全文获取类型
收费全文 | 15478篇 |
免费 | 1232篇 |
国内免费 | 743篇 |
出版年
2024年 | 32篇 |
2023年 | 207篇 |
2022年 | 506篇 |
2021年 | 928篇 |
2020年 | 501篇 |
2019年 | 622篇 |
2018年 | 582篇 |
2017年 | 459篇 |
2016年 | 682篇 |
2015年 | 1083篇 |
2014年 | 1170篇 |
2013年 | 1163篇 |
2012年 | 1523篇 |
2011年 | 1250篇 |
2010年 | 732篇 |
2009年 | 647篇 |
2008年 | 788篇 |
2007年 | 728篇 |
2006年 | 591篇 |
2005年 | 565篇 |
2004年 | 442篇 |
2003年 | 373篇 |
2002年 | 279篇 |
2001年 | 228篇 |
2000年 | 160篇 |
1999年 | 172篇 |
1998年 | 111篇 |
1997年 | 117篇 |
1996年 | 114篇 |
1995年 | 107篇 |
1994年 | 109篇 |
1993年 | 67篇 |
1992年 | 68篇 |
1991年 | 86篇 |
1990年 | 54篇 |
1989年 | 49篇 |
1988年 | 25篇 |
1987年 | 20篇 |
1986年 | 18篇 |
1985年 | 25篇 |
1984年 | 19篇 |
1983年 | 18篇 |
1982年 | 8篇 |
1981年 | 3篇 |
1980年 | 5篇 |
1978年 | 2篇 |
1976年 | 2篇 |
1973年 | 2篇 |
1912年 | 1篇 |
1907年 | 1篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
151.
Mu‐Wen Nie Ye‐Chen Han Zhu‐Jun Shen Hong‐Zhi Xie 《Journal of cellular and molecular medicine》2020,24(14):7915-7927
Sepsis is the most common cause of death in intensive care units. This study investigated the circular RNA (circRNA) and mRNA expression profiles and functional networks of the aortic tissue in sepsis. We established a lipopolysaccharide (LPS)‐induced rat sepsis model. High‐throughput sequencing was performed on the aorta tissue to identify differentially expressed (DE) circRNAs and mRNAs, which were validated by real‐time quantitative polymerase chain reaction (RT‐qPCR). Bioinformatic analysis was carried out and coding and non‐coding co‐expression (CNC) and competing endogenous RNA (ceRNA) regulatory networks were constructed to investigate the mechanisms. In total, 373 up‐regulated and 428 down‐regulated circRNAs and 2063 up‐regulated and 2903 down‐regulated mRNAs were identified. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses of mRNAs showed that the down‐regulated genes were mainly enriched in the process of energy generation. CNC and ceRNA regulatory networks were constructed with seven DE circRNAs. The results of functional enrichment analysis of CNC target genes revealed the important role of circRNAs in inflammatory response. The ceRNA network also highlighted the significant enrichment in calcium signalling pathway. Significant alterations in circRNAs and mRNAs were observed in the aortic tissue of septic rats. In addition, CNC and ceRNA networks were established. 相似文献
152.
Patricia Kabitzke Diana Morales Dansha He Kimberly Cox Jane Sutphen Lucinda Thiede Emily Sabath Taleen Hanania Barbara Biemans Daniela Brunner 《Genes, Brain & Behavior》2020,19(7)
Phenotyping mouse model systems of human disease has proven to be a difficult task, with frequent poor inter‐ and intra‐laboratory replicability, particularly in behavioral domains such as social and cognitive function. However, establishing robust animal model systems with strong construct validity is of fundamental importance as they are central tools for understanding disease pathophysiology and developing therapeutics. To complete our studies of mouse model systems relevant to autism spectrum disorder (ASD), we present a replication of the main findings from our two published studies of five genetic mouse model systems of ASD. To assess the intra‐laboratory robustness of previous results, we chose the two model systems that showed the greatest phenotypic differences, the Shank3/F and Cntnap2, and repeated assessments of general health, activity and social behavior. We additionally explored all five model systems in the same framework, comparing all results obtained in this three‐yearlong effort using informatics techniques to assess commonalities and differences. Our results showed high intra‐laboratory replicability of results, even for those with effect sizes that were not particularly large, suggesting that discrepancies in the literature may be dependent on subtle but pivotal differences in testing conditions, housing enrichment, or background strains and less so on the variability of the behavioral phenotypes. The overall informatics analysis suggests that in our behavioral assays we can separate the set of tested mouse model system into two main classes that in some aspects lie on opposite ends of the behavioral spectrum, supporting the view that autism is not a unitary concept. 相似文献
153.
Yutian Zou Shaoquan Zheng Xinpei Deng Anli Yang Yanan Kong Maryam Kohansal Xiaoqian Hu Xiaoming Xie 《Journal of cellular and molecular medicine》2020,24(17):9507-9517
The circular RNA, CDR1as/ciRS‐7, functions as a vital regulator in various cancers; however, the predictive value of CDR1as remains controversial. Therefore, a comprehensive analysis for clarifying the precise diagnostic and prognostic value of CDR1as in solid tumours is needed. A literature review of several databases was conducted for identifying potential studies. Pooled odds ratios (ORs) and hazard ratios (HRs) were used for evaluating the diagnostic accuracy variables and survival. Overall, 15 studies (1787 patients) and 11 studies (1578 patients) were included for diagnostic and prognostic outcome syntheses, respectively. Up‐regulated CDR1as expression was found to be correlated with worse clinicopathological characteristics, including the T status, N status, histological grade, TNM stage and distant metastasis. The synthesized sensitivity was 0.72 (95% confidence interval [CI], 0.65‐0.79), and the specificity was 0.80 (95% CI, 0.74‐0.86). The positive likelihood ratio (LR), negative LR and diagnostic odds ratio (DOR) were 3.70, 0.34 and 10.80, respectively. The area under the receiver operator characteristic curve was 0.84 (95% CI, 0.80‐0.87). In the pooled prognostic analysis, patients with high CDR1as expression had worse overall survival (HR = 2.40, P < 0.001) and disease‐free survival (HR = 1.74, P < 0.001). These results suggest that CDR1as is a reliable diagnostic and prognostic biomarker with high accuracy and efficiency, which may potentially facilitate clinical decisions on solid tumours in the future. 相似文献
154.
