全文获取类型
收费全文 | 5774篇 |
免费 | 539篇 |
国内免费 | 2篇 |
专业分类
6315篇 |
出版年
2022年 | 47篇 |
2021年 | 81篇 |
2020年 | 47篇 |
2019年 | 55篇 |
2018年 | 57篇 |
2017年 | 77篇 |
2016年 | 105篇 |
2015年 | 193篇 |
2014年 | 202篇 |
2013年 | 257篇 |
2012年 | 323篇 |
2011年 | 313篇 |
2010年 | 224篇 |
2009年 | 185篇 |
2008年 | 233篇 |
2007年 | 271篇 |
2006年 | 240篇 |
2005年 | 243篇 |
2004年 | 223篇 |
2003年 | 205篇 |
2002年 | 208篇 |
2001年 | 156篇 |
2000年 | 155篇 |
1999年 | 111篇 |
1998年 | 87篇 |
1997年 | 75篇 |
1996年 | 50篇 |
1995年 | 58篇 |
1994年 | 42篇 |
1993年 | 44篇 |
1992年 | 84篇 |
1991年 | 48篇 |
1990年 | 85篇 |
1989年 | 83篇 |
1988年 | 71篇 |
1987年 | 54篇 |
1986年 | 62篇 |
1985年 | 70篇 |
1984年 | 56篇 |
1983年 | 50篇 |
1982年 | 42篇 |
1980年 | 46篇 |
1979年 | 66篇 |
1978年 | 58篇 |
1977年 | 58篇 |
1976年 | 43篇 |
1974年 | 57篇 |
1973年 | 45篇 |
1972年 | 50篇 |
1970年 | 47篇 |
排序方式: 共有6315条查询结果,搜索用时 15 毫秒
11.
12.
13.
F. Mark U. Becker J. N. Herak D. Schulte-Frohlinde 《Radiation and environmental biophysics》1989,28(2):81-99
Summary Two expressions are given for the survival dose of DNA exposed to high-energy radiation in aqueous solution in the presence of a scavenger. They are derived from a model where a diffusion controlled reaction of OH radicals occurs on the surface of the DNA macromolecules in competition with scavenging in the bulk of the solution. The DNA molecules are approximated either by spheres or by cylinders. The model based on molecules of spherical shape corresponds closely to that developed by van Rijn et al. [20]. Expressions obtained from the cylindrical model are used to account for the dependence on the scavenger concentration of some experimentally measured quantities, namely the survival dose and theG value for single-strand breaks upon Co -irradiation ofX 174 DNA and polyadenylic acid, respectively.In memoriam Prof. Dr. O.E. Polansky 相似文献
14.
In many cases it can be demonstrated that the amount of plant protectives and plant treatments (pesticides) in drinking-water exceeds the permitted levels of the drinking-water decree which will be effective on October 1st, 1989. These components are in parts toxicologically important. Therefore, an examination was made on how far pesticides are removed during the conventional purification of dialysis water, but especially during the reverse osmosis. Retention rates of a reverse osmosis plant for 14 different pesticides were discovered which were used in different concentrations and compositions. In part 2 of this contribution the results of the investigation are presented. The figures demonstrate that almost all of the examined components were retained with an effectiveness of 92-98%. The elimination efficiency did not depend on the basic concentration of the pesticides. After an initial phase of 50 h duration, the permeat concentration reached a constant value which did not alter even after more than 700 h. 相似文献
15.
M A Simmons E C Johnson J B Becker D G Todd V E Reichenbecher W D McCumbee G L Wright 《FEBS letters》1989,254(1-2):137-140
The effects of an immunoaffinity-purified putative endogenous hypertensive factor (HF) on voltage-dependent calcium current in frog cardiac myocytes were assessed. In 9 out of 10 cells, HF reversibly increased the peak amplitude of the calcium current. HF increased peak calcium current density at -5 mV from a control level of 1.8 +/- 1.3 pA/pF (mean +/- SD) to 4.4 +/- 2.0 pA/pF. HF shifted the peak of the calcium current-voltage relationship in the hyperpolarizing direction. HF shifted the voltage dependence of the inactivation of the calcium current to more negative potentials with prepulses from -80 to 0 mV, but the inactivation was not affected with prepulses more positive than 0 mV. Modulation of the voltage-dependent calcium current by HF may be the mechanism underlying its pressor effects. 相似文献
16.
