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Brian W. Parks Elizabeth Nam Elin Org Emrah Kostem Frode Norheim Simon T. Hui Calvin Pan Mete Civelek Christoph D. Rau Brian J. Bennett Margarete Mehrabian Luke K. Ursell Aiqing He Lawrence W. Castellani Bradley Zinker Mark Kirby Thomas A. Drake Christian A. Drevon Aldons J. Lusis 《Cell metabolism》2013,17(1):141-152
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Mehmet Kaya Nurcan Orhan Emrah Karabacak Metin Berkant Bahceci Nadir Arican Bulent Ahishali Gonul Kemikler Atilla Uslu Aydin Cevik Canan Ugur Yilmaz Mutlu Kucuk Candan Gürses 《Life sciences》2013,92(4-5):289-297
AimsThis study investigates the effects of vagus nerve stimulation (VNS) on seizure severity and blood–brain barrier (BBB) integrity in kindled rats with cortical dysplasia (CD).Main methodsPregnant rats were exposed to 145 cGy of gamma-irradiation on day 17 of pregnancy. In offsprings, kindling was induced by giving subconvulsive doses of pentylenetetrazole. Left VNS was performed for 48 h at output currents of 0.5 or 1 mA. Horseradish peroxidase (HRP) was used to study the BBB permeability. Immunohistochemistry for occludin and P-glycoprotein (P-gp) was also performed.Key findingsKindled rats with CD exhibited seizures with mean Racine's scores of 3.57 ± 1.2 during video EEG recording. Kindled animals with CD receiving VNS at 0.5 and 1.0 mA did not exhibit either clinical or electrophysiological signs of seizure. Immunostaining for occludin, a tight junction protein, in hippocampus remained relatively intact in all groups. VNS-treated and -untreated kindled animals with CD revealed intense immunostaining for P-gp in hippocampal formation (P < 0.01). Electron microscopic observations revealed frequent transport vesicles containing electron-dense HRP reaction products in the cytoplasm of brain capillary endothelial cells in both cerebral cortex and hippocampus of kindled animals with CD. Those which were exposed to 1 mA VNS were observed to have brain capillary endothelial cells largely devoid of HRP reaction products in both cerebral cortex and hippocampus.SignificanceThe results of this study suggest that VNS therapy at 1 mA inhibits seizure activity and protects BBB integrity by limiting the enhancement of transcellular pathway in kindled animals with CD. 相似文献
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Suleyman Önal Mustafa Nazıroğlu Mesut Çolak Vedat Bulut Manuel F. Flores-Arce 《Biological trace element research》2011,142(3):447-455
The aim of the present study was to measure the changes in serum selenium, zinc, and copper in patients being treated for
rheumatoid arthritis. Thirty-two patients and 52 healthy controls were included in the study. The copper level was higher
and those of selenium and zinc were lower in the patients relative to controls. Treatment with methotrexate elevated the zinc
levels, but not zinc and selenium. Treatments with salazopyrin, corticosteroids, chloroquine, and non-steroidal anti-inflammatory
drugs did not change the levels of any of the elements studied. The decrease in zinc and selenium levels and elevation in
copper levels observed in the patients probably resulted from the defense response of organism and are mediated by inflammatory-like
substances. 相似文献
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Cüneyt Çirak Jolita Radušienė Liudas Ivanauskas Valdimaras Janulis 《Acta Physiologiae Plantarum》2007,29(3):197-203
The genus Hypericum has received considerable interest from scientists, as it contains the variety of structurally diverse natural products which
possess a wide array of biological properties. The present study was conducted to determine ontogenetic and morphogenetic
variation of hypericin, chlorogenic acid and flavonoids, as rutin, hyperoside, apigenin-7-O-glucoside, quercitrin and quercetin content in Hypericum origanifolium growing in Turkey. Wild growing plants were harvested at vegetative, floral budding, full flowering, fresh fruiting and mature
fruiting stages and dissected into stem, leaf and reproductive tissues and assayed for bioactive compounds by HPLC method.
Hypericin, quercetin and quercitrin content in whole plant increased during course of ontogenesis and the highest level was
reached in blooming stage. On the contrary, hyperoside content of whole plant decreased linearly with advancing of development
stages and the highest level was observed at vegetative stage. Plants produced similar amount of chlorogenic acid at all stages
of plant phenology except for mature fruiting at which the amount of this compound was decreased sharply. Among different
tissues, reproductive parts accumulated the highest level of hypericin, quercetin and quercitrin, however, leaves produced
substantially higher amount of chlorogenic acid and hyperoside. Rutin and apigenin-7-O-glucoside were detectable in all tissues only during fruit maturation. The presence and variation of these bioactive substances
in H. origanifolium were reported for the first time. 相似文献
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Eren I Naziroğlu M Demirdaş A Celik O Uğuz AC Altunbaşak A Ozmen I Uz E 《Neurochemical research》2007,32(3):497-505
Venlafaxine is an approved antidepressant that is an inhibitor of both serotonin and norepinephrine transporters. Medical
treatment with oral venlafaxine can be beneficial to depression due to reducing free radical production in the brain and medulla
of depression- induced rats because oxidative stress may a play role in some depression. We investigated the effect of venlafaxine
administration and experimental depression on lipid peroxidation and antioxidant levels in cortex brain, medulla and erythrocytes
of rats. Thirty male wistar rats were used and were randomly divided into three groups. Venlafaxine (20 mg/kg) was orally
supplemented to depression-induced rats constituting the first group for four week. Second group was depression-induced group
although third group was used as control. Depressions in the first and second groups were induced on day zero of the study
by chronic mild stress. Brain, medulla and erythrocytes samples were taken from all animals on day 28. Depression resulted
in significant decrease in the glutathione peroxidase (GSH-Px) activity and vitamin C concentrations of cortex brain, glutathione
(GSH) value of medulla although their levels were increased by venlafaxine administration to the animals of depression group.
