology at a habitat level do so without accounting for spatial biases in opportunistic records or using methods that are difficult to generalize. In this study, we explore the biases that exist in opportunistic observations and suggest an approach to correct for them. We first examined the extent of the biases in opportunistic citizen science observations of three wild ungulate species in Norway by comparing them to data from GPS telemetry. We then quantified the extent of the biases by specifying a model of the biases. From the bias model, we sampled available locations within the species’ home range. Along with opportunistic observations, we used the corrected availability locations to estimate a resource selection function (RSF). We tested this method with simulations and empirical datasets for the three species. We compared the results of our correction method to RSFs obtained using opportunistic observations without correction and to RSFs using GPS‐telemetry data. Finally, we compared habitat suitability maps obtained using each of these models. Opportunistic observations are more affected by human access and visibility than locations derived from GPS telemetry. This has consequences for drawing inferences about species’ ecology. Models naïvely using opportunistic observations in habitat‐use studies can result in spurious inferences. However, sampling availability locations based on the spatial biases in opportunistic data improves the estimation of the species’ RSFs and predicted habitat suitability maps in some cases. This study highlights the challenges and opportunities of using opportunistic observations in habitat‐use studies. While our method is not foolproof it is a first step toward unlocking the potential of opportunistic citizen science data for habitat‐use studies. 相似文献