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81.
In this study, we applied DNA barcoding to identify species using short DNA sequence analysis. We examined the utility of
DNA barcoding by identifying 53 Korean freshwater fish species, 233 other freshwater fish species, and 1339 saltwater fish
species. We successfully developed a web-based molecular identification system for fish (MISF) using a profile hidden Markov
model. MISF facilitates efficient and reliable species identification, overcoming the limitations of conventional taxonomic
approaches. MISF is freely accessible at . 相似文献
82.
To provide more reliable genetic information on species and minimize experimental errors, biologists increase the number of genetic markers available and then carefully select optimal markers from a large candidate pool. We developed nine novel microsatellite markers from the Korean field mouse (Apodemus peninsulae), which is one of the most dominant forest animals in South Korea. The mean observed and expected heterozygosities across nine markers were 0.65 and 0.73, respectively, with an average polymorphic information content of 0.70. Using 17 microsatellite markers (nine polymorphic markers in this study, in combination with eight previously reported for the species), we conducted genetic analysis on the animals from six sampling locations. These locations are divided into the eastern (EAST) and the western (WEST) sides of the Taebaek mountain ranges in South Korea. Genetic diversity was high at both groups, with the mean expected heterozygosity of 0.77 in EAST and 0.78 in WEST. However, we did not observe strong evidence of genetic divergence between two groups. Future genetic research with more samples incorporating ecological study may clarify population structure in the species and the hypothesis of the mountains discontinuity of gene flow. 相似文献
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84.
Soo Eo Jung Hyun Woo-Shin Lee Tae Choi Shin-Jae Rhim Kazuhiro Eguchi 《Journal of Ethology》2002,20(1):43-47
This study was conducted using random amplified polymorphic DNA analysis to examine whether the effects of topographical
structure on the dispersal of black-billed magpie Pica pica sericea are reflected in its population genetic structure. The black-billed magpie is typically seen in the lowlands and is remarkably
sedentary in Korea and Japan. The unweighted pair-group method of clustering analysis showed two main clusters: five populations
in the western region of the Bekdudegan mountains (WRBM) in one cluster and five populations in the eastern region of the
Bekdudegan mountains (ERBM), including the Japanese population in the other cluster. The populations in WRBM appeared to be
diverged from the populations in ERBM. Analysis of molecular variance revealed that the populations in ERBM were more genetically
divergent from each other than were those in WRBM. The high-rising mountains, very rugged topographical features and the sea
in ERBM may have resulted in a lower dispersal rate and larger genetic variation among populations in ERBM compared to those
in WRBM. These results suggest that the topographical structure may have an influence on the dispersal and population genetic
structure of the black-billed magpie.
Electronic Publication 相似文献
85.
Protein tyrosine kinases (PTKs) play a central role in the modulation of a wide variety of cellular events such as differentiation,
proliferation and metabolism, and their unregulated activation can lead to various diseases including cancer and diabetes.
PTKs represent a diverse family of proteins including both receptor tyrosine kinases (RTKs) and non-receptor tyrosine kinases
(NRTKs). Due to the diversity and important cellular roles of PTKs, accurate classification methods are required to better
understand and differentiate different PTKs. In addition, PTKs have become important targets for drugs, providing a further
need to develop novel methods to accurately classify this set of important biological molecules. Here, we introduce a novel
statistical model for the classification of PTKs that is based on their structural features. The approach allows for both
the recognition of PTKs and the classification of RTKs into their subfamilies. This novel approach had an overall accuracy
of 98.5% for the identification of PTKs, and 99.3% for the classification of RTKs. 相似文献