The lipid peroxidation levels in the three tissues and nitric oxide value in cortex brain elevated although their levels were
decreased by venlafaxine administration. There were no significant changes in cortex brain vitamin A, erythrocytes vitamin
C, GSH-Px and GSH, medulla vitamin A, GSH and GSH-Px values. In conclusion, cortex brain within the three tissues was most
affected by oxidative stress although there was the beneficial effect of venlafaxine in the brain of depression-induced rats
on investigated antioxidant defenses in the rat model. The treatment of depression by venlafaxine may also play a role in
preventing oxidative stress.
Abstract of the paper was submitted in 1st Ion Channels and Oxidative Stress Congress, 14–16 September 2006, Isparta, Turkey. 相似文献
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Characterization of humic materials extracted from hazelnut husk and hazelnut husk amended soils 总被引:1,自引:0,他引:1
This study was carried out to determine effects of composted hazelnut husk (CHH) on some chemical properties of soil and soil
humic acid (HA). Compost application increases organic matter (OM) content of the soil considerably, OM value of 3.18% became
3.89% in 3 years time interval. Before application of compost, the soil pH was found to be 5.37, while after compost application
it became 5.61. FTIR characteristics of humic acid/humic acid-like materials extracted from the original hazelnut husk, composted
hazelnut husk and composted hazelnut husk amended soil have been investigated. C and O content of humic acid-like/humic acid
materials were in the range of 41.4–50.8% and 37.8–50.5%, respectively. The N content of the humic acid/humic acid-like materials are in the expected range for humic materials which is 2–6%.
Comparison of FTIR spectra of hazelnut husk and composted hazelnut husk humic acid-like fractions shows that both exhibit
similar but not identical series of IR bands indicating the presence of the same functional groups in both samples. The only
difference in the spectra seemed to be a decrement in the peak intensities of composted sample compared to uncomposted one.
The similar differentiation of the intensities of IR bands of compost applied soil sample has also been observed. The FTIR
spectral results show that the characteristics of composted material tend to become similar to that of soil humic acids characteristics
in time. 相似文献
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Dinçer Ayaz Uwe Fritz Cemal Varol Tok Ahmet Mermer Murat Tosunoğlu Murat Afsar Kerim Çiçek 《Biologia》2007,62(2):225-227
Data on population size, adult sex ratio, body size and mass are provided for a population of the turtle Emys orbicularis near Pazara?aç (Afyonkarahisar/Turkey). Using the mark-recapture method (triple catch), a population size of 664 turtles was estimated (95% confidence interval, range 332–996), corresponding to a density of 83 turtles per hectare (range 41.5–124.5). The adult sex-ratio was significantly skewed in favor of males (2.02 males: 1 female; P < 0.001). Almost all recorded specimens were adult (98.1%). Mean straight carapace length (SCL) and body mass (BM) of adult turtles were: SCL = 128.65 mm, BM = 345 g for males (n = 168) and SCL = 135.37 mm, BM = 463 g for females (n = 83). 相似文献
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Muhammad?Sajid Hussain Agatino Battaglia Sandra Szczepanski Emrah Kaygusuz Mohammad?Reza Toliat Shin-ichi Sakakibara Janine Altmüller Holger Thiele Gudrun Nürnberg Shahida Moosa G?khan Yigit Filippo Beleggia Sigrid Tinschert Jill Clayton-Smith Pradeep Vasudevan Jill?E. Urquhart Dian Donnai Alan Fryer Ferda Percin Francesco Brancati Angus Dobbie Robert ?migiel Gabriele Gillessen-Kaesbach Bernd Wollnik Angelika?Anna Noegel William?G. Newman Peter Nürnberg 《American journal of human genetics》2014,95(5):622-632
Filippi syndrome is a rare, presumably autosomal-recessive disorder characterized by microcephaly, pre- and postnatal growth failure, syndactyly, and distinctive facial features, including a broad nasal bridge and underdeveloped alae nasi. Some affected individuals have intellectual disability, seizures, undescended testicles in males, and teeth and hair abnormalities. We performed homozygosity mapping and whole-exome sequencing in a Sardinian family with two affected children and identified a homozygous frameshift mutation, c.571dupA (p.Ile191Asnfs∗6), in CKAP2L, encoding the protein cytoskeleton-associated protein 2-like (CKAP2L). The function of this protein was unknown until it was rediscovered in mice as Radmis (radial fiber and mitotic spindle) and shown to play a pivotal role in cell division of neural progenitors. Sanger sequencing of CKAP2L in a further eight unrelated individuals with clinical features consistent with Filippi syndrome revealed biallelic mutations in four subjects. In contrast to wild-type lymphoblastoid cell lines (LCLs), dividing LCLs established from the individuals homozygous for the c.571dupA mutation did not show CKAP2L at the spindle poles. Furthermore, in cells from the affected individuals, we observed an increase in the number of disorganized spindle microtubules owing to multipolar configurations and defects in chromosome segregation. The observed cellular phenotypes are in keeping with data from in vitro and in vivo knockdown studies performed in human cells and mice, respectively. Our findings show that loss-of-function mutations in CKAP2L are a major cause of Filippi syndrome. 相似文献