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71.
J Pan Q Chen D Willenbring D Mowrey XP Kong A Cohen CB Divito Y Xu P Tang 《Structure (London, England : 1993)》2012,20(9):1463-1469
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72.
Tengku Shahrul Anuar Hesham M. Al-Mekhlafi Mohamed Kamel Abdul Ghani Emelia Osman Azlin Mohd Yasin Anisah Nordin Siti Nor Azreen Fatmah Md Salleh Nuraffini Ghazali Mekadina Bernadus Norhayati Moktar 《PloS one》2012,7(10)
Background
Entamoeba histolytica/Entamoeba dispar/Entamoeba moshkovskii infection is still prevalent in rural Malaysia especially among Orang Asli communities. Currently, information on prevalence of this infection among different ethnic groups of Orang Asli is unavailable in Malaysia. To contribute to a better comprehension of the epidemiology of this infection, a cross-sectional study aimed at providing the first documented data on the prevalence and risk factors associated with E. histolytica/E. dispar/E. moshkovskii infection was carried out among three Orang Asli ethnic groups (Proto-Malay, Negrito, and Senoi) in selected villages in Negeri Sembilan, Perak, and Pahang states, Malaysia.Methods/Findings
Faecal samples were examined by formalin-ether sedimentation and trichrome staining techniques. Of 500 individuals, 8.7% (13/150) of Proto-Malay, 29.5% (41/139) of Negrito, and 18.5% (39/211) of Senoi were positive for E. histolytica/E. dispar/E. moshkovskii, respectively. The prevalence of this infection showed an age-dependency relationship, with higher rates observed among those aged less than 15 years in all ethnic groups studied. Multivariate analysis confirmed that not washing hands after playing with soils or gardening and presence of other family members infected with E. histolytica/E. dispar/E. moshkovskii were significant risk factors of infection among all ethnic groups. However, eating with hands, the consumption of raw vegetables, and close contact with domestic animals were identified as significant risk factors in Senoi.Conclusions
Essentially, the findings highlighted that E. histolytica/E. dispar/E. moshkovskii parasites are still prevalent in Malaysia. Further studies using molecular approaches to distinguish the morphologically identical species of pathogenic, E. histolytica from the non-pathogenic, E. dispar and E. moshkovskii are needed. The establishment of such data will be beneficial for the public health authorities in the planning and implementation of specific prevention and control strategies of this infection in different Orang Asli ethnic groups in Malaysia. 相似文献73.
74.
Aina Hazimah Bahaman Azzmer Azzar Abdul Hamid Khairul Bariyyah Abd Halim Yilmaz Kaya Mohamed Faraj Edbeib 《Journal of biomolecular structure & dynamics》2020,38(14):4246-4258
Abstract Fungi of the Trichoderma species are valued industrial enzymes in support of the ‘zero-waste’ technology to convert agro-industrial biomass into valuable products, i.e. nanocellulose (NC). In this study, an in silico approach using substrate docking and molecular dynamic (MD) simulation was used to predict the order of which the multilayers of cellulosic polymers, i.e. lignin, hemicellulose and cellulose in oil palm leaves (OPL) are degraded by fungal enzymes, endocellulase and exocellulase. The study aimed to establish the catalytic tendencies of the enzymes to optimally degrade the cellulosic components of OPL for high yield production of NC. Energy minimized endocellulase and exocellulase models revealed satisfactory scores of PROCHECK (90.0% and 91.2%), Verify3D (97.23% and 98.85%) and ERRAT (95.24% and 91.00%) assessments. Active site prediction by blind docking, COACH meta-server and multiple sequence alignment indicated the catalytic triads for endocellulase and exocellulase were Ser116–His205–Glu249 and Ser382–Arg124–Asp385, respectively. Binding energy of endocellulase docked with hemicellulose (?6.0 ? kcal mol?1) was the most favourable followed by lignin (?5.6 ? kcal mol?1) and cellulose (?4.4 ? kcal mol?1). Exocellulase, contrarily, bonded favorably with lignin (?8.7 ? kcal mol?1), closely followed by cellulose (?8.5 ? kcal mol?1) and hemicellulose (?8.4 ? kcal mol?1). MDs simulations showed that interactions of complexes, endocellulase–hemicellulose and the exocellulase–cellulose being the most stable. Thus, the findings of the study successfully identified the specific actions of sugar-acting enzymes for NC production. Communicated by Ramaswamy H. Sarma 相似文献
75.
76.
Aina Gotoh Masafumi Hidaka Keiko Hirose Takafumi Uchida 《The Journal of biological chemistry》2013,288(48):34699-34706
Neurons undergo several morphological changes as a part of normal neuron maturation process. Alzheimer disease is associated with increased neuroproliferation and impaired neuronal maturation. In this study, we demonstrated that Gas7b (growth arrest specific protein 7b) expression in a neuronal cell line, Neuro 2A, induces cell maturation by facilitating formation of dendrite-like processes and/or filopodia projections and that Gas7b co-localizes with neurite microtubules. Molecular analysis was performed to evaluate whether Gas7b associates with actin filaments and microtubules, and the data revealed two novel roles of Gas7b in neurite outgrowth: we showed that Gas7b enhances bundling of several microtubule filaments and connects microtubules with actin filaments. These results suggest that Gas7b governs neural cell morphogenesis by enhancing the coordination between actin filaments and microtubules. We conclude that lower neuronal Gas7b levels may impact Alzheimer disease progression. 相似文献
77.
Purification and specificity of pig intestinal prolidase 总被引:5,自引:0,他引:5
78.
79.
Livia Garzia Noriyuki Kijima A. Sorana Morrissy Pasqualino De Antonellis Ana Guerreiro-Stucklin Borja L. Holgado Xiaochong Wu Xin Wang Michael Parsons Kory Zayne Alex Manno Claudia Kuzan-Fischer Carolina Nor Laura K. Donovan Jessica Liu Lei Qin Alexandra Garancher Kun-Wei Liu Michael D. Taylor 《Cell》2018,172(5):1050-1062.e14
80.
Multiple origins of stone loach,Barbatula barbatula (Teleostei: Nemacheilidae), in Sweden based on mitochondrial DNA
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M. Norén S. Kullander T. Nydén P. Johansson 《Zeitschrift fur angewandte Ichthyologie》2018,34(1):58-65
Stone loach, Barbatula barbatula, occurs in scattered localities in Sweden. Some of the populations have usually been considered as feral descendants of escaped 18th Century pond stock, but historical documentation is inconclusive. Using the mitochondrial COI gene as a marker, we analyzed specimens from seven Swedish localities. One of the middle Swedish localities, in Stockholm, belongs to a haplotype found also in Poland and Lithuania. Two other samples, from near Nyköping and Lake Hjälmaren, belong to a haplotype found in northeastern Europe (Finland, Estonia, Latvia, Volga basin in Russia). Those two Swedish populations are probably descendants from at least two introductions, probably for pond rearing for human consumption. Samples from Skåne and Halland in southern Sweden belong to the haplotype found in Denmark, northern Germany and Poland; and whereas it remains possible that they also represent feral populations, they may be naturally occurring, having reached Sweden during the Ancylus period, about 8,000–10,000 years ago. A recently discovered population from the central South Swedish Highlands belongs to a mainly southeastern European haplotype. It probably represents a release of imported aquarium specimens or live bait carried by sport fishing tourists. 相似文献