Binary vectors which allow the exchange of plant selectable markers and reporter genes. 总被引:26,自引:1,他引:25 下载免费PDF全文
D Becker 《Nucleic acids research》1990,18(1):203
17.
18.
Caudoxirene, the spermatozoid-releasing and attracting factor in the marine brown alga Perithalia caudata (Phaeophyceae, Sporochnales) 总被引:2,自引:0,他引:2
Caudoxirene (cis-3-(1,2-trans-epoxy-but-3-enyl)-4-vinyl-cyclopentene) is a new gamete releasing factor from Perithalia caudata (Sporochnales). Its threshold concentration is found at 30 pmol for gamete release. Multifidene, viridiene and a Z-isomer of caudoxirene were identified as by-products or trace constituents. 相似文献
19.
High yield enzymatic conversion of intravascular leukotriene A4 in blood-free perfused lungs 总被引:4,自引:0,他引:4
Experiments to investigate the fate of intravascularly administered leukotriene (LT) A4, an unstable intermediate of LT generation, were performed in isolated, ventilated, and blood-free perfused rabbit lungs. LT extracted from the lung effluent were separated by different reverse phase and straight phase HPLC procedures as methylated and nonmethylated compounds. Identity of eluting LT was confirmed by UV spectrum analysis and immunoreactivity. Pulmonary artery injection of 75 to 300 nmol of LTA4 resulted in the rapid appearance of cysteinyl-LT as well as LTB4 in the recirculating perfusate. The yield of these enzymatically generated LTA4 metabolites vs non-enzymatic hydrolysis products (6-trans-LTB4, 5-trans-epi-LTB4, 5,6-dihydroxyeicosatetraenoic acids) ranged above 90%. Experiments with application of tritiated LTA4 showed exclusive origin of the detected LT from the exogenously applied precursor. The time course of cysteinyl-LT appearance in the perfusate suggested metabolism of LTC4 via LTD4 to LTE4, whereas there was no evidence for LTB4 omega-oxidation. In the dose range of LTA4 used, the enzymatic conversion of this LT precursor did not approach saturation. Collectively, these data indicate that the intact pulmonary vasculature contains a hitherto not described capacity for enzymatic conversion of intravascularly offered LTA4 to both cysteinyl-LT and LTB4. This may be of biological significance for a putative transcellular biosynthesis of LT in the pulmonary microcirculation upon contact with LTA4 feeder cells, such as activated granulocytes. 相似文献
20.
B Carragher D A Bluemke M Becker W A McDade M J Potel R Josephs 《Journal of molecular biology》1988,199(2):383-388
We have examined the structure of hemoglobin S fibers, which are associated into large bundles, or fascicles. Electron micrographs of embedded and cross-sectioned fascicles provide an end-on view of the component fibers. The cross-sectional images are rotationally blurred as a result of the twist of the fiber within the finite thickness of the section. We have applied restoration techniques to recover a deblurred image of the fiber. The first step in this procedure involved correlation averaging images of cross-sections of individual fibers in order to improve the signal-to-noise ratio. The rotationally blurred image was then geometrically transformed to polar co-ordinates. In this space, the rotational blur is transformed into a linear blur. The linearly blurred image is the convolution of the unblurred image and a point spread function that can be closely approximated by a square pulse. Deconvolution in Fourier space, followed by remapping to Cartesian co-ordinates, produced a deblurred image of the original micrograph. The deblurred images indicate that the fiber is comprised of 14 strands of hemoglobin S. This result provides confirmation of the fiber structure determined using helical reconstruction techniques and indicates that the association of fibers into ordered arrays does not alter their molecular structure. 相似文献