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991.
In many bacteria, the twin arginine translocation (Tat) system transports folded proteins across the cytoplasmic membrane, and these proteins can play a role in symbiotic or pathogenic infections. A role for the Vibrio fischeri Tat system was identified during symbiotic colonization of its host Euprymna scolopes, demonstrating a function for the Tat system in host colonization by a member of the Vibrionaceae. Using bioinformatics, mutant analyses, and green fluorescent protein fusions, a set of Tat-targeted proteins in V. fischeri was identified. 相似文献
992.
Hoffmann M Zhao S Luo Y Li C Folster JP Whichard J Allard MW Brown EW McDermott PF 《Journal of bacteriology》2012,194(12):3274-3275
Salmonella enterica serovar Heidelberg has caused numerous outbreaks in humans. Here, we report draft genomes of five isolates of serovar Heidelberg associated with the recent (2011) multistate outbreak linked to ground turkey in the United States. Isolates 2011K-1110 and 2011K-1132 were recovered from humans, while isolates 2011K-1138, 2011K-1224, and 2011K-1225 were recovered from ground turkey. Whole-genome sequence analysis of these isolates provides a tool for studying the short-term evolution of these epidemic clones. 相似文献
993.
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995.
Francisella tularensis is a highly virulent bacterium responsible for the zoonotic disease tularemia. It is a facultative intracellular pathogen that replicates in the cytoplasm of host cells, particularly in macrophages. Here we show that F. tularensis live vaccine strain (LVS) expresses a novel small RNA (sRNA), which modulates the virulence capacities of the bacterium. When this sRNA, designated FtrC (for Francisella tularensisRNA C), is expressed at high levels, F. tularensis replicates in macrophages less efficiently than the wild-type parent strain. Similarly, high expression of FtrC reduces the number of viable bacteria recovered from the spleen and liver of infected mice. Our data demonstrate that expression of gene FTL_1293 is regulated by FtrC. Furthermore, we show by in vitro gel shift assays that FtrC interacts specifically with FTL_1293 mRNA and that this happens independently of the RNA chaperone Hfq. Remarkably, FtrC interacts only with full-length FTL_1293 mRNA. These results, combined with a bioinformatic analysis, indicate that FtrC interacts with the central region of the mRNA and hence does not act by sterically hindering access of the ribosome to the mRNA. We further show that gene FTL_1293 is not required for F. tularensis virulence in vitro or in vivo, which indicates that another unidentified FtrC target modulates the virulence capacity of the bacterium. 相似文献
996.
Interactive responses of grass litter decomposition to warming,nitrogen addition and detritivore access in a temperate old field 总被引:1,自引:0,他引:1
Plant litter decomposition has been studied extensively in the context of both climate warming and increased atmospheric N deposition. However, much of this research is based on microbial responses, despite the potential for detritivores to contribute substantially to litter breakdown. We measured litter mass-loss responses to the combined effects of warming, N addition and detritivore access in a grass-dominated old field. We concurrently assessed the roles of litter treatment origin vs. microenvironment (direct warming and N-addition effects) to elucidate the mechanisms through which these factors affect decomposition. After 6 weeks, mass loss increased in N-addition plots, and it increased with detritivore access in the absence of warming. After 1 year, warming, N addition, and detritivore access all increased litter mass loss, although the effects of N addition and warming were non-additive in the detritivore-access plots. For the litter-origin experiment, mass loss after 6 weeks increased in litter from N-addition plots and warmed plots, but unlike the overall decomposition response, the N-addition effect was enhanced by detritivore access. Conversely, for the microenvironment experiment, detritivore access only increased mass loss in unfertilized plots. After 1 year, detritivore access increased mass loss in the litter-origin and microenvironment experiments, but there were no warming or N-addition effects. Overall, our results provide support for a substantial role of detritivores in promoting litter mass loss in our system. Moreover, they reveal important interactions between litter origin, microclimate and detritivores in determining decomposition responses to global change. 相似文献
997.
Casellas J Gularte RJ Farber CR Varona L Mehrabian M Schadt EE Lusis AJ Attie AD Yandell BS Medrano JF 《Genetics》2012,191(1):247-259
Transmission ratio distortion (TRD) is the departure from the expected genotypic frequencies under Mendelian inheritance. This departure can be due to multiple physiological mechanisms during gametogenesis, fertilization, fetal and embryonic development, and early neonatal life. Although a few TRD loci have been reported in mouse, inheritance patterns have never been evaluated for TRD. In this article, we developed a Bayesian binomial model accounting for additive and dominant deviation TRD mechanisms. Moreover, this model was used to perform genome-wide scans for TRD quantitative trait loci (QTL) on six F2 mouse crosses involving between 296 and 541 mice and between 72 and 1854 genetic markers. Statistical significance of each model was checked at each genetic marker with Bayes factors. Genome scans revealed overdominance TRD QTL located in mouse chromosomes 1, 2, 12, 13, and 14 and additive TRD QTL in mouse chromosomes 2, 3, and 15, although these results did not replicate across mouse crosses. This research contributes new statistical tools for the analysis of specific genetic patterns involved in TRD in F2 populations, our results suggesting a relevant incidence of TRD phenomena in mouse with important implications for both statistical analyses and biological research. 相似文献
998.
Benthic macroinvertebrate fauna plays a major role in river ecosystems, especially those of tropical islands. Since there is no information on the distribution of benthic invertebrates along a Jamaican river, we report here on the composition of the benthic fauna of the Buff Bay river, on the Northern coast of Jamaica. A total of 14 samples were collected from five sites, using kick nets and a Surber sampler, between May 1997 and October 1998. We also examined the applicability of the rhithron/potamon model, and some of the premises of the River Continuum Concept (RCC) in relation to the distribution of invertebrate taxa. The results showed a total of 38 taxa of identified invertebrates. A group of dominant taxa, composed mainly of immature stages of insects, occurred at all sites. Two notable characteristics of the river were the absence of a true potamonic fauna and the low representation of the shredder functional feeding group in the community We conclude that, while there was minor variation in the composition of the benthic macroinvertebrate fauna among the sites, this was a response to local conditions within the river system. The characteristics of the community did not conform to either of the models. 相似文献
999.
Bastin C Feyers D Majerus S Balteau E Degueldre C Luxen A Maquet P Salmon E Collette F 《PloS one》2012,7(1):e29905
The directed forgetting paradigm is frequently used to determine the ability to voluntarily suppress information. However, little is known about brain areas associated with information to forget. The present study used functional magnetic resonance imaging to determine brain activity during the encoding and retrieval phases of an item-method directed forgetting recognition task with neutral verbal material in order to apprehend all processing stages that information to forget and to remember undergoes. We hypothesized that regions supporting few selective processes, namely recollection and familiarity memory processes, working memory, inhibitory and selection processes should be differentially activated during the processing of to-be-remembered and to-be-forgotten items. Successful encoding and retrieval of items to remember engaged the entorhinal cortex, the hippocampus, the anterior medial prefrontal cortex, the left inferior parietal cortex, the posterior cingulate cortex and the precuneus; this set of regions is well known to support deep and associative encoding and retrieval processes in episodic memory. For items to forget, encoding was associated with higher activation in the right middle frontal and posterior parietal cortex, regions known to intervene in attentional control. Items to forget but nevertheless correctly recognized at retrieval yielded activation in the dorsomedial thalamus, associated with familiarity-based memory processes and in the posterior intraparietal sulcus and the anterior cingulate cortex, involved in attentional processes. 相似文献
1000.