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91.
92.
Brian M. Shamblin Alan B. Bolten F. Alberto Abreu-Grobois Karen A. Bjorndal Luis Cardona Carlos Carreras Marcel Clusa Catalina Monzón-Argüello Campbell J. Nairn Janne T. Nielsen Ronel Nel Luciano S. Soares Kelly R. Stewart Sibelle T. Vila?a Oguz Türkozan Can Yilmaz Peter H. Dutton 《PloS one》2014,9(1)
Previous genetic studies have demonstrated that natal homing shapes the stock structure of marine turtle nesting populations. However, widespread sharing of common haplotypes based on short segments of the mitochondrial control region often limits resolution of the demographic connectivity of populations. Recent studies employing longer control region sequences to resolve haplotype sharing have focused on regional assessments of genetic structure and phylogeography. Here we synthesize available control region sequences for loggerhead turtles from the Mediterranean Sea, Atlantic, and western Indian Ocean basins. These data represent six of the nine globally significant regional management units (RMUs) for the species and include novel sequence data from Brazil, Cape Verde, South Africa and Oman. Genetic tests of differentiation among 42 rookeries represented by short sequences (380 bp haplotypes from 3,486 samples) and 40 rookeries represented by long sequences (∼800 bp haplotypes from 3,434 samples) supported the distinction of the six RMUs analyzed as well as recognition of at least 18 demographically independent management units (MUs) with respect to female natal homing. A total of 59 haplotypes were resolved. These haplotypes belonged to two highly divergent global lineages, with haplogroup I represented primarily by CC-A1, CC-A4, and CC-A11 variants and haplogroup II represented by CC-A2 and derived variants. Geographic distribution patterns of haplogroup II haplotypes and the nested position of CC-A11.6 from Oman among the Atlantic haplotypes invoke recent colonization of the Indian Ocean from the Atlantic for both global lineages. The haplotypes we confirmed for western Indian Ocean RMUs allow reinterpretation of previous mixed stock analysis and further suggest that contemporary migratory connectivity between the Indian and Atlantic Oceans occurs on a broader scale than previously hypothesized. This study represents a valuable model for conducting comprehensive international cooperative data management and research in marine ecology. 相似文献
93.
Paolo Guidetti Pasquale Baiata Enric Ballesteros Antonio Di Franco Bernat Hereu Enrique Macpherson Fiorenza Micheli Antonio Pais Pieraugusto Panzalis Andrew A. Rosenberg Mikel Zabala Enric Sala 《PloS one》2014,9(4)
Marine protected areas (MPAs) were acknowledged globally as effective tools to mitigate the threats to oceans caused by fishing. Several studies assessed the effectiveness of individual MPAs in protecting fish assemblages, but regional assessments of multiple MPAs are scarce. Moreover, empirical evidence on the role of MPAs in contrasting the propagation of non-indigenous-species (NIS) and thermophilic species (ThS) is missing. We simultaneously investigated here the role of MPAs in reversing the effects of overfishing and in limiting the spread of NIS and ThS. The Mediterranean Sea was selected as study area as it is a region where 1) MPAs are numerous, 2) fishing has affected species and ecosystems, and 3) the arrival of NIS and the northward expansion of ThS took place. Fish surveys were done in well-enforced no-take MPAs (HP), partially-protected MPAs (IP) and fished areas (F) at 30 locations across the Mediterranean. Significantly higher fish biomass was found in HP compared to IP MPAs and F. Along a recovery trajectory from F to HP MPAs, IP were similar to F, showing that just well enforced MPAs triggers an effective recovery. Within HP MPAs, trophic structure of fish assemblages resembled a top-heavy biomass pyramid. Although the functional structure of fish assemblages was consistent among HP MPAs, species driving the recovery in HP MPAs differed among locations: this suggests that the recovery trajectories in HP MPAs are likely to be functionally similar (i.e., represented by predictable changes in trophic groups, especially fish predators), but the specific composition of the resulting assemblages may depend on local conditions. Our study did not show any effect of MPAs on NIS and ThS. These results may help provide more robust expectations, at proper regional scale, about the effects of new MPAs that may be established in the Mediterranean Sea and other ecoregions worldwide. 相似文献
94.
