全文获取类型
收费全文 | 17416篇 |
免费 | 1456篇 |
国内免费 | 4篇 |
专业分类
18876篇 |
出版年
2024年 | 23篇 |
2023年 | 160篇 |
2022年 | 129篇 |
2021年 | 289篇 |
2020年 | 265篇 |
2019年 | 276篇 |
2018年 | 629篇 |
2017年 | 531篇 |
2016年 | 603篇 |
2015年 | 557篇 |
2014年 | 633篇 |
2013年 | 1013篇 |
2012年 | 1376篇 |
2011年 | 1533篇 |
2010年 | 812篇 |
2009年 | 595篇 |
2008年 | 1162篇 |
2007年 | 1071篇 |
2006年 | 1065篇 |
2005年 | 815篇 |
2004年 | 850篇 |
2003年 | 821篇 |
2002年 | 723篇 |
2001年 | 441篇 |
2000年 | 554篇 |
1999年 | 301篇 |
1998年 | 185篇 |
1997年 | 131篇 |
1996年 | 122篇 |
1995年 | 105篇 |
1994年 | 96篇 |
1993年 | 115篇 |
1992年 | 92篇 |
1991年 | 77篇 |
1990年 | 64篇 |
1989年 | 54篇 |
1988年 | 45篇 |
1987年 | 44篇 |
1986年 | 32篇 |
1985年 | 51篇 |
1984年 | 39篇 |
1983年 | 36篇 |
1982年 | 54篇 |
1981年 | 36篇 |
1980年 | 38篇 |
1979年 | 29篇 |
1978年 | 27篇 |
1977年 | 25篇 |
1974年 | 20篇 |
1973年 | 21篇 |
排序方式: 共有10000条查询结果,搜索用时 0 毫秒
31.
32.
A number of expeditions to the area of Salar de Atacama, Chile, 68° 15'W, 20° 30'S, have involved studies of the biological
and chemical features of Lake Tebenquiche, situated in the interior of the salar. Chemically, Tebenquiche is hypersaline,
with practically anoxic waters dominated by sodium and chloride ions but with high concentrations of sulphate also. The lake
is surrounded and invaded by macrophytes, dominated by Scirpus olmeyi and Juncus, which provide organic material for the formation of bacterial mats. The fauna of limnetic crustaceans is almost exclusively
of Artemia salina. The most important genera of bacteria are: Marinomonas, Halobacterium, Acinetobacter and the sulphur reductors Vibrio and Bacillus. The Cyanobacteria are represented exclusively by Oscillatoria. 相似文献
33.
34.
35.
Victor H. Kollman John L. Hanners Robert E. London Enrique G. Adame Thomas E. Walker 《Carbohydrate research》1979,73(1):193-202
The blue-green alga Agmenellum quadruplicatum (strain PR6) has been used to prepare photobiosynthetically 13C-labeled d-glucose, 2-O-(α-d-glucopyranosyl)-glyceric acid (glucosylglycerate), 2-hydroxy-1-(hydroxymethyl)ethyl α-d-gluco-pyranoside (glucosylglycerol), and α-d-glueopyranosyl β-d-fructofuranoside (sucrose). When grown to a cell density of 4.4 g.L-1 (dry weight) under nitrate-nitrogen limiting growth conditions for 120 h, the algal cells contained 38% of the dry-cell weight as(1 → 4)-α-d-glucan (amylose). About 1% of the dry-cell weight was glucosylglycerol, glucosylglycerate, and sucrose. Glutamate was obtained, together with carbohydrates of low molecular weight, when the cells were extracted with chloroform-methanol; d-glucose was recovered from the extracted cells by acid hydrolysis of the starch. The algae were grown by using 20 mol% [13C] carbon dioxide for preparation of labeled carbohydrates and for cellular component identification by whole-cell n.m.r. spectroscopy. 相似文献
36.
JoséLuis Avila Antonio Bretaña María Argelia Casanova Angela Avila Francisco Rodríguez 《Experimental parasitology》1979,48(1):27-35
A liquid medium was developed for the continuous cultivation of Trypanosoma cruzi. Among the several highly purified macromolecules tested only bovine liver catalase, horseradish peroxidase, lactoperoxidase, and bovine hemoglobin supported the continuous growth, at high yield, of mice-virulent Trypanosoma cruzi; other hemoproteins were inactive. Bovine liver catalase showed optimal Trypanosoma cruzi growth-promoting activity, parasites reaching 20 × 106 parasites/ml (95% epimastigotes) at about 10 days in most of the 45 subpassages to date. Furthermore, this protein in the incubation medium provided all the amino acid requirements of actively growing parasites, thus eliminating the need for exogeneous free amino acids. Additional experiments revealed that the hemoprotein's growth-promoting activity was independent of any enzymatic activity and that reconstituting the exact protein composition by means of exogeneous amino acids did not support parasite multiplication, suggesting the importance of the primary structure of the active proteins for growth-promoting activity. These active macromolecules supported the multiplication of five different strains of Trypanosoma cruzi, but did not support Leishmania brasiliensis or Leishmania mexicana proliferation, suggesting species specificity. 相似文献
37.
Enrique J. La Motta 《Biotechnology and bioengineering》1976,18(10):1359-1370
A method for calculating the mass transfer coefficient in a biological film system, under turbulent flow conditions, is presented. It is experimentally found that fluid velocity has a positive effect on the rate of substrate utilization when the system is operated in other than the kinetic regime. A correlation is developed which indicates a dependence of the mass transfer coefficient on the fluid velocity raised to the 0.7 power. 相似文献
38.
A soluble purified form of Micrococcus lysodeikticus ATPase (form BAT, from strain B, active, trypsin-stimulated) was stimulated 100% by trypsin and this stimulation was inhibited by preincubation of the protease with phenyl methyl sulphonylfluoride. This form of the enzyme was also stimulated 125–150% by filtration on Sephadex G-200. Analysis by sodium dodecyl sulphate-gel electrophoresis showed that stimulation of this form of M. lysodeikticus ATPase was always accompanied by the disappearance of a subunit of mol. wt. 25 000 (ε subunit). It suggests that this subunit is the natural inhibitor of M. lysodeikticus ATPase. In the case of ATPase stimulation by trypsin, a partial and limited degradation of the α subunit was also observed. The interaction between the ε subunit and the rest of the ATPase complex was reversibly affected by pH, suggesting its non-covalent nature. 相似文献
39.
—Alcohol:NAD oxidoreductase (EC 1.1.1.1) was studied in brain cortex, hypothalamus, cerebellum and midbrain of adult and immature rats, and in the whole encephalon of neonatal rats. The rats used in this study were (i) from a colony which has been given 12% (v/v) aqueous ethanol as the only fluid for 54 generations (‘E.F.’ rats); (ii) rats removed from this colony after the forty-eighth generation and thereafter fed water instead of the alcohol solution (‘E.F./H2O’ rats); and (iii) normal rats. Enzyme activity in the 20,000 g supernatant of tissue homogenates was measured by the method of Raskin and Sokoloff. Activity was found to be highest in neonatal rat brain and to decrease as the age increased. Activity in the hypothalamus of adult E.F. rats was significantly higher than that found in the same region of adult E.F./H2O rats. Immature rat cerebellum alcohol:NAD oxidoreductase activity was higher both in ‘E.F.’ and ‘E.F./H2O’ suggesting a possible genetic change be involved in this CNS region. It may be concluded that, with the exception of neonatal rats, ethanol consumption induces an increase in rat CNS alcohol :NAD oxidoreductase activity. 相似文献
40.