全文获取类型
收费全文 | 78865篇 |
免费 | 6535篇 |
国内免费 | 4861篇 |
专业分类
90261篇 |
出版年
2024年 | 138篇 |
2023年 | 901篇 |
2022年 | 2075篇 |
2021年 | 3602篇 |
2020年 | 2326篇 |
2019年 | 2838篇 |
2018年 | 2868篇 |
2017年 | 2030篇 |
2016年 | 2872篇 |
2015年 | 4585篇 |
2014年 | 5294篇 |
2013年 | 5961篇 |
2012年 | 6898篇 |
2011年 | 6352篇 |
2010年 | 3818篇 |
2009年 | 3373篇 |
2008年 | 4114篇 |
2007年 | 3652篇 |
2006年 | 3173篇 |
2005年 | 2679篇 |
2004年 | 2276篇 |
2003年 | 1972篇 |
2002年 | 1730篇 |
2001年 | 1559篇 |
2000年 | 1565篇 |
1999年 | 1447篇 |
1998年 | 847篇 |
1997年 | 797篇 |
1996年 | 808篇 |
1995年 | 736篇 |
1994年 | 687篇 |
1993年 | 530篇 |
1992年 | 818篇 |
1991年 | 657篇 |
1990年 | 601篇 |
1989年 | 531篇 |
1988年 | 421篇 |
1987年 | 362篇 |
1986年 | 336篇 |
1985年 | 299篇 |
1984年 | 221篇 |
1983年 | 199篇 |
1982年 | 112篇 |
1981年 | 118篇 |
1980年 | 86篇 |
1979年 | 147篇 |
1978年 | 84篇 |
1977年 | 95篇 |
1975年 | 111篇 |
1974年 | 116篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
151.
E Hu K Watkins S Groshen S C Chen B Malloy R Agah P Nichols J Parker A Martin M G Boosalis 《Molecular biotherapy》1990,2(2):96-103
A phase I trial of interleukin-2 and interferon gamma combination treatment in patients with advanced malignancies was performed based on preclinical in vitro and in vivo data which demonstrated synergistic antitumor effect. The toxicities, immune parameters, and tumor responses are described. The clinical and biologic maximal tolerated doses were extrapolated from these data. 相似文献
152.
小麦—天兰偃麦草—黑麦三属杂种花粉植株诱导条件的研究 总被引:3,自引:1,他引:2
研究表明,三属杂种处于单核中晚期阶段的花粉最适于诱导形成愈伤组织。低温预处理对促进三属杂种花粉愈伤组织的诱导有一定的作用。利用以马铃薯提取物为基础物质的马铃薯-Ⅱ培养基作诱导培养基,其愈伤组织诱导与分化的频率比目前两个较好的合成培养基要高。同一个三属杂种F_1春、秋播种植株之间在形成愈伤组织的能力上有较大的差异,秋播材料形成愈伤组织的能力明显高于春播材料。F_(?)杂种植株诱导愈伤组织和分化植株的频率均比F_1杂种明显提高。 相似文献
153.
154.
155.
Ligand-induced endocytosis of the EGF receptor is blocked by mutational inactivation and by microinjection of anti-phosphotyrosine antibodies 总被引:29,自引:0,他引:29
Early events in ligand-induced endocytosis of the EGF receptor have been examined. A mutant EGF receptor devoid of intrinsic protein-tyrosine kinase activity bound EGF and dimerized normally yet failed to undergo ligand-induced internalization. Immunofluorescence microscopy revealed that receptors lacking kinase activity failed to undergo the ligand-induced internalization characteristic of receptors with kinase activity. Monoclonal anti-phosphotyrosine antibodies effectively inhibited phosphorylation of exogenous substrates in vitro and, when microinjected into cells containing active EGF receptors, prevented internalization of the receptor when cells were subsequently challenged with EGF. These results point to a crucial role for the kinase activity of the EGF receptor in the process of ligand-induced endocytosis of receptors, and imply that a phosphorylated substrate(s) is required. 相似文献
156.
157.
An altered pattern of cross-resistance in multidrug-resistant human cells results from spontaneous mutations in the mdr1 (P-glycoprotein) gene 总被引:27,自引:0,他引:27
Multidrug resistance in human cells results from increased expression of the mdr1 (P-glycoprotein) gene. Although the same gene is activated in cells selected with different drugs, multidrug-resistant cell lines can be preferentially resistant to their selecting agent. The mdr1 cDNA sequence from vinblastine-selected KB cells, which are uniformly resistant to different lipophilic drugs, was compared with the corresponding sequence from colchicine-selected KB cells preferentially resistant to colchicine. These sequences differ at three positions, resulting in a single amino acid change in P-glycoprotein. These differences result from mutations that occurred during colchicine selection. The appearance of these mutations coincides with the emergence of preferential resistance to colchicine. We have constructed biologically active mdr1 cDNA clones that express either wild-type or mutant P-glycoprotein. Multi-drug-resistant transfectants obtained with the mutant sequence were characterized by increased relative resistance to colchicine compared with transfectants obtained with wild-type sequence. mdr1 mutations are therefore responsible for preferential resistance to colchicine in multidrug-resistant KB cells. 相似文献
158.
159.
A d-aminoacylase-producing microorganism, strain DA181, isolated from soil was identified as Alcaligenes denitrificans subsp. denitrificans. This strain produced about 29,300 units (micromoles of product formed per hour) of d-aminoacylase and 2,300 units of l-aminoacylase per gram of cells (wet weight) when cultivated in a medium containing 1% N-acetyl-dl-leucine as the carbon source. The d-aminoacylase was purified 345-fold. The specific activity of the purified enzyme was 108,600 units per mg of protein when N-acetyl-d-methionine was used as a substrate. The apparent molecular weight was 58,000, as estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. N-Acetyl-d-methionine was the favored substrate, followed by N-acetyl-d-phenylalanine. This enzyme had a high stereospecificity, and its hydrolysis of N-acetyl-l-amino acids was almost negligible. 相似文献
160.
Wei de Zhang Fan Qing Li Xiao Yun Zhang Ying Chen 《Biological trace element research》1988,17(1):81-90
The growth of the protozoanBlepherisma is stimulated by Lanthanum (La) at concentrations as low as 0.32 ppm. In mice Yttrium (Y) and Ytterbium (Yb) are absorbed,
accumulated, and metabolized. Both rare earth elements (RE) exhibit a high affinity for teeth and bones, accumulation occurs
and metabolism is slow. In the livers of RE-exposed mice, concentrations are variable. The liver is apparently capable of
absorbing and discharging RE in a manner depending on metabolic activity. The main route of discharge for ingested REs is
the alimentary canal. Exposure of pregnant mice to RE leads to rapid placental transfer of RE; 14.1% of the total amount of
RE administered was detected in newborn mice. Young, developing organisms appear to be especially susceptible to RE accumulation. 相似文献