全文获取类型
收费全文 | 101篇 |
免费 | 10篇 |
出版年
2022年 | 1篇 |
2021年 | 1篇 |
2017年 | 1篇 |
2016年 | 2篇 |
2015年 | 2篇 |
2014年 | 2篇 |
2013年 | 1篇 |
2012年 | 6篇 |
2011年 | 1篇 |
2010年 | 2篇 |
2009年 | 3篇 |
2007年 | 1篇 |
2006年 | 4篇 |
2005年 | 2篇 |
2004年 | 5篇 |
2003年 | 1篇 |
2002年 | 5篇 |
2001年 | 4篇 |
2000年 | 3篇 |
1998年 | 7篇 |
1997年 | 1篇 |
1996年 | 1篇 |
1995年 | 2篇 |
1993年 | 1篇 |
1992年 | 2篇 |
1991年 | 1篇 |
1990年 | 7篇 |
1989年 | 3篇 |
1988年 | 5篇 |
1987年 | 7篇 |
1986年 | 2篇 |
1985年 | 2篇 |
1982年 | 1篇 |
1980年 | 2篇 |
1979年 | 6篇 |
1977年 | 1篇 |
1976年 | 5篇 |
1975年 | 3篇 |
1974年 | 4篇 |
1972年 | 1篇 |
排序方式: 共有111条查询结果,搜索用时 187 毫秒
81.
Despite the recent introduction of biological response modifiers and potent new small-molecule antirheumatic drugs, the efficacy of methotrexate is nearly unsurpassed in the treatment of inflammatory arthritis. Although methotrexate was first introduced as an antiproliferative agent that inhibits the synthesis of purines and pyrimidines for the therapy of malignancies, it is now clear that many of the anti-inflammatory effects of methotrexate are mediated by adenosine. This nucleoside, acting at one or more of its receptors, is a potent endogenous anti-inflammatory mediator. In confirmation of this mechanism of action, recent studies in both animals and patients suggest that adenosine-receptor antagonists, among which is caffeine, reverse or prevent the anti-inflammatory effects of methotrexate. 相似文献
82.
Corn samples were collected in 1999 from three departments of Entre Réos province, Argentina, and were surveyed for mould
contamination and natural occurrence ofFusarium mycotoxins, ochratoxin A and aflatoxins.Fusarium verticillioides was the most prevalent fungal species recorded at all departments. Zearalenone, deoxynivalenol and ochratoxin A were not
found in any samples. Only one of the 52 corn samples analysed was contaminated with aflatoxin B1 (17 μg/kg). Fumonisin B1 was found in 58 % of samples (range of positive samples: 47– 3,347 μg/kg), fumonisin B2 in 33.0 % (range of positive samples: 23–537 μg/kg) and fumonisin B3 in 25.0 % (range of positive samples: 24–287 μg/kg) of them. This is the first report on the natural occurrence of mycotoxins
in corn from Entre Ríos province, Argentina. Levels of fumonisins were lower than detected in other Argentinian provinces. 相似文献
83.
The rate coefficient K2 of the physical exchange of oxygen between flowing water and the atmosphere is dependent on water temperature and the hydraulic variables: mean velocity of flow U, mean depth H, and slope S of the stream channel. 36 equations from the literature predicting K2 on basis of combinations of U, H and S have been tested against 144 corresponding measurements of K2 and hydraulic variables taken in 10 reaches in 6 Danish streams with different development of the submerged macrophyte community. A statistical analysis showed that all equations except one had to be rejected. To improve predictive accuracy an equation K2 (20 °C) = 8784 U0.734H−0.420 S0.930 was developed applying multiple linear regression. This equation allows K2-predictions for streams where the hydraulic variables U range from 0.06 to 0.52 m s−1, H from 0.12 to 1.37 m and S from 0.3 to 7.4 × 10−3 m m−1, respectively. Using this equation the lower and upper 95 % limit of confidence for a given K2-prediction (K2p) are K2p/1.95 and K2p × 1.95, indicating that such equations at best approximate the real K2. Therefore K2 should preferably be measured. 相似文献
84.
85.
Shortening of surface sediment cores during sampling 总被引:1,自引:0,他引:1
Coring in recent lake sediments is most easily performed using lightweight open barrel gravity corers. The cores will in most cases have the fine lamination intact and they may thus be interpreted as representative of the sediment in situ.Parallel cores taken by means of other techniques have shown that cores taken with open barrel gravity corers become shorter than the distance of penetration in the sediment. This investigation shows that the shortening depends both on the corer diameter and on its velocity during the penetration. Experiments also show that soft layers become more reduced in thickness than stiffer ones. This may lead to misinterpretation of sedimentation rate during special periods. Seasonally occurring sediment fractions may be diluted or concentrated during the coring. 相似文献
86.