Alan M. Friedlander David Obura Riaz Aumeeruddy Enric Ballesteros Julie Church Emma Cebrian Enric Sala 《PloS one》2014,9(1)
We report a reef ecosystem where corals may have lost their role as major reef engineering species but fish biomass and assemblage structure is comparable to unfished reefs elsewhere around the world. This scenario is based on an extensive assessment of the coral reefs of Farquhar Atoll, the most southern of the Seychelles Islands. Coral cover and overall benthic community condition at Farquhar was poor, likely due to a combination of limited habitat, localized upwelling, past coral bleaching, and cyclones. Farquhar Atoll harbors a relatively intact reef fish assemblage with very large biomass (3.2 t ha−1) reflecting natural ecological processes that are not influenced by fishing or other local anthropogenic factors. The most striking feature of the reef fish assemblage is the dominance by large groupers, snappers, and jacks with large (>1 m) potato cod (Epinephelus tukula) and marbled grouper (E. polyphekadion), commonly observed at many locations. Napoleon wrasse (Cheilinus undulatus) and bumphead parrotfish (Bolbometopon muricatum) are listed as endangered and vulnerable, respectively, but were frequently encountered at Farquhar. The high abundance and large sizes of parrotfishes at Farquhar also appears to regulate macroalgal abundance and enhance the dominance of crustose corallines, which are a necessary condition for maintenance of healthy reef communities. Overall fish biomass and biomass of large predators at Farquhar are substantially higher than other areas within the Seychelles, and are some of the highest recorded in the Indian Ocean. Remote islands like Farquhar Atoll with low human populations and limited fishing pressure offer ideal opportunities for understanding whether reefs can be resilient from global threats if local threats are minimized. 相似文献
95.
Estefanía Moreno Clara Andradas Mireia Medrano María M. Caffarel Eduardo Pérez-Gómez Sandra Blasco-Benito María Gómez-Ca?as M. Ruth Pazos Andrew J. Irving Carme Lluís Enric I. Canela Javier Fernández-Ruiz Manuel Guzmán Peter J. McCormick Cristina Sánchez 《The Journal of biological chemistry》2014,289(32):21960-21972
The G protein-coupled receptors CB2 (CB2R) and GPR55 are overexpressed in cancer cells and human tumors. Because a modulation of GPR55 activity by cannabinoids has been suggested, we analyzed whether this receptor participates in cannabinoid effects on cancer cells. Here we show that CB2R and GPR55 form heteromers in cancer cells, that these structures possess unique signaling properties, and that modulation of these heteromers can modify the antitumoral activity of cannabinoids in vivo. These findings unveil the existence of previously unknown signaling platforms that help explain the complex behavior of cannabinoids and may constitute new targets for therapeutic intervention in oncology. 相似文献
96.
Decline of Native Freshwater Fishes in a Mediterranean Watershed on the Iberian Peninsula: A Quantitative Assessment 总被引:2,自引:0,他引:2
Enric Aparicio Maria Josep Vargas Josep Maria Olmo Adolfo de Sostoa 《Environmental Biology of Fishes》2000,59(1):11-19
We examined changes in the distribution of 9 native and 18 introduced freshwater fishes in the south-eastern Pyrenees watershed, Iberian Peninsula, using data from 1996, 1984–1988 and historical information. This region suffers many modifications to its freshwater ecosystems that are linked to human activity in the Mediterranean regions. Fish communities, stream physical habitat and environmental degradation were assessed at 168 sites from 11 basins in 1996. Seven native species (78%) showed decline from previous data, one of which became extirpated in the first half of the 20th century. On the other hand, introduced species are expanding. As a consequence, intact native communities are increasingly rare, declining from presence in 22% of river courses in 1984–1988 to 15% in 1996. The most typical community type is a mixture of native and introduced species occupying 30% of river courses. Stream degradation seems to be the main cause of this process because fish communities differed between degraded streams and streams suffering less impact. A principal component analysis showed that water pollution and modifications to the habitat were the two anthropogenic factors that accounted for most changes in the fish community integrity. Habitat alteration, primarily through construction of dams and water diversions, has fragmented habitats and isolated native fish communities in headwater streams. Current protection measures do not offer effective conservation of threatened species and communities. A global conservation and restoration programme from an ecosystem-based approach is essential to reverse the trend affecting native freshwater fishes in this Mediterranean region. 相似文献
97.