Xiaoli Hu Rui Li Yanqing Wu Yi Li Xingfeng Zhong Guanyinsheng Zhang Yanmin Kang Shuhua Liu Ling Xie Junming Ye Jian Xiao 《Journal of cellular and molecular medicine》2020,24(14):8166-8178
The application of growth factors (GFs) for treating chronic spinal cord injury (SCI) has been shown to promote axonal regeneration and functional recovery. However, direct administration of GFs is limited by their rapid degradation and dilution at the injured sites. Moreover, SCI recovery is a multifactorial process that requires multiple GFs to participate in tissue regeneration. Based on these facts, controlled delivery of multiple growth factors (GFs) to lesion areas is becoming an attractive strategy for repairing SCI. Presently, we developed a GFs‐based delivery system (called GFs‐HP) that consisted of basic fibroblast growth factor (bFGF), nerve growth factor (NGF) and heparin‐poloxamer (HP) hydrogel through self‐assembly mode. This GFs‐HP was a kind of thermosensitive hydrogel that was suitable for orthotopic administration in vivo. Meanwhile, a 3D porous structure of this hydrogel is commonly used to load large amounts of GFs. After single injection of GFs‐HP into the lesioned spinal cord, the sustained release of NGF and bFGF from HP could significantly improve neuronal survival, axon regeneration, reactive astrogliosis suppression and locomotor recovery, when compared with the treatment of free GFs or HP. Moreover, we also revealed that these neuroprotective and neuroregenerative effects of GFs‐HP were likely through activating the phosphatidylinositol 3 kinase and protein kinase B (PI3K/Akt) and mitogen‐activated protein kinase/extracellular signal‐regulated kinase (MAPK/ERK) signalling pathways. Overall, our work will provide an effective therapeutic strategy for SCI repair. 相似文献
155.
156.
Purinergic Signalling - The P2X7 receptor (P2X7R) is an ATP-gated cation channel with a critical role in many physiological and pathological processes, and shows prominent functional differences... 相似文献
157.
JingWei Gao WanBing He ChangMing Xie Ming Gao LeiYu Feng ZhaoYu Liu JingFeng Wang Hui Huang PinMing Liu 《Journal of cellular and molecular medicine》2020,24(23):13648
It remains unclear whether the necessity of calcified mellitus induced by high inorganic phosphate (Pi) is required and the roles of autophagy plays in aldosterone (Aldo)‐enhanced vascular calcification (VC) and vascular smooth muscle cell (VSMC) osteogenic differentiation. In the present study, we found that Aldo enhanced VC both in vivo and in vitro only in the presence of high Pi, alongside with increased expression of VSMC osteogenic proteins (BMP2, Runx2 and OCN) and decreased expression of VSMC contractile proteins (α‐SMA, SM22α and smoothelin). However, these effects were blocked by mineralocorticoid receptor inhibitor, spironolactone. In addition, the stimulatory effects of Aldo on VSMC calcification were further accelerated by the autophagy inhibitor, 3‐MA, and were counteracted by the autophagy inducer, rapamycin. Moreover, inhibiting adenosine monophosphate‐activated protein kinase (AMPK) by Compound C attenuated Aldo/MR‐enhanced VC. These results suggested that Aldo facilitates high Pi‐induced VSMC osteogenic phenotypic switch and calcification through MR‐mediated signalling pathways that involve AMPK‐dependent autophagy, which provided new insights into Aldo excess‐associated VC in various settings. 相似文献
158.
159.
Xiao Wang Haiyun Xie Yufan Ying Danni Chen Jiangfeng Li 《Journal of cellular and molecular medicine》2020,24(18):10302-10310
Epigenetics has long been a hot topic in the field of scientific research. The scope of epigenetics usually includes chromatin remodelling, DNA methylation, histone modifications, non‐coding RNAs and RNA modifications. In recent years, RNA modifications have emerged as important regulators in a variety of physiological processes and in disease progression, especially in human cancers. Among the various RNA modifications, m6A is the most common. The function of m6A modifications is mainly regulated by 3 types of proteins: m6A methyltransferases (writers), m6A demethylases (erasers) and m6A‐binding proteins (readers). In this review, we focus on RNA m6A modification and its relationship with urological cancers, particularly focusing on its roles and potential clinical applications. 相似文献
160.
Covalent–organic frameworks (COFs), featuring structural diversity, framework tunability and functional versatility, have emerged as promising organic electrode materials for rechargeable batteries and garnered tremendous attention in recent years. The adjustable pore configuration, coupled with the functionalization of frameworks through pre‐ and post‐synthesis strategies, enables a precise customization of COFs, which provides a novel perspective to deepen the understanding of the fundamental problems of organic electrode materials. In this review, a summary of the recent research into COFs electrode materials for rechargeable batteries including lithium‐ion batteries, sodium‐ion batteries, potassium‐ion batteries, and aqueous zinc batteries is provided. In addition, this review will also cover the working principles, advantages and challenges, strategies to improve electrochemical performance, and applications of COFs in rechargeable batteries. 相似文献