Zhu F Erlandsen H Ding L Li J Huang Y Zhou M Liang X Ma J Wu H 《The Journal of biological chemistry》2011,286(30):27048-27057
Serine-rich repeat glycoproteins (SRRPs) are a growing family of bacterial adhesins found in many streptococci and staphylococci; they play important roles in bacterial biofilm formation and pathogenesis. Glycosylation of this family of adhesins is essential for their biogenesis. A glucosyltransferase (Gtf3) catalyzes the second step of glycosylation of a SRRP (Fap1) from an oral streptococcus, Streptococcus parasanguinis. Although Gtf3 homologs are highly conserved in SRRP-containing streptococci, they share minimal homology with functionally known glycosyltransferases. We report here the 2.3 ? crystal structure of Gtf3. The structural analysis indicates that Gtf3 forms a tetramer and shares significant structural homology with glycosyltransferases from GT4, GT5, and GT20 subfamilies. Combining crystal structural analysis with site-directed mutagenesis and in vitro glycosyltransferase assays, we identified residues that are required for UDP- or UDP-glucose binding and for oligomerization of Gtf3 and determined their contribution to the enzymatic activity of Gtf3. Further in vivo studies revealed that the critical amino acid residues identified by the structural analysis are crucial for Fap1 glycosylation in S. parasanguinis in vivo. Moreover, Gtf3 homologs from other streptococci were able to rescue the gtf3 knock-out mutant of S. parasanguinis in vivo and catalyze the sugar transfer to the modified SRRP substrate in vitro, demonstrating the importance and conservation of the Gtf3 homologs in glycosylation of SRRPs. As the Gtf3 homologs only exist in SRRP-containing streptococci, we conclude that the Gtf3 homologs represent a unique subfamily of glycosyltransferases. 相似文献
87.
88.
S L Erlandsen A Greet Bittermann J White A Leith M Marko 《The journal of histochemistry and cytochemistry》2001,49(7):809-819
The aim of this study was to develop a model for the detection of individual cell adhesion molecules (CAMs) in the glycocalyx of spread human platelets using high-resolution cryo-field emission scanning electron microscopy (cryoFESEM). Three surface glycoprotein CAMs, P-selectin (CD62P), GPIba in the GPI-IX complex (CD42a/CD42b alpha,b beta), and the integrin GPIIbIIIa (CD41/CD61) in the human platelet were selected on the basis of their unique topographic shape. Spread human platelets were indirectly immunolabeled with 10-nm colloidal gold and then cryoimmobilized. After sublimation of water from the cryoimmobilized sample, partially freeze-dried platelets were coated unidirectionally with Pt, stabilized with carbon, and examined in an in-lens cryoFESEM using high-resolution backscattered electron imaging. CAMs were detected by indirect immunogold labeling and the length of each type of CAM was determined using analysis of differences in parallax as measured in the software program Sterecon. Our results demonstrate the efficacy of using high-resolution cryoFESEM to recognize and detect individual CAMs in the glycocalyx. Further advances in production of metal coatings with finer granularity, together with improvements in imaging (tilting and angle of stereo images), may provide better definition of the topography associated with glycosylation and formation of multimeric CAM complexes. (J Histochem Cytochem 49:809-819, 2001) 相似文献
89.
90.
S S Spicer S L Erlandsen A C Wilson M F Hammer R A Hennigar B A Schulte 《The journal of histochemistry and cytochemistry》1987,35(11):1231-1244
A wide range of tissues from three interfertile species of mice and an interspecific hybrid was examined with lectins conjugated to peroxidase to localize specifically glycoconjugates containing terminal alpha-N-acetylgalactosamine, alpha-galactose, and alpha-fucose, and the terminal disaccharide galactose-(beta 1----3)-N-acetylgalactosamine. This battery of lectins disclosed marked heterogeneity of glycoconjugates in different histological sites in a given animal and even between cells in a presumably homogeneous cell population within an organ. No variation with any lectin was observed between individuals of two closely related inbred strains of Mus domesticus at any specific histological or cytological site. In contrast, littermates of an outbred strain of Mus castaneus differed in binding of certain lectins at various sites, attesting to a genetic basis for individual variation. Hybrids between castaneus and domesticus mice also showed individual variation. Moreover, extensive differences between the mouse species were demonstrable with every lectin in glycoconjugates of stored secretions, Golgi cisternae, and apical or basolateral plasmalemma in many cell types. Totaling the differences in tabulated staining intensities for each possible species pair gave a measure of the overall extent of difference at 53 histological sites. According to this measure, the three species are about equally divergent from one another. Some differences between species appeared to depend on histological rather than histochemical variation, as, for example, a greater abundance of granular duct cells in the sublingual and submandibular glands in Mus hortulanus. Other differences were apparently derived from pathological change, as exemplified by casts and lymphoid infiltrates in kidney and structurally atypical submandibular gland lobules in Mus castaneus, and possibly by infiltrating cells in intestinal lamina propria and epithelium in Mus castaneus and hortulanus. 相似文献