98.
Marine ecosystems are diverse and complex, providing significant challenges to the development of generalizable metrics of
ecosystem health. Of particular concern is the varied form of change caused by multiple human activities, which limits the
capacity to generate a single measure to encapsulate the overall condition of the ecosystem. Here we consider how successional
theory can help to simplify our understanding of marine community structure, especially when viewed in context of human disturbance.
During succession, the emergent properties of communities change in predictable ways. As communities mature, there is an increase
in total production and biomass, the mean size of organisms, the level of internal recycling of food and nutrients, and the
mean trophic level. Using a set of multi-species trophic models, we explore the changes in community structure that are likely
to occur during succession. These changes include increases in biomass within trophic levels due to decreased rates of energy
and food loss through trophic and production inefficiencies, and potential shifts from top-down control early in succession
to bottom-up later. Because human activities disproportionately favor early-successional species, we can gain insights by
considering community degradation in the context of succession being played in reverse. Indicators of health based on ecological
succession thus provide a mechanistic view to measure the impact of human activities (both positive and negative) on marine
ecosystems. 相似文献
99.
The eosinophil cationic protein (ECP) is a human antimicrobial protein involved in the host immune defense that belongs to the pancreatic RNase A family. ECP displays a wide range of antipathogen activities. The protein is highly cationic and its bactericidal activity is dependant on both cationic and hydrophobic surface exposed residues. Previous studies on ECP by site-directed mutagenesis indicated that the RNase activity is not essential for its bactericidal activity. To further understand the ECP bactericidal mechanism, we have applied enzymatic and chemical limited cleavage to search for active sequence determinants.Following a search for potential peptidases we selected the Lys-endoproteinase, which cleaves the ECP polypeptide at the carboxyl side of its unique Lys residue, releasing the N-terminal fragment (0-38).Chemical digestion using cyanogen bromide released several complementary peptides at the protein N-terminus. Interestingly, ECP treatment with cyanogen bromide represents a new example of selective chemical cleavage at the carboxyl side of not only Met but also Trp residues. Recombinant ECP was denatured and carboxyamidomethylated prior to enzymatic and chemical cleavage. Irreversible denaturation abolishes the protein bactericidal activity.The characterization of the digestion products by both enzymatic and chemical approaches identifies a region at the protein N-terminus, from residues 11 to 35, that retains the bactericidal activity. The most active fragment, ECP(0-38), is further compared to ECP derived synthetic peptides. The region includes previously identified stretches related to lipopolysaccharide binding and bacteria agglutination. The results contribute to define the shortest ECP minimized version that would retain its antimicrobial properties. The data suggest that the antimicrobial RNase can provide a scaffold for the selective release of cytotoxic peptides. 相似文献
100.
Pla M La Paz JL Peñas G García N Palaudelmàs M Esteve T Messeguer J Melé E 《Transgenic research》2006,15(2):219-228
Maize is one of the main crops worldwide and an increasing number of genetically modified (GM) maize varieties are cultivated
and commercialized in many countries in parallel to conventional crops. Given the labeling rules established e.g. in the European
Union and the necessary coexistence between GM and non-GM crops, it is important to determine the extent of pollen dissemination
from transgenic maize to other cultivars under field conditions. The most widely used methods for quantitative detection of
GMO are based on real-time PCR, which implies the results are expressed in genome percentages (in contrast to seed or grain
percentages). Our objective was to assess the accuracy of real-time PCR based assays to accurately quantify the contents of
transgenic grains in non-GM fields in comparison with the real cross-fertilization rate as determined by phenotypical analysis.
We performed this study in a region where both GM and conventional maize are normally cultivated and used the predominant
transgenic maize Mon810 in combination with a conventional maize variety which displays the characteristic of white grains
(therefore allowing cross-pollination quantification as percentage of yellow grains). Our results indicated an excellent correlation
between real-time PCR results and number of cross-fertilized grains at Mon810 levels of 0.1–10%. In contrast, Mon810 percentage
estimated by weight of grains produced less accurate results. Finally, we present and discuss the pattern of pollen-mediated
gene flow from GM to conventional maize in an example case under field conditions. 相似文献