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81.
82.
Michael L. English Charles H. Stammer 《Biochemical and biophysical research communications》1978,83(4):1464-1467
A pentapeptide, Z-Gly-Gly-Phe-Phe-Ala · OH and the corresponding unsaturated pentapeptide, Z-Gly-Gly-Phe-ΔZPhe-Ala · OH , have been synthesized. The saturated compound was rapidly hydrolyzed by both chymotrypsin and thermolysin to the expected products, but the dehydropeptide was completely unhydrolyzed by either enzyme even after thirty hours. A new method of peptide stabilization to enzymolysis is made available. 相似文献
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The sequential unfolding events of horse, cow, and tuna ferricytochromes c (cyt c) as a function of increasing temperature over the range 25-81 degrees C were investigated by resolution-enhanced two-dimensional infrared (2D IR) correlation spectroscopy. The 2D IR analysis revealed that in the thermal denaturation of the two mammalian cyts, the overall sequence of unfolding is similar, with denaturation of extended-chain and turn structures occurring prior to unfolding of alpha-helices, followed by denaturation of residual stable extended-chain structures. In tuna cyt c, denaturation of all extended-chain structures precedes the unfolding of alpha-helices. Moreover, in cow cyt c, unfolding of all helical components occurs as one cooperative unit, but in horse and tuna cyts c, the helical components behave as subdomains that unfold separately, as proposed recently by Englander and co-workers for horse cyt c [Bai et al. (1995) Science 269, 192-197; Milne et al. (1999) J. Mol. Biol. 290, 811-822]. At higher temperatures, following the loss of secondary structure, protein aggregation occurs in the three cyts c. The data presented here establish that variations in the thermal unfolding of cyts c can be associated with specific sites in the protein that influence local flexibility yet have little affect on global stability. This study demonstrates the power of resolution-enhanced 2D IR correlation spectroscopy in probing unfolding events in homologous proteins. 相似文献
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Linsen Li Antony Okumu Sheri Dellos-Nolan Zoe Li Soumendrakrishna Karmahapatra Anthony English Jack C. Yalowich Daniel J. Wozniak Mark J. Mitton-Fry 《Bioorganic & medicinal chemistry letters》2018,28(14):2477-2480
Novel bacterial type II topoisomerase inhibitors (NBTIs) constitute a promising new class of antibacterial agents. We report a series of NBTIs with potent anti-staphylococcal activity and diminished hERG inhibition. Dioxane-linked compound 9 demonstrated MICs ≤1?μg/mL against both methicillin-susceptible (MSSA) and -resistant Staphylococcus aureus (MRSA), accompanied by reduced hERG inhibition as compared to cyclohexane- or piperidine-linked analogs. 相似文献
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Lucas A. Cernusak Margaret M. Barbour Stefan K. Arndt Alexander W. Cheesman Nathan B. English Taylor S. Feild Brent R. Helliker Meisha M. Holloway‐Phillips Joseph A.M. Holtum Ansgar Kahmen Francesca A. McInerney Niels C. Munksgaard Kevin A. Simonin Xin Song Hilary Stuart‐Williams Jason B. West Graham D. Farquhar 《Plant, cell & environment》2016,39(5):1087-1102
Leaf water contains naturally occurring stable isotopes of oxygen and hydrogen in abundances that vary spatially and temporally. When sufficiently understood, these can be harnessed for a wide range of applications. Here, we review the current state of knowledge of stable isotope enrichment of leaf water, and its relevance for isotopic signals incorporated into plant organic matter and atmospheric gases. Models describing evaporative enrichment of leaf water have become increasingly complex over time, reflecting enhanced spatial and temporal resolution. We recommend that practitioners choose a model with a level of complexity suited to their application, and provide guidance. At the same time, there exists some lingering uncertainty about the biophysical processes relevant to patterns of isotopic enrichment in leaf water. An important goal for future research is to link observed variations in isotopic composition to specific anatomical and physiological features of leaves that reflect differences in hydraulic design. New measurement techniques are developing rapidly, enabling determinations of both transpired and leaf water δ18O and δ2H to be made more easily and at higher temporal resolution than previously possible. We expect these technological advances to spur new developments in our understanding of patterns of stable isotope fractionation in leaf water. 相似文献
90.
Angelo Filosa A. A. Ismail A. M. English 《Journal of biological inorganic chemistry》1999,4(6):717-726
Fourier transform infrared (FTIR) spectroscopy is used to compare the thermally induced conformational changes in horse,
bovine and tuna ferricytochromes c in 50 mM phosphate/0.2 M KCl. Thermal titration in D2O at pD 7.0 of the amide II intensity of the buried peptide NH protons reveals tertiary structural transitions at 54 °C in horse and at 57 °C in bovine c. These transitions, which occur well before loss of secondary structure, are associated with the alkaline isomerization involving
Met80 heme-ligand exchange. In tuna c, the amide-II-monitored alkaline isomerization occurs at 35 °C, followed by a second amide II transition at 50 °C revealing
a hitherto unreported conformational change in this cytochrome. Amide II transitions at 50 °C (tuna) and 54 °C (horse) are
also observed during the thermal titration of the CN–-ligated cytochromes (where CN– displaces the Met80 ligand), but a well-defined 35 °C amide II transition is absent from the titration curve of the CN–adduct of tuna c. The different mechanisms suggested by the FTIR data for the alkaline isomerization of tuna and the mammalian cytochromes c are discussed. After the alkaline isomerization, loss of secondary structure and protein aggregation occur within a 5 °C
range with T
m values at 74 °C (bovine c), 70 °C (horse c) and 65 °C (tuna c), as monitored by changes in the amide I′ bands. The FTIR spectra were also used to compare the secondary structures of the
ferricytochromes c at 25 °C. Curve fitting of the amide I (H2O) and amide I′ (D2O) bands reveals essentially identical secondary structure in horse and bovine c, whereas splitting of the α-helical absorption of tuna c indicates the presence of less-stable helical structures. CN– adduct formation results in no FTIR-detectable changes in the secondary structures of either tuna or horse c, indicating that Met80 ligation does not influence the secondary structural elements in these cytochromes. The data provided
here demonstrate for the first time that the selective thermal titration of the amide II intensity of buried peptide NH protons in D2O is a powerful tool in protein conformational analysis.
Received: 1 April 1999 / Accepted: 24 August 1999 相似文献